Gemmata sp. SH-PL17

Taxonomy: cellular organisms; Bacteria; PVC group; Planctomycetes; Planctomycetia; Gemmatales; Gemmataceae; Gemmata; unclassified Gemmata

Average proteome isoelectric point is 6.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7589 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A142XBY3|A0A142XBY3_9BACT Uncharacterized protein OS=Gemmata sp. SH-PL17 OX=1630693 GN=VT84_08540 PE=4 SV=1
MM1 pKa = 7.61ARR3 pKa = 11.84TRR5 pKa = 11.84PGAPDD10 pKa = 3.9EE11 pKa = 4.89DD12 pKa = 5.7DD13 pKa = 3.94EE14 pKa = 6.54DD15 pKa = 4.72EE16 pKa = 5.47FDD18 pKa = 4.65GDD20 pKa = 4.97DD21 pKa = 3.72YY22 pKa = 11.9DD23 pKa = 6.27AEE25 pKa = 4.29TDD27 pKa = 3.76YY28 pKa = 11.61DD29 pKa = 3.94PEE31 pKa = 5.42DD32 pKa = 4.51PEE34 pKa = 4.51TYY36 pKa = 9.89PAGLYY41 pKa = 10.39VDD43 pKa = 4.74DD44 pKa = 4.56SRR46 pKa = 11.84AFVPCPYY53 pKa = 9.96CRR55 pKa = 11.84AEE57 pKa = 3.8IDD59 pKa = 4.24EE60 pKa = 4.62EE61 pKa = 5.14AEE63 pKa = 4.07QCPKK67 pKa = 10.82CGMFVSRR74 pKa = 11.84EE75 pKa = 3.94DD76 pKa = 3.53APSDD80 pKa = 3.26RR81 pKa = 11.84KK82 pKa = 10.34SPVWIVLMVLALLAVMAMALGG103 pKa = 3.71

Molecular weight: 11.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A142XP97|A0A142XP97_9BACT Uncharacterized protein OS=Gemmata sp. SH-PL17 OX=1630693 GN=VT84_30665 PE=4 SV=1
MM1 pKa = 7.96PKK3 pKa = 9.87TIVAVRR9 pKa = 11.84RR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84PFIGEE17 pKa = 3.8PRR19 pKa = 11.84RR20 pKa = 11.84AAAVVSARR28 pKa = 11.84PPARR32 pKa = 11.84PLVVAQIRR40 pKa = 11.84RR41 pKa = 11.84THH43 pKa = 5.96VGRR46 pKa = 11.84GTIGRR51 pKa = 11.84PTKK54 pKa = 10.5LATPVVAKK62 pKa = 10.32RR63 pKa = 11.84PPAVLVAALRR73 pKa = 11.84RR74 pKa = 11.84PVRR77 pKa = 11.84PPFRR81 pKa = 11.84GFVGRR86 pKa = 11.84PTKK89 pKa = 10.81LSVAPAATTIPRR101 pKa = 11.84VLVVSQRR108 pKa = 11.84AQVLIIRR115 pKa = 11.84RR116 pKa = 11.84AFIGRR121 pKa = 11.84PARR124 pKa = 11.84AATTKK129 pKa = 10.27RR130 pKa = 11.84PPSVQVAALRR140 pKa = 11.84LARR143 pKa = 11.84FAGAARR149 pKa = 11.84LIRR152 pKa = 11.84TPVANAQPAPATGHH166 pKa = 5.94RR167 pKa = 11.84VTVIGRR173 pKa = 11.84LKK175 pKa = 10.83NRR177 pKa = 11.84LNVTGRR183 pKa = 11.84LWNVVTVIGRR193 pKa = 11.84GQPP196 pKa = 3.12

Molecular weight: 21.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7589

0

7589

2568544

29

3677

338.5

36.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.608 ± 0.032

1.074 ± 0.012

5.699 ± 0.019

5.664 ± 0.025

3.687 ± 0.017

8.357 ± 0.033

2.036 ± 0.012

3.824 ± 0.015

4.385 ± 0.033

9.707 ± 0.029

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.76 ± 0.01

2.822 ± 0.017

6.215 ± 0.029

2.969 ± 0.017

7.118 ± 0.028

5.068 ± 0.024

6.148 ± 0.037

8.051 ± 0.024

1.535 ± 0.012

2.273 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski