Dankookia rubra
Average proteome isoelectric point is 7.13
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7087 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4V3AA02|A0A4V3AA02_9PROT IS21 family transposase OS=Dankookia rubra OX=1442381 GN=E2C06_17690 PE=4 SV=1
MM1 pKa = 7.71 SGTTSNTAFGADD13 pKa = 3.96 DD14 pKa = 3.78 SAIPAAIGGGGSAGPSVGAGDD35 pKa = 3.6 ATGPIVSVNTSGIDD49 pKa = 3.66 LAGLPAQYY57 pKa = 9.26 STASADD63 pKa = 3.57 GTGLGFFTRR72 pKa = 11.84 QLEE75 pKa = 4.32 YY76 pKa = 10.97 VPTAVTVGPDD86 pKa = 3.14 DD87 pKa = 3.92 ALYY90 pKa = 10.89 VSGLSGLPYY99 pKa = 9.16 PDD101 pKa = 4.12 GYY103 pKa = 10.61 SRR105 pKa = 11.84 VMRR108 pKa = 11.84 VADD111 pKa = 3.6 THH113 pKa = 5.97 ATTGFDD119 pKa = 3.56 GQIDD123 pKa = 3.73 SGVPQTYY130 pKa = 10.6 ASGLSQVNGLSFDD143 pKa = 3.69 GKK145 pKa = 11.2 GNLNVLEE152 pKa = 4.55 FVNAANIYY160 pKa = 10.62 DD161 pKa = 4.1 PTRR164 pKa = 11.84 QPGEE168 pKa = 4.23 LPPSEE173 pKa = 5.59 LIRR176 pKa = 11.84 IAPDD180 pKa = 3.55 GVRR183 pKa = 11.84 TTISGPEE190 pKa = 3.95 LKK192 pKa = 10.43 LANYY196 pKa = 9.37 VLADD200 pKa = 3.67 KK201 pKa = 8.78 EE202 pKa = 4.5 TGDD205 pKa = 3.69 VYY207 pKa = 11.37 VAIGNADD214 pKa = 3.36 INNGEE219 pKa = 4.29 VLRR222 pKa = 11.84 YY223 pKa = 9.58 HH224 pKa = 7.09 VDD226 pKa = 3.45 DD227 pKa = 4.09 ATGQATSVDD236 pKa = 4.09 VVASGLNNPRR246 pKa = 11.84 GMAFGPDD253 pKa = 2.51 GHH255 pKa = 8.37 LYY257 pKa = 10.97 VNEE260 pKa = 4.22 QGLGTPGDD268 pKa = 4.04 SPDD271 pKa = 3.54 AATAPTIPFIPGAVDD286 pKa = 3.12 EE287 pKa = 5.15 RR288 pKa = 11.84 GGFTASITRR297 pKa = 11.84 VDD299 pKa = 3.24 IDD301 pKa = 3.43 GSGQEE306 pKa = 4.65 RR307 pKa = 11.84 IFTGLPSIRR316 pKa = 11.84 EE317 pKa = 3.98 VNAATGEE324 pKa = 4.0 DD325 pKa = 3.52 RR326 pKa = 11.84 VISVGSNGFTIAPDD340 pKa = 3.47 GTAYY344 pKa = 10.86 LATGGGLTAATADD357 pKa = 3.76 ALGPLAPYY365 pKa = 9.96 IQGLLKK371 pKa = 9.9 IDD373 pKa = 3.84 GLFDD377 pKa = 4.64 HH378 pKa = 7.52 DD379 pKa = 4.47 PSNVTVTPAFNSVTYY394 pKa = 10.23 AAEE397 pKa = 4.23 NGPDD401 pKa = 3.64 GSQTLFNTQSNLNDD415 pKa = 3.33 VVVGSDD421 pKa = 2.79 GHH423 pKa = 7.93 IYY425 pKa = 10.73 AVDD428 pKa = 3.25 AARR431 pKa = 11.84 NVMYY435 pKa = 10.51 GFSPDD440 pKa = 4.35 DD441 pKa = 3.83 LSTPDD446 pKa = 3.72 SVTVFQKK453 pKa = 10.17 QAPVLTPPQYY463 pKa = 10.86 AAVVAAGGNPSLDD476 pKa = 3.65 YY477 pKa = 10.56 QVEE480 pKa = 4.33 IAQTTYY486 pKa = 10.64 KK487 pKa = 10.6 AEE489 pKa = 4.28 NGAPDD494 pKa = 3.7 TPGRR498 pKa = 11.84 AEE500 pKa = 3.84 AAASFSPADD509 pKa = 3.97 PGAAAAGAPAGPADD523 pKa = 4.02 AGLPAGIVDD532 pKa = 3.72 TAVPPRR538 pKa = 11.84 GEE540 pKa = 4.19 DD541 pKa = 3.27 ASVGTVNAAGADD553 pKa = 3.93 GLPGSIPVTDD563 pKa = 4.28 PSFPGPIDD571 pKa = 4.69 PISPPVLPGNVYY583 pKa = 10.09 VPYY586 pKa = 10.13 FDD588 pKa = 4.79 PFFGGNYY595 pKa = 10.01 APATPPVLPAGGHH608 pKa = 5.46 GATYY612 pKa = 9.22 TVSHH616 pKa = 7.13 LYY618 pKa = 10.8 SFGDD622 pKa = 3.49 RR623 pKa = 11.84 LVDD626 pKa = 4.28 DD627 pKa = 4.7 GGSYY631 pKa = 10.14 GAAAVAQAAGQPAPNASPVYY651 pKa = 8.49 YY652 pKa = 10.25 QGGFSDD658 pKa = 4.41 GPNWTDD664 pKa = 3.13 NLAKK668 pKa = 10.36 ILGAQQTDD676 pKa = 3.85 GEE678 pKa = 4.9 DD679 pKa = 3.35 NNFGYY684 pKa = 10.85 VLATARR690 pKa = 11.84 PIEE693 pKa = 4.4 NPLDD697 pKa = 3.99 PFTGQTTLNTFEE709 pKa = 4.18 GQIDD713 pKa = 4.19 AFQQAHH719 pKa = 6.82 AYY721 pKa = 9.94 FSANDD726 pKa = 3.72 LVTVTFGGNDD736 pKa = 3.17 LTLPSNLSPEE746 pKa = 4.16 EE747 pKa = 4.74 GITEE751 pKa = 4.19 SVQAIVDD758 pKa = 3.86 GLDD761 pKa = 2.97 RR762 pKa = 11.84 LADD765 pKa = 4.15 LGAHH769 pKa = 6.19 HH770 pKa = 6.94 FVITNLPDD778 pKa = 3.91 LEE780 pKa = 4.37 LAPLFKK786 pKa = 11.06 DD787 pKa = 3.65 PDD789 pKa = 3.74 FLQQLGAEE797 pKa = 4.48 PGSFQPLVAEE807 pKa = 5.05 FNARR811 pKa = 11.84 LADD814 pKa = 3.61 AVHH817 pKa = 6.39 AFEE820 pKa = 4.97 GEE822 pKa = 4.0 RR823 pKa = 11.84 GVDD826 pKa = 3.38 VTLLDD831 pKa = 3.51 VHH833 pKa = 7.21 KK834 pKa = 10.57 LFDD837 pKa = 6.01 AIADD841 pKa = 3.77 DD842 pKa = 3.87 PAAYY846 pKa = 10.03 GFNDD850 pKa = 2.86 VDD852 pKa = 4.11 QPVLAAPPLQPGTPTVYY869 pKa = 10.41 NPAIVGQDD877 pKa = 3.36 PAVEE881 pKa = 4.05 HH882 pKa = 6.88 ASLFIDD888 pKa = 4.14 PFFHH892 pKa = 6.61 PTALGQAILAEE903 pKa = 4.52 TAWHH907 pKa = 6.4 VIAA910 pKa = 5.91
Molecular weight: 93.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.721
IPC2_protein 3.821
IPC_protein 3.872
Toseland 3.643
ProMoST 4.037
Dawson 3.872
Bjellqvist 4.024
Wikipedia 3.821
Rodwell 3.694
Grimsley 3.541
Solomon 3.872
Lehninger 3.834
Nozaki 3.973
DTASelect 4.266
Thurlkill 3.694
EMBOSS 3.834
Sillero 3.999
Patrickios 1.914
IPC_peptide 3.872
IPC2_peptide 3.973
IPC2.peptide.svr19 3.879
Protein with the highest isoelectric point:
>tr|A0A4R5Q7N2|A0A4R5Q7N2_9PROT PaaI family thioesterase OS=Dankookia rubra OX=1442381 GN=E2C06_32165 PE=4 SV=1
MM1 pKa = 7.59 PPAPARR7 pKa = 11.84 AAGRR11 pKa = 11.84 APAAAPRR18 pKa = 11.84 GRR20 pKa = 11.84 ARR22 pKa = 11.84 GGGRR26 pKa = 11.84 TAPARR31 pKa = 11.84 PSAPPAPAPAWSRR44 pKa = 11.84 RR45 pKa = 11.84 APRR48 pKa = 11.84 PAGPAPPLAPPGPAPGRR65 pKa = 11.84 RR66 pKa = 11.84 PRR68 pKa = 3.85
Molecular weight: 6.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.301
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.554
Rodwell 12.574
Grimsley 13.115
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.31
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.257
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7087
0
7087
2193505
25
3143
309.5
33.15
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
15.024 ± 0.05
0.908 ± 0.01
5.042 ± 0.022
5.335 ± 0.025
3.211 ± 0.016
9.512 ± 0.029
2.047 ± 0.014
3.893 ± 0.018
2.023 ± 0.022
10.911 ± 0.038
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.294 ± 0.014
1.984 ± 0.015
6.364 ± 0.025
3.064 ± 0.018
8.445 ± 0.033
4.332 ± 0.019
4.944 ± 0.02
7.31 ± 0.026
1.567 ± 0.013
1.79 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here