Amycolatopsis pithecelloba

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Pseudonocardiales; Pseudonocardiaceae;

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7322 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6N7YR11|A0A6N7YR11_9PSEU Methionine synthase OS=Amycolatopsis pithecelloba OX=664692 GN=metH PE=3 SV=1
MM1 pKa = 7.31SHH3 pKa = 7.87PIHH6 pKa = 6.31TLHH9 pKa = 7.07SLVLNLVTDD18 pKa = 4.12HH19 pKa = 7.02AFGSAFLANPTAVLSEE35 pKa = 4.58AGLTDD40 pKa = 3.0ITGADD45 pKa = 3.47VSEE48 pKa = 4.51VTSLVAEE55 pKa = 4.37HH56 pKa = 6.46VPAPVADD63 pKa = 3.56AVEE66 pKa = 4.47NGLASLPTDD75 pKa = 3.21ALGVNDD81 pKa = 5.04LNSALAHH88 pKa = 6.14LEE90 pKa = 4.06VVATVAQEE98 pKa = 4.48VPATAASTSDD108 pKa = 3.43FALGSVSGVTEE119 pKa = 4.01ATAVSSSFGATADD132 pKa = 3.7GLATSLAAATPVGDD146 pKa = 3.43VTGAVAASAGTLGVGLSSSAGAYY169 pKa = 9.31GVGVGSLPLSAPSFDD184 pKa = 3.76SVGDD188 pKa = 3.79LGSTLDD194 pKa = 3.66SDD196 pKa = 4.54VFSDD200 pKa = 4.23ASPVSSTATGYY211 pKa = 10.68VDD213 pKa = 4.36QGVDD217 pKa = 3.42LAGSGVTGVTAFAGDD232 pKa = 4.7HH233 pKa = 6.05IAGAGAGLGEE243 pKa = 4.39TVAATGADD251 pKa = 3.63VAGHH255 pKa = 6.32LTSVPAVAAQATSVLPHH272 pKa = 5.09VTAPVHH278 pKa = 6.44LPADD282 pKa = 4.18LPVHH286 pKa = 6.32LAAEE290 pKa = 4.7LPHH293 pKa = 7.38LPAAAAVPNVPDD305 pKa = 3.78TVEE308 pKa = 4.22DD309 pKa = 4.24TVSHH313 pKa = 5.78ATSVTGSVTHH323 pKa = 7.0GGLLDD328 pKa = 3.73TAHH331 pKa = 7.33AALPDD336 pKa = 3.68TGDD339 pKa = 3.15LSGNLLHH346 pKa = 7.06TDD348 pKa = 4.13LPLGHH353 pKa = 7.24

Molecular weight: 33.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6N7YYR3|A0A6N7YYR3_9PSEU LLM class flavin-dependent oxidoreductase OS=Amycolatopsis pithecelloba OX=664692 GN=GKO32_24125 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.38KK7 pKa = 8.42RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.06RR11 pKa = 11.84MSKK14 pKa = 9.76KK15 pKa = 9.54KK16 pKa = 9.72HH17 pKa = 5.63RR18 pKa = 11.84KK19 pKa = 7.68LLRR22 pKa = 11.84RR23 pKa = 11.84TRR25 pKa = 11.84MQRR28 pKa = 11.84RR29 pKa = 11.84KK30 pKa = 9.69QGKK33 pKa = 8.53

Molecular weight: 4.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7322

0

7322

2356100

29

29510

321.8

34.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.969 ± 0.045

0.77 ± 0.014

5.916 ± 0.032

5.701 ± 0.026

3.001 ± 0.017

9.14 ± 0.029

2.301 ± 0.014

3.837 ± 0.023

2.121 ± 0.019

10.392 ± 0.032

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.762 ± 0.011

2.012 ± 0.017

5.825 ± 0.03

2.92 ± 0.024

7.888 ± 0.037

5.264 ± 0.019

5.908 ± 0.023

8.742 ± 0.033

1.488 ± 0.012

2.043 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski