Capra hircus papillomavirus 1
Average proteome isoelectric point is 6.31
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q1I123|Q1I123_9PAPI E4 OS=Capra hircus papillomavirus 1 OX=338903 GN=E4 PE=4 SV=1
MM1 pKa = 7.01 VRR3 pKa = 11.84 AKK5 pKa = 9.95 RR6 pKa = 11.84 VKK8 pKa = 10.1 RR9 pKa = 11.84 ASEE12 pKa = 3.8 DD13 pKa = 3.2 QLYY16 pKa = 10.14 RR17 pKa = 11.84 DD18 pKa = 4.04 CLAGRR23 pKa = 11.84 EE24 pKa = 4.31 CKK26 pKa = 9.91 PDD28 pKa = 3.22 IKK30 pKa = 10.85 DD31 pKa = 3.2 KK32 pKa = 11.64 YY33 pKa = 9.86 EE34 pKa = 4.15 NNTLADD40 pKa = 5.03 SILKK44 pKa = 8.89 WASQFLWFGQLGIGTGRR61 pKa = 11.84 GSGGSGGYY69 pKa = 7.9 TRR71 pKa = 11.84 LGGGGGGSRR80 pKa = 11.84 TGVISRR86 pKa = 11.84 PPVVIDD92 pKa = 3.76 SIGPRR97 pKa = 11.84 DD98 pKa = 3.79 ITPIDD103 pKa = 3.83 TVDD106 pKa = 3.55 PTASSIVPLNEE117 pKa = 4.34 GGPSVPTGEE126 pKa = 4.1 IEE128 pKa = 4.75 VIAEE132 pKa = 4.26 TTPTTSNGALDD143 pKa = 3.87 PVIVGEE149 pKa = 4.43 PDD151 pKa = 3.4 LGPATIDD158 pKa = 3.38 VSSDD162 pKa = 3.3 PTPSNPVLNTTVSSSIHH179 pKa = 5.99 NNPSFDD185 pKa = 3.71 AAILSSQLPGEE196 pKa = 4.29 FSVPDD201 pKa = 3.43 QVVITHH207 pKa = 6.47 GLVGEE212 pKa = 4.63 HH213 pKa = 6.44 IGAFEE218 pKa = 4.59 NIEE221 pKa = 4.28 LDD223 pKa = 3.8 TIGEE227 pKa = 4.01 YY228 pKa = 10.59 SGFGEE233 pKa = 4.91 LEE235 pKa = 4.06 VEE237 pKa = 4.44 EE238 pKa = 5.3 PPSSSTPQTGLSARR252 pKa = 11.84 LSKK255 pKa = 10.57 NLKK258 pKa = 7.75 QLRR261 pKa = 11.84 QRR263 pKa = 11.84 LYY265 pKa = 10.6 NRR267 pKa = 11.84 RR268 pKa = 11.84 IQQRR272 pKa = 11.84 LVEE275 pKa = 4.28 NQPFLTSPRR284 pKa = 11.84 RR285 pKa = 11.84 LVQFEE290 pKa = 4.12 YY291 pKa = 10.96 SNPSFQDD298 pKa = 3.3 EE299 pKa = 4.35 VSLEE303 pKa = 3.9 FEE305 pKa = 4.01 RR306 pKa = 11.84 DD307 pKa = 2.96 INTVVEE313 pKa = 4.28 APHH316 pKa = 6.59 YY317 pKa = 10.21 DD318 pKa = 3.08 FRR320 pKa = 11.84 DD321 pKa = 3.8 VVRR324 pKa = 11.84 LGRR327 pKa = 11.84 PEE329 pKa = 4.86 SNLTDD334 pKa = 3.6 SGHH337 pKa = 5.46 VRR339 pKa = 11.84 VSRR342 pKa = 11.84 LGTLGTISTRR352 pKa = 11.84 SGLIIGGQVHH362 pKa = 6.85 YY363 pKa = 9.64 YY364 pKa = 9.88 TDD366 pKa = 4.07 LSSIAADD373 pKa = 3.93 TIEE376 pKa = 4.45 LPSYY380 pKa = 9.52 QPAMSPSTIVHH391 pKa = 6.54 IDD393 pKa = 3.27 SAGEE397 pKa = 4.01 TFNDD401 pKa = 4.02 MEE403 pKa = 4.55 TSFCNVDD410 pKa = 3.16 QGIEE414 pKa = 4.07 EE415 pKa = 4.31 VEE417 pKa = 3.92 RR418 pKa = 11.84 DD419 pKa = 3.84 LLDD422 pKa = 3.76 SASEE426 pKa = 4.09 DD427 pKa = 3.75 FSNSRR432 pKa = 11.84 LLLSTGSDD440 pKa = 3.11 IVDD443 pKa = 3.75 SGLDD447 pKa = 3.42 LQDD450 pKa = 2.75 IFQRR454 pKa = 11.84 PNLGEE459 pKa = 4.2 DD460 pKa = 3.62 SITVNFPTSNSIQVFNGSGAGSSIPATPIGPLGPVPIPAVLFNVLSSDD508 pKa = 4.16 LLLDD512 pKa = 3.9 PSLLRR517 pKa = 11.84 KK518 pKa = 9.25 RR519 pKa = 11.84 RR520 pKa = 11.84 KK521 pKa = 9.72 KK522 pKa = 10.88 YY523 pKa = 10.75 GVFSS527 pKa = 3.89
Molecular weight: 56.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.67
IPC2_protein 4.596
IPC_protein 4.558
Toseland 4.393
ProMoST 4.673
Dawson 4.52
Bjellqvist 4.66
Wikipedia 4.393
Rodwell 4.393
Grimsley 4.304
Solomon 4.507
Lehninger 4.469
Nozaki 4.622
DTASelect 4.813
Thurlkill 4.406
EMBOSS 4.418
Sillero 4.673
Patrickios 4.062
IPC_peptide 4.52
IPC2_peptide 4.66
IPC2.peptide.svr19 4.641
Protein with the highest isoelectric point:
>tr|Q1I125|Q1I125_9PAPI Replication protein E1 OS=Capra hircus papillomavirus 1 OX=338903 GN=E1 PE=3 SV=1
MM1 pKa = 6.99 TALEE5 pKa = 3.99 ARR7 pKa = 11.84 FEE9 pKa = 4.16 EE10 pKa = 4.62 VQEE13 pKa = 4.16 KK14 pKa = 10.5 LLEE17 pKa = 4.22 LLEE20 pKa = 4.8 RR21 pKa = 11.84 NPEE24 pKa = 4.06 TLDD27 pKa = 3.32 EE28 pKa = 4.3 QIEE31 pKa = 4.24 YY32 pKa = 9.94 WEE34 pKa = 4.07 LVKK37 pKa = 10.44 WEE39 pKa = 4.3 NIYY42 pKa = 11.22 YY43 pKa = 10.05 NAARR47 pKa = 11.84 RR48 pKa = 11.84 KK49 pKa = 9.57 GIHH52 pKa = 6.58 KK53 pKa = 10.34 LGFSPVPSLQASEE66 pKa = 5.04 HH67 pKa = 5.68 GAKK70 pKa = 9.78 EE71 pKa = 4.05 AIGMKK76 pKa = 10.26 LQLTNLKK83 pKa = 10.47 SSGFGSEE90 pKa = 3.62 KK91 pKa = 8.64 WTLADD96 pKa = 3.8 TSSMTYY102 pKa = 10.38 LAPPKK107 pKa = 9.59 HH108 pKa = 5.23 TFKK111 pKa = 11.12 KK112 pKa = 9.76 LSKK115 pKa = 9.27 PVTVTFDD122 pKa = 3.87 SNSQNAVRR130 pKa = 11.84 YY131 pKa = 6.2 TCWSHH136 pKa = 7.28 VYY138 pKa = 10.13 YY139 pKa = 11.17 LNDD142 pKa = 2.84 NDD144 pKa = 4.79 MYY146 pKa = 10.95 VKK148 pKa = 10.32 SYY150 pKa = 11.49 SNVDD154 pKa = 3.11 VHH156 pKa = 5.9 GVYY159 pKa = 10.74 YY160 pKa = 10.3 IRR162 pKa = 11.84 DD163 pKa = 3.5 SRR165 pKa = 11.84 KK166 pKa = 8.14 QYY168 pKa = 10.47 YY169 pKa = 10.79 VNFEE173 pKa = 3.65 KK174 pKa = 10.76 DD175 pKa = 2.96 ARR177 pKa = 11.84 KK178 pKa = 10.03 YY179 pKa = 11.04 SSTGQWDD186 pKa = 3.46 VSFEE190 pKa = 4.12 NKK192 pKa = 9.75 HH193 pKa = 5.81 LSSSIASSSGHH204 pKa = 5.46 TATVATPEE212 pKa = 4.25 TTVTSTQASAAAASAADD229 pKa = 4.03 SQEE232 pKa = 4.01 EE233 pKa = 4.89 TPGSTRR239 pKa = 11.84 KK240 pKa = 10.01 LLTATYY246 pKa = 10.43 HH247 pKa = 6.77 RR248 pKa = 11.84 ILRR251 pKa = 11.84 DD252 pKa = 3.23 RR253 pKa = 11.84 GSPRR257 pKa = 11.84 PASRR261 pKa = 11.84 SRR263 pKa = 11.84 SRR265 pKa = 11.84 SSTSTSSSGSSYY277 pKa = 10.66 RR278 pKa = 11.84 PGSRR282 pKa = 11.84 RR283 pKa = 11.84 SSSGRR288 pKa = 11.84 GARR291 pKa = 11.84 RR292 pKa = 11.84 SRR294 pKa = 11.84 GRR296 pKa = 11.84 SWRR299 pKa = 11.84 RR300 pKa = 11.84 TRR302 pKa = 11.84 SRR304 pKa = 11.84 SQSTEE309 pKa = 3.25 RR310 pKa = 11.84 SRR312 pKa = 11.84 GGGGGRR318 pKa = 11.84 GRR320 pKa = 11.84 GRR322 pKa = 11.84 PRR324 pKa = 11.84 RR325 pKa = 11.84 SGLHH329 pKa = 5.38 SRR331 pKa = 11.84 PSRR334 pKa = 11.84 SPAPKK339 pKa = 10.08 GSGVPKK345 pKa = 10.04 RR346 pKa = 11.84 RR347 pKa = 11.84 PSVRR351 pKa = 11.84 APQSYY356 pKa = 10.24 RR357 pKa = 11.84 GGSQQGTPPRR367 pKa = 11.84 PTAVSVASATPDD379 pKa = 3.14 IPRR382 pKa = 11.84 LSPWFRR388 pKa = 11.84 RR389 pKa = 11.84 RR390 pKa = 11.84 SHH392 pKa = 5.32 STPRR396 pKa = 11.84 SPVRR400 pKa = 11.84 APGRR404 pKa = 11.84 GSPPASLGSVRR415 pKa = 11.84 HH416 pKa = 5.66 HH417 pKa = 6.54 QSSQRR422 pKa = 11.84 KK423 pKa = 8.09 RR424 pKa = 11.84 SRR426 pKa = 11.84 SHH428 pKa = 6.09 EE429 pKa = 4.07 RR430 pKa = 11.84 LHH432 pKa = 6.79 SAVKK436 pKa = 9.95 KK437 pKa = 10.04 DD438 pKa = 3.4 PYY440 pKa = 10.57 VILVRR445 pKa = 11.84 GKK447 pKa = 9.58 PNQLKK452 pKa = 8.33 CWRR455 pKa = 11.84 WRR457 pKa = 11.84 LHH459 pKa = 4.34 NRR461 pKa = 11.84 ARR463 pKa = 11.84 RR464 pKa = 11.84 VPFRR468 pKa = 11.84 CFSTTFSWVSLVQGEE483 pKa = 4.15 KK484 pKa = 10.53 SIANRR489 pKa = 11.84 MLVVFDD495 pKa = 3.63 TRR497 pKa = 11.84 QDD499 pKa = 3.33 RR500 pKa = 11.84 EE501 pKa = 4.36 LFLHH505 pKa = 6.8 RR506 pKa = 11.84 VTFPPGVTWCYY517 pKa = 11.14 GSLDD521 pKa = 4.28 CII523 pKa = 4.39
Molecular weight: 58.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.299
IPC2_protein 9.984
IPC_protein 11.038
Toseland 11.067
ProMoST 11.169
Dawson 11.125
Bjellqvist 10.965
Wikipedia 11.447
Rodwell 11.155
Grimsley 11.169
Solomon 11.403
Lehninger 11.33
Nozaki 11.052
DTASelect 10.965
Thurlkill 11.067
EMBOSS 11.506
Sillero 11.082
Patrickios 10.847
IPC_peptide 11.403
IPC2_peptide 10.233
IPC2.peptide.svr19 8.574
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
2611
92
566
373.0
41.84
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.4 ± 0.662
2.106 ± 0.6
6.09 ± 0.912
5.668 ± 0.349
4.098 ± 0.562
6.817 ± 0.917
2.375 ± 0.327
4.251 ± 0.76
4.787 ± 0.793
8.771 ± 0.727
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.379 ± 0.252
3.983 ± 0.77
7.085 ± 1.56
4.787 ± 0.658
7.124 ± 1.416
9.307 ± 1.489
5.553 ± 0.514
5.898 ± 0.57
1.34 ± 0.3
3.179 ± 0.414
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here