Lake Sarah-associated circular molecule 9

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 7.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A126GA21|A0A126GA21_9VIRU ATP-dependent helicase Rep OS=Lake Sarah-associated circular molecule 9 OX=1685734 PE=3 SV=1
MM1 pKa = 7.62SKK3 pKa = 10.85GNRR6 pKa = 11.84AWCYY10 pKa = 8.56TLNNYY15 pKa = 7.52TEE17 pKa = 4.32EE18 pKa = 4.27EE19 pKa = 4.09RR20 pKa = 11.84DD21 pKa = 3.48SLRR24 pKa = 11.84SLKK27 pKa = 10.3CAYY30 pKa = 9.82QVFGYY35 pKa = 10.34EE36 pKa = 3.98RR37 pKa = 11.84GAADD41 pKa = 3.75TPHH44 pKa = 6.32LQGYY48 pKa = 7.38VQFAHH53 pKa = 6.15QKK55 pKa = 8.0TLSAVKK61 pKa = 10.22KK62 pKa = 9.18LLPRR66 pKa = 11.84AHH68 pKa = 6.81LEE70 pKa = 3.97EE71 pKa = 4.21RR72 pKa = 11.84RR73 pKa = 11.84GTIDD77 pKa = 4.26QAVEE81 pKa = 3.96YY82 pKa = 9.63CKK84 pKa = 10.52KK85 pKa = 10.79DD86 pKa = 3.19GDD88 pKa = 3.81FEE90 pKa = 4.99EE91 pKa = 4.82YY92 pKa = 10.34GKK94 pKa = 11.05KK95 pKa = 10.16PMSQKK100 pKa = 10.71EE101 pKa = 3.93KK102 pKa = 10.88GKK104 pKa = 9.76EE105 pKa = 3.71EE106 pKa = 3.87KK107 pKa = 10.55NRR109 pKa = 11.84WKK111 pKa = 10.7RR112 pKa = 11.84ILEE115 pKa = 4.21KK116 pKa = 10.47ADD118 pKa = 3.58EE119 pKa = 4.81GDD121 pKa = 3.93EE122 pKa = 3.88EE123 pKa = 4.33WLRR126 pKa = 11.84EE127 pKa = 4.02NEE129 pKa = 3.8PNVAFKK135 pKa = 10.97HH136 pKa = 4.83MATFRR141 pKa = 11.84SHH143 pKa = 6.76KK144 pKa = 9.34KK145 pKa = 9.42PRR147 pKa = 11.84VGTLQYY153 pKa = 11.2EE154 pKa = 4.54EE155 pKa = 5.3TPHH158 pKa = 5.8EE159 pKa = 4.37WWVGPTGTGKK169 pKa = 9.97SRR171 pKa = 11.84KK172 pKa = 8.83AHH174 pKa = 5.54EE175 pKa = 4.93EE176 pKa = 4.26YY177 pKa = 9.97PNHH180 pKa = 6.13YY181 pKa = 10.49AKK183 pKa = 10.6EE184 pKa = 4.11KK185 pKa = 9.03NKK187 pKa = 8.6WWCGYY192 pKa = 8.35TGQEE196 pKa = 3.87TVIIEE201 pKa = 4.12EE202 pKa = 4.5ADD204 pKa = 3.7PKK206 pKa = 10.71TMEE209 pKa = 4.43HH210 pKa = 6.23LAARR214 pKa = 11.84LKK216 pKa = 10.41VWADD220 pKa = 3.92RR221 pKa = 11.84YY222 pKa = 9.85PFPGEE227 pKa = 3.63IKK229 pKa = 10.02GGRR232 pKa = 11.84IEE234 pKa = 4.69GIRR237 pKa = 11.84PLRR240 pKa = 11.84VIVISNYY247 pKa = 9.56TIEE250 pKa = 4.12EE251 pKa = 4.25CFANQNDD258 pKa = 3.94VEE260 pKa = 4.16PLRR263 pKa = 11.84RR264 pKa = 11.84RR265 pKa = 11.84FKK267 pKa = 9.93EE268 pKa = 3.96VKK270 pKa = 9.55FGEE273 pKa = 4.33RR274 pKa = 11.84TMPSPWHH281 pKa = 6.22PSYY284 pKa = 10.29TLL286 pKa = 3.27

Molecular weight:
33.64 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A126GA21|A0A126GA21_9VIRU ATP-dependent helicase Rep OS=Lake Sarah-associated circular molecule 9 OX=1685734 PE=3 SV=1
MM1 pKa = 7.62SKK3 pKa = 10.85GNRR6 pKa = 11.84AWCYY10 pKa = 8.56TLNNYY15 pKa = 7.52TEE17 pKa = 4.32EE18 pKa = 4.27EE19 pKa = 4.09RR20 pKa = 11.84DD21 pKa = 3.48SLRR24 pKa = 11.84SLKK27 pKa = 10.3CAYY30 pKa = 9.82QVFGYY35 pKa = 10.34EE36 pKa = 3.98RR37 pKa = 11.84GAADD41 pKa = 3.75TPHH44 pKa = 6.32LQGYY48 pKa = 7.38VQFAHH53 pKa = 6.15QKK55 pKa = 8.0TLSAVKK61 pKa = 10.22KK62 pKa = 9.18LLPRR66 pKa = 11.84AHH68 pKa = 6.81LEE70 pKa = 3.97EE71 pKa = 4.21RR72 pKa = 11.84RR73 pKa = 11.84GTIDD77 pKa = 4.26QAVEE81 pKa = 3.96YY82 pKa = 9.63CKK84 pKa = 10.52KK85 pKa = 10.79DD86 pKa = 3.19GDD88 pKa = 3.81FEE90 pKa = 4.99EE91 pKa = 4.82YY92 pKa = 10.34GKK94 pKa = 11.05KK95 pKa = 10.16PMSQKK100 pKa = 10.71EE101 pKa = 3.93KK102 pKa = 10.88GKK104 pKa = 9.76EE105 pKa = 3.71EE106 pKa = 3.87KK107 pKa = 10.55NRR109 pKa = 11.84WKK111 pKa = 10.7RR112 pKa = 11.84ILEE115 pKa = 4.21KK116 pKa = 10.47ADD118 pKa = 3.58EE119 pKa = 4.81GDD121 pKa = 3.93EE122 pKa = 3.88EE123 pKa = 4.33WLRR126 pKa = 11.84EE127 pKa = 4.02NEE129 pKa = 3.8PNVAFKK135 pKa = 10.97HH136 pKa = 4.83MATFRR141 pKa = 11.84SHH143 pKa = 6.76KK144 pKa = 9.34KK145 pKa = 9.42PRR147 pKa = 11.84VGTLQYY153 pKa = 11.2EE154 pKa = 4.54EE155 pKa = 5.3TPHH158 pKa = 5.8EE159 pKa = 4.37WWVGPTGTGKK169 pKa = 9.97SRR171 pKa = 11.84KK172 pKa = 8.83AHH174 pKa = 5.54EE175 pKa = 4.93EE176 pKa = 4.26YY177 pKa = 9.97PNHH180 pKa = 6.13YY181 pKa = 10.49AKK183 pKa = 10.6EE184 pKa = 4.11KK185 pKa = 9.03NKK187 pKa = 8.6WWCGYY192 pKa = 8.35TGQEE196 pKa = 3.87TVIIEE201 pKa = 4.12EE202 pKa = 4.5ADD204 pKa = 3.7PKK206 pKa = 10.71TMEE209 pKa = 4.43HH210 pKa = 6.23LAARR214 pKa = 11.84LKK216 pKa = 10.41VWADD220 pKa = 3.92RR221 pKa = 11.84YY222 pKa = 9.85PFPGEE227 pKa = 3.63IKK229 pKa = 10.02GGRR232 pKa = 11.84IEE234 pKa = 4.69GIRR237 pKa = 11.84PLRR240 pKa = 11.84VIVISNYY247 pKa = 9.56TIEE250 pKa = 4.12EE251 pKa = 4.25CFANQNDD258 pKa = 3.94VEE260 pKa = 4.16PLRR263 pKa = 11.84RR264 pKa = 11.84RR265 pKa = 11.84FKK267 pKa = 9.93EE268 pKa = 3.96VKK270 pKa = 9.55FGEE273 pKa = 4.33RR274 pKa = 11.84TMPSPWHH281 pKa = 6.22PSYY284 pKa = 10.29TLL286 pKa = 3.27

Molecular weight:
33.64 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1

0

1

286

286

286

286.0

33.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.294 ± 0.0

1.748 ± 0.0

3.497 ± 0.0

12.238 ± 0.0

3.147 ± 0.0

6.993 ± 0.0

3.497 ± 0.0

3.497 ± 0.0

9.79 ± 0.0

5.594 ± 0.0

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.748 ± 0.0

3.846 ± 0.0

5.594 ± 0.0

3.147 ± 0.0

7.692 ± 0.0

3.497 ± 0.0

5.594 ± 0.0

4.545 ± 0.0

3.147 ± 0.0

4.895 ± 0.0

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski