Elderberry carlavirus C
Average proteome isoelectric point is 7.01
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0A7MB61|A0A0A7MB61_9VIRU Helicase OS=Elderberry carlavirus C OX=1569054 PE=3 SV=1
MM1 pKa = 7.61 EE2 pKa = 5.1 IVLEE6 pKa = 4.18 EE7 pKa = 4.17 ADD9 pKa = 3.46 LAGFEE14 pKa = 4.19 RR15 pKa = 11.84 TNLKK19 pKa = 10.17 LSKK22 pKa = 9.93 PLVFHH27 pKa = 6.58 CVPGAGKK34 pKa = 8.5 STFIRR39 pKa = 11.84 HH40 pKa = 5.82 LLGLSSRR47 pKa = 11.84 FEE49 pKa = 4.32 AFTHH53 pKa = 6.64 GAADD57 pKa = 3.69 PRR59 pKa = 11.84 KK60 pKa = 9.85 LDD62 pKa = 3.32 GRR64 pKa = 11.84 RR65 pKa = 11.84 IKK67 pKa = 10.38 PVSEE71 pKa = 5.01 LEE73 pKa = 4.23 AADD76 pKa = 3.77 QNSLVLIDD84 pKa = 4.79 EE85 pKa = 4.55 YY86 pKa = 11.56 CSGEE90 pKa = 4.28 EE91 pKa = 3.87 VSEE94 pKa = 3.96 RR95 pKa = 11.84 ALAVFGDD102 pKa = 4.26 PLQSNTGPVLQPHH115 pKa = 5.98 FVLNKK120 pKa = 9.11 SRR122 pKa = 11.84 RR123 pKa = 11.84 FGAQTAALLKK133 pKa = 9.37 TLGYY137 pKa = 10.44 DD138 pKa = 3.59 VEE140 pKa = 5.73 AEE142 pKa = 3.86 GDD144 pKa = 4.1 DD145 pKa = 3.62 NVKK148 pKa = 10.32 ISDD151 pKa = 3.51 IFKK154 pKa = 10.59 AEE156 pKa = 3.97 PEE158 pKa = 4.34 GKK160 pKa = 9.58 VICYY164 pKa = 8.61 EE165 pKa = 4.13 RR166 pKa = 11.84 EE167 pKa = 3.89 VAQLLRR173 pKa = 11.84 RR174 pKa = 11.84 HH175 pKa = 5.61 GVDD178 pKa = 3.34 YY179 pKa = 11.39 LCANSVRR186 pKa = 11.84 GSTFDD191 pKa = 3.31 EE192 pKa = 4.96 VCFVTAEE199 pKa = 4.25 SEE201 pKa = 4.15 PSDD204 pKa = 3.3 RR205 pKa = 11.84 ALAFVCLTRR214 pKa = 11.84 HH215 pKa = 5.76 RR216 pKa = 11.84 KK217 pKa = 8.81 KK218 pKa = 10.98 LQILCPNATYY228 pKa = 10.9 APAA231 pKa = 5.32
Molecular weight: 25.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.525
IPC2_protein 5.55
IPC_protein 5.525
Toseland 5.753
ProMoST 5.804
Dawson 5.69
Bjellqvist 5.741
Wikipedia 5.652
Rodwell 5.652
Grimsley 5.817
Solomon 5.69
Lehninger 5.664
Nozaki 5.906
DTASelect 6.071
Thurlkill 6.008
EMBOSS 5.97
Sillero 6.008
Patrickios 4.202
IPC_peptide 5.703
IPC2_peptide 6.008
IPC2.peptide.svr19 6.019
Protein with the highest isoelectric point:
>tr|A0A0A7MDT6|A0A0A7MDT6_9VIRU Capsid protein OS=Elderberry carlavirus C OX=1569054 PE=3 SV=1
MM1 pKa = 7.54 GSDD4 pKa = 3.85 RR5 pKa = 11.84 LSTMYY10 pKa = 10.88 LVLWKK15 pKa = 10.08 LKK17 pKa = 10.36 NVGSIDD23 pKa = 3.96 LCVLILSLSKK33 pKa = 10.78 PPLTLGGGSSSYY45 pKa = 10.66 AKK47 pKa = 9.99 KK48 pKa = 10.27 RR49 pKa = 11.84 RR50 pKa = 11.84 AAKK53 pKa = 9.98 IGRR56 pKa = 11.84 CWRR59 pKa = 11.84 CFRR62 pKa = 11.84 VVPPPYY68 pKa = 9.71 HH69 pKa = 6.4 SKK71 pKa = 10.95 CDD73 pKa = 3.46 GRR75 pKa = 11.84 TCRR78 pKa = 11.84 PGISYY83 pKa = 9.84 RR84 pKa = 11.84 WEE86 pKa = 3.33 IALYY90 pKa = 9.35 ISRR93 pKa = 11.84 GVTEE97 pKa = 5.16 VIPLEE102 pKa = 4.18 NN103 pKa = 3.53
Molecular weight: 11.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.267
IPC2_protein 9.355
IPC_protein 9.487
Toseland 9.926
ProMoST 9.692
Dawson 10.175
Bjellqvist 9.926
Wikipedia 10.35
Rodwell 10.467
Grimsley 10.248
Solomon 10.218
Lehninger 10.175
Nozaki 10.072
DTASelect 9.882
Thurlkill 10.014
EMBOSS 10.35
Sillero 10.116
Patrickios 9.97
IPC_peptide 10.204
IPC2_peptide 9.077
IPC2.peptide.svr19 8.124
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
2818
66
2005
469.7
52.43
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.588 ± 0.714
2.697 ± 0.622
4.507 ± 0.556
7.062 ± 1.285
4.01 ± 0.83
6.352 ± 0.383
3.016 ± 0.305
5.287 ± 0.698
5.855 ± 0.805
10.256 ± 0.648
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.916 ± 0.369
3.655 ± 0.338
4.684 ± 0.822
2.484 ± 0.333
5.997 ± 0.502
6.813 ± 0.456
5.678 ± 0.61
6.565 ± 0.73
0.923 ± 0.258
3.655 ± 0.333
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here