Amino acid dipepetide frequency for Gammaproteobacteria bacterium ESL0073

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.265AlaAla: 6.265 ± 0.091	0.963AlaCys: 0.963 ± 0.035	4.094AlaAsp: 4.094 ± 0.072	4.607AlaGlu: 4.607 ± 0.09	3.096AlaPhe: 3.096 ± 0.065	5.359AlaGly: 5.359 ± 0.095	1.494AlaHis: 1.494 ± 0.048	6.537AlaIle: 6.537 ± 0.1	5.226AlaLys: 5.226 ± 0.089	8.769AlaLeu: 8.769 ± 0.116	2.119AlaMet: 2.119 ± 0.05	3.857AlaAsn: 3.857 ± 0.079	2.673AlaPro: 2.673 ± 0.076	3.368AlaGln: 3.368 ± 0.069	3.257AlaArg: 3.257 ± 0.064	4.755AlaSer: 4.755 ± 0.078	4.506AlaThr: 4.506 ± 0.082	5.09AlaVal: 5.09 ± 0.095	1.006AlaTrp: 1.006 ± 0.039	2.492AlaTyr: 2.492 ± 0.064	0.0AlaXaa: 0.0 ± 0.0
Cys	0.799CysAla: 0.799 ± 0.027	0.213CysCys: 0.213 ± 0.019	0.577CysAsp: 0.577 ± 0.029	0.547CysGlu: 0.547 ± 0.024	0.558CysPhe: 0.558 ± 0.026	0.899CysGly: 0.899 ± 0.031	0.319CysHis: 0.319 ± 0.02	0.793CysIle: 0.793 ± 0.026	0.53CysLys: 0.53 ± 0.023	1.198CysLeu: 1.198 ± 0.042	0.23CysMet: 0.23 ± 0.015	0.439CysAsn: 0.439 ± 0.02	0.503CysPro: 0.503 ± 0.028	0.493CysGln: 0.493 ± 0.024	0.411CysArg: 0.411 ± 0.023	0.742CysSer: 0.742 ± 0.03	0.535CysThr: 0.535 ± 0.022	0.677CysVal: 0.677 ± 0.028	0.132CysTrp: 0.132 ± 0.01	0.467CysTyr: 0.467 ± 0.022	0.0CysXaa: 0.0 ± 0.0
Asp	3.678AspAla: 3.678 ± 0.073	0.546AspCys: 0.546 ± 0.029	2.653AspAsp: 2.653 ± 0.061	3.371AspGlu: 3.371 ± 0.071	2.342AspPhe: 2.342 ± 0.056	3.365AspGly: 3.365 ± 0.082	1.075AspHis: 1.075 ± 0.032	4.012AspIle: 4.012 ± 0.067	3.529AspLys: 3.529 ± 0.066	5.193AspLeu: 5.193 ± 0.082	1.143AspMet: 1.143 ± 0.035	2.794AspAsn: 2.794 ± 0.065	1.97AspPro: 1.97 ± 0.052	2.096AspGln: 2.096 ± 0.05	2.201AspArg: 2.201 ± 0.054	2.874AspSer: 2.874 ± 0.062	2.867AspThr: 2.867 ± 0.057	3.26AspVal: 3.26 ± 0.064	0.79AspTrp: 0.79 ± 0.033	2.276AspTyr: 2.276 ± 0.052	0.0AspXaa: 0.0 ± 0.0
Glu	4.872GluAla: 4.872 ± 0.086	0.449GluCys: 0.449 ± 0.022	2.569GluAsp: 2.569 ± 0.055	3.496GluGlu: 3.496 ± 0.091	1.826GluPhe: 1.826 ± 0.048	3.623GluGly: 3.623 ± 0.077	1.336GluHis: 1.336 ± 0.041	3.846GluIle: 3.846 ± 0.073	3.981GluLys: 3.981 ± 0.081	6.236GluLeu: 6.236 ± 0.112	1.347GluMet: 1.347 ± 0.046	2.652GluAsn: 2.652 ± 0.063	1.874GluPro: 1.874 ± 0.049	3.742GluGln: 3.742 ± 0.086	2.988GluArg: 2.988 ± 0.068	3.044GluSer: 3.044 ± 0.064	3.163GluThr: 3.163 ± 0.062	3.804GluVal: 3.804 ± 0.08	0.629GluTrp: 0.629 ± 0.029	1.703GluTyr: 1.703 ± 0.054	0.0GluXaa: 0.0 ± 0.0
Phe	3.034PheAla: 3.034 ± 0.076	0.601PheCys: 0.601 ± 0.029	2.522PheAsp: 2.522 ± 0.052	2.07PheGlu: 2.07 ± 0.048	2.021PhePhe: 2.021 ± 0.067	2.731PheGly: 2.731 ± 0.075	0.715PheHis: 0.715 ± 0.03	3.527PheIle: 3.527 ± 0.085	2.362PheLys: 2.362 ± 0.058	3.842PheLeu: 3.842 ± 0.075	0.973PheMet: 0.973 ± 0.033	2.234PheAsn: 2.234 ± 0.055	1.412PhePro: 1.412 ± 0.036	1.185PheGln: 1.185 ± 0.033	1.437PheArg: 1.437 ± 0.041	3.134PheSer: 3.134 ± 0.067	2.498PheThr: 2.498 ± 0.059	2.52PheVal: 2.52 ± 0.058	0.563PheTrp: 0.563 ± 0.031	1.611PheTyr: 1.611 ± 0.047	0.0PheXaa: 0.0 ± 0.0
Gly	4.961GlyAla: 4.961 ± 0.094	0.945GlyCys: 0.945 ± 0.034	3.311GlyAsp: 3.311 ± 0.074	3.505GlyGlu: 3.505 ± 0.066	3.134GlyPhe: 3.134 ± 0.062	4.687GlyGly: 4.687 ± 0.095	1.418GlyHis: 1.418 ± 0.044	5.303GlyIle: 5.303 ± 0.088	4.28GlyLys: 4.28 ± 0.074	6.84GlyLeu: 6.84 ± 0.107	1.671GlyMet: 1.671 ± 0.043	3.133GlyAsn: 3.133 ± 0.09	1.425GlyPro: 1.425 ± 0.046	2.669GlyGln: 2.669 ± 0.058	2.841GlyArg: 2.841 ± 0.063	4.154GlySer: 4.154 ± 0.105	3.585GlyThr: 3.585 ± 0.088	4.91GlyVal: 4.91 ± 0.086	0.929GlyTrp: 0.929 ± 0.032	2.77GlyTyr: 2.77 ± 0.07	0.0GlyXaa: 0.0 ± 0.0
His	1.425HisAla: 1.425 ± 0.042	0.329HisCys: 0.329 ± 0.019	1.025HisAsp: 1.025 ± 0.037	0.987HisGlu: 0.987 ± 0.038	1.032HisPhe: 1.032 ± 0.035	1.329HisGly: 1.329 ± 0.043	0.635HisHis: 0.635 ± 0.031	1.487HisIle: 1.487 ± 0.041	1.042HisLys: 1.042 ± 0.035	2.26HisLeu: 2.26 ± 0.051	0.415HisMet: 0.415 ± 0.021	0.969HisAsn: 0.969 ± 0.033	1.088HisPro: 1.088 ± 0.033	0.965HisGln: 0.965 ± 0.035	0.844HisArg: 0.844 ± 0.034	1.178HisSer: 1.178 ± 0.042	1.035HisThr: 1.035 ± 0.034	1.219HisVal: 1.219 ± 0.043	0.341HisTrp: 0.341 ± 0.02	1.02HisTyr: 1.02 ± 0.035	0.0HisXaa: 0.0 ± 0.0
Ile	6.543IleAla: 6.543 ± 0.094	0.838IleCys: 0.838 ± 0.031	4.526IleAsp: 4.526 ± 0.08	4.752IleGlu: 4.752 ± 0.082	2.697IlePhe: 2.697 ± 0.074	5.092IleGly: 5.092 ± 0.087	1.308IleHis: 1.308 ± 0.047	5.539IleIle: 5.539 ± 0.098	4.63IleLys: 4.63 ± 0.08	6.395IleLeu: 6.395 ± 0.106	1.443IleMet: 1.443 ± 0.044	4.163IleAsn: 4.163 ± 0.074	3.338IlePro: 3.338 ± 0.067	2.532IleGln: 2.532 ± 0.05	2.896IleArg: 2.896 ± 0.064	4.78IleSer: 4.78 ± 0.082	4.813IleThr: 4.813 ± 0.081	4.539IleVal: 4.539 ± 0.083	0.703IleTrp: 0.703 ± 0.029	2.233IleTyr: 2.233 ± 0.043	0.0IleXaa: 0.0 ± 0.0
Lys	5.19LysAla: 5.19 ± 0.083	0.363LysCys: 0.363 ± 0.02	3.276LysAsp: 3.276 ± 0.073	4.391LysGlu: 4.391 ± 0.078	1.585LysPhe: 1.585 ± 0.041	3.695LysGly: 3.695 ± 0.06	1.238LysHis: 1.238 ± 0.037	3.87LysIle: 3.87 ± 0.069	4.096LysLys: 4.096 ± 0.09	6.07LysLeu: 6.07 ± 0.092	1.523LysMet: 1.523 ± 0.044	3.077LysAsn: 3.077 ± 0.057	2.577LysPro: 2.577 ± 0.051	3.653LysGln: 3.653 ± 0.078	2.843LysArg: 2.843 ± 0.065	3.204LysSer: 3.204 ± 0.06	3.706LysThr: 3.706 ± 0.069	3.947LysVal: 3.947 ± 0.068	0.552LysTrp: 0.552 ± 0.03	1.716LysTyr: 1.716 ± 0.05	0.0LysXaa: 0.0 ± 0.0
Leu	9.426LeuAla: 9.426 ± 0.114	1.188LeuCys: 1.188 ± 0.038	5.51LeuAsp: 5.51 ± 0.082	5.794LeuGlu: 5.794 ± 0.094	4.545LeuPhe: 4.545 ± 0.098	6.973LeuGly: 6.973 ± 0.106	1.85LeuHis: 1.85 ± 0.046	7.394LeuIle: 7.394 ± 0.107	6.083LeuLys: 6.083 ± 0.087	11.619LeuLeu: 11.619 ± 0.178	2.393LeuMet: 2.393 ± 0.054	5.041LeuAsn: 5.041 ± 0.081	4.962LeuPro: 4.962 ± 0.084	4.086LeuGln: 4.086 ± 0.084	4.253LeuArg: 4.253 ± 0.094	7.726LeuSer: 7.726 ± 0.107	6.4LeuThr: 6.4 ± 0.089	6.49LeuVal: 6.49 ± 0.092	1.157LeuTrp: 1.157 ± 0.039	2.977LeuTyr: 2.977 ± 0.063	0.0LeuXaa: 0.0 ± 0.0
Met	2.118MetAla: 2.118 ± 0.05	0.172MetCys: 0.172 ± 0.014	1.094MetAsp: 1.094 ± 0.032	1.042MetGlu: 1.042 ± 0.037	0.759MetPhe: 0.759 ± 0.034	1.731MetGly: 1.731 ± 0.047	0.483MetHis: 0.483 ± 0.024	1.676MetIle: 1.676 ± 0.053	1.29MetLys: 1.29 ± 0.04	2.429MetLeu: 2.429 ± 0.054	0.639MetMet: 0.639 ± 0.027	0.998MetAsn: 0.998 ± 0.038	1.074MetPro: 1.074 ± 0.038	1.057MetGln: 1.057 ± 0.032	1.0MetArg: 1.0 ± 0.032	1.705MetSer: 1.705 ± 0.047	1.522MetThr: 1.522 ± 0.04	1.585MetVal: 1.585 ± 0.044	0.151MetTrp: 0.151 ± 0.013	0.492MetTyr: 0.492 ± 0.021	0.0MetXaa: 0.0 ± 0.0
Asn	3.622AsnAla: 3.622 ± 0.069	0.462AsnCys: 0.462 ± 0.023	2.464AsnAsp: 2.464 ± 0.059	2.603AsnGlu: 2.603 ± 0.052	1.77AsnPhe: 1.77 ± 0.046	3.26AsnGly: 3.26 ± 0.104	1.087AsnHis: 1.087 ± 0.034	3.806AsnIle: 3.806 ± 0.069	3.337AsnLys: 3.337 ± 0.075	4.467AsnLeu: 4.467 ± 0.072	0.998AsnMet: 0.998 ± 0.034	3.227AsnAsn: 3.227 ± 0.088	2.34AsnPro: 2.34 ± 0.055	2.493AsnGln: 2.493 ± 0.057	1.926AsnArg: 1.926 ± 0.049	2.852AsnSer: 2.852 ± 0.077	3.249AsnThr: 3.249 ± 0.077	2.667AsnVal: 2.667 ± 0.059	0.65AsnTrp: 0.65 ± 0.027	1.931AsnTyr: 1.931 ± 0.055	0.0AsnXaa: 0.0 ± 0.0
Pro	2.803ProAla: 2.803 ± 0.058	0.435ProCys: 0.435 ± 0.024	2.367ProAsp: 2.367 ± 0.057	2.977ProGlu: 2.977 ± 0.057	1.814ProPhe: 1.814 ± 0.051	1.779ProGly: 1.779 ± 0.055	0.814ProHis: 0.814 ± 0.029	3.017ProIle: 3.017 ± 0.061	2.358ProLys: 2.358 ± 0.047	4.216ProLeu: 4.216 ± 0.073	0.99ProMet: 0.99 ± 0.037	2.032ProAsn: 2.032 ± 0.047	1.32ProPro: 1.32 ± 0.047	1.551ProGln: 1.551 ± 0.043	1.384ProArg: 1.384 ± 0.038	2.499ProSer: 2.499 ± 0.048	2.458ProThr: 2.458 ± 0.051	2.859ProVal: 2.859 ± 0.059	0.535ProTrp: 0.535 ± 0.027	1.438ProTyr: 1.438 ± 0.045	0.0ProXaa: 0.0 ± 0.0
Gln	4.088GlnAla: 4.088 ± 0.08	0.415GlnCys: 0.415 ± 0.019	1.817GlnAsp: 1.817 ± 0.044	2.611GlnGlu: 2.611 ± 0.058	1.508GlnPhe: 1.508 ± 0.039	2.983GlnGly: 2.983 ± 0.069	1.062GlnHis: 1.062 ± 0.039	2.761GlnIle: 2.761 ± 0.053	2.566GlnLys: 2.566 ± 0.054	5.424GlnLeu: 5.424 ± 0.104	1.01GlnMet: 1.01 ± 0.038	1.796GlnAsn: 1.796 ± 0.039	1.762GlnPro: 1.762 ± 0.048	3.317GlnGln: 3.317 ± 0.09	2.03GlnArg: 2.03 ± 0.057	2.467GlnSer: 2.467 ± 0.047	2.549GlnThr: 2.549 ± 0.058	3.041GlnVal: 3.041 ± 0.065	0.631GlnTrp: 0.631 ± 0.028	1.65GlnTyr: 1.65 ± 0.049	0.0GlnXaa: 0.0 ± 0.0
Arg	3.044ArgAla: 3.044 ± 0.068	0.473ArgCys: 0.473 ± 0.024	1.993ArgAsp: 1.993 ± 0.057	2.452ArgGlu: 2.452 ± 0.067	2.081ArgPhe: 2.081 ± 0.045	2.555ArgGly: 2.555 ± 0.059	0.938ArgHis: 0.938 ± 0.037	2.943ArgIle: 2.943 ± 0.056	2.393ArgLys: 2.393 ± 0.055	4.999ArgLeu: 4.999 ± 0.082	1.009ArgMet: 1.009 ± 0.033	1.802ArgAsn: 1.802 ± 0.053	1.539ArgPro: 1.539 ± 0.045	2.046ArgGln: 2.046 ± 0.057	1.998ArgArg: 1.998 ± 0.062	2.329ArgSer: 2.329 ± 0.049	1.999ArgThr: 1.999 ± 0.048	2.816ArgVal: 2.816 ± 0.06	0.571ArgTrp: 0.571 ± 0.025	1.875ArgTyr: 1.875 ± 0.054	0.0ArgXaa: 0.0 ± 0.0
Ser	4.613SerAla: 4.613 ± 0.077	0.683SerCys: 0.683 ± 0.029	3.088SerAsp: 3.088 ± 0.068	3.139SerGlu: 3.139 ± 0.063	3.048SerPhe: 3.048 ± 0.069	4.412SerGly: 4.412 ± 0.085	1.4SerHis: 1.4 ± 0.036	4.645SerIle: 4.645 ± 0.084	3.351SerLys: 3.351 ± 0.06	6.978SerLeu: 6.978 ± 0.106	1.5SerMet: 1.5 ± 0.044	2.999SerAsn: 2.999 ± 0.084	2.361SerPro: 2.361 ± 0.054	2.608SerGln: 2.608 ± 0.056	2.448SerArg: 2.448 ± 0.058	4.386SerSer: 4.386 ± 0.112	3.707SerThr: 3.707 ± 0.078	3.985SerVal: 3.985 ± 0.072	0.764SerTrp: 0.764 ± 0.028	2.315SerTyr: 2.315 ± 0.061	0.0SerXaa: 0.0 ± 0.0
Thr	4.525ThrAla: 4.525 ± 0.078	0.6ThrCys: 0.6 ± 0.029	3.084ThrAsp: 3.084 ± 0.069	3.056ThrGlu: 3.056 ± 0.071	2.113ThrPhe: 2.113 ± 0.051	4.358ThrGly: 4.358 ± 0.102	1.255ThrHis: 1.255 ± 0.034	4.36ThrIle: 4.36 ± 0.078	3.167ThrLys: 3.167 ± 0.068	6.808ThrLeu: 6.808 ± 0.101	1.042ThrMet: 1.042 ± 0.035	2.692ThrAsn: 2.692 ± 0.068	3.118ThrPro: 3.118 ± 0.056	2.534ThrGln: 2.534 ± 0.059	2.187ThrArg: 2.187 ± 0.051	3.361ThrSer: 3.361 ± 0.062	3.701ThrThr: 3.701 ± 0.082	3.942ThrVal: 3.942 ± 0.067	0.74ThrTrp: 0.74 ± 0.031	1.905ThrTyr: 1.905 ± 0.059	0.0ThrXaa: 0.0 ± 0.0
Val	5.266ValAla: 5.266 ± 0.091	0.744ValCys: 0.744 ± 0.034	3.617ValAsp: 3.617 ± 0.067	3.673ValGlu: 3.673 ± 0.065	2.855ValPhe: 2.855 ± 0.068	4.515ValGly: 4.515 ± 0.084	1.029ValHis: 1.029 ± 0.035	5.19ValIle: 5.19 ± 0.085	3.893ValLys: 3.893 ± 0.065	6.569ValLeu: 6.569 ± 0.095	1.614ValMet: 1.614 ± 0.044	3.139ValAsn: 3.139 ± 0.067	2.375ValPro: 2.375 ± 0.047	1.994ValGln: 1.994 ± 0.049	2.603ValArg: 2.603 ± 0.057	4.36ValSer: 4.36 ± 0.066	3.869ValThr: 3.869 ± 0.071	4.769ValVal: 4.769 ± 0.084	0.739ValTrp: 0.739 ± 0.032	1.924ValTyr: 1.924 ± 0.055	0.0ValXaa: 0.0 ± 0.0
Trp	0.747TrpAla: 0.747 ± 0.029	0.168TrpCys: 0.168 ± 0.014	0.556TrpAsp: 0.556 ± 0.028	0.492TrpGlu: 0.492 ± 0.025	0.617TrpPhe: 0.617 ± 0.03	0.795TrpGly: 0.795 ± 0.041	0.327TrpHis: 0.327 ± 0.019	0.798TrpIle: 0.798 ± 0.032	0.591TrpLys: 0.591 ± 0.025	1.758TrpLeu: 1.758 ± 0.053	0.303TrpMet: 0.303 ± 0.019	0.476TrpAsn: 0.476 ± 0.021	0.413TrpPro: 0.413 ± 0.023	0.967TrpGln: 0.967 ± 0.037	0.649TrpArg: 0.649 ± 0.028	0.742TrpSer: 0.742 ± 0.028	0.508TrpThr: 0.508 ± 0.023	0.757TrpVal: 0.757 ± 0.026	0.18TrpTrp: 0.18 ± 0.015	0.472TrpTyr: 0.472 ± 0.027	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.51TyrAla: 2.51 ± 0.058	0.478TyrCys: 0.478 ± 0.027	1.891TyrAsp: 1.891 ± 0.062	1.614TyrGlu: 1.614 ± 0.046	1.592TyrPhe: 1.592 ± 0.049	2.333TyrGly: 2.333 ± 0.057	0.847TyrHis: 0.847 ± 0.031	2.254TyrIle: 2.254 ± 0.056	1.867TyrLys: 1.867 ± 0.046	3.799TyrLeu: 3.799 ± 0.089	0.654TyrMet: 0.654 ± 0.027	1.692TyrAsn: 1.692 ± 0.054	1.52TyrPro: 1.52 ± 0.046	2.132TyrGln: 2.132 ± 0.064	1.659TyrArg: 1.659 ± 0.04	2.134TyrSer: 2.134 ± 0.051	1.88TyrThr: 1.88 ± 0.057	1.859TyrVal: 1.859 ± 0.045	0.554TyrTrp: 0.554 ± 0.025	1.383TyrTyr: 1.383 ± 0.047	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2886 proteins (911864 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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