Canine adenovirus serotype 2 (CAdV-2) (Canine adenovirus 2)
Average proteome isoelectric point is 6.6
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 17 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q76SA8|Q76SA8_ADEC2 Putative E3 region ORF2 OS=Canine adenovirus serotype 2 OX=10514 PE=4 SV=1
MM1 pKa = 7.47 AALGVSMGACFCLRR15 pKa = 11.84 LHH17 pKa = 6.58 KK18 pKa = 10.81 SLVEE22 pKa = 4.16 SVCAQLEE29 pKa = 4.39 LTDD32 pKa = 4.45 FLPSEE37 pKa = 4.18 LRR39 pKa = 11.84 RR40 pKa = 11.84 VVFSLIRR47 pKa = 11.84 AQEE50 pKa = 3.92 VRR52 pKa = 11.84 PCPTFAAAVSVLSGDD67 pKa = 3.54 YY68 pKa = 11.26 LEE70 pKa = 5.63 FYY72 pKa = 10.85 LFIGSDD78 pKa = 3.58 TEE80 pKa = 4.61 GFGSVADD87 pKa = 3.84 IQATAVSLQEE97 pKa = 3.52 ALRR100 pKa = 11.84 RR101 pKa = 11.84 DD102 pKa = 4.24 FCQLIPEE109 pKa = 4.75 DD110 pKa = 4.32 CAHH113 pKa = 7.02 LALGLSVTWEE123 pKa = 3.86 PNILVTAEE131 pKa = 3.71
Molecular weight: 14.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.376
IPC2_protein 4.584
IPC_protein 4.444
Toseland 4.291
ProMoST 4.469
Dawson 4.38
Bjellqvist 4.596
Wikipedia 4.253
Rodwell 4.291
Grimsley 4.215
Solomon 4.38
Lehninger 4.329
Nozaki 4.495
DTASelect 4.622
Thurlkill 4.304
EMBOSS 4.266
Sillero 4.558
Patrickios 2.994
IPC_peptide 4.393
IPC2_peptide 4.546
IPC2.peptide.svr19 4.502
Protein with the highest isoelectric point:
>tr|P87562|P87562_ADEC2 Hexon protein OS=Canine adenovirus serotype 2 OX=10514 GN=L3 PE=2 SV=1
MM1 pKa = 7.77 AGRR4 pKa = 11.84 NVTLRR9 pKa = 11.84 LRR11 pKa = 11.84 VPVRR15 pKa = 11.84 TKK17 pKa = 9.3 ITGAGRR23 pKa = 11.84 RR24 pKa = 11.84 RR25 pKa = 11.84 GRR27 pKa = 11.84 RR28 pKa = 11.84 PRR30 pKa = 11.84 GIRR33 pKa = 11.84 CGRR36 pKa = 11.84 MRR38 pKa = 11.84 GGFLPALIPLIAAAIGAVPGIASVALQAARR68 pKa = 11.84 HH69 pKa = 4.96
Molecular weight: 7.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.463
IPC2_protein 11.008
IPC_protein 12.618
Toseland 12.778
ProMoST 13.276
Dawson 12.778
Bjellqvist 12.778
Wikipedia 13.261
Rodwell 12.31
Grimsley 12.822
Solomon 13.276
Lehninger 13.173
Nozaki 12.778
DTASelect 12.778
Thurlkill 12.778
EMBOSS 13.276
Sillero 12.778
Patrickios 12.047
IPC_peptide 13.276
IPC2_peptide 12.266
IPC2.peptide.svr19 9.149
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
16
1
17
5895
55
905
346.8
38.91
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.871 ± 0.402
1.425 ± 0.344
4.36 ± 0.25
6.378 ± 0.725
4.224 ± 0.321
5.7 ± 0.338
2.358 ± 0.226
4.139 ± 0.29
4.224 ± 0.56
9.5 ± 0.566
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.884 ± 0.163
5.327 ± 0.633
6.429 ± 0.423
4.122 ± 0.328
6.497 ± 0.874
7.226 ± 0.365
5.818 ± 0.308
7.023 ± 0.358
1.187 ± 0.121
3.308 ± 0.493
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here