Avon-Heathcote Estuary associated circular virus 16

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; unclassified Circoviridae

Average proteome isoelectric point is 7.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0C5IBQ8|A0A0C5IBQ8_9CIRC ATP-dependent helicase Rep OS=Avon-Heathcote Estuary associated circular virus 16 OX=1618239 PE=3 SV=1
MM1 pKa = 8.0ADD3 pKa = 3.67RR4 pKa = 11.84RR5 pKa = 11.84PHH7 pKa = 6.61KK8 pKa = 10.5SWTYY12 pKa = 9.61TLNEE16 pKa = 4.14PTDD19 pKa = 3.59EE20 pKa = 4.48DD21 pKa = 3.92EE22 pKa = 5.02NRR24 pKa = 11.84IHH26 pKa = 6.7LWDD29 pKa = 3.71VSRR32 pKa = 11.84QIYY35 pKa = 8.89GHH37 pKa = 5.54EE38 pKa = 4.09TAPTTGQHH46 pKa = 5.97HH47 pKa = 6.25LQGFVSFRR55 pKa = 11.84AAKK58 pKa = 10.09RR59 pKa = 11.84FTGVTRR65 pKa = 11.84LLPRR69 pKa = 11.84AHH71 pKa = 6.78WEE73 pKa = 3.91VAKK76 pKa = 10.64YY77 pKa = 9.78PSSAWDD83 pKa = 3.55YY84 pKa = 8.3CTKK87 pKa = 10.87GDD89 pKa = 4.61DD90 pKa = 4.76LYY92 pKa = 11.87VVDD95 pKa = 4.21NRR97 pKa = 11.84VGRR100 pKa = 11.84GTRR103 pKa = 11.84SDD105 pKa = 3.32WQDD108 pKa = 3.13VLDD111 pKa = 4.61LVSEE115 pKa = 4.53GASDD119 pKa = 3.9AAILRR124 pKa = 11.84AQPSCFFKK132 pKa = 11.09YY133 pKa = 10.28GAGISRR139 pKa = 11.84ARR141 pKa = 11.84AALQEE146 pKa = 4.22PRR148 pKa = 11.84HH149 pKa = 5.78HH150 pKa = 5.89QTLCEE155 pKa = 4.13WYY157 pKa = 9.76YY158 pKa = 11.43GPTGGGKK165 pKa = 7.87STYY168 pKa = 9.42IAQEE172 pKa = 3.82HH173 pKa = 7.05PEE175 pKa = 4.26ADD177 pKa = 2.78WCQITKK183 pKa = 10.5SGFLLGYY190 pKa = 9.13HH191 pKa = 6.46NKK193 pKa = 8.0PTVVFDD199 pKa = 6.06DD200 pKa = 4.55PDD202 pKa = 4.6LDD204 pKa = 3.76AFGRR208 pKa = 11.84EE209 pKa = 3.91LFLNLVNRR217 pKa = 11.84TPFEE221 pKa = 4.29MNVKK225 pKa = 8.31GTSVAFNAKK234 pKa = 9.8LVIVASNKK242 pKa = 9.96HH243 pKa = 6.21PDD245 pKa = 3.38CFIPGDD251 pKa = 3.7AAVHH255 pKa = 5.31RR256 pKa = 11.84RR257 pKa = 11.84IHH259 pKa = 5.17KK260 pKa = 8.68TFLVSCIDD268 pKa = 3.6GEE270 pKa = 4.91HH271 pKa = 6.12MVEE274 pKa = 4.22QQQ276 pKa = 3.08

Molecular weight: 31.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C5IBQ8|A0A0C5IBQ8_9CIRC ATP-dependent helicase Rep OS=Avon-Heathcote Estuary associated circular virus 16 OX=1618239 PE=3 SV=1
MM1 pKa = 7.39ARR3 pKa = 11.84ATVRR7 pKa = 11.84QGNVALAKK15 pKa = 9.65RR16 pKa = 11.84RR17 pKa = 11.84RR18 pKa = 11.84RR19 pKa = 11.84ARR21 pKa = 11.84GRR23 pKa = 11.84KK24 pKa = 8.24IRR26 pKa = 11.84RR27 pKa = 11.84KK28 pKa = 9.44GRR30 pKa = 11.84RR31 pKa = 11.84KK32 pKa = 10.04AITARR37 pKa = 11.84RR38 pKa = 11.84VKK40 pKa = 10.68AIVSKK45 pKa = 10.67SISKK49 pKa = 10.28RR50 pKa = 11.84VEE52 pKa = 3.71TKK54 pKa = 9.14TQVIDD59 pKa = 3.27VTTASGGTWYY69 pKa = 10.65GFTINEE75 pKa = 4.13IPQGDD80 pKa = 3.83TSITRR85 pKa = 11.84DD86 pKa = 3.38GTSVFMKK93 pKa = 10.39SLQLNIMNVMSATTPTGTAYY113 pKa = 10.66DD114 pKa = 3.77YY115 pKa = 11.15MRR117 pKa = 11.84FILVNFPDD125 pKa = 4.87GEE127 pKa = 4.27QGDD130 pKa = 4.11ITDD133 pKa = 3.87VLAIDD138 pKa = 4.19TAPTSASALTMPYY151 pKa = 10.37RR152 pKa = 11.84MKK154 pKa = 10.68RR155 pKa = 11.84DD156 pKa = 3.56TVGVATHH163 pKa = 6.22TIKK166 pKa = 11.15YY167 pKa = 9.32QVLADD172 pKa = 3.25WHH174 pKa = 6.26EE175 pKa = 4.22LLNEE179 pKa = 4.06STHH182 pKa = 7.66AIDD185 pKa = 5.36LNRR188 pKa = 11.84LRR190 pKa = 11.84LKK192 pKa = 10.44INKK195 pKa = 9.03KK196 pKa = 8.22CTYY199 pKa = 11.05ALGNTDD205 pKa = 3.11GSVITKK211 pKa = 9.88NRR213 pKa = 11.84IRR215 pKa = 11.84LFYY218 pKa = 10.97YY219 pKa = 9.72GLQSIAEE226 pKa = 4.29TGTNIKK232 pKa = 9.99GRR234 pKa = 11.84LYY236 pKa = 10.26FTDD239 pKa = 3.25SS240 pKa = 3.11

Molecular weight: 26.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

516

240

276

258.0

29.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.333 ± 0.0

1.357 ± 0.629

6.395 ± 0.654

3.876 ± 0.92

3.682 ± 0.791

7.171 ± 0.058

3.295 ± 1.367

5.62 ± 1.536

5.426 ± 0.829

6.783 ± 0.078

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.938 ± 0.654

3.876 ± 0.473

3.682 ± 1.069

3.488 ± 0.382

8.333 ± 1.115

5.62 ± 0.143

9.109 ± 1.989

6.589 ± 0.227

1.744 ± 0.609

3.682 ± 0.045
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski