Chagres virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Ellioviricetes; Bunyavirales; Phenuiviridae; Phlebovirus; Chagres phlebovirus

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I1T327|I1T327_9VIRU Nonstructural protein OS=Chagres virus OX=629727 PE=4 SV=1
MM1 pKa = 7.65NYY3 pKa = 10.03YY4 pKa = 9.43ATDD7 pKa = 3.36IPLVSFSACQLRR19 pKa = 11.84RR20 pKa = 11.84IQVDD24 pKa = 3.46YY25 pKa = 11.35IPFNKK30 pKa = 9.62QATEE34 pKa = 3.64PHH36 pKa = 6.05SNYY39 pKa = 10.58SGMEE43 pKa = 4.06FPVTSYY49 pKa = 9.65TQVPGIRR56 pKa = 11.84SKK58 pKa = 11.28LQDD61 pKa = 4.15FYY63 pKa = 12.05NHH65 pKa = 5.96NQLPVSWGSGRR76 pKa = 11.84PGVVNDD82 pKa = 3.97SSSKK86 pKa = 9.8YY87 pKa = 9.42EE88 pKa = 3.92SLIKK92 pKa = 10.29EE93 pKa = 3.81ISKK96 pKa = 11.05LEE98 pKa = 3.78LRR100 pKa = 11.84DD101 pKa = 3.75VVKK104 pKa = 10.89HH105 pKa = 5.37NEE107 pKa = 3.8PNIRR111 pKa = 11.84RR112 pKa = 11.84ALCWPYY118 pKa = 11.04SYY120 pKa = 8.1PTLIFFDD127 pKa = 3.62LCGNKK132 pKa = 10.21DD133 pKa = 3.71SMGPWMFKK141 pKa = 9.82GAAMTSFMRR150 pKa = 11.84AGKK153 pKa = 9.48CAQIDD158 pKa = 3.55RR159 pKa = 11.84SLVNFHH165 pKa = 6.6RR166 pKa = 11.84MIVRR170 pKa = 11.84EE171 pKa = 4.3AIEE174 pKa = 4.2LGEE177 pKa = 4.27NEE179 pKa = 5.01AEE181 pKa = 4.2FTGKK185 pKa = 10.16DD186 pKa = 3.43IIMEE190 pKa = 4.5CCSVICRR197 pKa = 11.84RR198 pKa = 11.84LLRR201 pKa = 11.84AYY203 pKa = 9.69PFDD206 pKa = 3.49RR207 pKa = 11.84TYY209 pKa = 11.47DD210 pKa = 3.67KK211 pKa = 10.9PHH213 pKa = 6.34SRR215 pKa = 11.84LLTILEE221 pKa = 4.57DD222 pKa = 3.95FDD224 pKa = 4.07NHH226 pKa = 6.64FKK228 pKa = 10.78FDD230 pKa = 3.83YY231 pKa = 10.96GEE233 pKa = 4.14SSEE236 pKa = 4.78LRR238 pKa = 11.84VAVDD242 pKa = 3.39EE243 pKa = 4.8FDD245 pKa = 3.06IHH247 pKa = 5.08MKK249 pKa = 10.22RR250 pKa = 11.84RR251 pKa = 11.84EE252 pKa = 4.07AEE254 pKa = 3.92EE255 pKa = 4.36TFWGSDD261 pKa = 3.49FTDD264 pKa = 3.28SDD266 pKa = 3.72

Molecular weight: 30.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I1T327|I1T327_9VIRU Nonstructural protein OS=Chagres virus OX=629727 PE=4 SV=1
MM1 pKa = 7.86ANYY4 pKa = 10.15QDD6 pKa = 3.55IAVEE10 pKa = 4.11FAGDD14 pKa = 3.99LPTEE18 pKa = 4.62AIIRR22 pKa = 11.84TWVEE26 pKa = 3.31EE27 pKa = 3.76FAYY30 pKa = 10.44QGFDD34 pKa = 2.76ASQVLRR40 pKa = 11.84ILAEE44 pKa = 3.86RR45 pKa = 11.84GGANWRR51 pKa = 11.84EE52 pKa = 4.2DD53 pKa = 3.62AKK55 pKa = 11.53KK56 pKa = 10.09MIILSLTRR64 pKa = 11.84GNKK67 pKa = 7.45PSKK70 pKa = 9.53MMEE73 pKa = 4.03RR74 pKa = 11.84MSEE77 pKa = 3.8AGKK80 pKa = 9.13ATVSSLMTRR89 pKa = 11.84YY90 pKa = 9.42NLKK93 pKa = 10.32SGNPGRR99 pKa = 11.84NDD101 pKa = 3.09LTLSRR106 pKa = 11.84VATALAGWTCQAIPIVQDD124 pKa = 3.51FLPVTGVVMDD134 pKa = 4.81GLSPAYY140 pKa = 8.45PRR142 pKa = 11.84PMMHH146 pKa = 7.03PCFAGLIDD154 pKa = 4.25PSLPKK159 pKa = 9.68KK160 pKa = 9.13TVDD163 pKa = 3.35EE164 pKa = 4.24LVAAFSLYY172 pKa = 9.55MLQFSKK178 pKa = 9.92TINPSLRR185 pKa = 11.84AAKK188 pKa = 10.25RR189 pKa = 11.84EE190 pKa = 4.15DD191 pKa = 3.68IISSFRR197 pKa = 11.84QPMQAAINSTFLTGAQRR214 pKa = 11.84RR215 pKa = 11.84QFLRR219 pKa = 11.84TLGVVNDD226 pKa = 4.02NLEE229 pKa = 4.09PSQNVVAAARR239 pKa = 11.84VFRR242 pKa = 11.84GTGVV246 pKa = 2.84

Molecular weight: 27.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

3909

246

2093

977.3

110.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.347 ± 0.696

2.814 ± 0.663

5.475 ± 0.43

6.395 ± 0.527

4.528 ± 0.434

5.602 ± 0.186

2.584 ± 0.287

7.061 ± 0.377

6.344 ± 0.462

8.647 ± 0.274

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.223 ± 0.165

4.63 ± 0.04

3.837 ± 0.332

3.377 ± 0.23

5.091 ± 0.554

9.849 ± 0.787

5.04 ± 0.169

6.012 ± 0.295

1.228 ± 0.1

2.916 ± 0.256
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski