Amino acid dipepetide frequency for Brevibacillus phage Abouo

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.149AlaAla: 3.149 ± 0.542
0.429AlaCys: 0.429 ± 0.17
3.65AlaAsp: 3.65 ± 0.527
4.796AlaGlu: 4.796 ± 0.637
4.08AlaPhe: 4.08 ± 0.572
4.008AlaGly: 4.008 ± 0.486
0.716AlaHis: 0.716 ± 0.257
4.652AlaIle: 4.652 ± 0.593
5.869AlaLys: 5.869 ± 0.528
6.585AlaLeu: 6.585 ± 0.877
2.004AlaMet: 2.004 ± 0.378
3.507AlaAsn: 3.507 ± 0.477
1.646AlaPro: 1.646 ± 0.546
1.789AlaGln: 1.789 ± 0.444
2.791AlaArg: 2.791 ± 0.441
3.865AlaSer: 3.865 ± 0.477
4.509AlaThr: 4.509 ± 0.61
4.581AlaVal: 4.581 ± 0.637
1.288AlaTrp: 1.288 ± 0.334
3.006AlaTyr: 3.006 ± 0.473
0.0AlaXaa: 0.0 ± 0.0
Cys
0.358CysAla: 0.358 ± 0.177
0.215CysCys: 0.215 ± 0.142
0.429CysAsp: 0.429 ± 0.178
0.787CysGlu: 0.787 ± 0.248
0.429CysPhe: 0.429 ± 0.17
0.286CysGly: 0.286 ± 0.17
0.286CysHis: 0.286 ± 0.15
0.501CysIle: 0.501 ± 0.219
0.286CysLys: 0.286 ± 0.149
0.358CysLeu: 0.358 ± 0.167
0.358CysMet: 0.358 ± 0.167
0.358CysAsn: 0.358 ± 0.154
0.072CysPro: 0.072 ± 0.065
0.358CysGln: 0.358 ± 0.168
0.573CysArg: 0.573 ± 0.218
0.429CysSer: 0.429 ± 0.218
0.286CysThr: 0.286 ± 0.136
0.573CysVal: 0.573 ± 0.206
0.215CysTrp: 0.215 ± 0.161
0.358CysTyr: 0.358 ± 0.168
0.0CysXaa: 0.0 ± 0.0
Asp
3.65AspAla: 3.65 ± 0.52
0.358AspCys: 0.358 ± 0.161
3.579AspAsp: 3.579 ± 0.528
6.37AspGlu: 6.37 ± 0.708
2.362AspPhe: 2.362 ± 0.415
4.08AspGly: 4.08 ± 0.499
0.573AspHis: 0.573 ± 0.207
4.438AspIle: 4.438 ± 0.524
4.867AspLys: 4.867 ± 0.627
4.939AspLeu: 4.939 ± 0.551
1.718AspMet: 1.718 ± 0.325
2.935AspAsn: 2.935 ± 0.435
2.147AspPro: 2.147 ± 0.408
2.076AspGln: 2.076 ± 0.317
2.362AspArg: 2.362 ± 0.38
3.006AspSer: 3.006 ± 0.454
2.791AspThr: 2.791 ± 0.42
3.865AspVal: 3.865 ± 0.587
0.93AspTrp: 0.93 ± 0.247
2.791AspTyr: 2.791 ± 0.416
0.0AspXaa: 0.0 ± 0.0
Glu
5.225GluAla: 5.225 ± 0.484
1.145GluCys: 1.145 ± 0.36
4.366GluAsp: 4.366 ± 0.589
6.728GluGlu: 6.728 ± 0.757
3.221GluPhe: 3.221 ± 0.466
4.151GluGly: 4.151 ± 0.517
1.789GluHis: 1.789 ± 0.289
6.585GluIle: 6.585 ± 0.692
7.015GluLys: 7.015 ± 0.695
7.086GluLeu: 7.086 ± 0.639
2.29GluMet: 2.29 ± 0.414
2.72GluAsn: 2.72 ± 0.327
1.503GluPro: 1.503 ± 0.323
3.937GluGln: 3.937 ± 0.527
5.44GluArg: 5.44 ± 0.677
4.151GluSer: 4.151 ± 0.559
3.149GluThr: 3.149 ± 0.631
6.728GluVal: 6.728 ± 0.77
1.074GluTrp: 1.074 ± 0.295
2.362GluTyr: 2.362 ± 0.37
0.0GluXaa: 0.0 ± 0.0
Phe
2.219PheAla: 2.219 ± 0.367
0.143PheCys: 0.143 ± 0.108
3.579PheAsp: 3.579 ± 0.469
2.935PheGlu: 2.935 ± 0.561
2.219PhePhe: 2.219 ± 0.532
2.29PheGly: 2.29 ± 0.335
0.501PheHis: 0.501 ± 0.221
3.149PheIle: 3.149 ± 0.465
3.364PheLys: 3.364 ± 0.512
3.006PheLeu: 3.006 ± 0.463
1.074PheMet: 1.074 ± 0.265
1.002PheAsn: 1.002 ± 0.307
1.432PhePro: 1.432 ± 0.331
1.503PheGln: 1.503 ± 0.323
2.004PheArg: 2.004 ± 0.538
2.577PheSer: 2.577 ± 0.422
2.076PheThr: 2.076 ± 0.391
3.293PheVal: 3.293 ± 0.476
0.429PheTrp: 0.429 ± 0.16
1.503PheTyr: 1.503 ± 0.311
0.0PheXaa: 0.0 ± 0.0
Gly
3.579GlyAla: 3.579 ± 0.669
0.573GlyCys: 0.573 ± 0.199
3.579GlyAsp: 3.579 ± 0.362
4.295GlyGlu: 4.295 ± 0.525
2.147GlyPhe: 2.147 ± 0.443
2.362GlyGly: 2.362 ± 0.413
0.644GlyHis: 0.644 ± 0.265
3.794GlyIle: 3.794 ± 0.47
6.156GlyLys: 6.156 ± 0.619
4.295GlyLeu: 4.295 ± 0.537
2.434GlyMet: 2.434 ± 0.356
3.722GlyAsn: 3.722 ± 0.583
0.787GlyPro: 0.787 ± 0.219
2.076GlyGln: 2.076 ± 0.393
3.364GlyArg: 3.364 ± 0.447
3.794GlySer: 3.794 ± 0.431
3.937GlyThr: 3.937 ± 0.505
4.581GlyVal: 4.581 ± 0.56
0.716GlyTrp: 0.716 ± 0.314
2.863GlyTyr: 2.863 ± 0.468
0.0GlyXaa: 0.0 ± 0.0
His
1.36HisAla: 1.36 ± 0.339
0.215HisCys: 0.215 ± 0.132
1.217HisAsp: 1.217 ± 0.274
1.074HisGlu: 1.074 ± 0.297
0.787HisPhe: 0.787 ± 0.212
0.859HisGly: 0.859 ± 0.222
0.286HisHis: 0.286 ± 0.169
1.288HisIle: 1.288 ± 0.289
0.573HisLys: 0.573 ± 0.159
1.002HisLeu: 1.002 ± 0.252
0.286HisMet: 0.286 ± 0.14
0.716HisAsn: 0.716 ± 0.199
0.429HisPro: 0.429 ± 0.155
0.573HisGln: 0.573 ± 0.173
0.501HisArg: 0.501 ± 0.155
0.644HisSer: 0.644 ± 0.238
1.217HisThr: 1.217 ± 0.278
0.573HisVal: 0.573 ± 0.21
0.429HisTrp: 0.429 ± 0.155
1.074HisTyr: 1.074 ± 0.325
0.0HisXaa: 0.0 ± 0.0
Ile
5.655IleAla: 5.655 ± 0.637
0.429IleCys: 0.429 ± 0.182
4.008IleAsp: 4.008 ± 0.494
6.728IleGlu: 6.728 ± 0.673
1.646IlePhe: 1.646 ± 0.383
4.08IleGly: 4.08 ± 0.499
1.002IleHis: 1.002 ± 0.238
4.438IleIle: 4.438 ± 0.798
6.943IleLys: 6.943 ± 0.62
5.941IleLeu: 5.941 ± 0.749
1.217IleMet: 1.217 ± 0.285
3.865IleAsn: 3.865 ± 0.491
3.149IlePro: 3.149 ± 0.472
2.577IleGln: 2.577 ± 0.447
3.364IleArg: 3.364 ± 0.459
5.44IleSer: 5.44 ± 0.678
3.937IleThr: 3.937 ± 0.555
4.151IleVal: 4.151 ± 0.409
0.429IleTrp: 0.429 ± 0.164
2.505IleTyr: 2.505 ± 0.506
0.0IleXaa: 0.0 ± 0.0
Lys
6.156LysAla: 6.156 ± 0.641
0.573LysCys: 0.573 ± 0.209
3.794LysAsp: 3.794 ± 0.61
7.086LysGlu: 7.086 ± 0.638
2.505LysPhe: 2.505 ± 0.397
5.798LysGly: 5.798 ± 0.575
1.145LysHis: 1.145 ± 0.255
5.225LysIle: 5.225 ± 0.595
8.16LysLys: 8.16 ± 0.855
6.513LysLeu: 6.513 ± 0.572
2.29LysMet: 2.29 ± 0.417
4.151LysAsn: 4.151 ± 0.612
3.006LysPro: 3.006 ± 0.437
4.008LysGln: 4.008 ± 0.525
4.724LysArg: 4.724 ± 0.587
3.865LysSer: 3.865 ± 0.621
5.297LysThr: 5.297 ± 0.489
4.796LysVal: 4.796 ± 0.62
0.859LysTrp: 0.859 ± 0.272
3.579LysTyr: 3.579 ± 0.569
0.0LysXaa: 0.0 ± 0.0
Leu
7.229LeuAla: 7.229 ± 0.759
0.787LeuCys: 0.787 ± 0.255
5.941LeuAsp: 5.941 ± 0.588
6.943LeuGlu: 6.943 ± 0.805
3.65LeuPhe: 3.65 ± 0.507
4.509LeuGly: 4.509 ± 0.478
1.074LeuHis: 1.074 ± 0.295
5.726LeuIle: 5.726 ± 0.683
5.44LeuLys: 5.44 ± 0.79
6.871LeuLeu: 6.871 ± 0.675
1.575LeuMet: 1.575 ± 0.236
4.796LeuAsn: 4.796 ± 0.511
2.577LeuPro: 2.577 ± 0.377
4.008LeuGln: 4.008 ± 0.469
4.438LeuArg: 4.438 ± 0.602
6.513LeuSer: 6.513 ± 0.718
4.223LeuThr: 4.223 ± 0.588
4.295LeuVal: 4.295 ± 0.395
1.074LeuTrp: 1.074 ± 0.277
2.29LeuTyr: 2.29 ± 0.443
0.0LeuXaa: 0.0 ± 0.0
Met
2.29MetAla: 2.29 ± 0.386
0.072MetCys: 0.072 ± 0.066
1.288MetAsp: 1.288 ± 0.24
1.646MetGlu: 1.646 ± 0.348
1.288MetPhe: 1.288 ± 0.304
1.789MetGly: 1.789 ± 0.359
0.286MetHis: 0.286 ± 0.135
1.432MetIle: 1.432 ± 0.293
2.863MetLys: 2.863 ± 0.567
1.646MetLeu: 1.646 ± 0.306
1.217MetMet: 1.217 ± 0.299
1.503MetAsn: 1.503 ± 0.253
0.644MetPro: 0.644 ± 0.227
1.36MetGln: 1.36 ± 0.303
1.575MetArg: 1.575 ± 0.351
2.076MetSer: 2.076 ± 0.373
1.861MetThr: 1.861 ± 0.382
1.074MetVal: 1.074 ± 0.229
0.072MetTrp: 0.072 ± 0.073
0.716MetTyr: 0.716 ± 0.223
0.0MetXaa: 0.0 ± 0.0
Asn
2.505AsnAla: 2.505 ± 0.67
0.215AsnCys: 0.215 ± 0.128
2.004AsnAsp: 2.004 ± 0.392
3.078AsnGlu: 3.078 ± 0.408
1.718AsnPhe: 1.718 ± 0.298
3.293AsnGly: 3.293 ± 0.492
1.074AsnHis: 1.074 ± 0.285
3.794AsnIle: 3.794 ± 0.539
4.509AsnLys: 4.509 ± 0.607
4.008AsnLeu: 4.008 ± 0.509
1.288AsnMet: 1.288 ± 0.319
3.006AsnAsn: 3.006 ± 0.527
1.575AsnPro: 1.575 ± 0.341
2.076AsnGln: 2.076 ± 0.346
2.577AsnArg: 2.577 ± 0.439
3.006AsnSer: 3.006 ± 0.498
2.863AsnThr: 2.863 ± 0.546
3.221AsnVal: 3.221 ± 0.537
0.93AsnTrp: 0.93 ± 0.234
2.147AsnTyr: 2.147 ± 0.438
0.0AsnXaa: 0.0 ± 0.0
Pro
1.933ProAla: 1.933 ± 0.392
0.286ProCys: 0.286 ± 0.141
2.004ProAsp: 2.004 ± 0.31
2.076ProGlu: 2.076 ± 0.383
1.789ProPhe: 1.789 ± 0.337
1.002ProGly: 1.002 ± 0.275
0.215ProHis: 0.215 ± 0.134
2.29ProIle: 2.29 ± 0.323
2.72ProLys: 2.72 ± 0.527
1.861ProLeu: 1.861 ± 0.316
0.429ProMet: 0.429 ± 0.15
2.076ProAsn: 2.076 ± 0.499
1.074ProPro: 1.074 ± 0.299
1.074ProGln: 1.074 ± 0.303
1.432ProArg: 1.432 ± 0.338
1.646ProSer: 1.646 ± 0.343
2.004ProThr: 2.004 ± 0.439
1.933ProVal: 1.933 ± 0.314
0.716ProTrp: 0.716 ± 0.212
1.217ProTyr: 1.217 ± 0.261
0.0ProXaa: 0.0 ± 0.0
Gln
3.364GlnAla: 3.364 ± 0.551
0.143GlnCys: 0.143 ± 0.091
1.503GlnAsp: 1.503 ± 0.31
2.648GlnGlu: 2.648 ± 0.382
0.93GlnPhe: 0.93 ± 0.263
2.29GlnGly: 2.29 ± 0.389
0.429GlnHis: 0.429 ± 0.161
4.438GlnIle: 4.438 ± 0.549
2.577GlnLys: 2.577 ± 0.41
3.937GlnLeu: 3.937 ± 0.462
1.288GlnMet: 1.288 ± 0.275
2.147GlnAsn: 2.147 ± 0.485
0.93GlnPro: 0.93 ± 0.229
2.076GlnGln: 2.076 ± 0.443
2.147GlnArg: 2.147 ± 0.375
1.789GlnSer: 1.789 ± 0.379
2.362GlnThr: 2.362 ± 0.357
2.791GlnVal: 2.791 ± 0.355
0.286GlnTrp: 0.286 ± 0.123
1.432GlnTyr: 1.432 ± 0.379
0.0GlnXaa: 0.0 ± 0.0
Arg
2.72ArgAla: 2.72 ± 0.44
0.501ArgCys: 0.501 ± 0.216
2.72ArgAsp: 2.72 ± 0.469
4.581ArgGlu: 4.581 ± 0.575
2.219ArgPhe: 2.219 ± 0.412
3.149ArgGly: 3.149 ± 0.418
0.573ArgHis: 0.573 ± 0.298
2.863ArgIle: 2.863 ± 0.434
5.082ArgLys: 5.082 ± 0.715
4.939ArgLeu: 4.939 ± 0.753
1.718ArgMet: 1.718 ± 0.399
1.718ArgAsn: 1.718 ± 0.3
1.074ArgPro: 1.074 ± 0.24
2.076ArgGln: 2.076 ± 0.401
2.505ArgArg: 2.505 ± 0.394
2.076ArgSer: 2.076 ± 0.402
2.434ArgThr: 2.434 ± 0.51
3.579ArgVal: 3.579 ± 0.445
0.93ArgTrp: 0.93 ± 0.234
2.219ArgTyr: 2.219 ± 0.32
0.0ArgXaa: 0.0 ± 0.0
Ser
3.078SerAla: 3.078 ± 0.394
0.215SerCys: 0.215 ± 0.123
3.865SerAsp: 3.865 ± 0.492
4.438SerGlu: 4.438 ± 0.625
3.221SerPhe: 3.221 ± 0.552
3.865SerGly: 3.865 ± 0.347
1.002SerHis: 1.002 ± 0.222
3.865SerIle: 3.865 ± 0.484
4.223SerLys: 4.223 ± 0.469
5.655SerLeu: 5.655 ± 0.611
1.36SerMet: 1.36 ± 0.28
2.72SerAsn: 2.72 ± 0.436
2.362SerPro: 2.362 ± 0.402
1.861SerGln: 1.861 ± 0.294
1.646SerArg: 1.646 ± 0.346
3.865SerSer: 3.865 ± 0.673
3.221SerThr: 3.221 ± 0.484
4.223SerVal: 4.223 ± 0.512
0.573SerTrp: 0.573 ± 0.194
2.505SerTyr: 2.505 ± 0.48
0.0SerXaa: 0.0 ± 0.0
Thr
4.223ThrAla: 4.223 ± 0.661
0.143ThrCys: 0.143 ± 0.1
3.794ThrAsp: 3.794 ± 0.617
4.939ThrGlu: 4.939 ± 0.538
1.718ThrPhe: 1.718 ± 0.367
4.151ThrGly: 4.151 ± 0.568
1.217ThrHis: 1.217 ± 0.241
4.295ThrIle: 4.295 ± 0.593
4.724ThrLys: 4.724 ± 0.721
5.297ThrLeu: 5.297 ± 0.584
1.002ThrMet: 1.002 ± 0.253
2.362ThrAsn: 2.362 ± 0.356
1.646ThrPro: 1.646 ± 0.346
1.432ThrGln: 1.432 ± 0.279
1.933ThrArg: 1.933 ± 0.391
3.149ThrSer: 3.149 ± 0.632
3.579ThrThr: 3.579 ± 0.523
4.724ThrVal: 4.724 ± 0.554
0.787ThrTrp: 0.787 ± 0.239
2.219ThrTyr: 2.219 ± 0.36
0.0ThrXaa: 0.0 ± 0.0
Val
5.082ValAla: 5.082 ± 0.634
0.501ValCys: 0.501 ± 0.218
5.511ValAsp: 5.511 ± 0.479
4.438ValGlu: 4.438 ± 0.561
2.147ValPhe: 2.147 ± 0.504
4.08ValGly: 4.08 ± 0.522
0.93ValHis: 0.93 ± 0.236
5.01ValIle: 5.01 ± 0.532
4.08ValLys: 4.08 ± 0.489
5.154ValLeu: 5.154 ± 0.761
1.861ValMet: 1.861 ± 0.323
3.078ValAsn: 3.078 ± 0.542
2.434ValPro: 2.434 ± 0.372
2.935ValGln: 2.935 ± 0.377
3.293ValArg: 3.293 ± 0.514
3.579ValSer: 3.579 ± 0.511
4.796ValThr: 4.796 ± 0.573
5.726ValVal: 5.726 ± 0.699
0.716ValTrp: 0.716 ± 0.226
3.078ValTyr: 3.078 ± 0.471
0.0ValXaa: 0.0 ± 0.0
Trp
0.429TrpAla: 0.429 ± 0.188
0.143TrpCys: 0.143 ± 0.113
0.286TrpAsp: 0.286 ± 0.143
1.145TrpGlu: 1.145 ± 0.286
0.501TrpPhe: 0.501 ± 0.194
1.074TrpGly: 1.074 ± 0.233
0.215TrpHis: 0.215 ± 0.147
0.93TrpIle: 0.93 ± 0.232
1.646TrpLys: 1.646 ± 0.316
1.217TrpLeu: 1.217 ± 0.24
0.573TrpMet: 0.573 ± 0.235
0.358TrpAsn: 0.358 ± 0.149
0.143TrpPro: 0.143 ± 0.089
0.573TrpGln: 0.573 ± 0.182
0.787TrpArg: 0.787 ± 0.219
0.787TrpSer: 0.787 ± 0.237
0.859TrpThr: 0.859 ± 0.221
1.002TrpVal: 1.002 ± 0.258
0.501TrpTrp: 0.501 ± 0.253
0.143TrpTyr: 0.143 ± 0.099
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.72TyrAla: 2.72 ± 0.358
0.429TyrCys: 0.429 ± 0.176
2.72TyrAsp: 2.72 ± 0.354
3.937TyrGlu: 3.937 ± 0.624
1.718TyrPhe: 1.718 ± 0.306
2.648TyrGly: 2.648 ± 0.45
1.145TyrHis: 1.145 ± 0.306
2.791TyrIle: 2.791 ± 0.46
2.434TyrLys: 2.434 ± 0.438
3.65TyrLeu: 3.65 ± 0.458
0.644TyrMet: 0.644 ± 0.189
2.147TyrAsn: 2.147 ± 0.386
1.145TyrPro: 1.145 ± 0.271
1.145TyrGln: 1.145 ± 0.229
2.219TyrArg: 2.219 ± 0.46
1.503TyrSer: 1.503 ± 0.299
1.933TyrThr: 1.933 ± 0.386
2.72TyrVal: 2.72 ± 0.47
0.358TyrTrp: 0.358 ± 0.169
1.575TyrTyr: 1.575 ± 0.366
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 94 proteins (13972 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski