Escherichia phage Lilleven

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; Bullavirinae; Alphatrevirus; unclassified Alphatrevirus

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6DYZ8|A0A4D6DYZ8_9VIRU External scaffolding protein D OS=Escherichia phage Lilleven OX=2562234 PE=3 SV=1
MM1 pKa = 7.33KK2 pKa = 9.29PAHH5 pKa = 6.63RR6 pKa = 11.84LYY8 pKa = 10.92HH9 pKa = 6.04PVLGSWLFKK18 pKa = 9.73CPVYY22 pKa = 10.42IRR24 pKa = 11.84YY25 pKa = 9.2EE26 pKa = 4.19SLHH29 pKa = 6.37HH30 pKa = 6.37GRR32 pKa = 11.84LIKK35 pKa = 10.53VRR37 pKa = 11.84AVRR40 pKa = 11.84DD41 pKa = 3.46LSPRR45 pKa = 11.84QQLIANPKK53 pKa = 7.92NCFDD57 pKa = 3.77VVIAEE62 pKa = 4.07IDD64 pKa = 3.74VTHH67 pKa = 7.24LSVTWYY73 pKa = 10.2DD74 pKa = 4.01FQAEE78 pKa = 4.59EE79 pKa = 4.53YY80 pKa = 10.12VEE82 pKa = 4.49CDD84 pKa = 2.88SGTMEE89 pKa = 5.48LLLSQVNSGEE99 pKa = 4.42VIRR102 pKa = 11.84SSEE105 pKa = 4.0DD106 pKa = 3.14LFVDD110 pKa = 3.34VDD112 pKa = 3.9DD113 pKa = 4.91MEE115 pKa = 4.22WLYY118 pKa = 11.07EE119 pKa = 4.0IVEE122 pKa = 4.23

Molecular weight: 14.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6DYR5|A0A4D6DYR5_9VIRU H protein OS=Escherichia phage Lilleven OX=2562234 PE=4 SV=1
MM1 pKa = 6.87EE2 pKa = 4.01TVIGKK7 pKa = 8.66FIALAVVALMLYY19 pKa = 10.27GISRR23 pKa = 11.84LIRR26 pKa = 11.84RR27 pKa = 11.84FPP29 pKa = 3.74

Molecular weight: 3.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

0

9

1871

29

494

207.9

23.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.071 ± 1.186

1.229 ± 0.33

6.36 ± 0.532

4.596 ± 0.61

4.009 ± 0.536

5.933 ± 1.048

2.512 ± 0.466

5.879 ± 0.651

5.024 ± 0.999

8.391 ± 0.822

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.153 ± 0.391

4.596 ± 0.432

4.115 ± 0.695

4.757 ± 0.771

6.093 ± 0.818

7.269 ± 0.82

6.04 ± 0.602

6.574 ± 1.103

1.55 ± 0.278

3.848 ± 0.59
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski