Brevibacillus phage Emery

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 102 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S5MU57|S5MU57_9CAUD Uncharacterized protein OS=Brevibacillus phage Emery OX=1296660 GN=EMERY_8 PE=4 SV=1
MM1 pKa = 7.78KK2 pKa = 10.25INTGCSFLYY11 pKa = 10.14PLTGQMIHH19 pKa = 7.24PGQSYY24 pKa = 10.25DD25 pKa = 2.96EE26 pKa = 4.77HH27 pKa = 8.05YY28 pKa = 10.34RR29 pKa = 11.84YY30 pKa = 9.42PDD32 pKa = 2.99QDD34 pKa = 3.17EE35 pKa = 4.32SVEE38 pKa = 4.25VIEE41 pKa = 5.99DD42 pKa = 3.39EE43 pKa = 4.84TEE45 pKa = 3.85FATEE49 pKa = 4.11GTPEE53 pKa = 3.93EE54 pKa = 4.49VVAQEE59 pKa = 4.18FLTLEE64 pKa = 4.18EE65 pKa = 4.1FDD67 pKa = 3.84KK68 pKa = 11.8LEE70 pKa = 4.0AVKK73 pKa = 10.59QKK75 pKa = 10.65EE76 pKa = 4.26VLVGNLIVSAEE87 pKa = 4.1DD88 pKa = 3.35ADD90 pKa = 3.92AVSNKK95 pKa = 8.18EE96 pKa = 4.72KK97 pKa = 10.72RR98 pKa = 11.84LQLYY102 pKa = 9.69KK103 pKa = 10.72QFLEE107 pKa = 4.63ANAHH111 pKa = 6.49DD112 pKa = 4.4GAGQEE117 pKa = 4.21VTLL120 pKa = 4.65

Molecular weight: 13.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U5P4N0|U5P4N0_9CAUD Uncharacterized protein OS=Brevibacillus phage Emery OX=1296660 GN=EMERY_45 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 10.43SEE4 pKa = 3.71NRR6 pKa = 11.84KK7 pKa = 9.19PYY9 pKa = 9.89NINVSSTVRR18 pKa = 11.84IFMKK22 pKa = 10.63VEE24 pKa = 3.61NNQVRR29 pKa = 11.84DD30 pKa = 3.49GRR32 pKa = 11.84KK33 pKa = 9.12YY34 pKa = 10.77RR35 pKa = 11.84ITRR38 pKa = 11.84CYY40 pKa = 10.24QGFLASEE47 pKa = 4.01LRR49 pKa = 11.84FVQNLSKK56 pKa = 10.41MLL58 pKa = 4.03

Molecular weight: 6.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

102

0

102

18708

16

1006

183.4

20.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.954 ± 0.365

0.823 ± 0.101

5.623 ± 0.232

7.489 ± 0.273

4.068 ± 0.159

6.35 ± 0.27

1.684 ± 0.101

6.815 ± 0.258

7.735 ± 0.245

9.253 ± 0.315

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.63 ± 0.12

4.356 ± 0.152

3.667 ± 0.189

4.004 ± 0.178

5.297 ± 0.207

6.527 ± 0.208

5.361 ± 0.178

6.5 ± 0.19

1.363 ± 0.103

3.501 ± 0.191
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski