Pseudomonas phage phiKZ

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Phikzvirus

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 369 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|L7SZ01|L7SZ01_BPDPK PHIKZ041.2 OS=Pseudomonas phage phiKZ OX=169683 PE=4 SV=1
MM1 pKa = 8.12DD2 pKa = 6.47LNPHH6 pKa = 7.16DD7 pKa = 5.43IDD9 pKa = 3.54NQAIFNYY16 pKa = 10.72LEE18 pKa = 4.09MLPSTFKK25 pKa = 9.6ITEE28 pKa = 4.27STFVAADD35 pKa = 3.7FDD37 pKa = 5.57SNGDD41 pKa = 3.59VIIQLEE47 pKa = 3.89QSLIYY52 pKa = 9.5TGCSLSKK59 pKa = 10.69FNACFNTMM67 pKa = 3.61

Molecular weight: 7.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q8SCU5|Q8SCU5_BPDPK PHIKZ217 OS=Pseudomonas phage phiKZ OX=169683 PE=4 SV=2
MM1 pKa = 7.64FKK3 pKa = 10.66RR4 pKa = 11.84VMRR7 pKa = 11.84YY8 pKa = 9.04IRR10 pKa = 11.84VLKK13 pKa = 10.34LIVIFYY19 pKa = 9.96VRR21 pKa = 11.84RR22 pKa = 11.84QLGLHH27 pKa = 7.03DD28 pKa = 3.67YY29 pKa = 7.54CTRR32 pKa = 11.84KK33 pKa = 8.38NTIALDD39 pKa = 3.46RR40 pKa = 11.84EE41 pKa = 4.15NNKK44 pKa = 9.62RR45 pKa = 11.84VQINAISRR53 pKa = 11.84KK54 pKa = 9.01VEE56 pKa = 3.57EE57 pKa = 4.34ALFKK61 pKa = 11.16VEE63 pKa = 4.96LVAIMSKK70 pKa = 10.32RR71 pKa = 3.28

Molecular weight: 8.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

369

0

369

85117

20

2237

230.7

26.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.139 ± 0.155

0.992 ± 0.048

6.681 ± 0.083

6.358 ± 0.151

4.014 ± 0.071

5.635 ± 0.136

2.034 ± 0.075

7.379 ± 0.104

6.482 ± 0.132

8.459 ± 0.099

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.709 ± 0.076

6.146 ± 0.107

4.019 ± 0.107

3.406 ± 0.089

4.717 ± 0.111

6.075 ± 0.107

6.254 ± 0.111

6.872 ± 0.113

1.299 ± 0.059

4.331 ± 0.117
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski