Amino acid dipepetide frequency for Lysinibacillus sp. BF-4

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.696AlaAla: 7.696 ± 0.136
0.644AlaCys: 0.644 ± 0.031
4.378AlaAsp: 4.378 ± 0.086
5.649AlaGlu: 5.649 ± 0.103
3.827AlaPhe: 3.827 ± 0.08
5.757AlaGly: 5.757 ± 0.11
1.802AlaHis: 1.802 ± 0.049
7.028AlaIle: 7.028 ± 0.107
5.785AlaLys: 5.785 ± 0.091
9.817AlaLeu: 9.817 ± 0.139
3.02AlaMet: 3.02 ± 0.076
3.242AlaAsn: 3.242 ± 0.068
2.813AlaPro: 2.813 ± 0.072
3.946AlaGln: 3.946 ± 0.077
3.63AlaArg: 3.63 ± 0.078
4.683AlaSer: 4.683 ± 0.092
6.022AlaThr: 6.022 ± 0.1
6.662AlaVal: 6.662 ± 0.106
0.693AlaTrp: 0.693 ± 0.029
2.959AlaTyr: 2.959 ± 0.068
0.0AlaXaa: 0.0 ± 0.0
Cys
0.503CysAla: 0.503 ± 0.027
0.072CysCys: 0.072 ± 0.011
0.358CysAsp: 0.358 ± 0.025
0.481CysGlu: 0.481 ± 0.025
0.227CysPhe: 0.227 ± 0.019
0.649CysGly: 0.649 ± 0.034
0.167CysHis: 0.167 ± 0.017
0.442CysIle: 0.442 ± 0.023
0.271CysLys: 0.271 ± 0.019
0.547CysLeu: 0.547 ± 0.03
0.137CysMet: 0.137 ± 0.014
0.247CysAsn: 0.247 ± 0.019
0.306CysPro: 0.306 ± 0.022
0.25CysGln: 0.25 ± 0.021
0.234CysArg: 0.234 ± 0.017
0.391CysSer: 0.391 ± 0.025
0.47CysThr: 0.47 ± 0.027
0.449CysVal: 0.449 ± 0.026
0.048CysTrp: 0.048 ± 0.009
0.244CysTyr: 0.244 ± 0.021
0.0CysXaa: 0.0 ± 0.0
Asp
4.609AspAla: 4.609 ± 0.08
0.331AspCys: 0.331 ± 0.022
2.694AspAsp: 2.694 ± 0.073
4.742AspGlu: 4.742 ± 0.087
2.567AspPhe: 2.567 ± 0.064
3.576AspGly: 3.576 ± 0.082
0.837AspHis: 0.837 ± 0.041
3.908AspIle: 3.908 ± 0.08
3.242AspLys: 3.242 ± 0.081
4.449AspLeu: 4.449 ± 0.08
1.436AspMet: 1.436 ± 0.041
1.908AspAsn: 1.908 ± 0.058
1.535AspPro: 1.535 ± 0.048
1.371AspGln: 1.371 ± 0.045
1.845AspArg: 1.845 ± 0.056
2.157AspSer: 2.157 ± 0.057
2.817AspThr: 2.817 ± 0.063
4.509AspVal: 4.509 ± 0.087
0.566AspTrp: 0.566 ± 0.029
2.148AspTyr: 2.148 ± 0.057
0.0AspXaa: 0.0 ± 0.0
Glu
7.018GluAla: 7.018 ± 0.11
0.304GluCys: 0.304 ± 0.022
3.768GluAsp: 3.768 ± 0.08
5.86GluGlu: 5.86 ± 0.119
1.987GluPhe: 1.987 ± 0.05
4.338GluGly: 4.338 ± 0.069
1.646GluHis: 1.646 ± 0.048
4.563GluIle: 4.563 ± 0.086
4.508GluLys: 4.508 ± 0.096
6.847GluLeu: 6.847 ± 0.095
2.186GluMet: 2.186 ± 0.057
2.503GluAsn: 2.503 ± 0.069
2.018GluPro: 2.018 ± 0.058
4.643GluGln: 4.643 ± 0.11
4.026GluArg: 4.026 ± 0.081
2.556GluSer: 2.556 ± 0.056
3.427GluThr: 3.427 ± 0.066
5.582GluVal: 5.582 ± 0.091
0.67GluTrp: 0.67 ± 0.028
1.673GluTyr: 1.673 ± 0.044
0.0GluXaa: 0.0 ± 0.0
Phe
3.752PheAla: 3.752 ± 0.08
0.272PheCys: 0.272 ± 0.019
2.396PheAsp: 2.396 ± 0.059
2.806PheGlu: 2.806 ± 0.051
2.106PhePhe: 2.106 ± 0.06
3.141PheGly: 3.141 ± 0.069
0.771PheHis: 0.771 ± 0.032
3.561PheIle: 3.561 ± 0.082
2.162PheLys: 2.162 ± 0.057
3.797PheLeu: 3.797 ± 0.083
1.265PheMet: 1.265 ± 0.042
1.673PheAsn: 1.673 ± 0.052
1.399PhePro: 1.399 ± 0.051
1.157PheGln: 1.157 ± 0.039
1.353PheArg: 1.353 ± 0.047
2.602PheSer: 2.602 ± 0.057
3.194PheThr: 3.194 ± 0.069
3.414PheVal: 3.414 ± 0.073
0.404PheTrp: 0.404 ± 0.025
1.628PheTyr: 1.628 ± 0.046
0.0PheXaa: 0.0 ± 0.0
Gly
5.738GlyAla: 5.738 ± 0.118
0.53GlyCys: 0.53 ± 0.029
3.371GlyAsp: 3.371 ± 0.077
4.585GlyGlu: 4.585 ± 0.091
3.172GlyPhe: 3.172 ± 0.068
4.579GlyGly: 4.579 ± 0.126
1.343GlyHis: 1.343 ± 0.045
5.229GlyIle: 5.229 ± 0.093
4.42GlyLys: 4.42 ± 0.08
6.199GlyLeu: 6.199 ± 0.099
2.158GlyMet: 2.158 ± 0.059
2.293GlyAsn: 2.293 ± 0.062
1.533GlyPro: 1.533 ± 0.045
2.242GlyGln: 2.242 ± 0.066
2.625GlyArg: 2.625 ± 0.068
3.18GlySer: 3.18 ± 0.077
4.045GlyThr: 4.045 ± 0.085
5.408GlyVal: 5.408 ± 0.095
0.637GlyTrp: 0.637 ± 0.028
2.657GlyTyr: 2.657 ± 0.067
0.0GlyXaa: 0.0 ± 0.0
His
1.681HisAla: 1.681 ± 0.05
0.181HisCys: 0.181 ± 0.015
1.087HisAsp: 1.087 ± 0.039
1.444HisGlu: 1.444 ± 0.043
1.007HisPhe: 1.007 ± 0.039
1.345HisGly: 1.345 ± 0.045
0.583HisHis: 0.583 ± 0.03
1.643HisIle: 1.643 ± 0.047
1.109HisLys: 1.109 ± 0.038
1.976HisLeu: 1.976 ± 0.052
0.527HisMet: 0.527 ± 0.024
0.83HisAsn: 0.83 ± 0.035
1.036HisPro: 1.036 ± 0.038
0.805HisGln: 0.805 ± 0.034
0.862HisArg: 0.862 ± 0.036
1.038HisSer: 1.038 ± 0.038
1.283HisThr: 1.283 ± 0.048
1.555HisVal: 1.555 ± 0.053
0.171HisTrp: 0.171 ± 0.014
1.056HisTyr: 1.056 ± 0.04
0.0HisXaa: 0.0 ± 0.0
Ile
7.605IleAla: 7.605 ± 0.118
0.503IleCys: 0.503 ± 0.028
3.963IleAsp: 3.963 ± 0.078
5.527IleGlu: 5.527 ± 0.08
2.954IlePhe: 2.954 ± 0.071
5.478IleGly: 5.478 ± 0.101
1.324IleHis: 1.324 ± 0.044
5.543IleIle: 5.543 ± 0.11
3.667IleLys: 3.667 ± 0.076
5.757IleLeu: 5.757 ± 0.099
1.957IleMet: 1.957 ± 0.055
2.553IleAsn: 2.553 ± 0.072
2.602IlePro: 2.602 ± 0.067
2.392IleGln: 2.392 ± 0.054
2.762IleArg: 2.762 ± 0.064
3.853IleSer: 3.853 ± 0.078
5.224IleThr: 5.224 ± 0.087
5.937IleVal: 5.937 ± 0.093
0.529IleTrp: 0.529 ± 0.027
2.207IleTyr: 2.207 ± 0.06
0.0IleXaa: 0.0 ± 0.0
Lys
4.859LysAla: 4.859 ± 0.102
0.294LysCys: 0.294 ± 0.022
3.339LysAsp: 3.339 ± 0.07
5.126LysGlu: 5.126 ± 0.096
1.831LysPhe: 1.831 ± 0.055
3.627LysGly: 3.627 ± 0.071
1.332LysHis: 1.332 ± 0.04
3.723LysIle: 3.723 ± 0.068
4.369LysLys: 4.369 ± 0.089
5.365LysLeu: 5.365 ± 0.085
2.134LysMet: 2.134 ± 0.057
2.497LysAsn: 2.497 ± 0.068
2.197LysPro: 2.197 ± 0.06
3.182LysGln: 3.182 ± 0.071
3.093LysArg: 3.093 ± 0.072
2.643LysSer: 2.643 ± 0.069
3.456LysThr: 3.456 ± 0.073
4.163LysVal: 4.163 ± 0.078
0.662LysTrp: 0.662 ± 0.028
1.856LysTyr: 1.856 ± 0.054
0.0LysXaa: 0.0 ± 0.0
Leu
9.885LeuAla: 9.885 ± 0.146
0.618LeuCys: 0.618 ± 0.028
4.585LeuAsp: 4.585 ± 0.083
5.525LeuGlu: 5.525 ± 0.094
4.219LeuPhe: 4.219 ± 0.099
6.342LeuGly: 6.342 ± 0.113
2.175LeuHis: 2.175 ± 0.056
6.21LeuIle: 6.21 ± 0.114
4.854LeuLys: 4.854 ± 0.089
10.125LeuLeu: 10.125 ± 0.143
2.563LeuMet: 2.563 ± 0.061
3.448LeuAsn: 3.448 ± 0.074
4.293LeuPro: 4.293 ± 0.081
4.877LeuGln: 4.877 ± 0.106
4.303LeuArg: 4.303 ± 0.086
5.324LeuSer: 5.324 ± 0.077
6.751LeuThr: 6.751 ± 0.092
6.974LeuVal: 6.974 ± 0.102
0.77LeuTrp: 0.77 ± 0.036
3.057LeuTyr: 3.057 ± 0.079
0.0LeuXaa: 0.0 ± 0.0
Met
2.426MetAla: 2.426 ± 0.059
0.168MetCys: 0.168 ± 0.016
1.467MetAsp: 1.467 ± 0.048
1.62MetGlu: 1.62 ± 0.044
1.073MetPhe: 1.073 ± 0.041
1.719MetGly: 1.719 ± 0.051
0.61MetHis: 0.61 ± 0.031
2.016MetIle: 2.016 ± 0.046
2.26MetLys: 2.26 ± 0.056
3.057MetLeu: 3.057 ± 0.059
0.958MetMet: 0.958 ± 0.039
1.404MetAsn: 1.404 ± 0.043
1.266MetPro: 1.266 ± 0.04
1.45MetGln: 1.45 ± 0.044
1.467MetArg: 1.467 ± 0.045
1.634MetSer: 1.634 ± 0.043
2.222MetThr: 2.222 ± 0.058
1.81MetVal: 1.81 ± 0.05
0.181MetTrp: 0.181 ± 0.016
0.881MetTyr: 0.881 ± 0.033
0.0MetXaa: 0.0 ± 0.0
Asn
3.18AsnAla: 3.18 ± 0.082
0.258AsnCys: 0.258 ± 0.019
2.046AsnAsp: 2.046 ± 0.062
3.263AsnGlu: 3.263 ± 0.068
1.662AsnPhe: 1.662 ± 0.051
2.549AsnGly: 2.549 ± 0.063
0.806AsnHis: 0.806 ± 0.034
2.745AsnIle: 2.745 ± 0.069
2.309AsnLys: 2.309 ± 0.059
3.026AsnLeu: 3.026 ± 0.064
1.077AsnMet: 1.077 ± 0.041
1.443AsnAsn: 1.443 ± 0.055
1.72AsnPro: 1.72 ± 0.053
1.39AsnGln: 1.39 ± 0.045
1.753AsnArg: 1.753 ± 0.055
1.537AsnSer: 1.537 ± 0.049
2.131AsnThr: 2.131 ± 0.054
2.9AsnVal: 2.9 ± 0.069
0.376AsnTrp: 0.376 ± 0.026
1.416AsnTyr: 1.416 ± 0.046
0.0AsnXaa: 0.0 ± 0.0
Pro
2.83ProAla: 2.83 ± 0.068
0.196ProCys: 0.196 ± 0.016
1.59ProAsp: 1.59 ± 0.042
2.452ProGlu: 2.452 ± 0.065
1.775ProPhe: 1.775 ± 0.052
1.927ProGly: 1.927 ± 0.055
0.893ProHis: 0.893 ± 0.034
2.597ProIle: 2.597 ± 0.066
2.273ProLys: 2.273 ± 0.065
3.497ProLeu: 3.497 ± 0.092
1.001ProMet: 1.001 ± 0.035
1.503ProAsn: 1.503 ± 0.045
0.944ProPro: 0.944 ± 0.039
1.44ProGln: 1.44 ± 0.048
1.231ProArg: 1.231 ± 0.041
2.085ProSer: 2.085 ± 0.06
2.687ProThr: 2.687 ± 0.067
2.909ProVal: 2.909 ± 0.062
0.307ProTrp: 0.307 ± 0.022
1.331ProTyr: 1.331 ± 0.046
0.0ProXaa: 0.0 ± 0.0
Gln
4.348GlnAla: 4.348 ± 0.097
0.182GlnCys: 0.182 ± 0.018
2.004GlnAsp: 2.004 ± 0.055
2.771GlnGlu: 2.771 ± 0.074
1.67GlnPhe: 1.67 ± 0.053
2.527GlnGly: 2.527 ± 0.056
1.108GlnHis: 1.108 ± 0.042
2.492GlnIle: 2.492 ± 0.064
2.422GlnLys: 2.422 ± 0.057
4.94GlnLeu: 4.94 ± 0.097
1.342GlnMet: 1.342 ± 0.049
1.451GlnAsn: 1.451 ± 0.044
1.509GlnPro: 1.509 ± 0.053
3.316GlnGln: 3.316 ± 0.112
1.971GlnArg: 1.971 ± 0.06
2.075GlnSer: 2.075 ± 0.066
2.361GlnThr: 2.361 ± 0.069
2.936GlnVal: 2.936 ± 0.064
0.391GlnTrp: 0.391 ± 0.027
1.318GlnTyr: 1.318 ± 0.041
0.0GlnXaa: 0.0 ± 0.0
Arg
3.442ArgAla: 3.442 ± 0.077
0.272ArgCys: 0.272 ± 0.02
2.315ArgAsp: 2.315 ± 0.053
3.318ArgGlu: 3.318 ± 0.08
1.938ArgPhe: 1.938 ± 0.053
2.507ArgGly: 2.507 ± 0.065
0.858ArgHis: 0.858 ± 0.032
3.106ArgIle: 3.106 ± 0.065
2.929ArgLys: 2.929 ± 0.066
4.114ArgLeu: 4.114 ± 0.088
1.401ArgMet: 1.401 ± 0.049
1.681ArgAsn: 1.681 ± 0.054
1.392ArgPro: 1.392 ± 0.045
1.84ArgGln: 1.84 ± 0.055
1.899ArgArg: 1.899 ± 0.059
2.011ArgSer: 2.011 ± 0.05
2.403ArgThr: 2.403 ± 0.058
3.137ArgVal: 3.137 ± 0.073
0.346ArgTrp: 0.346 ± 0.021
1.72ArgTyr: 1.72 ± 0.052
0.0ArgXaa: 0.0 ± 0.0
Ser
4.005SerAla: 4.005 ± 0.081
0.359SerCys: 0.359 ± 0.026
2.377SerAsp: 2.377 ± 0.055
3.121SerGlu: 3.121 ± 0.078
2.74SerPhe: 2.74 ± 0.062
3.654SerGly: 3.654 ± 0.08
1.029SerHis: 1.029 ± 0.035
3.867SerIle: 3.867 ± 0.069
2.698SerLys: 2.698 ± 0.066
5.136SerLeu: 5.136 ± 0.101
1.433SerMet: 1.433 ± 0.048
1.828SerAsn: 1.828 ± 0.051
1.885SerPro: 1.885 ± 0.051
1.674SerGln: 1.674 ± 0.054
1.967SerArg: 1.967 ± 0.047
2.935SerSer: 2.935 ± 0.074
3.153SerThr: 3.153 ± 0.066
3.867SerVal: 3.867 ± 0.068
0.464SerTrp: 0.464 ± 0.026
2.176SerTyr: 2.176 ± 0.063
0.0SerXaa: 0.0 ± 0.0
Thr
5.638ThrAla: 5.638 ± 0.108
0.415ThrCys: 0.415 ± 0.024
2.929ThrAsp: 2.929 ± 0.063
3.869ThrGlu: 3.869 ± 0.079
2.841ThrPhe: 2.841 ± 0.06
4.268ThrGly: 4.268 ± 0.086
1.411ThrHis: 1.411 ± 0.041
5.132ThrIle: 5.132 ± 0.099
3.978ThrLys: 3.978 ± 0.088
6.491ThrLeu: 6.491 ± 0.096
1.715ThrMet: 1.715 ± 0.049
2.499ThrAsn: 2.499 ± 0.053
2.695ThrPro: 2.695 ± 0.064
2.178ThrGln: 2.178 ± 0.058
2.307ThrArg: 2.307 ± 0.053
3.389ThrSer: 3.389 ± 0.074
4.694ThrThr: 4.694 ± 0.094
5.094ThrVal: 5.094 ± 0.099
0.527ThrTrp: 0.527 ± 0.028
2.284ThrTyr: 2.284 ± 0.06
0.0ThrXaa: 0.0 ± 0.0
Val
7.413ValAla: 7.413 ± 0.105
0.555ValCys: 0.555 ± 0.028
3.902ValAsp: 3.902 ± 0.08
4.915ValGlu: 4.915 ± 0.083
3.109ValPhe: 3.109 ± 0.07
5.041ValGly: 5.041 ± 0.107
1.492ValHis: 1.492 ± 0.042
5.655ValIle: 5.655 ± 0.092
4.334ValLys: 4.334 ± 0.082
7.436ValLeu: 7.436 ± 0.118
2.179ValMet: 2.179 ± 0.054
2.862ValAsn: 2.862 ± 0.062
2.891ValPro: 2.891 ± 0.073
2.921ValGln: 2.921 ± 0.076
3.214ValArg: 3.214 ± 0.075
4.08ValSer: 4.08 ± 0.081
5.433ValThr: 5.433 ± 0.099
6.168ValVal: 6.168 ± 0.11
0.63ValTrp: 0.63 ± 0.028
2.231ValTyr: 2.231 ± 0.057
0.0ValXaa: 0.0 ± 0.0
Trp
0.585TrpAla: 0.585 ± 0.028
0.064TrpCys: 0.064 ± 0.011
0.481TrpAsp: 0.481 ± 0.028
0.478TrpGlu: 0.478 ± 0.026
0.411TrpPhe: 0.411 ± 0.027
0.531TrpGly: 0.531 ± 0.03
0.297TrpHis: 0.297 ± 0.021
0.541TrpIle: 0.541 ± 0.03
0.388TrpLys: 0.388 ± 0.026
1.255TrpLeu: 1.255 ± 0.05
0.247TrpMet: 0.247 ± 0.019
0.28TrpAsn: 0.28 ± 0.023
0.198TrpPro: 0.198 ± 0.019
0.669TrpGln: 0.669 ± 0.037
0.442TrpArg: 0.442 ± 0.027
0.501TrpSer: 0.501 ± 0.027
0.402TrpThr: 0.402 ± 0.024
0.588TrpVal: 0.588 ± 0.026
0.116TrpTrp: 0.116 ± 0.012
0.322TrpTyr: 0.322 ± 0.023
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.839TyrAla: 2.839 ± 0.068
0.271TyrCys: 0.271 ± 0.019
2.201TyrAsp: 2.201 ± 0.066
2.57TyrGlu: 2.57 ± 0.063
1.74TyrPhe: 1.74 ± 0.05
2.386TyrGly: 2.386 ± 0.064
0.735TyrHis: 0.735 ± 0.034
2.339TyrIle: 2.339 ± 0.059
1.771TyrLys: 1.771 ± 0.056
3.071TyrLeu: 3.071 ± 0.064
0.949TyrMet: 0.949 ± 0.035
1.441TyrAsn: 1.441 ± 0.044
1.235TyrPro: 1.235 ± 0.042
1.394TyrGln: 1.394 ± 0.048
1.645TyrArg: 1.645 ± 0.048
1.695TyrSer: 1.695 ± 0.05
2.173TyrThr: 2.173 ± 0.052
2.398TyrVal: 2.398 ± 0.064
0.31TyrTrp: 0.31 ± 0.026
1.441TyrTyr: 1.441 ± 0.05
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2407 proteins (713184 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski