Feline calicivirus (strain Cat/United States/Urbana/1960) (FCV)
Average proteome isoelectric point is 6.92
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|Q66916|VP2_FCVUR Protein VP2 OS=Feline calicivirus (strain Cat/United States/Urbana/1960) OX=292349 GN=ORF3 PE=1 SV=1
MM1 pKa = 7.93 CSTCANVLKK10 pKa = 10.53 YY11 pKa = 10.45 YY12 pKa = 10.68 NWDD15 pKa = 3.24 PHH17 pKa = 6.57 FKK19 pKa = 10.75 LVINPNKK26 pKa = 9.64 FLSIGFCDD34 pKa = 4.01 NPLMCCYY41 pKa = 10.28 PEE43 pKa = 4.52 LLPEE47 pKa = 5.49 FGTVWDD53 pKa = 4.87 CDD55 pKa = 3.54 QSPLQIYY62 pKa = 10.1 LEE64 pKa = 4.78 SILGDD69 pKa = 4.18 DD70 pKa = 4.34 EE71 pKa = 4.37 WSSTYY76 pKa = 10.23 EE77 pKa = 4.27 AIDD80 pKa = 3.54 PVVPPMHH87 pKa = 6.63 WNEE90 pKa = 3.79 AGKK93 pKa = 10.0 IFQPHH98 pKa = 6.94 PGVLMHH104 pKa = 6.82 HH105 pKa = 7.38 IIGEE109 pKa = 4.26 VAKK112 pKa = 10.58 AWDD115 pKa = 3.79 PNLPLFRR122 pKa = 11.84 LEE124 pKa = 4.98 ADD126 pKa = 4.41 DD127 pKa = 5.31 GSITAPEE134 pKa = 3.98 QGTVVGGVIAEE145 pKa = 4.57 PSSQMSTAADD155 pKa = 3.57 MASGKK160 pKa = 10.41 SVDD163 pKa = 4.22 SEE165 pKa = 4.08 WEE167 pKa = 3.9 AFFSFHH173 pKa = 6.68 TSVNWSTSEE182 pKa = 4.04 TQGKK186 pKa = 9.14 ILFKK190 pKa = 10.81 QSLGPLLNPYY200 pKa = 10.02 LEE202 pKa = 4.97 HH203 pKa = 7.51 LSKK206 pKa = 11.04 LYY208 pKa = 10.13 VAWSGSVEE216 pKa = 3.89 VRR218 pKa = 11.84 FSISGSGVFGGKK230 pKa = 9.18 LAAIVVPPGVDD241 pKa = 4.14 PIQSTSMLQYY251 pKa = 9.97 PHH253 pKa = 7.23 VLFDD257 pKa = 3.64 ARR259 pKa = 11.84 QVEE262 pKa = 4.59 PVIFTIPDD270 pKa = 3.33 LRR272 pKa = 11.84 STLYY276 pKa = 10.94 HH277 pKa = 6.94 LMSDD281 pKa = 3.39 TDD283 pKa = 3.85 TTSLVIMVYY292 pKa = 10.82 NDD294 pKa = 5.27 LINPYY299 pKa = 10.63 ANDD302 pKa = 4.11 SNSSGCIVTVEE313 pKa = 4.42 TKK315 pKa = 10.21 PGSDD319 pKa = 3.78 FKK321 pKa = 11.38 FHH323 pKa = 6.82 LLKK326 pKa = 10.8 PPGSMLTHH334 pKa = 6.5 GSVPSDD340 pKa = 4.83 LIPKK344 pKa = 9.05 TSSLWIGNRR353 pKa = 11.84 FWSDD357 pKa = 2.1 ITDD360 pKa = 3.77 FVIRR364 pKa = 11.84 PFVFQANRR372 pKa = 11.84 HH373 pKa = 5.0 FDD375 pKa = 3.97 FNQEE379 pKa = 3.77 TAGWSTPRR387 pKa = 11.84 FRR389 pKa = 11.84 PITVTISEE397 pKa = 4.38 KK398 pKa = 10.83 NGAKK402 pKa = 10.27 LGVGVATDD410 pKa = 4.37 FIVPGIPDD418 pKa = 4.36 GWPDD422 pKa = 3.32 TTIGEE427 pKa = 4.51 KK428 pKa = 10.3 LVPAGDD434 pKa = 3.8 YY435 pKa = 11.01 AITNGSGNDD444 pKa = 2.89 ITTANQYY451 pKa = 10.38 DD452 pKa = 3.63 AADD455 pKa = 4.27 IIRR458 pKa = 11.84 NNTNFKK464 pKa = 10.68 GMYY467 pKa = 9.16 ICGSLQRR474 pKa = 11.84 AWGDD478 pKa = 3.38 KK479 pKa = 10.31 KK480 pKa = 10.73 ISNTAFITTATVEE493 pKa = 4.32 GNDD496 pKa = 4.81 LIPSNVIDD504 pKa = 3.57 QTKK507 pKa = 10.17 IAIFQDD513 pKa = 2.99 NHH515 pKa = 5.82 VQDD518 pKa = 4.55 EE519 pKa = 4.71 VQTSDD524 pKa = 4.02 DD525 pKa = 3.57 TLALLGYY532 pKa = 8.74 TGIGEE537 pKa = 4.21 EE538 pKa = 4.09 AIGANRR544 pKa = 11.84 EE545 pKa = 3.91 RR546 pKa = 11.84 VVRR549 pKa = 11.84 ISTLPEE555 pKa = 3.42 TGARR559 pKa = 11.84 GGNHH563 pKa = 7.11 PIFYY567 pKa = 10.22 KK568 pKa = 10.91 NSIKK572 pKa = 10.4 LGYY575 pKa = 9.15 VIRR578 pKa = 11.84 SIDD581 pKa = 3.5 VFNSQILHH589 pKa = 6.01 TSRR592 pKa = 11.84 QLSLNHH598 pKa = 6.17 YY599 pKa = 8.3 LLPPDD604 pKa = 3.46 SFAVYY609 pKa = 10.34 RR610 pKa = 11.84 IIDD613 pKa = 3.9 SNGSWFDD620 pKa = 3.28 VGIDD624 pKa = 3.52 FDD626 pKa = 4.01 GFSFVGVSDD635 pKa = 3.54 VGKK638 pKa = 11.02 LEE640 pKa = 4.2 FPLTASYY647 pKa = 9.91 MGIQLAKK654 pKa = 9.99 IRR656 pKa = 11.84 LASNIRR662 pKa = 11.84 STMTKK667 pKa = 10.33 LL668 pKa = 3.23
Molecular weight: 73.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.8
IPC2_protein 4.838
IPC_protein 4.825
Toseland 4.724
ProMoST 4.978
Dawson 4.825
Bjellqvist 4.952
Wikipedia 4.724
Rodwell 4.711
Grimsley 4.647
Solomon 4.825
Lehninger 4.787
Nozaki 4.94
DTASelect 5.156
Thurlkill 4.736
EMBOSS 4.762
Sillero 5.003
Patrickios 3.745
IPC_peptide 4.825
IPC2_peptide 4.991
IPC2.peptide.svr19 4.934
Protein with the highest isoelectric point:
>sp|Q66916|VP2_FCVUR Protein VP2 OS=Feline calicivirus (strain Cat/United States/Urbana/1960) OX=292349 GN=ORF3 PE=1 SV=1
MM1 pKa = 7.42 NSILGLIDD9 pKa = 3.47 TVTNTIGKK17 pKa = 8.41 AQQIEE22 pKa = 4.4 LDD24 pKa = 3.79 KK25 pKa = 11.3 AALGQQRR32 pKa = 11.84 EE33 pKa = 4.2 LALQRR38 pKa = 11.84 MNLDD42 pKa = 3.13 RR43 pKa = 11.84 QALNNQVEE51 pKa = 4.53 QFNKK55 pKa = 9.93 ILEE58 pKa = 4.14 QRR60 pKa = 11.84 VQGPLQSVRR69 pKa = 11.84 LARR72 pKa = 11.84 AAGFRR77 pKa = 11.84 VDD79 pKa = 3.85 PYY81 pKa = 11.45 SYY83 pKa = 10.68 TNQNFYY89 pKa = 11.26 DD90 pKa = 4.02 DD91 pKa = 3.8 QLNAIRR97 pKa = 11.84 LSYY100 pKa = 11.1 RR101 pKa = 11.84 NLFKK105 pKa = 11.17 NN106 pKa = 3.62
Molecular weight: 12.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.897
IPC2_protein 9.004
IPC_protein 9.194
Toseland 9.487
ProMoST 9.341
Dawson 9.794
Bjellqvist 9.516
Wikipedia 10.014
Rodwell 9.926
Grimsley 9.897
Solomon 9.897
Lehninger 9.853
Nozaki 9.428
DTASelect 9.516
Thurlkill 9.589
EMBOSS 9.911
Sillero 9.692
Patrickios 5.194
IPC_peptide 9.882
IPC2_peptide 8.17
IPC2.peptide.svr19 7.88
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
2537
106
1763
845.7
93.54
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.095 ± 0.687
2.05 ± 0.642
5.952 ± 0.382
4.612 ± 0.358
3.981 ± 0.62
6.74 ± 0.567
2.799 ± 0.612
6.425 ± 0.732
5.676 ± 1.038
8.593 ± 0.87
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.128 ± 0.111
4.375 ± 1.273
5.361 ± 0.707
3.114 ± 1.739
4.533 ± 0.935
8.356 ± 0.849
6.661 ± 0.54
6.937 ± 0.412
1.498 ± 0.359
3.114 ± 0.125
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here