Sweet potato chlorotic stunt virus
Average proteome isoelectric point is 6.86
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q8JJW3|Q8JJW3_9CLOS Putative movement protein OS=Sweet potato chlorotic stunt virus OX=81931 GN=p60 PE=4 SV=1
MM1 pKa = 7.94 DD2 pKa = 4.55 FSEE5 pKa = 5.77 LIEE8 pKa = 4.41 KK9 pKa = 10.67 YY10 pKa = 9.3 GVEE13 pKa = 4.5 RR14 pKa = 11.84 ISSLTSRR21 pKa = 11.84 LLEE24 pKa = 3.93 IKK26 pKa = 9.46 RR27 pKa = 11.84 TGVGLVNLLLNLINEE42 pKa = 4.15 NFVYY46 pKa = 10.56 FDD48 pKa = 4.21 SNRR51 pKa = 11.84 SKK53 pKa = 11.1 CGFEE57 pKa = 4.74 KK58 pKa = 10.31 EE59 pKa = 4.04 DD60 pKa = 3.84 HH61 pKa = 6.3 VLILQIIPLLRR72 pKa = 11.84 II73 pKa = 3.69
Molecular weight: 8.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.561
IPC2_protein 5.728
IPC_protein 5.601
Toseland 5.728
ProMoST 5.792
Dawson 5.677
Bjellqvist 5.728
Wikipedia 5.601
Rodwell 5.639
Grimsley 5.741
Solomon 5.677
Lehninger 5.652
Nozaki 5.855
DTASelect 5.995
Thurlkill 5.868
EMBOSS 5.817
Sillero 5.957
Patrickios 4.444
IPC_peptide 5.703
IPC2_peptide 5.944
IPC2.peptide.svr19 6.152
Protein with the highest isoelectric point:
>tr|Q8JJW6|Q8JJW6_9CLOS p22 protein OS=Sweet potato chlorotic stunt virus OX=81931 GN=p22 PE=4 SV=1
MM1 pKa = 6.45 TQSIRR6 pKa = 11.84 EE7 pKa = 4.16 SMGLCNPRR15 pKa = 11.84 TRR17 pKa = 11.84 ISVTYY22 pKa = 9.68 WMQSDD27 pKa = 4.29 CPSYY31 pKa = 11.34 LLLFCVYY38 pKa = 10.22 KK39 pKa = 10.9 SKK41 pKa = 7.76 TQKK44 pKa = 10.96 YY45 pKa = 8.95 IFSPP49 pKa = 3.71
Molecular weight: 5.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.844
IPC2_protein 8.536
IPC_protein 8.682
Toseland 8.726
ProMoST 8.916
Dawson 9.121
Bjellqvist 9.253
Wikipedia 9.209
Rodwell 9.151
Grimsley 8.668
Solomon 9.385
Lehninger 9.355
Nozaki 9.37
DTASelect 9.063
Thurlkill 9.092
EMBOSS 9.268
Sillero 9.355
Patrickios 2.282
IPC_peptide 9.355
IPC2_peptide 8.536
IPC2.peptide.svr19 8.123
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
11
0
11
5346
49
2492
486.0
55.68
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.34 ± 0.466
1.945 ± 0.265
6.828 ± 0.194
5.275 ± 0.307
6.117 ± 0.245
4.564 ± 0.28
1.871 ± 0.237
5.593 ± 0.474
7.632 ± 0.568
9.802 ± 0.518
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.825 ± 0.201
5.986 ± 0.57
3.049 ± 0.249
2.376 ± 0.182
5.238 ± 0.17
8.511 ± 0.65
5.238 ± 0.22
7.987 ± 0.528
0.767 ± 0.179
4.059 ± 0.303
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here