Streptomyces sp. SID4931
Average proteome isoelectric point is 6.27
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6148 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6G2UFT9|A0A6G2UFT9_9ACTN SAM-dependent methyltransferase (Fragment) OS=Streptomyces sp. SID4931 OX=2690277 GN=GTW37_04825 PE=4 SV=1
MM1 pKa = 7.73 SNPQQLRR8 pKa = 11.84 YY9 pKa = 10.06 SKK11 pKa = 9.49 EE12 pKa = 4.2 HH13 pKa = 5.77 EE14 pKa = 4.11 WLSAVEE20 pKa = 5.07 DD21 pKa = 4.42 GVATIGITEE30 pKa = 4.13 YY31 pKa = 11.03 AANALGDD38 pKa = 3.65 VVYY41 pKa = 10.63 AQLPEE46 pKa = 4.21 VGDD49 pKa = 3.7 TVTAGEE55 pKa = 4.44 TCGEE59 pKa = 4.13 LEE61 pKa = 4.31 STKK64 pKa = 10.61 SVSDD68 pKa = 4.05 LYY70 pKa = 11.58 SPVTGEE76 pKa = 3.73 VTAANQDD83 pKa = 3.78 VVDD86 pKa = 5.36 DD87 pKa = 4.32 PSLVNSAPFEE97 pKa = 4.42 GGWLFKK103 pKa = 11.05 VRR105 pKa = 11.84 VAEE108 pKa = 4.37 EE109 pKa = 3.93 PADD112 pKa = 3.89 LLSADD117 pKa = 4.59 EE118 pKa = 4.16 YY119 pKa = 11.13 TAFSGNN125 pKa = 3.27
Molecular weight: 13.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.767
IPC2_protein 3.808
IPC_protein 3.745
Toseland 3.554
ProMoST 3.884
Dawson 3.719
Bjellqvist 3.872
Wikipedia 3.617
Rodwell 3.579
Grimsley 3.478
Solomon 3.694
Lehninger 3.656
Nozaki 3.834
DTASelect 3.986
Thurlkill 3.605
EMBOSS 3.63
Sillero 3.859
Patrickios 1.837
IPC_peptide 3.694
IPC2_peptide 3.834
IPC2.peptide.svr19 3.771
Protein with the highest isoelectric point:
>tr|A0A6G2UST0|A0A6G2UST0_9ACTN Phospholipase (Fragment) OS=Streptomyces sp. SID4931 OX=2690277 GN=GTW37_34265 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.0 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 RR12 pKa = 11.84 RR13 pKa = 11.84 AKK15 pKa = 8.7 THH17 pKa = 5.15 GFRR20 pKa = 11.84 LRR22 pKa = 11.84 MRR24 pKa = 11.84 TRR26 pKa = 11.84 AGRR29 pKa = 11.84 AILANRR35 pKa = 11.84 RR36 pKa = 11.84 GKK38 pKa = 10.57 GRR40 pKa = 11.84 SSLSAA45 pKa = 3.62
Molecular weight: 5.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.272
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.647
Grimsley 13.086
Solomon 13.539
Lehninger 13.437
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.369
IPC_peptide 13.539
IPC2_peptide 12.53
IPC2.peptide.svr19 9.218
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6148
0
6148
1545259
21
3717
251.3
26.93
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.384 ± 0.048
0.78 ± 0.009
6.023 ± 0.027
5.92 ± 0.031
2.801 ± 0.018
9.43 ± 0.028
2.212 ± 0.015
3.396 ± 0.022
2.363 ± 0.031
10.375 ± 0.039
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.798 ± 0.013
1.832 ± 0.017
5.895 ± 0.028
2.721 ± 0.019
7.84 ± 0.038
5.163 ± 0.025
6.102 ± 0.027
8.421 ± 0.032
1.461 ± 0.014
2.083 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here