Amino acid dipepetide frequency for Streptomyces phage LilMartin

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.363AlaAla: 7.363 ± 0.848
0.931AlaCys: 0.931 ± 0.222
5.093AlaAsp: 5.093 ± 0.393
5.588AlaGlu: 5.588 ± 0.517
2.765AlaPhe: 2.765 ± 0.33
5.559AlaGly: 5.559 ± 0.597
1.601AlaHis: 1.601 ± 0.199
5.006AlaIle: 5.006 ± 0.453
5.064AlaLys: 5.064 ± 0.417
6.956AlaLeu: 6.956 ± 0.548
2.532AlaMet: 2.532 ± 0.348
2.881AlaAsn: 2.881 ± 0.407
2.852AlaPro: 2.852 ± 0.401
2.91AlaGln: 2.91 ± 0.352
4.86AlaArg: 4.86 ± 0.391
4.715AlaSer: 4.715 ± 0.496
5.326AlaThr: 5.326 ± 0.746
5.559AlaVal: 5.559 ± 0.45
1.601AlaTrp: 1.601 ± 0.195
3.492AlaTyr: 3.492 ± 0.305
0.0AlaXaa: 0.0 ± 0.0
Cys
0.437CysAla: 0.437 ± 0.125
0.204CysCys: 0.204 ± 0.087
0.698CysAsp: 0.698 ± 0.186
0.495CysGlu: 0.495 ± 0.131
0.349CysPhe: 0.349 ± 0.096
1.135CysGly: 1.135 ± 0.201
0.204CysHis: 0.204 ± 0.074
0.582CysIle: 0.582 ± 0.136
0.786CysLys: 0.786 ± 0.152
0.669CysLeu: 0.669 ± 0.162
0.291CysMet: 0.291 ± 0.098
0.786CysAsn: 0.786 ± 0.161
0.582CysPro: 0.582 ± 0.138
0.175CysGln: 0.175 ± 0.082
0.728CysArg: 0.728 ± 0.15
0.524CysSer: 0.524 ± 0.151
0.407CysThr: 0.407 ± 0.107
0.495CysVal: 0.495 ± 0.12
0.146CysTrp: 0.146 ± 0.068
0.349CysTyr: 0.349 ± 0.1
0.0CysXaa: 0.0 ± 0.0
Asp
5.675AspAla: 5.675 ± 0.455
0.553AspCys: 0.553 ± 0.126
3.987AspAsp: 3.987 ± 0.398
5.238AspGlu: 5.238 ± 0.449
3.172AspPhe: 3.172 ± 0.316
5.326AspGly: 5.326 ± 0.535
0.96AspHis: 0.96 ± 0.185
3.667AspIle: 3.667 ± 0.397
3.987AspLys: 3.987 ± 0.393
4.802AspLeu: 4.802 ± 0.413
2.008AspMet: 2.008 ± 0.243
3.085AspAsn: 3.085 ± 0.288
2.27AspPro: 2.27 ± 0.247
1.542AspGln: 1.542 ± 0.181
2.736AspArg: 2.736 ± 0.269
3.987AspSer: 3.987 ± 0.322
3.405AspThr: 3.405 ± 0.397
4.656AspVal: 4.656 ± 0.414
1.659AspTrp: 1.659 ± 0.244
2.765AspTyr: 2.765 ± 0.336
0.0AspXaa: 0.0 ± 0.0
Glu
6.257GluAla: 6.257 ± 0.554
0.524GluCys: 0.524 ± 0.15
4.744GluAsp: 4.744 ± 0.39
4.365GluGlu: 4.365 ± 0.517
2.998GluPhe: 2.998 ± 0.307
4.482GluGly: 4.482 ± 0.402
1.659GluHis: 1.659 ± 0.282
4.307GluIle: 4.307 ± 0.368
4.54GluLys: 4.54 ± 0.452
5.355GluLeu: 5.355 ± 0.422
2.154GluMet: 2.154 ± 0.251
2.852GluAsn: 2.852 ± 0.316
2.386GluPro: 2.386 ± 0.332
2.736GluGln: 2.736 ± 0.314
3.958GluArg: 3.958 ± 0.409
3.405GluSer: 3.405 ± 0.349
3.754GluThr: 3.754 ± 0.428
5.18GluVal: 5.18 ± 0.477
1.484GluTrp: 1.484 ± 0.233
2.852GluTyr: 2.852 ± 0.347
0.0GluXaa: 0.0 ± 0.0
Phe
2.474PheAla: 2.474 ± 0.293
0.378PheCys: 0.378 ± 0.112
3.434PheAsp: 3.434 ± 0.271
3.492PheGlu: 3.492 ± 0.309
1.572PhePhe: 1.572 ± 0.214
3.23PheGly: 3.23 ± 0.269
0.728PheHis: 0.728 ± 0.163
1.892PheIle: 1.892 ± 0.228
2.154PheLys: 2.154 ± 0.271
1.979PheLeu: 1.979 ± 0.235
1.281PheMet: 1.281 ± 0.169
2.066PheAsn: 2.066 ± 0.223
1.048PhePro: 1.048 ± 0.176
1.135PheGln: 1.135 ± 0.191
1.979PheArg: 1.979 ± 0.241
2.619PheSer: 2.619 ± 0.316
2.561PheThr: 2.561 ± 0.285
2.619PheVal: 2.619 ± 0.345
0.64PheTrp: 0.64 ± 0.176
1.281PheTyr: 1.281 ± 0.155
0.0PheXaa: 0.0 ± 0.0
Gly
5.093GlyAla: 5.093 ± 0.437
0.553GlyCys: 0.553 ± 0.116
4.802GlyAsp: 4.802 ± 0.402
4.22GlyGlu: 4.22 ± 0.33
3.23GlyPhe: 3.23 ± 0.319
5.471GlyGly: 5.471 ± 0.507
1.659GlyHis: 1.659 ± 0.214
4.511GlyIle: 4.511 ± 0.337
5.268GlyLys: 5.268 ± 0.476
5.704GlyLeu: 5.704 ± 0.567
2.794GlyMet: 2.794 ± 0.296
3.405GlyAsn: 3.405 ± 0.319
2.27GlyPro: 2.27 ± 0.325
2.823GlyGln: 2.823 ± 0.34
3.987GlyArg: 3.987 ± 0.394
4.715GlySer: 4.715 ± 0.502
5.5GlyThr: 5.5 ± 0.69
5.966GlyVal: 5.966 ± 0.446
1.368GlyTrp: 1.368 ± 0.222
3.172GlyTyr: 3.172 ± 0.321
0.0GlyXaa: 0.0 ± 0.0
His
1.077HisAla: 1.077 ± 0.2
0.291HisCys: 0.291 ± 0.087
1.106HisAsp: 1.106 ± 0.182
1.106HisGlu: 1.106 ± 0.186
0.815HisPhe: 0.815 ± 0.147
1.688HisGly: 1.688 ± 0.252
0.495HisHis: 0.495 ± 0.127
1.164HisIle: 1.164 ± 0.17
1.048HisLys: 1.048 ± 0.164
1.31HisLeu: 1.31 ± 0.186
0.553HisMet: 0.553 ± 0.12
0.786HisAsn: 0.786 ± 0.133
0.815HisPro: 0.815 ± 0.174
0.582HisGln: 0.582 ± 0.128
1.513HisArg: 1.513 ± 0.231
1.077HisSer: 1.077 ± 0.18
0.931HisThr: 0.931 ± 0.188
1.251HisVal: 1.251 ± 0.209
0.349HisTrp: 0.349 ± 0.112
0.844HisTyr: 0.844 ± 0.16
0.0HisXaa: 0.0 ± 0.0
Ile
4.86IleAla: 4.86 ± 0.371
0.844IleCys: 0.844 ± 0.18
4.045IleAsp: 4.045 ± 0.355
4.424IleGlu: 4.424 ± 0.364
1.455IlePhe: 1.455 ± 0.184
3.929IleGly: 3.929 ± 0.345
0.873IleHis: 0.873 ± 0.172
2.066IleIle: 2.066 ± 0.27
3.725IleLys: 3.725 ± 0.345
3.58IleLeu: 3.58 ± 0.331
1.251IleMet: 1.251 ± 0.197
2.154IleAsn: 2.154 ± 0.27
2.183IlePro: 2.183 ± 0.244
1.775IleGln: 1.775 ± 0.257
3.492IleArg: 3.492 ± 0.278
3.289IleSer: 3.289 ± 0.285
3.23IleThr: 3.23 ± 0.29
4.133IleVal: 4.133 ± 0.317
0.786IleTrp: 0.786 ± 0.145
1.63IleTyr: 1.63 ± 0.283
0.0IleXaa: 0.0 ± 0.0
Lys
5.733LysAla: 5.733 ± 0.41
0.757LysCys: 0.757 ± 0.169
3.987LysAsp: 3.987 ± 0.433
4.162LysGlu: 4.162 ± 0.435
2.125LysPhe: 2.125 ± 0.231
4.395LysGly: 4.395 ± 0.395
1.251LysHis: 1.251 ± 0.242
3.26LysIle: 3.26 ± 0.325
4.889LysLys: 4.889 ± 0.529
4.191LysLeu: 4.191 ± 0.331
2.445LysMet: 2.445 ± 0.244
3.347LysAsn: 3.347 ± 0.315
2.532LysPro: 2.532 ± 0.324
1.979LysGln: 1.979 ± 0.237
3.754LysArg: 3.754 ± 0.405
3.23LysSer: 3.23 ± 0.338
3.609LysThr: 3.609 ± 0.273
4.569LysVal: 4.569 ± 0.373
1.077LysTrp: 1.077 ± 0.169
2.561LysTyr: 2.561 ± 0.282
0.0LysXaa: 0.0 ± 0.0
Leu
6.956LeuAla: 6.956 ± 0.497
0.873LeuCys: 0.873 ± 0.157
4.627LeuAsp: 4.627 ± 0.338
5.355LeuGlu: 5.355 ± 0.353
2.561LeuPhe: 2.561 ± 0.281
4.86LeuGly: 4.86 ± 0.423
1.281LeuHis: 1.281 ± 0.198
4.22LeuIle: 4.22 ± 0.366
4.424LeuLys: 4.424 ± 0.34
4.598LeuLeu: 4.598 ± 0.399
1.804LeuMet: 1.804 ± 0.231
3.318LeuAsn: 3.318 ± 0.39
2.765LeuPro: 2.765 ± 0.241
1.63LeuGln: 1.63 ± 0.257
4.133LeuArg: 4.133 ± 0.322
5.093LeuSer: 5.093 ± 0.307
5.064LeuThr: 5.064 ± 0.422
4.86LeuVal: 4.86 ± 0.427
1.164LeuTrp: 1.164 ± 0.169
2.416LeuTyr: 2.416 ± 0.26
0.0LeuXaa: 0.0 ± 0.0
Met
2.968MetAla: 2.968 ± 0.277
0.204MetCys: 0.204 ± 0.079
1.368MetAsp: 1.368 ± 0.17
1.659MetGlu: 1.659 ± 0.223
0.698MetPhe: 0.698 ± 0.124
2.066MetGly: 2.066 ± 0.286
0.553MetHis: 0.553 ± 0.129
1.339MetIle: 1.339 ± 0.205
1.863MetLys: 1.863 ± 0.228
2.066MetLeu: 2.066 ± 0.21
0.466MetMet: 0.466 ± 0.116
1.397MetAsn: 1.397 ± 0.202
1.135MetPro: 1.135 ± 0.163
0.989MetGln: 0.989 ± 0.237
2.037MetArg: 2.037 ± 0.244
2.299MetSer: 2.299 ± 0.273
2.27MetThr: 2.27 ± 0.244
1.688MetVal: 1.688 ± 0.22
0.495MetTrp: 0.495 ± 0.114
0.844MetTyr: 0.844 ± 0.163
0.0MetXaa: 0.0 ± 0.0
Asn
3.871AsnAla: 3.871 ± 0.396
0.204AsnCys: 0.204 ± 0.079
2.648AsnAsp: 2.648 ± 0.288
3.26AsnGlu: 3.26 ± 0.344
1.746AsnPhe: 1.746 ± 0.214
4.016AsnGly: 4.016 ± 0.306
1.193AsnHis: 1.193 ± 0.18
2.154AsnIle: 2.154 ± 0.245
2.823AsnLys: 2.823 ± 0.314
3.289AsnLeu: 3.289 ± 0.306
1.251AsnMet: 1.251 ± 0.201
2.212AsnAsn: 2.212 ± 0.309
2.037AsnPro: 2.037 ± 0.249
1.31AsnGln: 1.31 ± 0.208
2.154AsnArg: 2.154 ± 0.249
2.008AsnSer: 2.008 ± 0.282
2.881AsnThr: 2.881 ± 0.389
3.085AsnVal: 3.085 ± 0.378
0.786AsnTrp: 0.786 ± 0.137
1.513AsnTyr: 1.513 ± 0.169
0.0AsnXaa: 0.0 ± 0.0
Pro
2.852ProAla: 2.852 ± 0.266
0.32ProCys: 0.32 ± 0.099
2.998ProAsp: 2.998 ± 0.364
2.91ProGlu: 2.91 ± 0.317
1.601ProPhe: 1.601 ± 0.204
3.347ProGly: 3.347 ± 0.342
0.698ProHis: 0.698 ± 0.125
1.572ProIle: 1.572 ± 0.222
2.037ProLys: 2.037 ± 0.256
2.125ProLeu: 2.125 ± 0.211
0.815ProMet: 0.815 ± 0.126
1.63ProAsn: 1.63 ± 0.231
1.164ProPro: 1.164 ± 0.188
0.873ProGln: 0.873 ± 0.157
2.066ProArg: 2.066 ± 0.251
2.328ProSer: 2.328 ± 0.331
2.561ProThr: 2.561 ± 0.334
3.551ProVal: 3.551 ± 0.38
0.407ProTrp: 0.407 ± 0.131
1.164ProTyr: 1.164 ± 0.215
0.0ProXaa: 0.0 ± 0.0
Gln
2.939GlnAla: 2.939 ± 0.4
0.378GlnCys: 0.378 ± 0.089
1.31GlnAsp: 1.31 ± 0.187
2.532GlnGlu: 2.532 ± 0.287
1.63GlnPhe: 1.63 ± 0.221
1.892GlnGly: 1.892 ± 0.23
0.407GlnHis: 0.407 ± 0.098
1.513GlnIle: 1.513 ± 0.217
2.125GlnLys: 2.125 ± 0.308
2.532GlnLeu: 2.532 ± 0.239
1.077GlnMet: 1.077 ± 0.192
1.281GlnAsn: 1.281 ± 0.193
0.96GlnPro: 0.96 ± 0.178
0.873GlnGln: 0.873 ± 0.255
2.154GlnArg: 2.154 ± 0.297
1.775GlnSer: 1.775 ± 0.253
2.066GlnThr: 2.066 ± 0.303
2.416GlnVal: 2.416 ± 0.266
0.495GlnTrp: 0.495 ± 0.112
1.077GlnTyr: 1.077 ± 0.165
0.0GlnXaa: 0.0 ± 0.0
Arg
4.54ArgAla: 4.54 ± 0.433
0.582ArgCys: 0.582 ± 0.159
3.463ArgAsp: 3.463 ± 0.311
3.667ArgGlu: 3.667 ± 0.372
2.503ArgPhe: 2.503 ± 0.3
4.162ArgGly: 4.162 ± 0.31
1.164ArgHis: 1.164 ± 0.185
2.968ArgIle: 2.968 ± 0.323
3.812ArgLys: 3.812 ± 0.44
3.929ArgLeu: 3.929 ± 0.363
1.717ArgMet: 1.717 ± 0.259
2.939ArgAsn: 2.939 ± 0.264
1.892ArgPro: 1.892 ± 0.221
1.863ArgGln: 1.863 ± 0.29
3.289ArgArg: 3.289 ± 0.432
3.143ArgSer: 3.143 ± 0.253
2.881ArgThr: 2.881 ± 0.302
3.551ArgVal: 3.551 ± 0.309
1.135ArgTrp: 1.135 ± 0.211
2.503ArgTyr: 2.503 ± 0.313
0.0ArgXaa: 0.0 ± 0.0
Ser
4.627SerAla: 4.627 ± 0.516
0.553SerCys: 0.553 ± 0.139
4.307SerAsp: 4.307 ± 0.313
3.9SerGlu: 3.9 ± 0.341
2.27SerPhe: 2.27 ± 0.28
5.966SerGly: 5.966 ± 0.463
0.989SerHis: 0.989 ± 0.172
3.492SerIle: 3.492 ± 0.324
3.9SerLys: 3.9 ± 0.402
4.889SerLeu: 4.889 ± 0.441
1.804SerMet: 1.804 ± 0.23
2.445SerAsn: 2.445 ± 0.294
2.125SerPro: 2.125 ± 0.278
1.804SerGln: 1.804 ± 0.199
2.794SerArg: 2.794 ± 0.253
3.958SerSer: 3.958 ± 0.426
3.26SerThr: 3.26 ± 0.692
4.569SerVal: 4.569 ± 0.451
1.106SerTrp: 1.106 ± 0.183
1.746SerTyr: 1.746 ± 0.293
0.0SerXaa: 0.0 ± 0.0
Thr
5.006ThrAla: 5.006 ± 0.543
0.698ThrCys: 0.698 ± 0.133
3.871ThrAsp: 3.871 ± 0.3
3.638ThrGlu: 3.638 ± 0.338
2.328ThrPhe: 2.328 ± 0.268
5.821ThrGly: 5.821 ± 0.823
0.873ThrHis: 0.873 ± 0.153
3.58ThrIle: 3.58 ± 0.387
3.434ThrLys: 3.434 ± 0.298
4.656ThrLeu: 4.656 ± 0.353
1.019ThrMet: 1.019 ± 0.227
2.794ThrAsn: 2.794 ± 0.425
3.114ThrPro: 3.114 ± 0.419
2.037ThrGln: 2.037 ± 0.245
2.998ThrArg: 2.998 ± 0.331
3.289ThrSer: 3.289 ± 0.325
4.191ThrThr: 4.191 ± 0.728
5.238ThrVal: 5.238 ± 0.513
1.455ThrTrp: 1.455 ± 0.162
2.125ThrTyr: 2.125 ± 0.23
0.0ThrXaa: 0.0 ± 0.0
Val
5.559ValAla: 5.559 ± 0.456
0.728ValCys: 0.728 ± 0.15
4.802ValAsp: 4.802 ± 0.441
5.559ValGlu: 5.559 ± 0.459
2.852ValPhe: 2.852 ± 0.296
4.598ValGly: 4.598 ± 0.341
1.251ValHis: 1.251 ± 0.182
3.958ValIle: 3.958 ± 0.36
4.656ValLys: 4.656 ± 0.472
4.744ValLeu: 4.744 ± 0.401
1.746ValMet: 1.746 ± 0.208
2.474ValAsn: 2.474 ± 0.287
2.998ValPro: 2.998 ± 0.359
2.445ValGln: 2.445 ± 0.292
4.365ValArg: 4.365 ± 0.305
5.151ValSer: 5.151 ± 0.415
4.569ValThr: 4.569 ± 0.547
5.5ValVal: 5.5 ± 0.447
1.368ValTrp: 1.368 ± 0.22
3.172ValTyr: 3.172 ± 0.396
0.0ValXaa: 0.0 ± 0.0
Trp
1.513TrpAla: 1.513 ± 0.202
0.204TrpCys: 0.204 ± 0.081
1.193TrpAsp: 1.193 ± 0.183
1.63TrpGlu: 1.63 ± 0.238
0.611TrpPhe: 0.611 ± 0.132
1.368TrpGly: 1.368 ± 0.195
0.466TrpHis: 0.466 ± 0.137
0.815TrpIle: 0.815 ± 0.146
1.339TrpLys: 1.339 ± 0.252
1.601TrpLeu: 1.601 ± 0.223
0.495TrpMet: 0.495 ± 0.137
0.96TrpAsn: 0.96 ± 0.161
0.495TrpPro: 0.495 ± 0.137
0.698TrpGln: 0.698 ± 0.14
0.698TrpArg: 0.698 ± 0.16
1.164TrpSer: 1.164 ± 0.182
1.251TrpThr: 1.251 ± 0.213
0.902TrpVal: 0.902 ± 0.19
0.437TrpTrp: 0.437 ± 0.116
0.786TrpTyr: 0.786 ± 0.163
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.852TyrAla: 2.852 ± 0.295
0.262TyrCys: 0.262 ± 0.082
2.998TyrAsp: 2.998 ± 0.322
2.794TyrGlu: 2.794 ± 0.327
1.281TyrPhe: 1.281 ± 0.218
3.201TyrGly: 3.201 ± 0.303
0.495TyrHis: 0.495 ± 0.112
1.659TyrIle: 1.659 ± 0.192
1.979TyrLys: 1.979 ± 0.246
3.027TyrLeu: 3.027 ± 0.284
0.873TyrMet: 0.873 ± 0.149
1.63TyrAsn: 1.63 ± 0.224
1.397TyrPro: 1.397 ± 0.247
1.251TyrGln: 1.251 ± 0.169
2.008TyrArg: 2.008 ± 0.253
2.736TyrSer: 2.736 ± 0.292
2.445TyrThr: 2.445 ± 0.237
2.736TyrVal: 2.736 ± 0.364
0.669TyrTrp: 0.669 ± 0.157
1.31TyrTyr: 1.31 ± 0.188
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 209 proteins (34362 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski