Amino acid dipepetide frequency for Staphylococcus phage phi13 (Bacteriophage phi-13)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
1.979AlaAla: 1.979 ± 1.026
0.45AlaCys: 0.45 ± 0.219
2.159AlaAsp: 2.159 ± 0.437
2.609AlaGlu: 2.609 ± 0.652
2.339AlaPhe: 2.339 ± 0.65
3.958AlaGly: 3.958 ± 0.752
0.63AlaHis: 0.63 ± 0.281
5.577AlaIle: 5.577 ± 0.788
4.498AlaLys: 4.498 ± 0.564
5.487AlaLeu: 5.487 ± 0.826
1.439AlaMet: 1.439 ± 0.384
2.699AlaAsn: 2.699 ± 0.384
1.259AlaPro: 1.259 ± 0.284
2.699AlaGln: 2.699 ± 0.547
3.058AlaArg: 3.058 ± 0.436
3.418AlaSer: 3.418 ± 0.631
4.048AlaThr: 4.048 ± 0.661
3.598AlaVal: 3.598 ± 0.501
0.63AlaTrp: 0.63 ± 0.315
2.159AlaTyr: 2.159 ± 0.41
0.0AlaXaa: 0.0 ± 0.0
Cys
0.36CysAla: 0.36 ± 0.164
0.0CysCys: 0.0 ± 0.0
0.18CysAsp: 0.18 ± 0.223
0.45CysGlu: 0.45 ± 0.252
0.27CysPhe: 0.27 ± 0.174
0.45CysGly: 0.45 ± 0.304
0.27CysHis: 0.27 ± 0.184
0.72CysIle: 0.72 ± 0.252
0.54CysLys: 0.54 ± 0.23
0.36CysLeu: 0.36 ± 0.161
0.0CysMet: 0.0 ± 0.0
0.18CysAsn: 0.18 ± 0.138
0.0CysPro: 0.0 ± 0.0
0.09CysGln: 0.09 ± 0.113
0.54CysArg: 0.54 ± 0.306
0.36CysSer: 0.36 ± 0.2
0.45CysThr: 0.45 ± 0.184
0.36CysVal: 0.36 ± 0.226
0.0CysTrp: 0.0 ± 0.0
0.54CysTyr: 0.54 ± 0.228
0.0CysXaa: 0.0 ± 0.0
Asp
2.878AspAla: 2.878 ± 0.525
0.36AspCys: 0.36 ± 0.216
3.778AspAsp: 3.778 ± 0.669
5.667AspGlu: 5.667 ± 0.802
3.598AspPhe: 3.598 ± 0.708
4.947AspGly: 4.947 ± 0.726
0.72AspHis: 0.72 ± 0.252
4.857AspIle: 4.857 ± 0.742
5.937AspLys: 5.937 ± 0.768
5.127AspLeu: 5.127 ± 0.708
1.709AspMet: 1.709 ± 0.377
3.418AspAsn: 3.418 ± 0.56
1.439AspPro: 1.439 ± 0.555
1.259AspGln: 1.259 ± 0.329
2.429AspArg: 2.429 ± 0.578
3.328AspSer: 3.328 ± 0.557
3.688AspThr: 3.688 ± 0.783
4.138AspVal: 4.138 ± 0.791
0.45AspTrp: 0.45 ± 0.258
2.699AspTyr: 2.699 ± 0.386
0.0AspXaa: 0.0 ± 0.0
Glu
3.958GluAla: 3.958 ± 0.811
0.54GluCys: 0.54 ± 0.206
3.598GluAsp: 3.598 ± 0.539
5.487GluGlu: 5.487 ± 0.791
3.508GluPhe: 3.508 ± 0.626
3.148GluGly: 3.148 ± 0.58
1.529GluHis: 1.529 ± 0.398
5.757GluIle: 5.757 ± 1.023
6.387GluLys: 6.387 ± 0.976
7.556GluLeu: 7.556 ± 1.159
2.519GluMet: 2.519 ± 0.49
4.678GluAsn: 4.678 ± 0.682
1.799GluPro: 1.799 ± 0.364
3.688GluGln: 3.688 ± 0.695
4.048GluArg: 4.048 ± 0.639
4.048GluSer: 4.048 ± 0.6
3.508GluThr: 3.508 ± 0.651
4.228GluVal: 4.228 ± 0.8
1.079GluTrp: 1.079 ± 0.324
3.958GluTyr: 3.958 ± 0.931
0.0GluXaa: 0.0 ± 0.0
Phe
2.339PheAla: 2.339 ± 0.471
0.36PheCys: 0.36 ± 0.189
2.789PheAsp: 2.789 ± 0.524
2.339PheGlu: 2.339 ± 0.436
0.81PhePhe: 0.81 ± 0.246
2.789PheGly: 2.789 ± 0.556
0.9PheHis: 0.9 ± 0.317
3.958PheIle: 3.958 ± 0.792
4.408PheLys: 4.408 ± 0.606
2.519PheLeu: 2.519 ± 0.608
1.169PheMet: 1.169 ± 0.335
3.868PheAsn: 3.868 ± 0.576
0.81PhePro: 0.81 ± 0.324
0.989PheGln: 0.989 ± 0.303
1.889PheArg: 1.889 ± 0.302
2.609PheSer: 2.609 ± 0.539
2.699PheThr: 2.699 ± 0.614
1.799PheVal: 1.799 ± 0.377
0.18PheTrp: 0.18 ± 0.121
1.619PheTyr: 1.619 ± 0.416
0.0PheXaa: 0.0 ± 0.0
Gly
3.328GlyAla: 3.328 ± 0.879
0.36GlyCys: 0.36 ± 0.187
2.878GlyAsp: 2.878 ± 0.535
3.328GlyGlu: 3.328 ± 0.598
2.789GlyPhe: 2.789 ± 0.615
3.058GlyGly: 3.058 ± 0.947
1.079GlyHis: 1.079 ± 0.282
4.767GlyIle: 4.767 ± 0.953
7.106GlyLys: 7.106 ± 0.892
5.847GlyLeu: 5.847 ± 1.02
1.079GlyMet: 1.079 ± 0.325
3.418GlyAsn: 3.418 ± 0.565
0.9GlyPro: 0.9 ± 0.375
2.159GlyGln: 2.159 ± 0.491
2.159GlyArg: 2.159 ± 0.495
3.508GlySer: 3.508 ± 0.502
3.868GlyThr: 3.868 ± 0.46
3.148GlyVal: 3.148 ± 0.685
0.989GlyTrp: 0.989 ± 0.358
3.238GlyTyr: 3.238 ± 0.476
0.0GlyXaa: 0.0 ± 0.0
His
0.989HisAla: 0.989 ± 0.39
0.18HisCys: 0.18 ± 0.142
0.81HisAsp: 0.81 ± 0.323
1.259HisGlu: 1.259 ± 0.277
1.169HisPhe: 1.169 ± 0.263
0.63HisGly: 0.63 ± 0.344
0.27HisHis: 0.27 ± 0.252
1.979HisIle: 1.979 ± 0.476
1.259HisLys: 1.259 ± 0.354
1.529HisLeu: 1.529 ± 0.3
0.27HisMet: 0.27 ± 0.164
1.169HisAsn: 1.169 ± 0.386
0.54HisPro: 0.54 ± 0.263
0.45HisGln: 0.45 ± 0.183
0.72HisArg: 0.72 ± 0.228
1.169HisSer: 1.169 ± 0.284
1.079HisThr: 1.079 ± 0.37
0.72HisVal: 0.72 ± 0.288
0.27HisTrp: 0.27 ± 0.153
0.9HisTyr: 0.9 ± 0.333
0.0HisXaa: 0.0 ± 0.0
Ile
4.767IleAla: 4.767 ± 0.59
0.54IleCys: 0.54 ± 0.231
5.577IleAsp: 5.577 ± 0.615
6.567IleGlu: 6.567 ± 1.105
2.968IlePhe: 2.968 ± 0.637
4.138IleGly: 4.138 ± 0.556
1.169IleHis: 1.169 ± 0.374
5.037IleIle: 5.037 ± 0.764
7.646IleLys: 7.646 ± 0.842
4.767IleLeu: 4.767 ± 0.683
2.069IleMet: 2.069 ± 0.485
5.937IleAsn: 5.937 ± 0.859
1.979IlePro: 1.979 ± 0.408
3.238IleGln: 3.238 ± 0.604
3.238IleArg: 3.238 ± 0.672
4.408IleSer: 4.408 ± 0.627
4.857IleThr: 4.857 ± 0.499
4.857IleVal: 4.857 ± 0.512
1.259IleTrp: 1.259 ± 0.528
2.789IleTyr: 2.789 ± 0.547
0.0IleXaa: 0.0 ± 0.0
Lys
6.297LysAla: 6.297 ± 0.578
0.27LysCys: 0.27 ± 0.218
5.217LysAsp: 5.217 ± 0.506
8.006LysGlu: 8.006 ± 1.099
4.588LysPhe: 4.588 ± 0.726
6.656LysGly: 6.656 ± 0.896
2.159LysHis: 2.159 ± 0.578
6.297LysIle: 6.297 ± 0.688
7.106LysLys: 7.106 ± 0.85
7.826LysLeu: 7.826 ± 1.007
2.069LysMet: 2.069 ± 0.424
4.228LysAsn: 4.228 ± 0.67
3.418LysPro: 3.418 ± 0.696
4.138LysGln: 4.138 ± 0.665
4.138LysArg: 4.138 ± 0.595
5.487LysSer: 5.487 ± 0.671
5.667LysThr: 5.667 ± 0.93
6.027LysVal: 6.027 ± 0.728
1.079LysTrp: 1.079 ± 0.382
3.958LysTyr: 3.958 ± 0.564
0.0LysXaa: 0.0 ± 0.0
Leu
4.318LeuAla: 4.318 ± 0.633
0.54LeuCys: 0.54 ± 0.232
5.757LeuAsp: 5.757 ± 0.834
7.016LeuGlu: 7.016 ± 1.155
3.058LeuPhe: 3.058 ± 0.467
4.228LeuGly: 4.228 ± 0.886
0.989LeuHis: 0.989 ± 0.279
5.307LeuIle: 5.307 ± 0.679
8.096LeuLys: 8.096 ± 1.065
6.207LeuLeu: 6.207 ± 1.05
1.349LeuMet: 1.349 ± 0.402
6.027LeuAsn: 6.027 ± 0.687
2.159LeuPro: 2.159 ± 0.489
3.058LeuGln: 3.058 ± 0.475
4.048LeuArg: 4.048 ± 0.553
5.937LeuSer: 5.937 ± 0.747
4.138LeuThr: 4.138 ± 0.552
4.588LeuVal: 4.588 ± 0.596
0.81LeuTrp: 0.81 ± 0.25
2.878LeuTyr: 2.878 ± 0.453
0.0LeuXaa: 0.0 ± 0.0
Met
1.259MetAla: 1.259 ± 0.358
0.18MetCys: 0.18 ± 0.138
1.169MetAsp: 1.169 ± 0.27
1.709MetGlu: 1.709 ± 0.403
1.079MetPhe: 1.079 ± 0.276
0.9MetGly: 0.9 ± 0.422
0.36MetHis: 0.36 ± 0.202
1.619MetIle: 1.619 ± 0.322
2.878MetLys: 2.878 ± 0.547
1.799MetLeu: 1.799 ± 0.375
0.81MetMet: 0.81 ± 0.27
1.709MetAsn: 1.709 ± 0.475
1.259MetPro: 1.259 ± 0.425
0.81MetGln: 0.81 ± 0.305
1.349MetArg: 1.349 ± 0.466
2.159MetSer: 2.159 ± 0.331
1.349MetThr: 1.349 ± 0.288
1.349MetVal: 1.349 ± 0.322
0.45MetTrp: 0.45 ± 0.182
0.9MetTyr: 0.9 ± 0.27
0.0MetXaa: 0.0 ± 0.0
Asn
3.508AsnAla: 3.508 ± 0.632
0.18AsnCys: 0.18 ± 0.12
3.868AsnAsp: 3.868 ± 0.654
5.217AsnGlu: 5.217 ± 0.784
1.709AsnPhe: 1.709 ± 0.377
5.397AsnGly: 5.397 ± 0.858
0.81AsnHis: 0.81 ± 0.225
5.217AsnIle: 5.217 ± 0.706
5.667AsnLys: 5.667 ± 0.787
5.307AsnLeu: 5.307 ± 0.732
1.799AsnMet: 1.799 ± 0.316
4.947AsnAsn: 4.947 ± 0.792
2.519AsnPro: 2.519 ± 0.342
3.058AsnGln: 3.058 ± 0.51
2.699AsnArg: 2.699 ± 0.424
2.339AsnSer: 2.339 ± 0.49
3.868AsnThr: 3.868 ± 0.507
3.598AsnVal: 3.598 ± 0.608
1.259AsnTrp: 1.259 ± 0.433
2.339AsnTyr: 2.339 ± 0.455
0.0AsnXaa: 0.0 ± 0.0
Pro
0.9ProAla: 0.9 ± 0.332
0.0ProCys: 0.0 ± 0.0
1.619ProAsp: 1.619 ± 0.486
1.349ProGlu: 1.349 ± 0.323
0.63ProPhe: 0.63 ± 0.226
1.079ProGly: 1.079 ± 0.293
0.9ProHis: 0.9 ± 0.295
2.069ProIle: 2.069 ± 0.432
2.519ProLys: 2.519 ± 0.67
2.249ProLeu: 2.249 ± 0.605
0.989ProMet: 0.989 ± 0.323
1.619ProAsn: 1.619 ± 0.397
0.9ProPro: 0.9 ± 0.257
0.9ProGln: 0.9 ± 0.254
0.9ProArg: 0.9 ± 0.256
1.889ProSer: 1.889 ± 0.537
2.069ProThr: 2.069 ± 0.507
1.709ProVal: 1.709 ± 0.448
0.18ProTrp: 0.18 ± 0.113
1.349ProTyr: 1.349 ± 0.383
0.0ProXaa: 0.0 ± 0.0
Gln
3.508GlnAla: 3.508 ± 0.503
0.36GlnCys: 0.36 ± 0.204
2.789GlnAsp: 2.789 ± 0.561
3.058GlnGlu: 3.058 ± 0.701
1.079GlnPhe: 1.079 ± 0.336
1.259GlnGly: 1.259 ± 0.341
0.9GlnHis: 0.9 ± 0.28
2.519GlnIle: 2.519 ± 0.415
3.508GlnLys: 3.508 ± 0.662
3.418GlnLeu: 3.418 ± 0.58
0.9GlnMet: 0.9 ± 0.313
2.429GlnAsn: 2.429 ± 0.521
0.989GlnPro: 0.989 ± 0.339
1.439GlnGln: 1.439 ± 0.37
1.799GlnArg: 1.799 ± 0.381
2.069GlnSer: 2.069 ± 0.564
1.709GlnThr: 1.709 ± 0.36
1.889GlnVal: 1.889 ± 0.546
0.18GlnTrp: 0.18 ± 0.166
2.519GlnTyr: 2.519 ± 0.479
0.0GlnXaa: 0.0 ± 0.0
Arg
2.069ArgAla: 2.069 ± 0.478
0.45ArgCys: 0.45 ± 0.277
3.688ArgAsp: 3.688 ± 0.594
4.588ArgGlu: 4.588 ± 0.625
1.889ArgPhe: 1.889 ± 0.415
1.889ArgGly: 1.889 ± 0.446
0.989ArgHis: 0.989 ± 0.267
3.058ArgIle: 3.058 ± 0.5
3.508ArgLys: 3.508 ± 0.673
3.328ArgLeu: 3.328 ± 0.518
1.169ArgMet: 1.169 ± 0.37
2.519ArgAsn: 2.519 ± 0.418
1.079ArgPro: 1.079 ± 0.29
1.529ArgGln: 1.529 ± 0.469
1.889ArgArg: 1.889 ± 0.565
1.979ArgSer: 1.979 ± 0.402
2.429ArgThr: 2.429 ± 0.576
2.789ArgVal: 2.789 ± 0.48
0.54ArgTrp: 0.54 ± 0.247
2.519ArgTyr: 2.519 ± 0.714
0.0ArgXaa: 0.0 ± 0.0
Ser
3.598SerAla: 3.598 ± 0.652
0.18SerCys: 0.18 ± 0.146
4.228SerAsp: 4.228 ± 0.827
4.318SerGlu: 4.318 ± 0.828
2.609SerPhe: 2.609 ± 0.678
3.778SerGly: 3.778 ± 0.762
1.349SerHis: 1.349 ± 0.409
5.667SerIle: 5.667 ± 0.571
5.307SerLys: 5.307 ± 1.079
4.228SerLeu: 4.228 ± 0.623
1.439SerMet: 1.439 ± 0.355
4.947SerAsn: 4.947 ± 0.662
0.72SerPro: 0.72 ± 0.264
1.979SerGln: 1.979 ± 0.552
2.249SerArg: 2.249 ± 0.516
3.508SerSer: 3.508 ± 0.679
3.778SerThr: 3.778 ± 0.537
2.519SerVal: 2.519 ± 0.61
0.63SerTrp: 0.63 ± 0.213
2.339SerTyr: 2.339 ± 0.523
0.0SerXaa: 0.0 ± 0.0
Thr
3.238ThrAla: 3.238 ± 0.474
0.36ThrCys: 0.36 ± 0.177
5.037ThrAsp: 5.037 ± 0.951
4.228ThrGlu: 4.228 ± 0.739
1.979ThrPhe: 1.979 ± 0.471
4.318ThrGly: 4.318 ± 0.839
0.989ThrHis: 0.989 ± 0.246
4.318ThrIle: 4.318 ± 0.562
5.487ThrLys: 5.487 ± 0.699
4.138ThrLeu: 4.138 ± 0.546
1.079ThrMet: 1.079 ± 0.293
3.238ThrAsn: 3.238 ± 0.7
1.529ThrPro: 1.529 ± 0.359
1.259ThrGln: 1.259 ± 0.235
2.789ThrArg: 2.789 ± 0.619
4.408ThrSer: 4.408 ± 0.812
3.598ThrThr: 3.598 ± 0.53
3.958ThrVal: 3.958 ± 0.678
0.81ThrTrp: 0.81 ± 0.24
2.968ThrTyr: 2.968 ± 0.632
0.0ThrXaa: 0.0 ± 0.0
Val
2.789ValAla: 2.789 ± 0.485
0.36ValCys: 0.36 ± 0.216
3.238ValAsp: 3.238 ± 0.519
3.868ValGlu: 3.868 ± 0.814
2.069ValPhe: 2.069 ± 0.509
3.958ValGly: 3.958 ± 0.915
0.54ValHis: 0.54 ± 0.223
4.678ValIle: 4.678 ± 0.547
6.207ValLys: 6.207 ± 0.731
4.678ValLeu: 4.678 ± 0.716
1.439ValMet: 1.439 ± 0.479
4.408ValAsn: 4.408 ± 0.453
1.349ValPro: 1.349 ± 0.308
2.069ValGln: 2.069 ± 0.43
1.889ValArg: 1.889 ± 0.518
3.688ValSer: 3.688 ± 0.622
4.947ValThr: 4.947 ± 0.873
4.318ValVal: 4.318 ± 0.773
0.18ValTrp: 0.18 ± 0.112
1.979ValTyr: 1.979 ± 0.41
0.0ValXaa: 0.0 ± 0.0
Trp
0.45TrpAla: 0.45 ± 0.27
0.09TrpCys: 0.09 ± 0.081
0.9TrpAsp: 0.9 ± 0.293
0.54TrpGlu: 0.54 ± 0.155
0.72TrpPhe: 0.72 ± 0.244
0.45TrpGly: 0.45 ± 0.154
0.09TrpHis: 0.09 ± 0.081
0.989TrpIle: 0.989 ± 0.222
0.989TrpLys: 0.989 ± 0.298
0.9TrpLeu: 0.9 ± 0.34
0.72TrpMet: 0.72 ± 0.227
1.079TrpAsn: 1.079 ± 0.402
0.18TrpPro: 0.18 ± 0.113
0.81TrpGln: 0.81 ± 0.283
0.45TrpArg: 0.45 ± 0.175
0.45TrpSer: 0.45 ± 0.198
0.45TrpThr: 0.45 ± 0.151
0.9TrpVal: 0.9 ± 0.301
0.18TrpTrp: 0.18 ± 0.139
0.45TrpTyr: 0.45 ± 0.198
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.159TyrAla: 2.159 ± 0.393
0.36TyrCys: 0.36 ± 0.213
3.148TyrAsp: 3.148 ± 0.478
3.418TyrGlu: 3.418 ± 0.58
2.069TyrPhe: 2.069 ± 0.588
1.979TyrGly: 1.979 ± 0.487
0.72TyrHis: 0.72 ± 0.286
3.418TyrIle: 3.418 ± 0.591
5.577TyrLys: 5.577 ± 0.794
3.238TyrLeu: 3.238 ± 0.688
0.989TyrMet: 0.989 ± 0.284
2.789TyrAsn: 2.789 ± 0.447
0.989TyrPro: 0.989 ± 0.229
2.699TyrGln: 2.699 ± 0.506
1.619TyrArg: 1.619 ± 0.433
2.609TyrSer: 2.609 ± 0.489
1.529TyrThr: 1.529 ± 0.55
2.159TyrVal: 2.159 ± 0.469
0.63TyrTrp: 0.63 ± 0.263
1.529TyrTyr: 1.529 ± 0.498
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 49 proteins (11118 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski