Martelella mediterranea
Average proteome isoelectric point is 6.24
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4157 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4V6P0A7|A0A4V6P0A7_9RHIZ Threonine/homoserine efflux transporter RhtA OS=Martelella mediterranea OX=293089 GN=EDC90_10076 PE=4 SV=1
MM1 pKa = 7.7 RR2 pKa = 11.84 KK3 pKa = 9.32 VLLTSLGATSLAFLAGCGGGGGSNGVASNAVGYY36 pKa = 10.17 YY37 pKa = 10.53 ADD39 pKa = 3.65 TNFYY43 pKa = 10.85 SDD45 pKa = 4.21 RR46 pKa = 11.84 FDD48 pKa = 3.45 GSGRR52 pKa = 11.84 SKK54 pKa = 11.65 ANISLNDD61 pKa = 4.01 DD62 pKa = 3.62 DD63 pKa = 5.3 SVTVRR68 pKa = 11.84 VDD70 pKa = 3.14 EE71 pKa = 4.85 GPYY74 pKa = 10.58 SGDD77 pKa = 3.16 EE78 pKa = 3.65 ATFYY82 pKa = 11.27 YY83 pKa = 10.38 DD84 pKa = 3.49 YY85 pKa = 11.71 GSDD88 pKa = 3.52 QYY90 pKa = 11.85 VADD93 pKa = 4.84 LDD95 pKa = 4.42 DD96 pKa = 3.97 GARR99 pKa = 11.84 AYY101 pKa = 9.4 MVLDD105 pKa = 3.92 SFDD108 pKa = 4.4 DD109 pKa = 4.08 PSINGDD115 pKa = 3.72 EE116 pKa = 4.22 PVVALIEE123 pKa = 4.16 VDD125 pKa = 3.54 GGGTNSTFSTYY136 pKa = 10.35 RR137 pKa = 11.84 ATEE140 pKa = 3.82 GMTTNMPRR148 pKa = 11.84 AGTARR153 pKa = 11.84 YY154 pKa = 9.08 EE155 pKa = 4.31 GGHH158 pKa = 6.23 FGIAAIPGDD167 pKa = 3.98 DD168 pKa = 3.79 VYY170 pKa = 11.32 DD171 pKa = 3.76 IEE173 pKa = 5.5 GSFAANVDD181 pKa = 4.14 FGNASMNGAMATSVGDD197 pKa = 3.5 VTFDD201 pKa = 4.07 GSIDD205 pKa = 3.73 GPNFSSNNGSIALNGDD221 pKa = 3.38 RR222 pKa = 11.84 SLIDD226 pKa = 3.21 QGASGVDD233 pKa = 2.98 GGFYY237 pKa = 10.75 GDD239 pKa = 3.95 NAKK242 pKa = 10.4 GMAGTYY248 pKa = 10.01 LINGDD253 pKa = 4.1 NGLDD257 pKa = 3.64 GAGMLGNFVGEE268 pKa = 4.32 RR269 pKa = 11.84 TRR271 pKa = 4.05
Molecular weight: 27.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.711
IPC2_protein 3.732
IPC_protein 3.77
Toseland 3.528
ProMoST 3.948
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.745
Rodwell 3.579
Grimsley 3.427
Solomon 3.77
Lehninger 3.732
Nozaki 3.884
DTASelect 4.202
Thurlkill 3.592
EMBOSS 3.757
Sillero 3.897
Patrickios 1.901
IPC_peptide 3.757
IPC2_peptide 3.859
IPC2.peptide.svr19 3.787
Protein with the highest isoelectric point:
>tr|A0A4R3NUP0|A0A4R3NUP0_9RHIZ NAD(P)H dehydrogenase (Quinone) OS=Martelella mediterranea OX=293089 GN=EDC90_102063 PE=4 SV=1
MM1 pKa = 7.54 AKK3 pKa = 10.3 AADD6 pKa = 3.86 SRR8 pKa = 11.84 KK9 pKa = 6.72 TTAKK13 pKa = 9.91 RR14 pKa = 11.84 RR15 pKa = 11.84 AEE17 pKa = 3.86 RR18 pKa = 11.84 RR19 pKa = 11.84 GRR21 pKa = 11.84 RR22 pKa = 11.84 AEE24 pKa = 3.84 WLAAALLICKK34 pKa = 9.56 GYY36 pKa = 10.47 RR37 pKa = 11.84 IISMRR42 pKa = 11.84 YY43 pKa = 7.23 KK44 pKa = 10.51 CRR46 pKa = 11.84 AGEE49 pKa = 3.65 IDD51 pKa = 3.44 IIARR55 pKa = 11.84 KK56 pKa = 9.73 GDD58 pKa = 3.45 LAVFVEE64 pKa = 5.29 VKK66 pKa = 10.46 ARR68 pKa = 11.84 NHH70 pKa = 5.32 EE71 pKa = 4.05 QSAVDD76 pKa = 3.59 SVTPFSQHH84 pKa = 7.04 RR85 pKa = 11.84 IRR87 pKa = 11.84 AASDD91 pKa = 2.53 HH92 pKa = 5.6 WLARR96 pKa = 11.84 QRR98 pKa = 11.84 EE99 pKa = 4.4 GHH101 pKa = 5.61 TLSCRR106 pKa = 11.84 YY107 pKa = 10.0 DD108 pKa = 3.76 IIACLPRR115 pKa = 11.84 HH116 pKa = 6.21 LPRR119 pKa = 11.84 HH120 pKa = 5.56 FLDD123 pKa = 4.15 AFF125 pKa = 3.92
Molecular weight: 14.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.353
IPC2_protein 9.56
IPC_protein 10.379
Toseland 10.672
ProMoST 10.379
Dawson 10.76
Bjellqvist 10.496
Wikipedia 10.979
Rodwell 10.921
Grimsley 10.804
Solomon 10.906
Lehninger 10.862
Nozaki 10.687
DTASelect 10.482
Thurlkill 10.672
EMBOSS 11.082
Sillero 10.701
Patrickios 10.657
IPC_peptide 10.906
IPC2_peptide 9.706
IPC2.peptide.svr19 8.606
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4157
0
4157
1267225
27
2300
304.8
33.32
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.248 ± 0.046
0.835 ± 0.012
5.994 ± 0.035
6.208 ± 0.038
4.051 ± 0.029
8.193 ± 0.044
2.012 ± 0.018
5.69 ± 0.026
3.867 ± 0.029
9.699 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.726 ± 0.02
3.093 ± 0.021
4.761 ± 0.025
3.157 ± 0.022
6.411 ± 0.042
5.932 ± 0.029
5.328 ± 0.024
7.071 ± 0.034
1.281 ± 0.015
2.443 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here