Hipposideros bat coronavirus HKU10

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae; Alphacoronavirus; Decacovirus; Bat coronavirus HKU10

Average proteome isoelectric point is 7.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K4JZR1|K4JZR1_9ALPC Envelope small membrane protein OS=Hipposideros bat coronavirus HKU10 OX=1241932 GN=E PE=3 SV=1
MM1 pKa = 7.54ISLAVYY7 pKa = 9.88LAEE10 pKa = 4.44LLVLITNLPYY20 pKa = 10.39TRR22 pKa = 11.84ATCSSAQNPAALPNLNLGLPANSNVFVSGYY52 pKa = 10.83LPIEE56 pKa = 4.32NNWRR60 pKa = 11.84CQTGDD65 pKa = 3.18TVYY68 pKa = 11.22NNVNAVYY75 pKa = 9.78WSYY78 pKa = 11.22YY79 pKa = 8.12SHH81 pKa = 6.45GTPVEE86 pKa = 3.99LAISNSDD93 pKa = 3.3QLQNADD99 pKa = 2.79QWALYY104 pKa = 9.59IYY106 pKa = 10.15QGNNYY111 pKa = 9.97GYY113 pKa = 8.51MHH115 pKa = 7.11FKK117 pKa = 8.59ICKK120 pKa = 7.96WNGKK124 pKa = 9.28YY125 pKa = 10.66SFINNPYY132 pKa = 8.81PSRR135 pKa = 11.84GVCMYY140 pKa = 10.3NKK142 pKa = 9.77KK143 pKa = 10.35FPFVFPHH150 pKa = 6.35ARR152 pKa = 11.84NTVIGASWSGDD163 pKa = 3.29YY164 pKa = 9.35VTLFTTTSARR174 pKa = 11.84RR175 pKa = 11.84FYY177 pKa = 11.26LKK179 pKa = 10.91NNWSRR184 pKa = 11.84VNVKK188 pKa = 10.12CQLAEE193 pKa = 3.84SCAIQTVSNINTLNITTNQQGFIQSYY219 pKa = 7.57SVCTEE224 pKa = 3.44CDD226 pKa = 3.29GFPKK230 pKa = 10.36HH231 pKa = 5.93VFPVLDD237 pKa = 4.46DD238 pKa = 4.02GKK240 pKa = 10.78IPGDD244 pKa = 3.37FSFSNWFVLTNSSTVLQGRR263 pKa = 11.84IVTVQPLKK271 pKa = 10.72LLCLWPVPSLNANDD285 pKa = 3.57KK286 pKa = 10.18PVYY289 pKa = 10.41FNLSLNSDD297 pKa = 3.68VRR299 pKa = 11.84CNGHH303 pKa = 6.53SIAGYY308 pKa = 9.05AQALRR313 pKa = 11.84FSLNFTANQVLNGVHH328 pKa = 6.46SIKK331 pKa = 10.63LFSSNQNFTFTCSTNGTLEE350 pKa = 4.59RR351 pKa = 11.84IPFGRR356 pKa = 11.84VEE358 pKa = 4.17SVYY361 pKa = 10.92KK362 pKa = 10.43CFVRR366 pKa = 11.84IDD368 pKa = 4.58AINSTKK374 pKa = 10.84NLFVGILPPVTKK386 pKa = 10.09EE387 pKa = 3.93FVISRR392 pKa = 11.84YY393 pKa = 8.85GAVYY397 pKa = 10.29INGVHH402 pKa = 6.55IMSLPPLEE410 pKa = 4.47SVIFNVTSSVGSDD423 pKa = 2.86FWTVAFADD431 pKa = 4.03EE432 pKa = 5.14AEE434 pKa = 4.28VLLDD438 pKa = 4.27LNATNIVDD446 pKa = 4.24ILYY449 pKa = 10.61CDD451 pKa = 3.59TLVNKK456 pKa = 10.05IKK458 pKa = 10.47CQNLAFSLEE467 pKa = 4.2DD468 pKa = 3.35GFYY471 pKa = 10.03ATSNLIEE478 pKa = 4.37RR479 pKa = 11.84DD480 pKa = 3.2IPRR483 pKa = 11.84SFITLPYY490 pKa = 9.48HH491 pKa = 4.73VTHH494 pKa = 6.33SVVGLDD500 pKa = 3.2VRR502 pKa = 11.84IYY504 pKa = 10.6YY505 pKa = 10.39EE506 pKa = 4.48NEE508 pKa = 3.48AASNLTLSPSDD519 pKa = 3.85PVCVDD524 pKa = 3.35SSQFSTYY531 pKa = 10.62FNVTADD537 pKa = 3.67TNGFNARR544 pKa = 11.84IINVDD549 pKa = 3.6CPFTFDD555 pKa = 4.45KK556 pKa = 10.85LNNYY560 pKa = 9.53ISFEE564 pKa = 4.42SICFSLEE571 pKa = 3.75PFAGGCSMRR580 pKa = 11.84IEE582 pKa = 4.73SFFSSYY588 pKa = 9.75VRR590 pKa = 11.84TVGSLYY596 pKa = 10.42ILFKK600 pKa = 10.84NGNKK604 pKa = 7.98ITGVTEE610 pKa = 4.26SSAGVYY616 pKa = 7.67DD617 pKa = 3.7TSFLHH622 pKa = 7.0TDD624 pKa = 2.39ICTDD628 pKa = 3.19YY629 pKa = 10.6TIYY632 pKa = 9.58GTTGRR637 pKa = 11.84GVITKK642 pKa = 10.54LNTTIIAGLYY652 pKa = 6.91YY653 pKa = 10.2TSQSGQILGFKK664 pKa = 10.15NATNGDD670 pKa = 3.56IYY672 pKa = 11.23AVRR675 pKa = 11.84PCDD678 pKa = 3.88LSSQAAVVSNQIVAVMSASEE698 pKa = 4.28NITFGFNNSLEE709 pKa = 4.12LPKK712 pKa = 10.48FYY714 pKa = 10.72LHH716 pKa = 7.09SNAEE720 pKa = 4.0NATNCTEE727 pKa = 3.78PVLIYY732 pKa = 10.46GKK734 pKa = 10.23IGICEE739 pKa = 4.43DD740 pKa = 3.64GSITEE745 pKa = 4.03VKK747 pKa = 10.08PRR749 pKa = 11.84IAEE752 pKa = 4.02PEE754 pKa = 4.27PYY756 pKa = 10.41SPIVTANITIPSNFSISVQAEE777 pKa = 4.07YY778 pKa = 11.36VQMFLTPVSVDD789 pKa = 2.89CNMYY793 pKa = 8.67VCNGNRR799 pKa = 11.84HH800 pKa = 5.67CLRR803 pKa = 11.84LLGEE807 pKa = 4.35YY808 pKa = 8.5VTACKK813 pKa = 10.52NIEE816 pKa = 3.94AALQLSARR824 pKa = 11.84LEE826 pKa = 4.39SIEE829 pKa = 4.0VNNFITVSEE838 pKa = 4.18NAISLANISNFDD850 pKa = 3.75SYY852 pKa = 11.7NLSVLLPKK860 pKa = 10.6QNGKK864 pKa = 9.43SVIEE868 pKa = 5.22DD869 pKa = 3.19ILFDD873 pKa = 3.77KK874 pKa = 11.17VVTNGLGTVDD884 pKa = 3.88QDD886 pKa = 3.64YY887 pKa = 10.93KK888 pKa = 11.0EE889 pKa = 4.41CIKK892 pKa = 10.77RR893 pKa = 11.84SGVKK897 pKa = 10.13DD898 pKa = 3.69VADD901 pKa = 3.83VACAQYY907 pKa = 11.33YY908 pKa = 9.05NGIMVLPGVVDD919 pKa = 3.87DD920 pKa = 5.03VKK922 pKa = 10.74MGLYY926 pKa = 8.32TASLTGAMVMGGLTAAAAIPFSIAVQSRR954 pKa = 11.84LNYY957 pKa = 9.76VALQTDD963 pKa = 4.37VLQKK967 pKa = 10.4NQQILADD974 pKa = 3.9SFNNAMSNITMAFAQVNDD992 pKa = 4.76AIKK995 pKa = 9.04EE996 pKa = 4.23TSQAISTVAQALSKK1010 pKa = 9.8VQSVVNEE1017 pKa = 3.85QGEE1020 pKa = 4.4ALSQLTKK1027 pKa = 10.68QLASNFQAISSSIEE1041 pKa = 4.09DD1042 pKa = 3.27IYY1044 pKa = 11.77NRR1046 pKa = 11.84LDD1048 pKa = 3.58MIAADD1053 pKa = 3.79AQVDD1057 pKa = 3.76RR1058 pKa = 11.84LITGRR1063 pKa = 11.84IGALNAFVTQTLTKK1077 pKa = 8.05YY1078 pKa = 9.0TEE1080 pKa = 4.13VRR1082 pKa = 11.84ASRR1085 pKa = 11.84QLALQKK1091 pKa = 10.44INEE1094 pKa = 4.49CVKK1097 pKa = 10.17SQSSRR1102 pKa = 11.84FGFCGNGTHH1111 pKa = 7.24LFSIANAAPNGIMLFHH1127 pKa = 6.46TVLMPTEE1134 pKa = 4.21YY1135 pKa = 10.84VVVRR1139 pKa = 11.84AWAGLCIEE1147 pKa = 5.14DD1148 pKa = 3.77KK1149 pKa = 11.3AFVLRR1154 pKa = 11.84DD1155 pKa = 3.42VKK1157 pKa = 9.04TTLFKK1162 pKa = 10.76TGDD1165 pKa = 3.21TYY1167 pKa = 11.66YY1168 pKa = 9.45ITTRR1172 pKa = 11.84DD1173 pKa = 3.53MYY1175 pKa = 10.55QPRR1178 pKa = 11.84LPQVSDD1184 pKa = 3.59FVQIKK1189 pKa = 9.92DD1190 pKa = 3.85CQVDD1194 pKa = 3.89YY1195 pKa = 11.75LNLTSDD1201 pKa = 3.74EE1202 pKa = 4.43VNNVIPDD1209 pKa = 3.89YY1210 pKa = 11.16IDD1212 pKa = 3.32VNKK1215 pKa = 9.54TLEE1218 pKa = 4.33DD1219 pKa = 3.97FGQILANNTRR1229 pKa = 11.84PPIPDD1234 pKa = 3.71FGLDD1238 pKa = 3.41VYY1240 pKa = 11.78NNTILNLTAEE1250 pKa = 4.39IDD1252 pKa = 3.73VLQNKK1257 pKa = 9.45SEE1259 pKa = 4.0QLLASTEE1266 pKa = 4.02RR1267 pKa = 11.84LQQLIDD1273 pKa = 4.05NLNKK1277 pKa = 10.23TYY1279 pKa = 11.22VDD1281 pKa = 4.26LEE1283 pKa = 3.94WLNRR1287 pKa = 11.84FEE1289 pKa = 6.35QYY1291 pKa = 10.44IKK1293 pKa = 9.88WPWYY1297 pKa = 7.76VWLAIFLAVILFSFLMLYY1315 pKa = 9.17CCCATGCCGCFSCLSNSCDD1334 pKa = 3.54CRR1336 pKa = 11.84GKK1338 pKa = 9.87NLQRR1342 pKa = 11.84YY1343 pKa = 6.8EE1344 pKa = 4.04VEE1346 pKa = 4.22KK1347 pKa = 11.1VHH1349 pKa = 6.52IQQ1351 pKa = 3.09

Molecular weight: 149.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K4K1W8|K4K1W8_9ALPC Non-structural protein NS7b OS=Hipposideros bat coronavirus HKU10 OX=1241932 GN=NS7b PE=4 SV=1
MM1 pKa = 7.72ASGGNNVSFANKK13 pKa = 8.8PRR15 pKa = 11.84GRR17 pKa = 11.84SGRR20 pKa = 11.84VPLSYY25 pKa = 10.44YY26 pKa = 10.07SPVMIDD32 pKa = 3.05GDD34 pKa = 3.97QPFWKK39 pKa = 10.36VMPTNAVPTGMGDD52 pKa = 3.06KK53 pKa = 10.15NQQIGYY59 pKa = 9.11WNEE62 pKa = 3.52QPRR65 pKa = 11.84WRR67 pKa = 11.84MVKK70 pKa = 9.7GQRR73 pKa = 11.84KK74 pKa = 7.82EE75 pKa = 4.33LQSKK79 pKa = 6.13WHH81 pKa = 6.6FYY83 pKa = 11.09YY84 pKa = 10.85LGTGPQEE91 pKa = 4.08DD92 pKa = 3.12ARR94 pKa = 11.84YY95 pKa = 7.83RR96 pKa = 11.84QRR98 pKa = 11.84IEE100 pKa = 3.74GVFWVAVQGSKK111 pKa = 9.59TDD113 pKa = 3.49PTGLGTRR120 pKa = 11.84RR121 pKa = 11.84KK122 pKa = 8.71GQNLISPKK130 pKa = 9.89FAVKK134 pKa = 10.13IPSNVVVVEE143 pKa = 4.29EE144 pKa = 4.29TSRR147 pKa = 11.84PPSRR151 pKa = 11.84SQSNSRR157 pKa = 11.84PQSRR161 pKa = 11.84NNSRR165 pKa = 11.84PQSKK169 pKa = 10.58NNSQNNSRR177 pKa = 11.84DD178 pKa = 3.29NSRR181 pKa = 11.84APSRR185 pKa = 11.84PRR187 pKa = 11.84SRR189 pKa = 11.84ANSTSSSANDD199 pKa = 3.39AVDD202 pKa = 3.31IVAAVKK208 pKa = 10.1QALKK212 pKa = 10.26EE213 pKa = 4.02LGVTPEE219 pKa = 4.56KK220 pKa = 10.79KK221 pKa = 10.09NQEE224 pKa = 4.04KK225 pKa = 10.12QKK227 pKa = 10.76KK228 pKa = 8.81SNSGSNTPKK237 pKa = 10.02EE238 pKa = 4.25AATSRR243 pKa = 11.84QKK245 pKa = 10.91SSPSTPRR252 pKa = 11.84KK253 pKa = 8.69QLEE256 pKa = 3.86RR257 pKa = 11.84PEE259 pKa = 4.19WKK261 pKa = 9.81RR262 pKa = 11.84VPNKK266 pKa = 9.77EE267 pKa = 4.14EE268 pKa = 3.79NVIACFGPRR277 pKa = 11.84DD278 pKa = 3.77TLHH281 pKa = 6.7NFGDD285 pKa = 3.51QKK287 pKa = 11.22LIAEE291 pKa = 5.32GVQAPHH297 pKa = 6.22YY298 pKa = 7.82PQLAEE303 pKa = 4.7LVPTPAALLFGGEE316 pKa = 3.86VSTRR320 pKa = 11.84EE321 pKa = 3.78RR322 pKa = 11.84GDD324 pKa = 3.41EE325 pKa = 4.01VEE327 pKa = 4.28IMYY330 pKa = 9.8VYY332 pKa = 10.7KK333 pKa = 10.53MKK335 pKa = 10.38VHH337 pKa = 7.05KK338 pKa = 10.43SNKK341 pKa = 9.17DD342 pKa = 3.3LPSFLQQVSAYY353 pKa = 9.58AQAPDD358 pKa = 3.13ATTTEE363 pKa = 4.34KK364 pKa = 10.66ATVQPLLNPTAPDD377 pKa = 4.32FKK379 pKa = 10.45PATSDD384 pKa = 3.57EE385 pKa = 4.53VIEE388 pKa = 4.78MINTVYY394 pKa = 11.1DD395 pKa = 3.54SFDD398 pKa = 3.03AA399 pKa = 4.89

Molecular weight: 44.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

0

8

9279

75

6781

1159.9

129.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.466 ± 0.254

3.222 ± 0.721

5.151 ± 0.575

4.171 ± 0.233

5.582 ± 0.432

6.1 ± 0.585

1.875 ± 0.286

5.216 ± 0.559

5.787 ± 0.848

8.816 ± 0.567

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.145 ± 0.249

5.884 ± 0.766

3.675 ± 0.691

3.567 ± 0.565

3.449 ± 0.51

7.167 ± 0.616

6.24 ± 0.405

9.613 ± 0.493

1.25 ± 0.27

4.623 ± 0.279
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski