Solobacterium moorei F0204
Average proteome isoelectric point is 6.27
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2034 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E7MP29|E7MP29_9FIRM NTP_transferase domain-containing protein OS=Solobacterium moorei F0204 OX=706433 GN=HMPREF9430_01301 PE=4 SV=1
MM1 pKa = 7.68 KK2 pKa = 9.98 IYY4 pKa = 10.09 IVHH7 pKa = 6.44 EE8 pKa = 4.29 NGGEE12 pKa = 4.54 YY13 pKa = 10.07 EE14 pKa = 4.64 DD15 pKa = 3.98 EE16 pKa = 3.93 WDD18 pKa = 4.19 NILGAFTTLEE28 pKa = 4.03 KK29 pKa = 10.83 AQEE32 pKa = 3.97 LKK34 pKa = 10.71 DD35 pKa = 3.85 RR36 pKa = 11.84 KK37 pKa = 10.05 EE38 pKa = 4.15 KK39 pKa = 11.19 EE40 pKa = 3.47 NDD42 pKa = 3.13 EE43 pKa = 4.19 YY44 pKa = 11.63 SEE46 pKa = 4.25 KK47 pKa = 10.98 VEE49 pKa = 4.22 LACRR53 pKa = 11.84 VQNEE57 pKa = 4.7 EE58 pKa = 3.65 ITLEE62 pKa = 3.88 QSGLSEE68 pKa = 4.45 EE69 pKa = 4.83 EE70 pKa = 4.07 YY71 pKa = 10.84 EE72 pKa = 5.17 SYY74 pKa = 10.92 CEE76 pKa = 5.08 CDD78 pKa = 3.08 FDD80 pKa = 6.61 DD81 pKa = 3.91 YY82 pKa = 11.75 VNYY85 pKa = 10.76 YY86 pKa = 7.74 ITQITLDD93 pKa = 3.49 KK94 pKa = 10.32 EE95 pKa = 4.35 SRR97 pKa = 11.84 EE98 pKa = 3.87 EE99 pKa = 4.09 SVNLNGASS107 pKa = 3.23
Molecular weight: 12.58 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.864
IPC2_protein 4.113
IPC_protein 4.024
Toseland 3.859
ProMoST 4.126
Dawson 3.961
Bjellqvist 4.113
Wikipedia 3.808
Rodwell 3.859
Grimsley 3.77
Solomon 3.948
Lehninger 3.897
Nozaki 4.075
DTASelect 4.164
Thurlkill 3.872
EMBOSS 3.834
Sillero 4.126
Patrickios 1.888
IPC_peptide 3.961
IPC2_peptide 4.113
IPC2.peptide.svr19 4.022
Protein with the highest isoelectric point:
>tr|E7MLL4|E7MLL4_9FIRM Uncharacterized protein OS=Solobacterium moorei F0204 OX=706433 GN=HMPREF9430_00426 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.38 QPSKK9 pKa = 10.14 KK10 pKa = 9.69 KK11 pKa = 10.67 NKK13 pKa = 7.99 ATHH16 pKa = 6.03 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATVGGRR28 pKa = 11.84 KK29 pKa = 9.25 VINRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.72 GRR39 pKa = 11.84 KK40 pKa = 8.67 VLSAA44 pKa = 4.05
Molecular weight: 5.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.365
IPC2_protein 11.008
IPC_protein 12.325
Toseland 12.501
ProMoST 12.983
Dawson 12.501
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.34
Grimsley 12.544
Solomon 12.983
Lehninger 12.881
Nozaki 12.501
DTASelect 12.486
Thurlkill 12.501
EMBOSS 12.998
Sillero 12.501
Patrickios 12.076
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 8.991
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2034
0
2034
612376
37
2915
301.1
33.98
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.846 ± 0.052
1.251 ± 0.022
5.71 ± 0.049
6.92 ± 0.065
4.317 ± 0.047
6.315 ± 0.053
2.085 ± 0.024
8.309 ± 0.057
7.271 ± 0.05
9.058 ± 0.058
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.994 ± 0.024
4.98 ± 0.039
3.05 ± 0.024
3.484 ± 0.032
3.863 ± 0.037
5.905 ± 0.037
5.687 ± 0.035
6.751 ± 0.048
0.876 ± 0.02
4.33 ± 0.041
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here