Microbispora rosea

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptosporangiales; Streptosporangiaceae; Microbispora

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8224 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1N6R5A4|A0A1N6R5A4_9ACTN 1-acyl-sn-glycerol-3-phosphate acyltransferase OS=Microbispora rosea OX=58117 GN=SAMN05421833_101243 PE=4 SV=1
MM1 pKa = 6.71QTTDD5 pKa = 4.6LAKK8 pKa = 10.11AWKK11 pKa = 10.19DD12 pKa = 3.27PGFSATLTAEE22 pKa = 4.22QLSTLPANPVGDD34 pKa = 4.19LADD37 pKa = 3.54IADD40 pKa = 4.26FANEE44 pKa = 4.49GEE46 pKa = 4.45VQPIMMSWAPCWITWSSDD64 pKa = 3.64TNCCAYY70 pKa = 10.5

Molecular weight: 7.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1N7GAW6|A0A1N7GAW6_9ACTN Gas vesicle synthesis protein GvpO OS=Microbispora rosea OX=58117 GN=SAMN05421833_12742 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.99KK16 pKa = 9.77HH17 pKa = 5.81RR18 pKa = 11.84KK19 pKa = 8.55LLKK22 pKa = 8.44KK23 pKa = 9.17TRR25 pKa = 11.84IQRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.73KK32 pKa = 9.84

Molecular weight: 4.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8224

0

8224

2616702

26

3311

318.2

34.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.537 ± 0.045

0.796 ± 0.008

5.808 ± 0.024

5.57 ± 0.027

2.775 ± 0.016

9.542 ± 0.024

2.17 ± 0.015

3.432 ± 0.017

1.91 ± 0.02

10.391 ± 0.034

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.857 ± 0.008

1.794 ± 0.017

6.133 ± 0.025

2.566 ± 0.015

8.387 ± 0.036

5.032 ± 0.024

5.84 ± 0.023

8.774 ± 0.027

1.564 ± 0.014

2.121 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski