Amino acid dipepetide frequency for Anisakis simplex (Herring worm)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.167AlaAla: 6.167 ± 0.059	1.192AlaCys: 1.192 ± 0.016	3.694AlaAsp: 3.694 ± 0.027	4.357AlaGlu: 4.357 ± 0.036	2.816AlaPhe: 2.816 ± 0.025	3.48AlaGly: 3.48 ± 0.036	1.611AlaHis: 1.611 ± 0.018	4.318AlaIle: 4.318 ± 0.033	3.718AlaLys: 3.718 ± 0.03	6.333AlaLeu: 6.333 ± 0.037	1.706AlaMet: 1.706 ± 0.018	3.279AlaAsn: 3.279 ± 0.026	2.91AlaPro: 2.91 ± 0.03	2.836AlaGln: 2.836 ± 0.032	3.452AlaArg: 3.452 ± 0.025	5.895AlaSer: 5.895 ± 0.039	4.082AlaThr: 4.082 ± 0.033	4.717AlaVal: 4.717 ± 0.032	0.606AlaTrp: 0.606 ± 0.011	1.854AlaTyr: 1.854 ± 0.019	0.009AlaXaa: 0.009 ± 0.001
Cys	1.579CysAla: 1.579 ± 0.02	0.635CysCys: 0.635 ± 0.012	1.319CysAsp: 1.319 ± 0.024	1.395CysGlu: 1.395 ± 0.019	0.959CysPhe: 0.959 ± 0.014	1.366CysGly: 1.366 ± 0.021	0.495CysHis: 0.495 ± 0.012	1.216CysIle: 1.216 ± 0.019	1.03CysLys: 1.03 ± 0.019	1.798CysLeu: 1.798 ± 0.021	0.517CysMet: 0.517 ± 0.01	0.939CysAsn: 0.939 ± 0.018	1.011CysPro: 1.011 ± 0.019	0.718CysGln: 0.718 ± 0.014	1.204CysArg: 1.204 ± 0.016	1.984CysSer: 1.984 ± 0.025	1.103CysThr: 1.103 ± 0.016	1.473CysVal: 1.473 ± 0.019	0.25CysTrp: 0.25 ± 0.007	0.671CysTyr: 0.671 ± 0.012	0.004CysXaa: 0.004 ± 0.001
Asp	3.857AspAla: 3.857 ± 0.03	1.106AspCys: 1.106 ± 0.02	4.574AspAsp: 4.574 ± 0.071	5.211AspGlu: 5.211 ± 0.041	2.137AspPhe: 2.137 ± 0.022	3.676AspGly: 3.676 ± 0.043	1.249AspHis: 1.249 ± 0.015	2.965AspIle: 2.965 ± 0.026	2.716AspLys: 2.716 ± 0.024	4.503AspLeu: 4.503 ± 0.034	1.3AspMet: 1.3 ± 0.015	2.403AspAsn: 2.403 ± 0.024	2.153AspPro: 2.153 ± 0.023	2.056AspGln: 2.056 ± 0.02	3.054AspArg: 3.054 ± 0.027	4.446AspSer: 4.446 ± 0.031	2.483AspThr: 2.483 ± 0.024	3.659AspVal: 3.659 ± 0.033	0.652AspTrp: 0.652 ± 0.013	1.751AspTyr: 1.751 ± 0.018	0.009AspXaa: 0.009 ± 0.001
Glu	4.001GluAla: 4.001 ± 0.034	1.456GluCys: 1.456 ± 0.023	3.469GluAsp: 3.469 ± 0.032	5.124GluGlu: 5.124 ± 0.063	2.183GluPhe: 2.183 ± 0.022	2.885GluGly: 2.885 ± 0.031	1.598GluHis: 1.598 ± 0.017	3.423GluIle: 3.423 ± 0.033	4.244GluLys: 4.244 ± 0.041	5.713GluLeu: 5.713 ± 0.044	1.96GluMet: 1.96 ± 0.02	3.197GluAsn: 3.197 ± 0.028	2.293GluPro: 2.293 ± 0.027	3.163GluGln: 3.163 ± 0.031	4.447GluArg: 4.447 ± 0.041	4.73GluSer: 4.73 ± 0.038	3.441GluThr: 3.441 ± 0.028	3.529GluVal: 3.529 ± 0.031	0.809GluTrp: 0.809 ± 0.013	1.881GluTyr: 1.881 ± 0.021	0.009GluXaa: 0.009 ± 0.001
Phe	2.815PheAla: 2.815 ± 0.025	1.045PheCys: 1.045 ± 0.015	2.64PheAsp: 2.64 ± 0.023	2.516PheGlu: 2.516 ± 0.021	1.859PhePhe: 1.859 ± 0.021	2.701PheGly: 2.701 ± 0.028	1.003PheHis: 1.003 ± 0.014	2.743PheIle: 2.743 ± 0.026	1.825PheLys: 1.825 ± 0.018	3.513PheLeu: 3.513 ± 0.034	1.12PheMet: 1.12 ± 0.013	1.762PheAsn: 1.762 ± 0.02	1.528PhePro: 1.528 ± 0.02	1.451PheGln: 1.451 ± 0.016	2.133PheArg: 2.133 ± 0.021	3.257PheSer: 3.257 ± 0.028	2.231PheThr: 2.231 ± 0.02	3.01PheVal: 3.01 ± 0.025	0.513PheTrp: 0.513 ± 0.011	1.387PheTyr: 1.387 ± 0.018	0.009PheXaa: 0.009 ± 0.001
Gly	3.73GlyAla: 3.73 ± 0.035	1.167GlyCys: 1.167 ± 0.016	3.182GlyAsp: 3.182 ± 0.025	3.255GlyGlu: 3.255 ± 0.035	2.232GlyPhe: 2.232 ± 0.023	4.364GlyGly: 4.364 ± 0.065	1.229GlyHis: 1.229 ± 0.014	3.065GlyIle: 3.065 ± 0.027	2.786GlyLys: 2.786 ± 0.03	4.026GlyLeu: 4.026 ± 0.039	1.406GlyMet: 1.406 ± 0.017	2.409GlyAsn: 2.409 ± 0.022	2.163GlyPro: 2.163 ± 0.053	1.937GlyGln: 1.937 ± 0.025	3.211GlyArg: 3.211 ± 0.036	4.449GlySer: 4.449 ± 0.035	3.14GlyThr: 3.14 ± 0.028	3.494GlyVal: 3.494 ± 0.029	0.677GlyTrp: 0.677 ± 0.013	1.9GlyTyr: 1.9 ± 0.024	0.01GlyXaa: 0.01 ± 0.001
His	1.395HisAla: 1.395 ± 0.015	0.596HisCys: 0.596 ± 0.013	1.119HisAsp: 1.119 ± 0.014	1.344HisGlu: 1.344 ± 0.017	1.228HisPhe: 1.228 ± 0.015	1.16HisGly: 1.16 ± 0.013	0.937HisHis: 0.937 ± 0.017	1.381HisIle: 1.381 ± 0.017	1.094HisLys: 1.094 ± 0.016	2.447HisLeu: 2.447 ± 0.024	0.598HisMet: 0.598 ± 0.01	1.055HisAsn: 1.055 ± 0.012	1.266HisPro: 1.266 ± 0.016	1.199HisGln: 1.199 ± 0.017	1.566HisArg: 1.566 ± 0.019	2.286HisSer: 2.286 ± 0.019	1.089HisThr: 1.089 ± 0.015	1.299HisVal: 1.299 ± 0.014	0.297HisTrp: 0.297 ± 0.007	0.825HisTyr: 0.825 ± 0.011	0.002HisXaa: 0.002 ± 0.001
Ile	4.447IleAla: 4.447 ± 0.032	1.384IleCys: 1.384 ± 0.019	3.634IleAsp: 3.634 ± 0.029	3.675IleGlu: 3.675 ± 0.03	2.409IlePhe: 2.409 ± 0.026	3.268IleGly: 3.268 ± 0.027	1.36IleHis: 1.36 ± 0.018	3.286IleIle: 3.286 ± 0.029	2.666IleLys: 2.666 ± 0.023	4.813IleLeu: 4.813 ± 0.037	1.295IleMet: 1.295 ± 0.016	2.575IleAsn: 2.575 ± 0.02	2.803IlePro: 2.803 ± 0.023	2.156IleGln: 2.156 ± 0.019	3.477IleArg: 3.477 ± 0.025	4.891IleSer: 4.891 ± 0.031	3.252IleThr: 3.252 ± 0.026	3.895IleVal: 3.895 ± 0.027	0.651IleTrp: 0.651 ± 0.012	1.703IleTyr: 1.703 ± 0.017	0.009IleXaa: 0.009 ± 0.001
Lys	3.389LysAla: 3.389 ± 0.027	1.273LysCys: 1.273 ± 0.021	2.645LysAsp: 2.645 ± 0.027	3.721LysGlu: 3.721 ± 0.033	2.011LysPhe: 2.011 ± 0.019	2.54LysGly: 2.54 ± 0.03	1.324LysHis: 1.324 ± 0.018	2.871LysIle: 2.871 ± 0.026	4.122LysLys: 4.122 ± 0.046	5.004LysLeu: 5.004 ± 0.034	1.57LysMet: 1.57 ± 0.017	2.566LysAsn: 2.566 ± 0.024	2.374LysPro: 2.374 ± 0.03	2.63LysGln: 2.63 ± 0.024	4.054LysArg: 4.054 ± 0.031	4.132LysSer: 4.132 ± 0.032	2.962LysThr: 2.962 ± 0.026	3.107LysVal: 3.107 ± 0.027	0.704LysTrp: 0.704 ± 0.012	1.73LysTyr: 1.73 ± 0.018	0.007LysXaa: 0.007 ± 0.001
Leu	5.717LeuAla: 5.717 ± 0.035	1.969LeuCys: 1.969 ± 0.023	4.48LeuAsp: 4.48 ± 0.032	5.023LeuGlu: 5.023 ± 0.043	3.938LeuPhe: 3.938 ± 0.031	3.869LeuGly: 3.869 ± 0.032	2.245LeuHis: 2.245 ± 0.022	5.479LeuIle: 5.479 ± 0.039	5.373LeuLys: 5.373 ± 0.035	8.901LeuLeu: 8.901 ± 0.059	2.44LeuMet: 2.44 ± 0.023	4.54LeuAsn: 4.54 ± 0.032	4.353LeuPro: 4.353 ± 0.034	3.886LeuGln: 3.886 ± 0.032	5.413LeuArg: 5.413 ± 0.03	7.924LeuSer: 7.924 ± 0.045	4.711LeuThr: 4.711 ± 0.032	4.931LeuVal: 4.931 ± 0.036	0.934LeuTrp: 0.934 ± 0.013	2.518LeuTyr: 2.518 ± 0.025	0.018LeuXaa: 0.018 ± 0.002
Met	1.827MetAla: 1.827 ± 0.019	0.538MetCys: 0.538 ± 0.011	1.399MetAsp: 1.399 ± 0.017	1.516MetGlu: 1.516 ± 0.018	1.014MetPhe: 1.014 ± 0.014	1.264MetGly: 1.264 ± 0.018	0.645MetHis: 0.645 ± 0.011	1.519MetIle: 1.519 ± 0.02	1.658MetLys: 1.658 ± 0.017	2.408MetLeu: 2.408 ± 0.026	0.815MetMet: 0.815 ± 0.018	1.494MetAsn: 1.494 ± 0.018	1.203MetPro: 1.203 ± 0.014	1.196MetGln: 1.196 ± 0.014	1.615MetArg: 1.615 ± 0.016	2.042MetSer: 2.042 ± 0.021	1.345MetThr: 1.345 ± 0.018	1.476MetVal: 1.476 ± 0.016	0.254MetTrp: 0.254 ± 0.007	0.614MetTyr: 0.614 ± 0.011	0.004MetXaa: 0.004 ± 0.001
Asn	3.864AsnAla: 3.864 ± 0.027	0.983AsnCys: 0.983 ± 0.016	3.231AsnAsp: 3.231 ± 0.027	3.677AsnGlu: 3.677 ± 0.032	1.839AsnPhe: 1.839 ± 0.022	3.246AsnGly: 3.246 ± 0.034	1.016AsnHis: 1.016 ± 0.015	2.725AsnIle: 2.725 ± 0.021	2.295AsnLys: 2.295 ± 0.023	3.68AsnLeu: 3.68 ± 0.028	1.096AsnMet: 1.096 ± 0.013	2.938AsnAsn: 2.938 ± 0.038	1.953AsnPro: 1.953 ± 0.021	1.821AsnGln: 1.821 ± 0.022	2.673AsnArg: 2.673 ± 0.023	3.902AsnSer: 3.902 ± 0.031	2.463AsnThr: 2.463 ± 0.025	3.21AsnVal: 3.21 ± 0.025	0.498AsnTrp: 0.498 ± 0.009	1.367AsnTyr: 1.367 ± 0.017	0.007AsnXaa: 0.007 ± 0.001
Pro	2.753ProAla: 2.753 ± 0.027	0.732ProCys: 0.732 ± 0.014	2.188ProAsp: 2.188 ± 0.023	2.605ProGlu: 2.605 ± 0.028	1.873ProPhe: 1.873 ± 0.022	2.491ProGly: 2.491 ± 0.072	1.037ProHis: 1.037 ± 0.014	2.545ProIle: 2.545 ± 0.026	2.406ProLys: 2.406 ± 0.024	3.934ProLeu: 3.934 ± 0.03	0.989ProMet: 0.989 ± 0.015	2.163ProAsn: 2.163 ± 0.021	3.591ProPro: 3.591 ± 0.056	2.099ProGln: 2.099 ± 0.025	2.193ProArg: 2.193 ± 0.023	4.785ProSer: 4.785 ± 0.035	2.862ProThr: 2.862 ± 0.029	2.708ProVal: 2.708 ± 0.027	0.407ProTrp: 0.407 ± 0.009	1.392ProTyr: 1.392 ± 0.018	0.008ProXaa: 0.008 ± 0.001
Gln	2.349GlnAla: 2.349 ± 0.026	0.965GlnCys: 0.965 ± 0.018	1.347GlnAsp: 1.347 ± 0.018	1.999GlnGlu: 1.999 ± 0.023	1.772GlnPhe: 1.772 ± 0.02	1.544GlnGly: 1.544 ± 0.019	1.219GlnHis: 1.219 ± 0.014	2.483GlnIle: 2.483 ± 0.022	2.547GlnLys: 2.547 ± 0.024	4.675GlnLeu: 4.675 ± 0.036	1.363GlnMet: 1.363 ± 0.018	1.958GlnAsn: 1.958 ± 0.022	2.236GlnPro: 2.236 ± 0.027	3.98GlnGln: 3.98 ± 0.065	2.79GlnArg: 2.79 ± 0.029	3.547GlnSer: 3.547 ± 0.032	2.276GlnThr: 2.276 ± 0.023	2.197GlnVal: 2.197 ± 0.02	0.488GlnTrp: 0.488 ± 0.009	1.259GlnTyr: 1.259 ± 0.016	0.009GlnXaa: 0.009 ± 0.001
Arg	3.517ArgAla: 3.517 ± 0.029	1.384ArgCys: 1.384 ± 0.024	2.893ArgAsp: 2.893 ± 0.024	3.488ArgGlu: 3.488 ± 0.031	2.624ArgPhe: 2.624 ± 0.024	2.758ArgGly: 2.758 ± 0.029	1.491ArgHis: 1.491 ± 0.016	3.648ArgIle: 3.648 ± 0.03	3.705ArgLys: 3.705 ± 0.031	5.607ArgLeu: 5.607 ± 0.032	1.629ArgMet: 1.629 ± 0.018	2.946ArgAsn: 2.946 ± 0.022	2.378ArgPro: 2.378 ± 0.026	2.456ArgGln: 2.456 ± 0.021	4.769ArgArg: 4.769 ± 0.04	5.092ArgSer: 5.092 ± 0.035	3.047ArgThr: 3.047 ± 0.022	3.255ArgVal: 3.255 ± 0.023	0.687ArgTrp: 0.687 ± 0.011	1.922ArgTyr: 1.922 ± 0.019	0.008ArgXaa: 0.008 ± 0.001
Ser	6.527SerAla: 6.527 ± 0.043	1.659SerCys: 1.659 ± 0.021	4.999SerAsp: 4.999 ± 0.041	5.113SerGlu: 5.113 ± 0.037	3.436SerPhe: 3.436 ± 0.028	4.807SerGly: 4.807 ± 0.036	1.839SerHis: 1.839 ± 0.018	4.584SerIle: 4.584 ± 0.037	4.391SerLys: 4.391 ± 0.034	7.278SerLeu: 7.278 ± 0.043	2.042SerMet: 2.042 ± 0.02	4.499SerAsn: 4.499 ± 0.035	3.995SerPro: 3.995 ± 0.04	3.269SerGln: 3.269 ± 0.029	4.496SerArg: 4.496 ± 0.03	11.042SerSer: 11.042 ± 0.102	5.83SerThr: 5.83 ± 0.053	5.495SerVal: 5.495 ± 0.028	0.732SerTrp: 0.732 ± 0.013	2.113SerTyr: 2.113 ± 0.021	0.016SerXaa: 0.016 ± 0.002
Thr	4.35ThrAla: 4.35 ± 0.034	1.021ThrCys: 1.021 ± 0.018	2.929ThrAsp: 2.929 ± 0.033	3.145ThrGlu: 3.145 ± 0.03	2.214ThrPhe: 2.214 ± 0.021	2.827ThrGly: 2.827 ± 0.028	1.217ThrHis: 1.217 ± 0.017	3.344ThrIle: 3.344 ± 0.024	2.871ThrLys: 2.871 ± 0.029	4.734ThrLeu: 4.734 ± 0.03	1.296ThrMet: 1.296 ± 0.016	2.855ThrAsn: 2.855 ± 0.024	2.984ThrPro: 2.984 ± 0.03	2.069ThrGln: 2.069 ± 0.023	2.618ThrArg: 2.618 ± 0.022	5.496ThrSer: 5.496 ± 0.044	4.363ThrThr: 4.363 ± 0.058	3.848ThrVal: 3.848 ± 0.03	0.457ThrTrp: 0.457 ± 0.01	1.445ThrTyr: 1.445 ± 0.019	0.009ThrXaa: 0.009 ± 0.001
Val	4.163ValAla: 4.163 ± 0.032	1.539ValCys: 1.539 ± 0.019	3.905ValAsp: 3.905 ± 0.034	3.975ValGlu: 3.975 ± 0.033	2.626ValPhe: 2.626 ± 0.029	3.181ValGly: 3.181 ± 0.031	1.594ValHis: 1.594 ± 0.018	3.803ValIle: 3.803 ± 0.026	3.228ValLys: 3.228 ± 0.029	5.469ValLeu: 5.469 ± 0.035	1.537ValMet: 1.537 ± 0.017	3.019ValAsn: 3.019 ± 0.024	2.812ValPro: 2.812 ± 0.026	2.432ValGln: 2.432 ± 0.023	3.463ValArg: 3.463 ± 0.028	5.017ValSer: 5.017 ± 0.034	3.357ValThr: 3.357 ± 0.026	4.346ValVal: 4.346 ± 0.033	0.685ValTrp: 0.685 ± 0.012	1.873ValTyr: 1.873 ± 0.02	0.011ValXaa: 0.011 ± 0.001
Trp	0.602TrpAla: 0.602 ± 0.009	0.22TrpCys: 0.22 ± 0.007	0.542TrpAsp: 0.542 ± 0.009	0.522TrpGlu: 0.522 ± 0.01	0.487TrpPhe: 0.487 ± 0.011	0.438TrpGly: 0.438 ± 0.01	0.279TrpHis: 0.279 ± 0.007	0.736TrpIle: 0.736 ± 0.011	0.685TrpLys: 0.685 ± 0.012	1.125TrpLeu: 1.125 ± 0.016	0.391TrpMet: 0.391 ± 0.009	0.635TrpAsn: 0.635 ± 0.011	0.446TrpPro: 0.446 ± 0.01	0.464TrpGln: 0.464 ± 0.009	0.734TrpArg: 0.734 ± 0.012	0.9TrpSer: 0.9 ± 0.015	0.632TrpThr: 0.632 ± 0.011	0.519TrpVal: 0.519 ± 0.009	0.162TrpTrp: 0.162 ± 0.006	0.343TrpTyr: 0.343 ± 0.009	0.002TrpXaa: 0.002 ± 0.001
Tyr	1.972TyrAla: 1.972 ± 0.021	0.795TyrCys: 0.795 ± 0.014	1.761TyrAsp: 1.761 ± 0.02	1.883TyrGlu: 1.883 ± 0.02	1.394TyrPhe: 1.394 ± 0.016	1.958TyrGly: 1.958 ± 0.024	0.79TyrHis: 0.79 ± 0.012	1.508TyrIle: 1.508 ± 0.018	1.368TyrLys: 1.368 ± 0.016	2.687TyrLeu: 2.687 ± 0.026	0.77TyrMet: 0.77 ± 0.012	1.246TyrAsn: 1.246 ± 0.016	1.304TyrPro: 1.304 ± 0.018	1.177TyrGln: 1.177 ± 0.015	1.933TyrArg: 1.933 ± 0.021	2.328TyrSer: 2.328 ± 0.021	1.414TyrThr: 1.414 ± 0.018	1.858TyrVal: 1.858 ± 0.021	0.401TyrTrp: 0.401 ± 0.01	1.17TyrTyr: 1.17 ± 0.017	0.005TyrXaa: 0.005 ± 0.001
Xaa	0.009XaaAla: 0.009 ± 0.001	0.005XaaCys: 0.005 ± 0.001	0.007XaaAsp: 0.007 ± 0.001	0.011XaaGlu: 0.011 ± 0.002	0.014XaaPhe: 0.014 ± 0.001	0.009XaaGly: 0.009 ± 0.001	0.006XaaHis: 0.006 ± 0.001	0.007XaaIle: 0.007 ± 0.001	0.007XaaLys: 0.007 ± 0.001	0.014XaaLeu: 0.014 ± 0.002	0.007XaaMet: 0.007 ± 0.001	0.007XaaAsn: 0.007 ± 0.001	0.006XaaPro: 0.006 ± 0.001	0.006XaaGln: 0.006 ± 0.001	0.009XaaArg: 0.009 ± 0.001	0.016XaaSer: 0.016 ± 0.002	0.008XaaThr: 0.008 ± 0.001	0.01XaaVal: 0.01 ± 0.001	0.001XaaTrp: 0.001 ± 0.0	0.004XaaTyr: 0.004 ± 0.001	0.229XaaXaa: 0.229 ± 0.031

Statistics based on 20779 proteins (5639772 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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