Lake Sarah-associated circular molecule 9
Average proteome isoelectric point is 7.68
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A126GA21|A0A126GA21_9VIRU ATP-dependent helicase Rep OS=Lake Sarah-associated circular molecule 9 OX=1685734 PE=3 SV=1
MM1 pKa = 7.62 SKK3 pKa = 10.85 GNRR6 pKa = 11.84 AWCYY10 pKa = 8.56 TLNNYY15 pKa = 7.52 TEE17 pKa = 4.32 EE18 pKa = 4.27 EE19 pKa = 4.09 RR20 pKa = 11.84 DD21 pKa = 3.48 SLRR24 pKa = 11.84 SLKK27 pKa = 10.3 CAYY30 pKa = 9.82 QVFGYY35 pKa = 10.34 EE36 pKa = 3.98 RR37 pKa = 11.84 GAADD41 pKa = 3.75 TPHH44 pKa = 6.32 LQGYY48 pKa = 7.38 VQFAHH53 pKa = 6.15 QKK55 pKa = 8.0 TLSAVKK61 pKa = 10.22 KK62 pKa = 9.18 LLPRR66 pKa = 11.84 AHH68 pKa = 6.81 LEE70 pKa = 3.97 EE71 pKa = 4.21 RR72 pKa = 11.84 RR73 pKa = 11.84 GTIDD77 pKa = 4.26 QAVEE81 pKa = 3.96 YY82 pKa = 9.63 CKK84 pKa = 10.52 KK85 pKa = 10.79 DD86 pKa = 3.19 GDD88 pKa = 3.81 FEE90 pKa = 4.99 EE91 pKa = 4.82 YY92 pKa = 10.34 GKK94 pKa = 11.05 KK95 pKa = 10.16 PMSQKK100 pKa = 10.71 EE101 pKa = 3.93 KK102 pKa = 10.88 GKK104 pKa = 9.76 EE105 pKa = 3.71 EE106 pKa = 3.87 KK107 pKa = 10.55 NRR109 pKa = 11.84 WKK111 pKa = 10.7 RR112 pKa = 11.84 ILEE115 pKa = 4.21 KK116 pKa = 10.47 ADD118 pKa = 3.58 EE119 pKa = 4.81 GDD121 pKa = 3.93 EE122 pKa = 3.88 EE123 pKa = 4.33 WLRR126 pKa = 11.84 EE127 pKa = 4.02 NEE129 pKa = 3.8 PNVAFKK135 pKa = 10.97 HH136 pKa = 4.83 MATFRR141 pKa = 11.84 SHH143 pKa = 6.76 KK144 pKa = 9.34 KK145 pKa = 9.42 PRR147 pKa = 11.84 VGTLQYY153 pKa = 11.2 EE154 pKa = 4.54 EE155 pKa = 5.3 TPHH158 pKa = 5.8 EE159 pKa = 4.37 WWVGPTGTGKK169 pKa = 9.97 SRR171 pKa = 11.84 KK172 pKa = 8.83 AHH174 pKa = 5.54 EE175 pKa = 4.93 EE176 pKa = 4.26 YY177 pKa = 9.97 PNHH180 pKa = 6.13 YY181 pKa = 10.49 AKK183 pKa = 10.6 EE184 pKa = 4.11 KK185 pKa = 9.03 NKK187 pKa = 8.6 WWCGYY192 pKa = 8.35 TGQEE196 pKa = 3.87 TVIIEE201 pKa = 4.12 EE202 pKa = 4.5 ADD204 pKa = 3.7 PKK206 pKa = 10.71 TMEE209 pKa = 4.43 HH210 pKa = 6.23 LAARR214 pKa = 11.84 LKK216 pKa = 10.41 VWADD220 pKa = 3.92 RR221 pKa = 11.84 YY222 pKa = 9.85 PFPGEE227 pKa = 3.63 IKK229 pKa = 10.02 GGRR232 pKa = 11.84 IEE234 pKa = 4.69 GIRR237 pKa = 11.84 PLRR240 pKa = 11.84 VIVISNYY247 pKa = 9.56 TIEE250 pKa = 4.12 EE251 pKa = 4.25 CFANQNDD258 pKa = 3.94 VEE260 pKa = 4.16 PLRR263 pKa = 11.84 RR264 pKa = 11.84 RR265 pKa = 11.84 FKK267 pKa = 9.93 EE268 pKa = 3.96 VKK270 pKa = 9.55 FGEE273 pKa = 4.33 RR274 pKa = 11.84 TMPSPWHH281 pKa = 6.22 PSYY284 pKa = 10.29 TLL286 pKa = 3.27
Molecular weight: 33.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.685
IPC2_protein 7.936
IPC_protein 7.849
Toseland 7.98
ProMoST 8.361
Dawson 8.639
Bjellqvist 8.756
Wikipedia 8.639
Rodwell 8.712
Grimsley 7.936
Solomon 8.77
Lehninger 8.756
Nozaki 8.887
DTASelect 8.58
Thurlkill 8.668
EMBOSS 8.829
Sillero 8.916
Patrickios 4.558
IPC_peptide 8.77
IPC2_peptide 7.512
IPC2.peptide.svr19 7.678
Protein with the highest isoelectric point:
>tr|A0A126GA21|A0A126GA21_9VIRU ATP-dependent helicase Rep OS=Lake Sarah-associated circular molecule 9 OX=1685734 PE=3 SV=1
MM1 pKa = 7.62 SKK3 pKa = 10.85 GNRR6 pKa = 11.84 AWCYY10 pKa = 8.56 TLNNYY15 pKa = 7.52 TEE17 pKa = 4.32 EE18 pKa = 4.27 EE19 pKa = 4.09 RR20 pKa = 11.84 DD21 pKa = 3.48 SLRR24 pKa = 11.84 SLKK27 pKa = 10.3 CAYY30 pKa = 9.82 QVFGYY35 pKa = 10.34 EE36 pKa = 3.98 RR37 pKa = 11.84 GAADD41 pKa = 3.75 TPHH44 pKa = 6.32 LQGYY48 pKa = 7.38 VQFAHH53 pKa = 6.15 QKK55 pKa = 8.0 TLSAVKK61 pKa = 10.22 KK62 pKa = 9.18 LLPRR66 pKa = 11.84 AHH68 pKa = 6.81 LEE70 pKa = 3.97 EE71 pKa = 4.21 RR72 pKa = 11.84 RR73 pKa = 11.84 GTIDD77 pKa = 4.26 QAVEE81 pKa = 3.96 YY82 pKa = 9.63 CKK84 pKa = 10.52 KK85 pKa = 10.79 DD86 pKa = 3.19 GDD88 pKa = 3.81 FEE90 pKa = 4.99 EE91 pKa = 4.82 YY92 pKa = 10.34 GKK94 pKa = 11.05 KK95 pKa = 10.16 PMSQKK100 pKa = 10.71 EE101 pKa = 3.93 KK102 pKa = 10.88 GKK104 pKa = 9.76 EE105 pKa = 3.71 EE106 pKa = 3.87 KK107 pKa = 10.55 NRR109 pKa = 11.84 WKK111 pKa = 10.7 RR112 pKa = 11.84 ILEE115 pKa = 4.21 KK116 pKa = 10.47 ADD118 pKa = 3.58 EE119 pKa = 4.81 GDD121 pKa = 3.93 EE122 pKa = 3.88 EE123 pKa = 4.33 WLRR126 pKa = 11.84 EE127 pKa = 4.02 NEE129 pKa = 3.8 PNVAFKK135 pKa = 10.97 HH136 pKa = 4.83 MATFRR141 pKa = 11.84 SHH143 pKa = 6.76 KK144 pKa = 9.34 KK145 pKa = 9.42 PRR147 pKa = 11.84 VGTLQYY153 pKa = 11.2 EE154 pKa = 4.54 EE155 pKa = 5.3 TPHH158 pKa = 5.8 EE159 pKa = 4.37 WWVGPTGTGKK169 pKa = 9.97 SRR171 pKa = 11.84 KK172 pKa = 8.83 AHH174 pKa = 5.54 EE175 pKa = 4.93 EE176 pKa = 4.26 YY177 pKa = 9.97 PNHH180 pKa = 6.13 YY181 pKa = 10.49 AKK183 pKa = 10.6 EE184 pKa = 4.11 KK185 pKa = 9.03 NKK187 pKa = 8.6 WWCGYY192 pKa = 8.35 TGQEE196 pKa = 3.87 TVIIEE201 pKa = 4.12 EE202 pKa = 4.5 ADD204 pKa = 3.7 PKK206 pKa = 10.71 TMEE209 pKa = 4.43 HH210 pKa = 6.23 LAARR214 pKa = 11.84 LKK216 pKa = 10.41 VWADD220 pKa = 3.92 RR221 pKa = 11.84 YY222 pKa = 9.85 PFPGEE227 pKa = 3.63 IKK229 pKa = 10.02 GGRR232 pKa = 11.84 IEE234 pKa = 4.69 GIRR237 pKa = 11.84 PLRR240 pKa = 11.84 VIVISNYY247 pKa = 9.56 TIEE250 pKa = 4.12 EE251 pKa = 4.25 CFANQNDD258 pKa = 3.94 VEE260 pKa = 4.16 PLRR263 pKa = 11.84 RR264 pKa = 11.84 RR265 pKa = 11.84 FKK267 pKa = 9.93 EE268 pKa = 3.96 VKK270 pKa = 9.55 FGEE273 pKa = 4.33 RR274 pKa = 11.84 TMPSPWHH281 pKa = 6.22 PSYY284 pKa = 10.29 TLL286 pKa = 3.27
Molecular weight: 33.64 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.685
IPC2_protein 7.936
IPC_protein 7.849
Toseland 7.98
ProMoST 8.361
Dawson 8.639
Bjellqvist 8.756
Wikipedia 8.639
Rodwell 8.712
Grimsley 7.936
Solomon 8.77
Lehninger 8.756
Nozaki 8.887
DTASelect 8.58
Thurlkill 8.668
EMBOSS 8.829
Sillero 8.916
Patrickios 4.558
IPC_peptide 8.77
IPC2_peptide 7.512
IPC2.peptide.svr19 7.678
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
286
286
286
286.0
33.64
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.294 ± 0.0
1.748 ± 0.0
3.497 ± 0.0
12.238 ± 0.0
3.147 ± 0.0
6.993 ± 0.0
3.497 ± 0.0
3.497 ± 0.0
9.79 ± 0.0
5.594 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.748 ± 0.0
3.846 ± 0.0
5.594 ± 0.0
3.147 ± 0.0
7.692 ± 0.0
3.497 ± 0.0
5.594 ± 0.0
4.545 ± 0.0
3.147 ± 0.0
4.895 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here