Staphylococcus phage phi 11

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Azeredovirinae; Dubowvirus; Staphylococcus virus 11

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q77FU6|Q77FU6_BPPHA Integrase OS=Staphylococcus phage phi 11 OX=2681609 PE=3 SV=1
MM1 pKa = 7.2TFKK4 pKa = 10.98NNHH7 pKa = 5.5NFNEE11 pKa = 4.17LVLTNEE17 pKa = 5.72DD18 pKa = 2.87IRR20 pKa = 11.84ILKK23 pKa = 9.94NVLEE27 pKa = 4.87DD28 pKa = 3.39AVSVYY33 pKa = 10.99DD34 pKa = 4.36EE35 pKa = 4.33YY36 pKa = 11.73SVCNEE41 pKa = 3.92EE42 pKa = 5.59SDD44 pKa = 4.23FAYY47 pKa = 10.74CLLRR51 pKa = 11.84DD52 pKa = 4.65LYY54 pKa = 10.59TLDD57 pKa = 3.74SLAISSNNVV66 pKa = 2.67

Molecular weight: 7.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q8SDV8|Q8SDV8_BPPHA HTH DNA binding protein OS=Staphylococcus phage phi 11 OX=2681609 PE=4 SV=1
MM1 pKa = 7.57SIVKK5 pKa = 10.22INGKK9 pKa = 8.42PYY11 pKa = 10.87KK12 pKa = 9.54FTEE15 pKa = 4.42HH16 pKa = 6.09EE17 pKa = 4.0NEE19 pKa = 4.33LIKK22 pKa = 11.15KK23 pKa = 9.98NGLTPGMVAKK33 pKa = 10.32RR34 pKa = 11.84VRR36 pKa = 11.84GGWALLEE43 pKa = 4.48ALHH46 pKa = 6.68APYY49 pKa = 11.09GMRR52 pKa = 11.84LAEE55 pKa = 4.12YY56 pKa = 10.62KK57 pKa = 10.65EE58 pKa = 4.08IVLSKK63 pKa = 10.44IMEE66 pKa = 4.38RR67 pKa = 11.84EE68 pKa = 4.03SKK70 pKa = 9.36EE71 pKa = 4.1RR72 pKa = 11.84EE73 pKa = 3.7MARR76 pKa = 11.84QRR78 pKa = 11.84RR79 pKa = 11.84KK80 pKa = 9.17EE81 pKa = 3.73AEE83 pKa = 3.86LRR85 pKa = 11.84KK86 pKa = 10.04KK87 pKa = 10.35KK88 pKa = 9.92PHH90 pKa = 6.47LFNVPQKK97 pKa = 10.53HH98 pKa = 6.08SRR100 pKa = 11.84DD101 pKa = 3.97PYY103 pKa = 10.79WFDD106 pKa = 2.95TTYY109 pKa = 11.53NQMFKK114 pKa = 10.07KK115 pKa = 9.13WQEE118 pKa = 3.59AA119 pKa = 3.35

Molecular weight: 14.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

53

0

53

11629

57

910

219.4

25.15

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.718 ± 0.459

0.482 ± 0.092

6.26 ± 0.43

7.868 ± 0.595

4.231 ± 0.243

5.736 ± 0.295

1.66 ± 0.172

7.473 ± 0.35

9.021 ± 0.497

7.653 ± 0.308

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.683 ± 0.146

6.639 ± 0.249

2.889 ± 0.233

3.818 ± 0.191

4.248 ± 0.233

5.572 ± 0.188

6.183 ± 0.258

6.234 ± 0.322

1.195 ± 0.227

4.437 ± 0.361
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski