Loktanella sp. IMCC34160
Average proteome isoelectric point is 6.01
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3606 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4Q3Y3L4|A0A4Q3Y3L4_9RHOB Uncharacterized protein OS=Loktanella sp. IMCC34160 OX=2510646 GN=EU803_16140 PE=4 SV=1
MM1 pKa = 7.81 KK2 pKa = 9.62 STLKK6 pKa = 9.97 LTTALVFGACLSSAAAAQTEE26 pKa = 4.41 LTMWYY31 pKa = 9.3 HH32 pKa = 5.74 GAGNEE37 pKa = 4.11 VEE39 pKa = 4.85 SNIINQIVSDD49 pKa = 4.44 FNASQSDD56 pKa = 3.69 WVVSLEE62 pKa = 4.12 SFPQGAYY69 pKa = 9.97 NDD71 pKa = 4.3 SVVAAALAGNLPDD84 pKa = 4.5 ILDD87 pKa = 3.29 VDD89 pKa = 4.65 GPVMPNWAWAGYY101 pKa = 7.19 MQPLPIDD108 pKa = 3.4 EE109 pKa = 4.52 SVIEE113 pKa = 4.27 NFLPGPKK120 pKa = 10.05 GYY122 pKa = 9.55 WDD124 pKa = 3.9 GEE126 pKa = 4.41 LYY128 pKa = 10.91 SIGLWDD134 pKa = 3.83 AAVALVTRR142 pKa = 11.84 QSTLDD147 pKa = 3.51 ALGLRR152 pKa = 11.84 TPTLEE157 pKa = 4.3 EE158 pKa = 3.82 PWNLQEE164 pKa = 6.22 FMAALDD170 pKa = 3.64 AAKK173 pKa = 10.53 ASGEE177 pKa = 3.72 FDD179 pKa = 3.65 YY180 pKa = 11.42 PLDD183 pKa = 5.52 LGMAWTGEE191 pKa = 3.99 WYY193 pKa = 9.24 PYY195 pKa = 10.83 AFSPFLQSFGGDD207 pKa = 2.53 IVDD210 pKa = 3.72 RR211 pKa = 11.84 STYY214 pKa = 8.3 QTAEE218 pKa = 3.77 GALNGDD224 pKa = 3.34 AAMAFGEE231 pKa = 4.18 WWQGLFANGYY241 pKa = 10.52 APGTSQDD248 pKa = 3.4 PADD251 pKa = 4.57 RR252 pKa = 11.84 DD253 pKa = 3.59 GGFIDD258 pKa = 4.61 GKK260 pKa = 10.73 YY261 pKa = 9.74 AFSWNGNWAALGALNAHH278 pKa = 7.49 DD279 pKa = 4.61 DD280 pKa = 4.16 TLFLPAPDD288 pKa = 4.64 FGNGSTIGAASWQFGVSASSEE309 pKa = 4.27 HH310 pKa = 6.93 PEE312 pKa = 3.73 GAAAFIQFALQDD324 pKa = 3.28 QYY326 pKa = 12.04 LADD329 pKa = 4.53 FSNGIGLIPATASSAQMTEE348 pKa = 3.86 NYY350 pKa = 10.76 ADD352 pKa = 4.33 GGPMAVFFEE361 pKa = 4.43 LTEE364 pKa = 4.14 AQALVRR370 pKa = 11.84 PVTPGYY376 pKa = 10.01 VVQAKK381 pKa = 9.65 VFEE384 pKa = 4.37 KK385 pKa = 10.94 ALADD389 pKa = 3.53 IANGADD395 pKa = 3.38 VADD398 pKa = 4.21 TLDD401 pKa = 3.76 AAVDD405 pKa = 4.3 EE406 pKa = 4.72 INADD410 pKa = 2.98 IAANNGYY417 pKa = 9.04 GHH419 pKa = 7.6
Molecular weight: 44.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.724
IPC2_protein 3.668
IPC_protein 3.694
Toseland 3.465
ProMoST 3.846
Dawson 3.681
Bjellqvist 3.834
Wikipedia 3.617
Rodwell 3.516
Grimsley 3.376
Solomon 3.681
Lehninger 3.63
Nozaki 3.795
DTASelect 4.037
Thurlkill 3.516
EMBOSS 3.63
Sillero 3.808
Patrickios 0.744
IPC_peptide 3.668
IPC2_peptide 3.783
IPC2.peptide.svr19 3.74
Protein with the highest isoelectric point:
>tr|A0A4Q3YH39|A0A4Q3YH39_9RHOB Ornithine carbamoyltransferase OS=Loktanella sp. IMCC34160 OX=2510646 GN=argF PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNRR10 pKa = 11.84 VRR12 pKa = 11.84 KK13 pKa = 8.99 NRR15 pKa = 11.84 HH16 pKa = 3.77 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.37 AGRR28 pKa = 11.84 KK29 pKa = 8.54 ILNARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 9.07 SLSAA44 pKa = 3.93
Molecular weight: 5.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3606
0
3606
1135054
28
2415
314.8
34.13
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.166 ± 0.057
0.89 ± 0.013
6.381 ± 0.046
5.764 ± 0.04
3.782 ± 0.026
8.994 ± 0.047
2.03 ± 0.02
5.25 ± 0.03
3.001 ± 0.036
9.787 ± 0.052
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.734 ± 0.022
2.626 ± 0.025
5.129 ± 0.03
3.068 ± 0.021
6.704 ± 0.04
5.057 ± 0.033
5.557 ± 0.032
7.364 ± 0.034
1.449 ± 0.016
2.268 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here