Amino acid dipepetide frequency for Microbacterium sp. No. 7

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
21.894AlaAla: 21.894 ± 0.205
0.796AlaCys: 0.796 ± 0.024
8.714AlaAsp: 8.714 ± 0.071
8.541AlaGlu: 8.541 ± 0.096
4.09AlaPhe: 4.09 ± 0.06
13.132AlaGly: 13.132 ± 0.119
2.81AlaHis: 2.81 ± 0.056
6.018AlaIle: 6.018 ± 0.074
2.232AlaLys: 2.232 ± 0.052
14.957AlaLeu: 14.957 ± 0.14
2.673AlaMet: 2.673 ± 0.05
2.1AlaAsn: 2.1 ± 0.049
7.102AlaPro: 7.102 ± 0.097
4.07AlaGln: 4.07 ± 0.058
10.376AlaArg: 10.376 ± 0.119
7.128AlaSer: 7.128 ± 0.078
7.488AlaThr: 7.488 ± 0.085
12.188AlaVal: 12.188 ± 0.124
2.155AlaTrp: 2.155 ± 0.043
2.432AlaTyr: 2.432 ± 0.041
0.0AlaXaa: 0.0 ± 0.0
Cys
0.81CysAla: 0.81 ± 0.027
0.056CysCys: 0.056 ± 0.008
0.364CysAsp: 0.364 ± 0.019
0.292CysGlu: 0.292 ± 0.017
0.172CysPhe: 0.172 ± 0.011
0.649CysGly: 0.649 ± 0.022
0.133CysHis: 0.133 ± 0.011
0.199CysIle: 0.199 ± 0.013
0.057CysLys: 0.057 ± 0.007
0.451CysLeu: 0.451 ± 0.018
0.096CysMet: 0.096 ± 0.01
0.097CysAsn: 0.097 ± 0.008
0.285CysPro: 0.285 ± 0.016
0.129CysGln: 0.129 ± 0.011
0.395CysArg: 0.395 ± 0.018
0.323CysSer: 0.323 ± 0.019
0.341CysThr: 0.341 ± 0.018
0.442CysVal: 0.442 ± 0.018
0.068CysTrp: 0.068 ± 0.007
0.116CysTyr: 0.116 ± 0.011
0.0CysXaa: 0.0 ± 0.0
Asp
9.901AspAla: 9.901 ± 0.106
0.245AspCys: 0.245 ± 0.013
4.607AspAsp: 4.607 ± 0.079
4.632AspGlu: 4.632 ± 0.065
1.553AspPhe: 1.553 ± 0.036
6.188AspGly: 6.188 ± 0.07
1.175AspHis: 1.175 ± 0.026
2.401AspIle: 2.401 ± 0.046
0.848AspLys: 0.848 ± 0.03
6.151AspLeu: 6.151 ± 0.069
0.84AspMet: 0.84 ± 0.026
0.877AspAsn: 0.877 ± 0.027
4.078AspPro: 4.078 ± 0.057
1.356AspGln: 1.356 ± 0.037
4.76AspArg: 4.76 ± 0.075
2.03AspSer: 2.03 ± 0.04
2.773AspThr: 2.773 ± 0.049
5.695AspVal: 5.695 ± 0.07
0.889AspTrp: 0.889 ± 0.027
1.191AspTyr: 1.191 ± 0.032
0.0AspXaa: 0.0 ± 0.0
Glu
7.116GluAla: 7.116 ± 0.07
0.277GluCys: 0.277 ± 0.014
2.488GluAsp: 2.488 ± 0.045
2.935GluGlu: 2.935 ± 0.059
1.555GluPhe: 1.555 ± 0.042
4.186GluGly: 4.186 ± 0.06
1.553GluHis: 1.553 ± 0.036
2.937GluIle: 2.937 ± 0.052
1.222GluLys: 1.222 ± 0.04
6.44GluLeu: 6.44 ± 0.076
0.849GluMet: 0.849 ± 0.026
1.172GluAsn: 1.172 ± 0.033
3.04GluPro: 3.04 ± 0.057
2.252GluGln: 2.252 ± 0.046
5.685GluArg: 5.685 ± 0.084
2.551GluSer: 2.551 ± 0.048
3.175GluThr: 3.175 ± 0.054
4.471GluVal: 4.471 ± 0.066
0.904GluTrp: 0.904 ± 0.026
1.099GluTyr: 1.099 ± 0.031
0.0GluXaa: 0.0 ± 0.0
Phe
4.478PheAla: 4.478 ± 0.064
0.161PheCys: 0.161 ± 0.011
2.331PheAsp: 2.331 ± 0.045
1.678PheGlu: 1.678 ± 0.039
1.016PhePhe: 1.016 ± 0.033
3.194PheGly: 3.194 ± 0.057
0.531PheHis: 0.531 ± 0.02
1.044PheIle: 1.044 ± 0.03
0.374PheLys: 0.374 ± 0.018
2.651PheLeu: 2.651 ± 0.05
0.415PheMet: 0.415 ± 0.017
0.562PheAsn: 0.562 ± 0.021
1.347PhePro: 1.347 ± 0.031
0.69PheGln: 0.69 ± 0.025
1.877PheArg: 1.877 ± 0.037
1.675PheSer: 1.675 ± 0.037
2.007PheThr: 2.007 ± 0.042
2.847PheVal: 2.847 ± 0.05
0.467PheTrp: 0.467 ± 0.02
0.609PheTyr: 0.609 ± 0.024
0.0PheXaa: 0.0 ± 0.0
Gly
11.439GlyAla: 11.439 ± 0.094
0.594GlyCys: 0.594 ± 0.02
5.381GlyAsp: 5.381 ± 0.07
5.092GlyGlu: 5.092 ± 0.065
3.224GlyPhe: 3.224 ± 0.05
8.364GlyGly: 8.364 ± 0.102
1.831GlyHis: 1.831 ± 0.039
4.829GlyIle: 4.829 ± 0.064
1.963GlyLys: 1.963 ± 0.045
8.846GlyLeu: 8.846 ± 0.096
2.027GlyMet: 2.027 ± 0.043
1.496GlyAsn: 1.496 ± 0.039
4.028GlyPro: 4.028 ± 0.063
2.419GlyGln: 2.419 ± 0.051
6.688GlyArg: 6.688 ± 0.078
4.988GlySer: 4.988 ± 0.07
5.575GlyThr: 5.575 ± 0.07
8.207GlyVal: 8.207 ± 0.088
1.621GlyTrp: 1.621 ± 0.033
2.178GlyTyr: 2.178 ± 0.044
0.0GlyXaa: 0.0 ± 0.0
His
2.787HisAla: 2.787 ± 0.047
0.128HisCys: 0.128 ± 0.011
1.468HisAsp: 1.468 ± 0.033
1.202HisGlu: 1.202 ± 0.029
0.518HisPhe: 0.518 ± 0.019
2.103HisGly: 2.103 ± 0.04
0.517HisHis: 0.517 ± 0.022
0.684HisIle: 0.684 ± 0.021
0.244HisLys: 0.244 ± 0.015
1.956HisLeu: 1.956 ± 0.039
0.302HisMet: 0.302 ± 0.017
0.32HisAsn: 0.32 ± 0.013
1.54HisPro: 1.54 ± 0.036
0.46HisGln: 0.46 ± 0.022
1.761HisArg: 1.761 ± 0.038
0.864HisSer: 0.864 ± 0.027
1.034HisThr: 1.034 ± 0.032
1.86HisVal: 1.86 ± 0.036
0.273HisTrp: 0.273 ± 0.016
0.436HisTyr: 0.436 ± 0.018
0.0HisXaa: 0.0 ± 0.0
Ile
7.368IleAla: 7.368 ± 0.094
0.233IleCys: 0.233 ± 0.014
3.44IleAsp: 3.44 ± 0.056
2.724IleGlu: 2.724 ± 0.052
0.977IlePhe: 0.977 ± 0.032
4.6IleGly: 4.6 ± 0.067
0.671IleHis: 0.671 ± 0.025
1.592IleIle: 1.592 ± 0.044
0.671IleLys: 0.671 ± 0.024
3.425IleLeu: 3.425 ± 0.053
0.643IleMet: 0.643 ± 0.022
0.861IleAsn: 0.861 ± 0.027
2.301IlePro: 2.301 ± 0.045
0.936IleGln: 0.936 ± 0.029
2.733IleArg: 2.733 ± 0.038
2.184IleSer: 2.184 ± 0.045
2.611IleThr: 2.611 ± 0.044
5.074IleVal: 5.074 ± 0.069
0.478IleTrp: 0.478 ± 0.02
0.685IleTyr: 0.685 ± 0.024
0.0IleXaa: 0.0 ± 0.0
Lys
2.151LysAla: 2.151 ± 0.05
0.064LysCys: 0.064 ± 0.007
0.914LysAsp: 0.914 ± 0.033
0.874LysGlu: 0.874 ± 0.031
0.362LysPhe: 0.362 ± 0.016
1.47LysGly: 1.47 ± 0.035
0.398LysHis: 0.398 ± 0.019
0.803LysIle: 0.803 ± 0.03
0.626LysLys: 0.626 ± 0.028
1.526LysLeu: 1.526 ± 0.043
0.298LysMet: 0.298 ± 0.015
0.411LysAsn: 0.411 ± 0.017
0.954LysPro: 0.954 ± 0.033
0.564LysGln: 0.564 ± 0.025
1.41LysArg: 1.41 ± 0.04
0.941LysSer: 0.941 ± 0.031
1.168LysThr: 1.168 ± 0.034
1.4LysVal: 1.4 ± 0.041
0.221LysTrp: 0.221 ± 0.013
0.385LysTyr: 0.385 ± 0.018
0.0LysXaa: 0.0 ± 0.0
Leu
15.077LeuAla: 15.077 ± 0.144
0.577LeuCys: 0.577 ± 0.021
6.724LeuAsp: 6.724 ± 0.074
4.942LeuGlu: 4.942 ± 0.068
2.826LeuPhe: 2.826 ± 0.049
9.135LeuGly: 9.135 ± 0.096
1.931LeuHis: 1.931 ± 0.04
4.188LeuIle: 4.188 ± 0.069
1.44LeuLys: 1.44 ± 0.038
10.273LeuLeu: 10.273 ± 0.119
1.58LeuMet: 1.58 ± 0.032
1.649LeuAsn: 1.649 ± 0.037
5.626LeuPro: 5.626 ± 0.07
2.45LeuGln: 2.45 ± 0.042
8.014LeuArg: 8.014 ± 0.086
5.744LeuSer: 5.744 ± 0.067
6.313LeuThr: 6.313 ± 0.078
9.67LeuVal: 9.67 ± 0.115
1.334LeuTrp: 1.334 ± 0.036
1.63LeuTyr: 1.63 ± 0.041
0.0LeuXaa: 0.0 ± 0.0
Met
1.991MetAla: 1.991 ± 0.045
0.107MetCys: 0.107 ± 0.012
0.801MetAsp: 0.801 ± 0.026
0.609MetGlu: 0.609 ± 0.021
0.512MetPhe: 0.512 ± 0.022
1.258MetGly: 1.258 ± 0.031
0.346MetHis: 0.346 ± 0.017
0.871MetIle: 0.871 ± 0.028
0.374MetLys: 0.374 ± 0.017
1.961MetLeu: 1.961 ± 0.041
0.349MetMet: 0.349 ± 0.019
0.462MetAsn: 0.462 ± 0.02
1.179MetPro: 1.179 ± 0.031
0.513MetGln: 0.513 ± 0.018
1.493MetArg: 1.493 ± 0.037
1.569MetSer: 1.569 ± 0.037
1.709MetThr: 1.709 ± 0.037
1.223MetVal: 1.223 ± 0.03
0.211MetTrp: 0.211 ± 0.011
0.284MetTyr: 0.284 ± 0.015
0.0MetXaa: 0.0 ± 0.0
Asn
2.45AsnAla: 2.45 ± 0.044
0.108AsnCys: 0.108 ± 0.009
1.035AsnAsp: 1.035 ± 0.025
0.897AsnGlu: 0.897 ± 0.027
0.507AsnPhe: 0.507 ± 0.02
1.707AsnGly: 1.707 ± 0.04
0.302AsnHis: 0.302 ± 0.016
0.745AsnIle: 0.745 ± 0.026
0.321AsnLys: 0.321 ± 0.019
1.753AsnLeu: 1.753 ± 0.046
0.298AsnMet: 0.298 ± 0.015
0.398AsnAsn: 0.398 ± 0.018
1.456AsnPro: 1.456 ± 0.036
0.447AsnGln: 0.447 ± 0.019
1.164AsnArg: 1.164 ± 0.029
0.791AsnSer: 0.791 ± 0.029
1.009AsnThr: 1.009 ± 0.031
1.614AsnVal: 1.614 ± 0.034
0.268AsnTrp: 0.268 ± 0.015
0.462AsnTyr: 0.462 ± 0.019
0.0AsnXaa: 0.0 ± 0.0
Pro
8.003ProAla: 8.003 ± 0.094
0.214ProCys: 0.214 ± 0.014
3.896ProAsp: 3.896 ± 0.055
3.678ProGlu: 3.678 ± 0.06
1.654ProPhe: 1.654 ± 0.031
5.36ProGly: 5.36 ± 0.073
1.264ProHis: 1.264 ± 0.037
1.994ProIle: 1.994 ± 0.037
0.866ProLys: 0.866 ± 0.029
5.066ProLeu: 5.066 ± 0.062
0.909ProMet: 0.909 ± 0.025
0.945ProAsn: 0.945 ± 0.027
2.777ProPro: 2.777 ± 0.067
1.52ProGln: 1.52 ± 0.039
3.906ProArg: 3.906 ± 0.058
2.986ProSer: 2.986 ± 0.047
3.171ProThr: 3.171 ± 0.09
5.067ProVal: 5.067 ± 0.068
0.835ProTrp: 0.835 ± 0.025
1.08ProTyr: 1.08 ± 0.029
0.0ProXaa: 0.0 ± 0.0
Gln
3.528GlnAla: 3.528 ± 0.058
0.136GlnCys: 0.136 ± 0.011
1.16GlnAsp: 1.16 ± 0.033
1.238GlnGlu: 1.238 ± 0.032
0.775GlnPhe: 0.775 ± 0.024
1.936GlnGly: 1.936 ± 0.037
0.679GlnHis: 0.679 ± 0.022
1.401GlnIle: 1.401 ± 0.041
0.561GlnLys: 0.561 ± 0.021
3.022GlnLeu: 3.022 ± 0.053
0.487GlnMet: 0.487 ± 0.02
0.568GlnAsn: 0.568 ± 0.024
1.479GlnPro: 1.479 ± 0.037
1.132GlnGln: 1.132 ± 0.033
2.72GlnArg: 2.72 ± 0.047
1.339GlnSer: 1.339 ± 0.033
1.501GlnThr: 1.501 ± 0.034
2.166GlnVal: 2.166 ± 0.038
0.488GlnTrp: 0.488 ± 0.019
0.589GlnTyr: 0.589 ± 0.024
0.0GlnXaa: 0.0 ± 0.0
Arg
10.22ArgAla: 10.22 ± 0.111
0.382ArgCys: 0.382 ± 0.018
4.631ArgAsp: 4.631 ± 0.061
4.67ArgGlu: 4.67 ± 0.07
2.553ArgPhe: 2.553 ± 0.041
5.95ArgGly: 5.95 ± 0.069
1.766ArgHis: 1.766 ± 0.034
3.754ArgIle: 3.754 ± 0.056
1.313ArgLys: 1.313 ± 0.037
8.087ArgLeu: 8.087 ± 0.086
1.804ArgMet: 1.804 ± 0.04
1.283ArgAsn: 1.283 ± 0.028
4.015ArgPro: 4.015 ± 0.063
1.972ArgGln: 1.972 ± 0.044
7.424ArgArg: 7.424 ± 0.102
3.884ArgSer: 3.884 ± 0.054
4.569ArgThr: 4.569 ± 0.055
6.578ArgVal: 6.578 ± 0.082
1.267ArgTrp: 1.267 ± 0.032
1.587ArgTyr: 1.587 ± 0.034
0.0ArgXaa: 0.0 ± 0.0
Ser
6.93SerAla: 6.93 ± 0.076
0.304SerCys: 0.304 ± 0.018
2.994SerAsp: 2.994 ± 0.05
2.386SerGlu: 2.386 ± 0.044
1.778SerPhe: 1.778 ± 0.038
5.516SerGly: 5.516 ± 0.078
0.957SerHis: 0.957 ± 0.031
2.435SerIle: 2.435 ± 0.047
0.909SerLys: 0.909 ± 0.029
5.249SerLeu: 5.249 ± 0.071
1.153SerMet: 1.153 ± 0.03
0.951SerAsn: 0.951 ± 0.03
3.072SerPro: 3.072 ± 0.055
1.259SerGln: 1.259 ± 0.033
3.681SerArg: 3.681 ± 0.064
3.159SerSer: 3.159 ± 0.056
3.21SerThr: 3.21 ± 0.05
4.367SerVal: 4.367 ± 0.063
0.815SerTrp: 0.815 ± 0.024
1.068SerTyr: 1.068 ± 0.03
0.0SerXaa: 0.0 ± 0.0
Thr
7.829ThrAla: 7.829 ± 0.092
0.304ThrCys: 0.304 ± 0.015
3.382ThrAsp: 3.382 ± 0.054
2.869ThrGlu: 2.869 ± 0.05
1.753ThrPhe: 1.753 ± 0.035
5.898ThrGly: 5.898 ± 0.068
1.18ThrHis: 1.18 ± 0.03
2.891ThrIle: 2.891 ± 0.047
0.947ThrLys: 0.947 ± 0.027
5.883ThrLeu: 5.883 ± 0.079
1.014ThrMet: 1.014 ± 0.031
1.045ThrAsn: 1.045 ± 0.033
4.144ThrPro: 4.144 ± 0.095
1.397ThrGln: 1.397 ± 0.035
4.061ThrArg: 4.061 ± 0.062
3.23ThrSer: 3.23 ± 0.05
3.781ThrThr: 3.781 ± 0.072
5.777ThrVal: 5.777 ± 0.076
0.883ThrTrp: 0.883 ± 0.027
1.105ThrTyr: 1.105 ± 0.028
0.0ThrXaa: 0.0 ± 0.0
Val
12.728ValAla: 12.728 ± 0.126
0.549ValCys: 0.549 ± 0.023
5.799ValAsp: 5.799 ± 0.068
4.826ValGlu: 4.826 ± 0.065
2.918ValPhe: 2.918 ± 0.051
7.162ValGly: 7.162 ± 0.081
1.733ValHis: 1.733 ± 0.041
4.006ValIle: 4.006 ± 0.056
1.399ValLys: 1.399 ± 0.041
9.596ValLeu: 9.596 ± 0.109
1.431ValMet: 1.431 ± 0.038
1.709ValAsn: 1.709 ± 0.041
5.004ValPro: 5.004 ± 0.074
2.241ValGln: 2.241 ± 0.04
6.61ValArg: 6.61 ± 0.073
4.941ValSer: 4.941 ± 0.063
5.878ValThr: 5.878 ± 0.083
9.361ValVal: 9.361 ± 0.106
1.277ValTrp: 1.277 ± 0.038
1.615ValTyr: 1.615 ± 0.036
0.0ValXaa: 0.0 ± 0.0
Trp
1.694TrpAla: 1.694 ± 0.038
0.114TrpCys: 0.114 ± 0.01
0.804TrpAsp: 0.804 ± 0.026
0.677TrpGlu: 0.677 ± 0.023
0.543TrpPhe: 0.543 ± 0.021
1.085TrpGly: 1.085 ± 0.029
0.367TrpHis: 0.367 ± 0.018
0.715TrpIle: 0.715 ± 0.027
0.286TrpLys: 0.286 ± 0.016
1.754TrpLeu: 1.754 ± 0.037
0.378TrpMet: 0.378 ± 0.019
0.425TrpAsn: 0.425 ± 0.016
0.758TrpPro: 0.758 ± 0.026
0.549TrpGln: 0.549 ± 0.02
1.301TrpArg: 1.301 ± 0.035
0.914TrpSer: 0.914 ± 0.029
0.946TrpThr: 0.946 ± 0.029
1.15TrpVal: 1.15 ± 0.033
0.34TrpTrp: 0.34 ± 0.017
0.286TrpTyr: 0.286 ± 0.015
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.501TyrAla: 2.501 ± 0.051
0.124TyrCys: 0.124 ± 0.01
1.257TyrAsp: 1.257 ± 0.031
1.144TyrGlu: 1.144 ± 0.035
0.67TyrPhe: 0.67 ± 0.023
1.898TyrGly: 1.898 ± 0.041
0.316TyrHis: 0.316 ± 0.016
0.701TyrIle: 0.701 ± 0.021
0.288TyrLys: 0.288 ± 0.018
2.077TyrLeu: 2.077 ± 0.038
0.276TyrMet: 0.276 ± 0.016
0.399TyrAsn: 0.399 ± 0.02
1.007TyrPro: 1.007 ± 0.029
0.549TyrGln: 0.549 ± 0.018
1.65TyrArg: 1.65 ± 0.038
0.951TyrSer: 0.951 ± 0.029
1.113TyrThr: 1.113 ± 0.033
1.625TyrVal: 1.625 ± 0.039
0.291TyrTrp: 0.291 ± 0.015
0.432TyrTyr: 0.432 ± 0.021
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4065 proteins (1310660 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski