Amino acid dipepetide frequency for Phage AS32

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.201AlaAla: 9.201 ± 1.445
0.669AlaCys: 0.669 ± 0.282
5.102AlaAsp: 5.102 ± 0.555
4.182AlaGlu: 4.182 ± 0.628
2.677AlaPhe: 2.677 ± 0.473
8.03AlaGly: 8.03 ± 0.966
0.669AlaHis: 0.669 ± 0.197
5.353AlaIle: 5.353 ± 0.78
4.35AlaLys: 4.35 ± 0.815
6.692AlaLeu: 6.692 ± 1.244
2.928AlaMet: 2.928 ± 0.652
4.601AlaAsn: 4.601 ± 0.717
4.099AlaPro: 4.099 ± 0.774
3.848AlaGln: 3.848 ± 0.583
5.27AlaArg: 5.27 ± 0.681
6.19AlaSer: 6.19 ± 0.948
5.688AlaThr: 5.688 ± 1.053
7.779AlaVal: 7.779 ± 0.859
1.924AlaTrp: 1.924 ± 0.581
3.346AlaTyr: 3.346 ± 0.588
0.0AlaXaa: 0.0 ± 0.0
Cys
0.586CysAla: 0.586 ± 0.229
0.084CysCys: 0.084 ± 0.084
0.335CysAsp: 0.335 ± 0.185
0.753CysGlu: 0.753 ± 0.273
0.0CysPhe: 0.0 ± 0.0
0.418CysGly: 0.418 ± 0.224
0.084CysHis: 0.084 ± 0.077
0.418CysIle: 0.418 ± 0.184
0.335CysLys: 0.335 ± 0.183
0.335CysLeu: 0.335 ± 0.172
0.0CysMet: 0.0 ± 0.0
0.335CysAsn: 0.335 ± 0.185
0.084CysPro: 0.084 ± 0.095
0.335CysGln: 0.335 ± 0.155
0.084CysArg: 0.084 ± 0.081
0.586CysSer: 0.586 ± 0.291
0.418CysThr: 0.418 ± 0.214
0.167CysVal: 0.167 ± 0.12
0.084CysTrp: 0.084 ± 0.084
0.251CysTyr: 0.251 ± 0.132
0.0CysXaa: 0.0 ± 0.0
Asp
5.27AspAla: 5.27 ± 0.548
0.836AspCys: 0.836 ± 0.319
3.095AspAsp: 3.095 ± 0.525
4.099AspGlu: 4.099 ± 0.75
1.924AspPhe: 1.924 ± 0.482
4.768AspGly: 4.768 ± 0.759
1.004AspHis: 1.004 ± 0.333
3.597AspIle: 3.597 ± 0.386
3.179AspLys: 3.179 ± 0.529
5.186AspLeu: 5.186 ± 0.68
1.506AspMet: 1.506 ± 0.33
2.426AspAsn: 2.426 ± 0.463
4.099AspPro: 4.099 ± 0.572
1.589AspGln: 1.589 ± 0.413
2.928AspArg: 2.928 ± 0.619
4.182AspSer: 4.182 ± 0.872
3.764AspThr: 3.764 ± 0.501
4.517AspVal: 4.517 ± 0.649
1.757AspTrp: 1.757 ± 0.374
1.84AspTyr: 1.84 ± 0.36
0.0AspXaa: 0.0 ± 0.0
Glu
6.524GluAla: 6.524 ± 0.875
0.418GluCys: 0.418 ± 0.204
3.848GluAsp: 3.848 ± 0.482
5.353GluGlu: 5.353 ± 0.812
2.76GluPhe: 2.76 ± 0.436
4.015GluGly: 4.015 ± 0.809
0.586GluHis: 0.586 ± 0.203
4.266GluIle: 4.266 ± 0.619
4.601GluLys: 4.601 ± 0.893
5.521GluLeu: 5.521 ± 0.759
1.673GluMet: 1.673 ± 0.326
3.011GluAsn: 3.011 ± 0.54
2.593GluPro: 2.593 ± 0.872
3.179GluGln: 3.179 ± 0.393
3.262GluArg: 3.262 ± 0.597
4.015GluSer: 4.015 ± 0.537
3.513GluThr: 3.513 ± 0.544
4.35GluVal: 4.35 ± 0.567
1.004GluTrp: 1.004 ± 0.277
1.84GluTyr: 1.84 ± 0.386
0.0GluXaa: 0.0 ± 0.0
Phe
3.848PheAla: 3.848 ± 0.616
0.167PheCys: 0.167 ± 0.123
2.76PheAsp: 2.76 ± 0.56
2.509PheGlu: 2.509 ± 0.405
1.589PhePhe: 1.589 ± 0.331
2.593PheGly: 2.593 ± 0.52
0.418PheHis: 0.418 ± 0.232
1.506PheIle: 1.506 ± 0.339
2.342PheLys: 2.342 ± 0.504
2.175PheLeu: 2.175 ± 0.348
0.753PheMet: 0.753 ± 0.196
1.589PheAsn: 1.589 ± 0.291
1.589PhePro: 1.589 ± 0.362
1.84PheGln: 1.84 ± 0.363
2.258PheArg: 2.258 ± 0.5
1.924PheSer: 1.924 ± 0.404
3.011PheThr: 3.011 ± 0.416
1.924PheVal: 1.924 ± 0.49
0.669PheTrp: 0.669 ± 0.2
1.506PheTyr: 1.506 ± 0.309
0.0PheXaa: 0.0 ± 0.0
Gly
7.194GlyAla: 7.194 ± 0.669
0.418GlyCys: 0.418 ± 0.21
6.106GlyAsp: 6.106 ± 0.676
5.27GlyGlu: 5.27 ± 1.051
4.099GlyPhe: 4.099 ± 0.525
6.023GlyGly: 6.023 ± 1.002
0.753GlyHis: 0.753 ± 0.248
4.266GlyIle: 4.266 ± 0.648
3.597GlyLys: 3.597 ± 0.454
6.357GlyLeu: 6.357 ± 0.899
1.84GlyMet: 1.84 ± 0.492
3.513GlyAsn: 3.513 ± 0.776
4.433GlyPro: 4.433 ± 0.902
2.175GlyGln: 2.175 ± 0.447
2.844GlyArg: 2.844 ± 0.536
5.855GlySer: 5.855 ± 0.91
6.274GlyThr: 6.274 ± 1.165
5.855GlyVal: 5.855 ± 0.824
1.757GlyTrp: 1.757 ± 0.323
1.673GlyTyr: 1.673 ± 0.505
0.0GlyXaa: 0.0 ± 0.0
His
0.753HisAla: 0.753 ± 0.268
0.084HisCys: 0.084 ± 0.084
0.753HisAsp: 0.753 ± 0.256
0.753HisGlu: 0.753 ± 0.327
0.669HisPhe: 0.669 ± 0.225
0.669HisGly: 0.669 ± 0.31
0.335HisHis: 0.335 ± 0.174
0.836HisIle: 0.836 ± 0.283
0.669HisLys: 0.669 ± 0.195
1.338HisLeu: 1.338 ± 0.261
0.669HisMet: 0.669 ± 0.189
0.836HisAsn: 0.836 ± 0.328
0.753HisPro: 0.753 ± 0.288
0.418HisGln: 0.418 ± 0.188
0.92HisArg: 0.92 ± 0.323
1.087HisSer: 1.087 ± 0.312
0.92HisThr: 0.92 ± 0.313
0.753HisVal: 0.753 ± 0.202
0.335HisTrp: 0.335 ± 0.17
0.836HisTyr: 0.836 ± 0.257
0.0HisXaa: 0.0 ± 0.0
Ile
6.023IleAla: 6.023 ± 0.853
0.167IleCys: 0.167 ± 0.106
4.266IleAsp: 4.266 ± 0.717
4.266IleGlu: 4.266 ± 0.591
1.171IlePhe: 1.171 ± 0.26
4.182IleGly: 4.182 ± 0.6
1.171IleHis: 1.171 ± 0.302
2.76IleIle: 2.76 ± 0.549
2.677IleLys: 2.677 ± 0.485
3.848IleLeu: 3.848 ± 0.496
1.087IleMet: 1.087 ± 0.272
2.175IleAsn: 2.175 ± 0.338
2.593IlePro: 2.593 ± 0.415
2.258IleGln: 2.258 ± 0.57
3.262IleArg: 3.262 ± 0.504
3.179IleSer: 3.179 ± 0.506
4.099IleThr: 4.099 ± 0.607
4.35IleVal: 4.35 ± 0.468
1.338IleTrp: 1.338 ± 0.404
1.84IleTyr: 1.84 ± 0.519
0.0IleXaa: 0.0 ± 0.0
Lys
5.521LysAla: 5.521 ± 0.815
0.167LysCys: 0.167 ± 0.108
2.426LysAsp: 2.426 ± 0.508
3.764LysGlu: 3.764 ± 0.6
1.673LysPhe: 1.673 ± 0.351
3.848LysGly: 3.848 ± 0.702
0.669LysHis: 0.669 ± 0.323
3.346LysIle: 3.346 ± 0.441
3.764LysLys: 3.764 ± 0.684
3.764LysLeu: 3.764 ± 0.885
1.506LysMet: 1.506 ± 0.366
1.422LysAsn: 1.422 ± 0.295
2.593LysPro: 2.593 ± 0.473
2.175LysGln: 2.175 ± 0.428
1.924LysArg: 1.924 ± 0.546
2.426LysSer: 2.426 ± 0.52
3.179LysThr: 3.179 ± 0.474
4.266LysVal: 4.266 ± 0.604
1.087LysTrp: 1.087 ± 0.375
1.422LysTyr: 1.422 ± 0.34
0.0LysXaa: 0.0 ± 0.0
Leu
6.357LeuAla: 6.357 ± 1.004
0.084LeuCys: 0.084 ± 0.077
4.35LeuAsp: 4.35 ± 0.451
6.608LeuGlu: 6.608 ± 0.736
3.011LeuPhe: 3.011 ± 0.42
6.692LeuGly: 6.692 ± 0.804
0.92LeuHis: 0.92 ± 0.284
3.848LeuIle: 3.848 ± 0.443
3.43LeuLys: 3.43 ± 0.699
6.023LeuLeu: 6.023 ± 0.819
2.593LeuMet: 2.593 ± 0.579
3.764LeuAsn: 3.764 ± 0.497
3.764LeuPro: 3.764 ± 0.418
3.095LeuGln: 3.095 ± 0.73
5.186LeuArg: 5.186 ± 0.74
4.601LeuSer: 4.601 ± 0.594
4.266LeuThr: 4.266 ± 0.63
5.27LeuVal: 5.27 ± 0.777
1.087LeuTrp: 1.087 ± 0.466
1.589LeuTyr: 1.589 ± 0.373
0.0LeuXaa: 0.0 ± 0.0
Met
2.175MetAla: 2.175 ± 0.574
0.418MetCys: 0.418 ± 0.183
0.92MetAsp: 0.92 ± 0.265
1.506MetGlu: 1.506 ± 0.469
1.255MetPhe: 1.255 ± 0.384
2.426MetGly: 2.426 ± 0.471
0.669MetHis: 0.669 ± 0.229
1.087MetIle: 1.087 ± 0.367
1.087MetLys: 1.087 ± 0.341
2.091MetLeu: 2.091 ± 0.438
0.418MetMet: 0.418 ± 0.21
0.753MetAsn: 0.753 ± 0.233
1.171MetPro: 1.171 ± 0.419
1.004MetGln: 1.004 ± 0.304
1.422MetArg: 1.422 ± 0.477
2.677MetSer: 2.677 ± 0.573
2.091MetThr: 2.091 ± 0.494
0.669MetVal: 0.669 ± 0.205
0.335MetTrp: 0.335 ± 0.144
0.167MetTyr: 0.167 ± 0.108
0.0MetXaa: 0.0 ± 0.0
Asn
4.266AsnAla: 4.266 ± 0.702
0.335AsnCys: 0.335 ± 0.159
1.84AsnAsp: 1.84 ± 0.3
2.928AsnGlu: 2.928 ± 0.48
2.091AsnPhe: 2.091 ± 0.459
3.764AsnGly: 3.764 ± 0.567
1.255AsnHis: 1.255 ± 0.255
2.677AsnIle: 2.677 ± 0.387
1.924AsnLys: 1.924 ± 0.432
3.848AsnLeu: 3.848 ± 0.561
0.669AsnMet: 0.669 ± 0.2
1.757AsnAsn: 1.757 ± 0.377
2.342AsnPro: 2.342 ± 0.547
2.175AsnGln: 2.175 ± 0.454
2.677AsnArg: 2.677 ± 0.39
2.342AsnSer: 2.342 ± 0.326
2.677AsnThr: 2.677 ± 0.533
2.509AsnVal: 2.509 ± 0.501
1.004AsnTrp: 1.004 ± 0.215
2.008AsnTyr: 2.008 ± 0.428
0.0AsnXaa: 0.0 ± 0.0
Pro
4.684ProAla: 4.684 ± 1.062
0.418ProCys: 0.418 ± 0.217
2.008ProAsp: 2.008 ± 0.469
4.182ProGlu: 4.182 ± 0.69
1.506ProPhe: 1.506 ± 0.35
4.433ProGly: 4.433 ± 0.927
0.92ProHis: 0.92 ± 0.198
2.593ProIle: 2.593 ± 0.472
2.509ProLys: 2.509 ± 0.475
3.43ProLeu: 3.43 ± 0.483
0.502ProMet: 0.502 ± 0.219
1.924ProAsn: 1.924 ± 0.386
2.091ProPro: 2.091 ± 0.336
1.087ProGln: 1.087 ± 0.325
1.673ProArg: 1.673 ± 0.419
3.262ProSer: 3.262 ± 0.704
3.43ProThr: 3.43 ± 0.502
3.931ProVal: 3.931 ± 0.662
1.004ProTrp: 1.004 ± 0.269
1.338ProTyr: 1.338 ± 0.345
0.0ProXaa: 0.0 ± 0.0
Gln
3.262GlnAla: 3.262 ± 0.485
0.0GlnCys: 0.0 ± 0.0
1.757GlnAsp: 1.757 ± 0.363
2.677GlnGlu: 2.677 ± 0.543
1.087GlnPhe: 1.087 ± 0.262
3.513GlnGly: 3.513 ± 0.708
0.586GlnHis: 0.586 ± 0.215
2.593GlnIle: 2.593 ± 0.522
2.258GlnLys: 2.258 ± 0.421
3.179GlnLeu: 3.179 ± 0.558
1.004GlnMet: 1.004 ± 0.361
1.255GlnAsn: 1.255 ± 0.336
1.255GlnPro: 1.255 ± 0.327
1.589GlnGln: 1.589 ± 0.347
2.091GlnArg: 2.091 ± 0.39
2.008GlnSer: 2.008 ± 0.502
2.008GlnThr: 2.008 ± 0.382
3.179GlnVal: 3.179 ± 0.576
0.836GlnTrp: 0.836 ± 0.318
1.589GlnTyr: 1.589 ± 0.36
0.0GlnXaa: 0.0 ± 0.0
Arg
3.513ArgAla: 3.513 ± 0.733
0.084ArgCys: 0.084 ± 0.086
4.015ArgAsp: 4.015 ± 0.486
3.513ArgGlu: 3.513 ± 0.505
1.757ArgPhe: 1.757 ± 0.393
4.182ArgGly: 4.182 ± 0.527
1.087ArgHis: 1.087 ± 0.369
4.099ArgIle: 4.099 ± 0.616
3.43ArgLys: 3.43 ± 0.673
3.848ArgLeu: 3.848 ± 0.572
1.422ArgMet: 1.422 ± 0.246
2.509ArgAsn: 2.509 ± 0.481
2.008ArgPro: 2.008 ± 0.509
1.589ArgGln: 1.589 ± 0.358
3.346ArgArg: 3.346 ± 0.489
2.76ArgSer: 2.76 ± 0.468
2.091ArgThr: 2.091 ± 0.519
3.011ArgVal: 3.011 ± 0.517
1.255ArgTrp: 1.255 ± 0.321
1.255ArgTyr: 1.255 ± 0.276
0.0ArgXaa: 0.0 ± 0.0
Ser
5.102SerAla: 5.102 ± 0.811
0.167SerCys: 0.167 ± 0.122
5.019SerAsp: 5.019 ± 0.727
3.179SerGlu: 3.179 ± 0.592
3.262SerPhe: 3.262 ± 0.529
6.274SerGly: 6.274 ± 1.135
0.753SerHis: 0.753 ± 0.312
3.43SerIle: 3.43 ± 0.703
2.677SerLys: 2.677 ± 0.482
4.35SerLeu: 4.35 ± 0.49
1.589SerMet: 1.589 ± 0.285
3.43SerAsn: 3.43 ± 0.797
3.262SerPro: 3.262 ± 0.545
2.426SerGln: 2.426 ± 0.56
2.677SerArg: 2.677 ± 0.504
5.604SerSer: 5.604 ± 0.842
4.768SerThr: 4.768 ± 0.953
4.768SerVal: 4.768 ± 0.488
0.586SerTrp: 0.586 ± 0.202
2.342SerTyr: 2.342 ± 0.63
0.0SerXaa: 0.0 ± 0.0
Thr
6.859ThrAla: 6.859 ± 1.287
0.251ThrCys: 0.251 ± 0.148
4.099ThrAsp: 4.099 ± 0.662
2.509ThrGlu: 2.509 ± 0.358
2.593ThrPhe: 2.593 ± 0.403
5.604ThrGly: 5.604 ± 1.026
1.087ThrHis: 1.087 ± 0.262
3.43ThrIle: 3.43 ± 0.467
2.342ThrLys: 2.342 ± 0.309
5.019ThrLeu: 5.019 ± 0.593
1.255ThrMet: 1.255 ± 0.247
2.928ThrAsn: 2.928 ± 0.422
3.513ThrPro: 3.513 ± 0.641
2.426ThrGln: 2.426 ± 0.52
2.509ThrArg: 2.509 ± 0.495
4.601ThrSer: 4.601 ± 0.89
4.015ThrThr: 4.015 ± 0.609
6.943ThrVal: 6.943 ± 0.932
0.836ThrTrp: 0.836 ± 0.244
2.426ThrTyr: 2.426 ± 0.39
0.0ThrXaa: 0.0 ± 0.0
Val
7.361ValAla: 7.361 ± 0.818
0.753ValCys: 0.753 ± 0.295
4.433ValAsp: 4.433 ± 0.662
5.186ValGlu: 5.186 ± 0.722
1.84ValPhe: 1.84 ± 0.331
4.684ValGly: 4.684 ± 0.447
1.004ValHis: 1.004 ± 0.245
3.43ValIle: 3.43 ± 0.549
4.35ValLys: 4.35 ± 0.696
5.27ValLeu: 5.27 ± 0.604
1.757ValMet: 1.757 ± 0.423
3.68ValAsn: 3.68 ± 0.431
3.262ValPro: 3.262 ± 0.521
2.593ValGln: 2.593 ± 0.432
3.346ValArg: 3.346 ± 0.555
4.601ValSer: 4.601 ± 0.901
5.939ValThr: 5.939 ± 0.892
4.601ValVal: 4.601 ± 0.584
1.924ValTrp: 1.924 ± 0.5
1.84ValTyr: 1.84 ± 0.384
0.0ValXaa: 0.0 ± 0.0
Trp
1.757TrpAla: 1.757 ± 0.37
0.167TrpCys: 0.167 ± 0.122
1.924TrpAsp: 1.924 ± 0.384
1.255TrpGlu: 1.255 ± 0.277
1.338TrpPhe: 1.338 ± 0.386
1.673TrpGly: 1.673 ± 0.435
0.167TrpHis: 0.167 ± 0.134
0.753TrpIle: 0.753 ± 0.269
0.586TrpLys: 0.586 ± 0.363
1.338TrpLeu: 1.338 ± 0.312
0.418TrpMet: 0.418 ± 0.183
1.338TrpAsn: 1.338 ± 0.572
0.418TrpPro: 0.418 ± 0.164
0.753TrpGln: 0.753 ± 0.276
1.004TrpArg: 1.004 ± 0.317
1.338TrpSer: 1.338 ± 0.31
1.171TrpThr: 1.171 ± 0.351
1.338TrpVal: 1.338 ± 0.26
0.167TrpTrp: 0.167 ± 0.131
0.836TrpTyr: 0.836 ± 0.298
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.342TyrAla: 2.342 ± 0.452
0.0TyrCys: 0.0 ± 0.0
2.509TyrAsp: 2.509 ± 0.49
1.84TyrGlu: 1.84 ± 0.362
0.669TyrPhe: 0.669 ± 0.187
2.509TyrGly: 2.509 ± 0.401
0.251TyrHis: 0.251 ± 0.125
2.426TyrIle: 2.426 ± 0.508
0.836TyrLys: 0.836 ± 0.306
2.928TyrLeu: 2.928 ± 0.517
0.669TyrMet: 0.669 ± 0.213
1.84TyrAsn: 1.84 ± 0.278
1.087TyrPro: 1.087 ± 0.389
1.171TyrGln: 1.171 ± 0.447
2.091TyrArg: 2.091 ± 0.48
2.426TyrSer: 2.426 ± 0.653
1.924TyrThr: 1.924 ± 0.396
1.673TyrVal: 1.673 ± 0.351
0.753TyrTrp: 0.753 ± 0.239
0.669TyrTyr: 0.669 ± 0.202
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 45 proteins (11956 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski