Rousettus aegyptiacus papillomavirus 1
Average proteome isoelectric point is 6.13
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q0QII1|Q0QII1_9PAPI Protein E6 OS=Rousettus aegyptiacus papillomavirus 1 OX=369584 GN=E6 PE=3 SV=1
MM1 pKa = 8.02 RR2 pKa = 11.84 GRR4 pKa = 11.84 DD5 pKa = 3.61 PSLEE9 pKa = 3.76 LAAAAEE15 pKa = 4.34 EE16 pKa = 4.39 VQAVDD21 pKa = 3.54 LHH23 pKa = 7.8 CDD25 pKa = 3.17 EE26 pKa = 5.91 SLDD29 pKa = 4.66 QEE31 pKa = 5.29 AEE33 pKa = 3.79 QQTFYY38 pKa = 11.23 NIQLPCTSCEE48 pKa = 3.3 RR49 pKa = 11.84 HH50 pKa = 4.9 MRR52 pKa = 11.84 LVISCSSSGLRR63 pKa = 11.84 TLAVLLRR70 pKa = 11.84 QRR72 pKa = 11.84 EE73 pKa = 4.15 VQLICAQCTRR83 pKa = 11.84 EE84 pKa = 3.84 FHH86 pKa = 6.54
Molecular weight: 9.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.88
IPC2_protein 5.067
IPC_protein 4.914
Toseland 4.851
ProMoST 4.94
Dawson 4.889
Bjellqvist 5.029
Wikipedia 4.749
Rodwell 4.825
Grimsley 4.774
Solomon 4.889
Lehninger 4.851
Nozaki 5.016
DTASelect 5.118
Thurlkill 4.863
EMBOSS 4.787
Sillero 5.092
Patrickios 3.427
IPC_peptide 4.902
IPC2_peptide 5.092
IPC2.peptide.svr19 5.06
Protein with the highest isoelectric point:
>tr|Q0QIH9|Q0QIH9_9PAPI Replication protein E1 OS=Rousettus aegyptiacus papillomavirus 1 OX=369584 GN=E1 PE=3 SV=1
MM1 pKa = 6.94 EE2 pKa = 4.74 TLRR5 pKa = 11.84 DD6 pKa = 3.87 RR7 pKa = 11.84 LDD9 pKa = 3.39 VLQDD13 pKa = 3.47 QILGHH18 pKa = 5.81 YY19 pKa = 7.6 EE20 pKa = 3.9 RR21 pKa = 11.84 QSTCLQDD28 pKa = 4.14 HH29 pKa = 4.87 VQYY32 pKa = 9.77 WGLVRR37 pKa = 11.84 RR38 pKa = 11.84 EE39 pKa = 3.97 SSLLFYY45 pKa = 11.15 ARR47 pKa = 11.84 GKK49 pKa = 9.63 GVKK52 pKa = 8.53 TLGYY56 pKa = 10.33 LPVPVQQVSQDD67 pKa = 3.28 RR68 pKa = 11.84 ARR70 pKa = 11.84 QAIQLHH76 pKa = 6.69 LATQSLAKK84 pKa = 9.93 SKK86 pKa = 11.22 YY87 pKa = 9.53 KK88 pKa = 10.54 DD89 pKa = 3.96 EE90 pKa = 4.52 PWTLQDD96 pKa = 3.46 VSRR99 pKa = 11.84 EE100 pKa = 4.02 NYY102 pKa = 8.98 MSEE105 pKa = 4.04 PKK107 pKa = 9.13 HH108 pKa = 4.91 TFKK111 pKa = 11.03 KK112 pKa = 10.49 GGTQVEE118 pKa = 4.62 VIYY121 pKa = 11.09 DD122 pKa = 3.64 GDD124 pKa = 3.83 EE125 pKa = 4.55 DD126 pKa = 4.79 NVMLYY131 pKa = 9.54 TSWEE135 pKa = 4.09 HH136 pKa = 8.36 IYY138 pKa = 10.51 IQDD141 pKa = 5.6 DD142 pKa = 3.55 DD143 pKa = 4.97 GSWYY147 pKa = 9.47 KK148 pKa = 10.62 RR149 pKa = 11.84 KK150 pKa = 10.73 GEE152 pKa = 4.04 VCVEE156 pKa = 3.66 GLYY159 pKa = 8.21 YY160 pKa = 10.22 TWLGQKK166 pKa = 7.79 TFYY169 pKa = 11.51 VNFEE173 pKa = 4.28 EE174 pKa = 4.9 EE175 pKa = 4.02 LGKK178 pKa = 10.96 YY179 pKa = 7.11 STRR182 pKa = 11.84 NVYY185 pKa = 8.39 VVRR188 pKa = 11.84 TADD191 pKa = 3.55 GEE193 pKa = 4.39 LMSFNPVTSSTPQEE207 pKa = 4.38 EE208 pKa = 4.85 GQQPGGGAPGHH219 pKa = 6.76 PGPPPRR225 pKa = 11.84 KK226 pKa = 8.45 RR227 pKa = 11.84 RR228 pKa = 11.84 RR229 pKa = 11.84 LFGEE233 pKa = 4.51 KK234 pKa = 9.38 PAEE237 pKa = 4.39 GPGGRR242 pKa = 11.84 RR243 pKa = 11.84 VPVPRR248 pKa = 11.84 PGGPPPGRR256 pKa = 11.84 RR257 pKa = 11.84 GGEE260 pKa = 4.0 TPSRR264 pKa = 11.84 GTEE267 pKa = 3.82 RR268 pKa = 11.84 PAEE271 pKa = 4.18 GSGGCIPPALPGAAGKK287 pKa = 8.19 TEE289 pKa = 3.85 EE290 pKa = 4.37 RR291 pKa = 11.84 GGAGFRR297 pKa = 11.84 PQPPTAVVAPVIIIAGPPNALKK319 pKa = 10.08 CQRR322 pKa = 11.84 YY323 pKa = 8.82 RR324 pKa = 11.84 LQNQHH329 pKa = 5.57 YY330 pKa = 8.98 RR331 pKa = 11.84 HH332 pKa = 6.33 FAQVTTTYY340 pKa = 9.54 HH341 pKa = 6.15 WLDD344 pKa = 3.43 AGHH347 pKa = 5.15 TQRR350 pKa = 11.84 RR351 pKa = 11.84 SAAQIVLRR359 pKa = 11.84 FVDD362 pKa = 3.86 TAQRR366 pKa = 11.84 EE367 pKa = 4.55 TFLNTGALSDD377 pKa = 3.63 RR378 pKa = 11.84 VHH380 pKa = 6.14 YY381 pKa = 10.8 VLGTMPFLSS390 pKa = 3.86
Molecular weight: 43.68 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.474
IPC2_protein 8.463
IPC_protein 8.448
Toseland 8.741
ProMoST 8.785
Dawson 9.151
Bjellqvist 9.077
Wikipedia 9.341
Rodwell 9.209
Grimsley 9.165
Solomon 9.238
Lehninger 9.18
Nozaki 9.063
DTASelect 8.99
Thurlkill 9.019
EMBOSS 9.253
Sillero 9.209
Patrickios 4.431
IPC_peptide 9.224
IPC2_peptide 7.907
IPC2.peptide.svr19 7.748
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
2507
86
665
358.1
39.67
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.781 ± 0.499
2.074 ± 0.472
6.143 ± 0.432
6.502 ± 0.44
3.151 ± 0.332
8.456 ± 0.571
2.513 ± 0.26
4.428 ± 0.662
3.949 ± 0.54
8.217 ± 0.551
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.034 ± 0.363
3.032 ± 0.632
7.06 ± 0.94
4.826 ± 0.658
6.781 ± 0.641
6.661 ± 0.734
5.943 ± 0.585
7.06 ± 0.468
1.197 ± 0.205
3.191 ± 0.356
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here