Capybara microvirus Cap3_SP_478
Average proteome isoelectric point is 5.76
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W630|A0A4P8W630_9VIRU Uncharacterized protein OS=Capybara microvirus Cap3_SP_478 OX=2585468 PE=4 SV=1
MM1 pKa = 7.62 VNIKK5 pKa = 10.47 YY6 pKa = 10.58 SDD8 pKa = 3.27 IAPILGEE15 pKa = 4.45 HH16 pKa = 5.37 YY17 pKa = 11.14 LEE19 pKa = 4.75 FKK21 pKa = 11.14 FNGVWLKK28 pKa = 10.11 TSILNNTDD36 pKa = 2.92 SNNIVSIFMLNGEE49 pKa = 4.55 DD50 pKa = 4.54 LYY52 pKa = 11.69 CEE54 pKa = 4.32 VKK56 pKa = 10.59 YY57 pKa = 10.74 HH58 pKa = 6.98 EE59 pKa = 4.61 LLQYY63 pKa = 11.21 VLVKK67 pKa = 9.58 HH68 pKa = 6.25 ICSDD72 pKa = 3.83 YY73 pKa = 11.47 YY74 pKa = 11.25 DD75 pKa = 4.36 IDD77 pKa = 3.05 WDD79 pKa = 3.65 YY80 pKa = 11.64 SYY82 pKa = 11.92 NEE84 pKa = 4.22 VIEE87 pKa = 4.82 CEE89 pKa = 4.32 CEE91 pKa = 4.19 VNFLNGEE98 pKa = 4.11 EE99 pKa = 4.18 NKK101 pKa = 10.29 FF102 pKa = 3.5
Molecular weight: 12.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.182
IPC2_protein 4.304
IPC_protein 4.202
Toseland 4.037
ProMoST 4.317
Dawson 4.151
Bjellqvist 4.304
Wikipedia 4.024
Rodwell 4.037
Grimsley 3.961
Solomon 4.139
Lehninger 4.101
Nozaki 4.266
DTASelect 4.406
Thurlkill 4.062
EMBOSS 4.05
Sillero 4.317
Patrickios 0.223
IPC_peptide 4.151
IPC2_peptide 4.304
IPC2.peptide.svr19 4.213
Protein with the highest isoelectric point:
>tr|A0A4P8W8R3|A0A4P8W8R3_9VIRU Major capsid protein OS=Capybara microvirus Cap3_SP_478 OX=2585468 PE=3 SV=1
MM1 pKa = 7.56 CVNPIKK7 pKa = 10.37 CWQYY11 pKa = 11.4 YY12 pKa = 6.65 EE13 pKa = 4.34 TDD15 pKa = 3.43 SLTGEE20 pKa = 4.42 EE21 pKa = 4.14 KK22 pKa = 9.28 THH24 pKa = 7.0 LVFNINKK31 pKa = 8.99 TSFNKK36 pKa = 10.63 DD37 pKa = 2.08 KK38 pKa = 10.85 DD39 pKa = 3.86 YY40 pKa = 11.54 SVEE43 pKa = 4.07 QKK45 pKa = 11.11 LEE47 pKa = 3.84 DD48 pKa = 3.84 LYY50 pKa = 10.97 IPGRR54 pKa = 11.84 NQKK57 pKa = 10.18 LYY59 pKa = 10.51 NRR61 pKa = 11.84 RR62 pKa = 11.84 IKK64 pKa = 10.5 PITLPCGKK72 pKa = 10.38 CPDD75 pKa = 4.08 CLMKK79 pKa = 10.94 YY80 pKa = 9.4 SLEE83 pKa = 3.64 WAYY86 pKa = 11.05 RR87 pKa = 11.84 IMNEE91 pKa = 3.3 ASYY94 pKa = 10.75 YY95 pKa = 10.99 AEE97 pKa = 4.13 NCFMTLTYY105 pKa = 11.19 ANTNGEE111 pKa = 4.37 LCLKK115 pKa = 10.36 DD116 pKa = 2.97 IQNFLKK122 pKa = 10.63 RR123 pKa = 11.84 LRR125 pKa = 11.84 KK126 pKa = 9.78 KK127 pKa = 10.5 ISPLKK132 pKa = 10.38 IRR134 pKa = 11.84 FFNCGEE140 pKa = 3.97 YY141 pKa = 10.46 GSKK144 pKa = 10.27 GKK146 pKa = 10.24 RR147 pKa = 11.84 PHH149 pKa = 5.59 FHH151 pKa = 7.07 CIIFGWCPSDD161 pKa = 4.85 LKK163 pKa = 10.97 FLKK166 pKa = 9.02 KK167 pKa = 8.76 TKK169 pKa = 9.72 KK170 pKa = 10.11 GQIIYY175 pKa = 9.24 TSEE178 pKa = 3.86 IVSSLWKK185 pKa = 10.35 HH186 pKa = 5.31 GFVSIGNVEE195 pKa = 4.19 FEE197 pKa = 4.41 SAKK200 pKa = 9.4 YY201 pKa = 7.37 TALYY205 pKa = 7.84 MQKK208 pKa = 10.01 LHH210 pKa = 6.84 KK211 pKa = 10.75 FPDD214 pKa = 3.55 KK215 pKa = 10.52 KK216 pKa = 10.76 VQPFLTMSNRR226 pKa = 11.84 PGIGYY231 pKa = 9.92 KK232 pKa = 10.22 YY233 pKa = 11.07 VLDD236 pKa = 4.17 KK237 pKa = 11.47 KK238 pKa = 10.16 NTLLLTDD245 pKa = 3.81 KK246 pKa = 10.84 VYY248 pKa = 11.53 NNGKK252 pKa = 9.94 DD253 pKa = 3.24 IPLPRR258 pKa = 11.84 YY259 pKa = 7.94 YY260 pKa = 10.98 LKK262 pKa = 10.89 VLEE265 pKa = 4.18 NQQYY269 pKa = 10.95 CDD271 pKa = 3.26 TSTLRR276 pKa = 11.84 VNRR279 pKa = 11.84 EE280 pKa = 3.65 KK281 pKa = 10.93 KK282 pKa = 9.81 KK283 pKa = 10.84 DD284 pKa = 3.91 LILEE288 pKa = 4.24 EE289 pKa = 4.4 EE290 pKa = 4.11 NVRR293 pKa = 11.84 AYY295 pKa = 9.88 KK296 pKa = 9.87 LAKK299 pKa = 9.52 YY300 pKa = 10.26 DD301 pKa = 5.32 KK302 pKa = 9.71 LLHH305 pKa = 6.06 WKK307 pKa = 10.05 LEE309 pKa = 4.26 KK310 pKa = 10.94 GDD312 pKa = 3.73 TT313 pKa = 3.72
Molecular weight: 37.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.767
IPC2_protein 8.741
IPC_protein 8.58
Toseland 9.253
ProMoST 9.107
Dawson 9.575
Bjellqvist 9.37
Wikipedia 9.736
Rodwell 9.838
Grimsley 9.633
Solomon 9.604
Lehninger 9.56
Nozaki 9.502
DTASelect 9.297
Thurlkill 9.428
EMBOSS 9.721
Sillero 9.575
Patrickios 4.482
IPC_peptide 9.589
IPC2_peptide 8.068
IPC2.peptide.svr19 7.797
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8
0
8
1609
77
543
201.1
22.91
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.588 ± 1.581
1.678 ± 0.581
6.091 ± 0.659
6.091 ± 1.249
4.288 ± 0.474
5.594 ± 0.762
1.492 ± 0.427
5.842 ± 0.649
7.396 ± 1.825
8.452 ± 0.932
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.175 ± 0.294
8.266 ± 0.802
3.045 ± 0.951
3.418 ± 0.613
3.418 ± 0.642
7.023 ± 1.02
5.158 ± 0.494
6.339 ± 0.874
1.305 ± 0.163
6.339 ± 0.354
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here