Amino acid dipepetide frequency for Bacillus phage B4

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.113AlaAla: 3.113 ± 0.443
0.471AlaCys: 0.471 ± 0.105
3.666AlaAsp: 3.666 ± 0.34
3.973AlaGlu: 3.973 ± 0.305
2.437AlaPhe: 2.437 ± 0.221
3.563AlaGly: 3.563 ± 0.332
1.126AlaHis: 1.126 ± 0.13
3.83AlaIle: 3.83 ± 0.315
5.161AlaLys: 5.161 ± 0.35
5.407AlaLeu: 5.407 ± 0.363
2.089AlaMet: 2.089 ± 0.209
3.01AlaAsn: 3.01 ± 0.313
2.478AlaPro: 2.478 ± 0.329
2.15AlaGln: 2.15 ± 0.217
2.539AlaArg: 2.539 ± 0.252
3.379AlaSer: 3.379 ± 0.301
3.952AlaThr: 3.952 ± 0.345
3.85AlaVal: 3.85 ± 0.283
0.717AlaTrp: 0.717 ± 0.129
2.724AlaTyr: 2.724 ± 0.235
0.0AlaXaa: 0.0 ± 0.0
Cys
0.41CysAla: 0.41 ± 0.086
0.205CysCys: 0.205 ± 0.066
0.573CysAsp: 0.573 ± 0.112
0.696CysGlu: 0.696 ± 0.118
0.225CysPhe: 0.225 ± 0.058
0.655CysGly: 0.655 ± 0.116
0.143CysHis: 0.143 ± 0.046
0.594CysIle: 0.594 ± 0.127
0.696CysLys: 0.696 ± 0.12
0.553CysLeu: 0.553 ± 0.12
0.225CysMet: 0.225 ± 0.072
0.41CysAsn: 0.41 ± 0.084
0.369CysPro: 0.369 ± 0.082
0.205CysGln: 0.205 ± 0.069
0.348CysArg: 0.348 ± 0.102
0.307CysSer: 0.307 ± 0.08
0.369CysThr: 0.369 ± 0.08
0.532CysVal: 0.532 ± 0.104
0.164CysTrp: 0.164 ± 0.058
0.492CysTyr: 0.492 ± 0.106
0.0CysXaa: 0.0 ± 0.0
Asp
3.645AspAla: 3.645 ± 0.293
0.532AspCys: 0.532 ± 0.095
3.891AspAsp: 3.891 ± 0.292
5.55AspGlu: 5.55 ± 0.373
3.154AspPhe: 3.154 ± 0.3
4.26AspGly: 4.26 ± 0.374
0.655AspHis: 0.655 ± 0.11
5.734AspIle: 5.734 ± 0.338
5.775AspLys: 5.775 ± 0.398
5.181AspLeu: 5.181 ± 0.353
1.986AspMet: 1.986 ± 0.233
3.174AspAsn: 3.174 ± 0.292
1.515AspPro: 1.515 ± 0.205
0.942AspGln: 0.942 ± 0.122
2.744AspArg: 2.744 ± 0.259
2.949AspSer: 2.949 ± 0.226
3.338AspThr: 3.338 ± 0.233
4.362AspVal: 4.362 ± 0.306
0.86AspTrp: 0.86 ± 0.121
3.625AspTyr: 3.625 ± 0.228
0.0AspXaa: 0.0 ± 0.0
Glu
4.915GluAla: 4.915 ± 0.31
0.512GluCys: 0.512 ± 0.106
5.509GluAsp: 5.509 ± 0.387
9.359GluGlu: 9.359 ± 0.834
3.256GluPhe: 3.256 ± 0.223
5.14GluGly: 5.14 ± 0.321
1.597GluHis: 1.597 ± 0.225
5.243GluIle: 5.243 ± 0.393
6.553GluLys: 6.553 ± 0.451
8.806GluLeu: 8.806 ± 0.526
2.519GluMet: 2.519 ± 0.263
4.137GluAsn: 4.137 ± 0.318
1.536GluPro: 1.536 ± 0.173
3.113GluGln: 3.113 ± 0.282
3.42GluArg: 3.42 ± 0.328
3.83GluSer: 3.83 ± 0.324
3.912GluThr: 3.912 ± 0.256
6.267GluVal: 6.267 ± 0.415
1.29GluTrp: 1.29 ± 0.182
3.993GluTyr: 3.993 ± 0.321
0.0GluXaa: 0.0 ± 0.0
Phe
2.253PheAla: 2.253 ± 0.189
0.532PheCys: 0.532 ± 0.099
2.969PheAsp: 2.969 ± 0.268
2.949PheGlu: 2.949 ± 0.263
1.106PhePhe: 1.106 ± 0.163
2.007PheGly: 2.007 ± 0.208
0.819PheHis: 0.819 ± 0.12
2.724PheIle: 2.724 ± 0.22
3.154PheLys: 3.154 ± 0.273
3.154PheLeu: 3.154 ± 0.274
1.085PheMet: 1.085 ± 0.154
2.417PheAsn: 2.417 ± 0.207
1.311PhePro: 1.311 ± 0.165
1.147PheGln: 1.147 ± 0.18
1.454PheArg: 1.454 ± 0.173
2.642PheSer: 2.642 ± 0.216
2.949PheThr: 2.949 ± 0.26
2.785PheVal: 2.785 ± 0.236
0.451PheTrp: 0.451 ± 0.111
1.864PheTyr: 1.864 ± 0.23
0.0PheXaa: 0.0 ± 0.0
Gly
3.686GlyAla: 3.686 ± 0.355
0.614GlyCys: 0.614 ± 0.121
4.096GlyAsp: 4.096 ± 0.314
5.386GlyGlu: 5.386 ± 0.364
2.908GlyPhe: 2.908 ± 0.216
5.816GlyGly: 5.816 ± 0.801
1.331GlyHis: 1.331 ± 0.172
4.424GlyIle: 4.424 ± 0.311
5.386GlyLys: 5.386 ± 0.339
4.669GlyLeu: 4.669 ± 0.336
1.823GlyMet: 1.823 ± 0.201
3.338GlyAsn: 3.338 ± 0.321
0.0GlyPro: 0.0 ± 0.0
2.15GlyGln: 2.15 ± 0.334
2.396GlyArg: 2.396 ± 0.204
3.809GlySer: 3.809 ± 0.312
4.116GlyThr: 4.116 ± 0.486
4.567GlyVal: 4.567 ± 0.318
1.147GlyTrp: 1.147 ± 0.147
3.318GlyTyr: 3.318 ± 0.268
0.0GlyXaa: 0.0 ± 0.0
His
0.963HisAla: 0.963 ± 0.166
0.164HisCys: 0.164 ± 0.054
1.106HisAsp: 1.106 ± 0.143
1.679HisGlu: 1.679 ± 0.209
0.758HisPhe: 0.758 ± 0.131
0.799HisGly: 0.799 ± 0.131
0.389HisHis: 0.389 ± 0.091
1.311HisIle: 1.311 ± 0.174
1.393HisLys: 1.393 ± 0.18
1.597HisLeu: 1.597 ± 0.186
0.41HisMet: 0.41 ± 0.088
1.126HisAsn: 1.126 ± 0.151
0.635HisPro: 0.635 ± 0.119
0.512HisGln: 0.512 ± 0.099
0.717HisArg: 0.717 ± 0.113
0.819HisSer: 0.819 ± 0.166
1.372HisThr: 1.372 ± 0.187
1.556HisVal: 1.556 ± 0.208
0.225HisTrp: 0.225 ± 0.062
0.84HisTyr: 0.84 ± 0.126
0.0HisXaa: 0.0 ± 0.0
Ile
4.546IleAla: 4.546 ± 0.311
0.635IleCys: 0.635 ± 0.117
5.099IleAsp: 5.099 ± 0.347
6.369IleGlu: 6.369 ± 0.395
1.782IlePhe: 1.782 ± 0.194
3.543IleGly: 3.543 ± 0.245
1.106IleHis: 1.106 ± 0.161
4.178IleIle: 4.178 ± 0.328
5.734IleLys: 5.734 ± 0.355
4.526IleLeu: 4.526 ± 0.283
1.556IleMet: 1.556 ± 0.162
4.075IleAsn: 4.075 ± 0.337
2.109IlePro: 2.109 ± 0.194
1.986IleGln: 1.986 ± 0.218
2.744IleArg: 2.744 ± 0.223
3.85IleSer: 3.85 ± 0.242
4.587IleThr: 4.587 ± 0.345
4.055IleVal: 4.055 ± 0.33
0.451IleTrp: 0.451 ± 0.086
2.847IleTyr: 2.847 ± 0.257
0.0IleXaa: 0.0 ± 0.0
Lys
5.386LysAla: 5.386 ± 0.366
0.553LysCys: 0.553 ± 0.106
4.833LysAsp: 4.833 ± 0.319
8.089LysGlu: 8.089 ± 0.576
2.744LysPhe: 2.744 ± 0.204
5.939LysGly: 5.939 ± 0.332
1.393LysHis: 1.393 ± 0.164
4.464LysIle: 4.464 ± 0.296
6.656LysLys: 6.656 ± 0.453
6.471LysLeu: 6.471 ± 0.416
2.539LysMet: 2.539 ± 0.243
4.034LysAsn: 4.034 ± 0.333
2.355LysPro: 2.355 ± 0.286
3.318LysGln: 3.318 ± 0.253
3.686LysArg: 3.686 ± 0.315
4.178LysSer: 4.178 ± 0.35
4.321LysThr: 4.321 ± 0.352
5.898LysVal: 5.898 ± 0.376
1.024LysTrp: 1.024 ± 0.138
3.625LysTyr: 3.625 ± 0.294
0.0LysXaa: 0.0 ± 0.0
Leu
5.058LeuAla: 5.058 ± 0.339
0.655LeuCys: 0.655 ± 0.115
5.652LeuAsp: 5.652 ± 0.365
7.413LeuGlu: 7.413 ± 0.536
3.195LeuPhe: 3.195 ± 0.271
5.304LeuGly: 5.304 ± 0.339
1.556LeuHis: 1.556 ± 0.18
4.813LeuIle: 4.813 ± 0.361
6.226LeuLys: 6.226 ± 0.368
5.734LeuLeu: 5.734 ± 0.453
2.232LeuMet: 2.232 ± 0.219
3.707LeuAsn: 3.707 ± 0.25
2.744LeuPro: 2.744 ± 0.244
2.642LeuGln: 2.642 ± 0.219
4.034LeuArg: 4.034 ± 0.326
4.813LeuSer: 4.813 ± 0.323
5.714LeuThr: 5.714 ± 0.295
5.325LeuVal: 5.325 ± 0.406
0.84LeuTrp: 0.84 ± 0.133
3.359LeuTyr: 3.359 ± 0.273
0.0LeuXaa: 0.0 ± 0.0
Met
1.966MetAla: 1.966 ± 0.193
0.266MetCys: 0.266 ± 0.075
1.556MetAsp: 1.556 ± 0.178
2.253MetGlu: 2.253 ± 0.224
1.085MetPhe: 1.085 ± 0.168
1.597MetGly: 1.597 ± 0.167
0.532MetHis: 0.532 ± 0.096
1.434MetIle: 1.434 ± 0.185
2.806MetLys: 2.806 ± 0.197
2.294MetLeu: 2.294 ± 0.214
0.717MetMet: 0.717 ± 0.123
1.556MetAsn: 1.556 ± 0.18
0.655MetPro: 0.655 ± 0.104
0.963MetGln: 0.963 ± 0.142
1.352MetArg: 1.352 ± 0.158
1.761MetSer: 1.761 ± 0.205
2.15MetThr: 2.15 ± 0.223
1.638MetVal: 1.638 ± 0.165
0.328MetTrp: 0.328 ± 0.081
1.393MetTyr: 1.393 ± 0.163
0.0MetXaa: 0.0 ± 0.0
Asn
3.031AsnAla: 3.031 ± 0.287
0.43AsnCys: 0.43 ± 0.1
3.051AsnAsp: 3.051 ± 0.242
3.42AsnGlu: 3.42 ± 0.269
1.925AsnPhe: 1.925 ± 0.23
4.239AsnGly: 4.239 ± 0.392
1.044AsnHis: 1.044 ± 0.137
3.051AsnIle: 3.051 ± 0.257
4.505AsnLys: 4.505 ± 0.288
4.424AsnLeu: 4.424 ± 0.337
1.986AsnMet: 1.986 ± 0.165
3.215AsnAsn: 3.215 ± 0.294
2.314AsnPro: 2.314 ± 0.284
1.802AsnGln: 1.802 ± 0.203
2.744AsnArg: 2.744 ± 0.221
2.437AsnSer: 2.437 ± 0.293
3.031AsnThr: 3.031 ± 0.305
3.522AsnVal: 3.522 ± 0.233
0.696AsnTrp: 0.696 ± 0.113
2.498AsnTyr: 2.498 ± 0.218
0.0AsnXaa: 0.0 ± 0.0
Pro
1.905ProAla: 1.905 ± 0.277
0.184ProCys: 0.184 ± 0.064
1.761ProAsp: 1.761 ± 0.188
2.58ProGlu: 2.58 ± 0.258
1.536ProPhe: 1.536 ± 0.194
0.86ProGly: 0.86 ± 0.154
0.614ProHis: 0.614 ± 0.126
1.679ProIle: 1.679 ± 0.17
2.089ProLys: 2.089 ± 0.191
2.253ProLeu: 2.253 ± 0.228
0.819ProMet: 0.819 ± 0.137
1.475ProAsn: 1.475 ± 0.19
0.881ProPro: 0.881 ± 0.181
1.147ProGln: 1.147 ± 0.219
1.167ProArg: 1.167 ± 0.14
1.925ProSer: 1.925 ± 0.224
2.068ProThr: 2.068 ± 0.264
2.437ProVal: 2.437 ± 0.25
0.266ProTrp: 0.266 ± 0.071
1.475ProTyr: 1.475 ± 0.156
0.0ProXaa: 0.0 ± 0.0
Gln
2.601GlnAla: 2.601 ± 0.284
0.205GlnCys: 0.205 ± 0.063
1.946GlnAsp: 1.946 ± 0.184
2.58GlnGlu: 2.58 ± 0.241
1.044GlnPhe: 1.044 ± 0.155
2.294GlnGly: 2.294 ± 0.268
0.655GlnHis: 0.655 ± 0.113
2.068GlnIle: 2.068 ± 0.219
2.212GlnLys: 2.212 ± 0.191
2.724GlnLeu: 2.724 ± 0.263
1.024GlnMet: 1.024 ± 0.122
1.761GlnAsn: 1.761 ± 0.195
1.147GlnPro: 1.147 ± 0.181
1.577GlnGln: 1.577 ± 0.259
1.495GlnArg: 1.495 ± 0.181
1.823GlnSer: 1.823 ± 0.179
2.027GlnThr: 2.027 ± 0.232
2.191GlnVal: 2.191 ± 0.206
0.43GlnTrp: 0.43 ± 0.091
1.393GlnTyr: 1.393 ± 0.135
0.0GlnXaa: 0.0 ± 0.0
Arg
2.273ArgAla: 2.273 ± 0.194
0.307ArgCys: 0.307 ± 0.071
2.519ArgAsp: 2.519 ± 0.279
4.034ArgGlu: 4.034 ± 0.321
2.191ArgPhe: 2.191 ± 0.24
3.092ArgGly: 3.092 ± 0.231
0.635ArgHis: 0.635 ± 0.108
3.072ArgIle: 3.072 ± 0.285
3.092ArgLys: 3.092 ± 0.267
4.014ArgLeu: 4.014 ± 0.3
1.413ArgMet: 1.413 ± 0.156
2.13ArgAsn: 2.13 ± 0.23
1.085ArgPro: 1.085 ± 0.163
1.515ArgGln: 1.515 ± 0.161
1.597ArgArg: 1.597 ± 0.179
1.72ArgSer: 1.72 ± 0.164
2.478ArgThr: 2.478 ± 0.246
3.174ArgVal: 3.174 ± 0.298
0.512ArgTrp: 0.512 ± 0.078
2.048ArgTyr: 2.048 ± 0.203
0.0ArgXaa: 0.0 ± 0.0
Ser
3.359SerAla: 3.359 ± 0.301
0.266SerCys: 0.266 ± 0.069
2.929SerAsp: 2.929 ± 0.271
3.359SerGlu: 3.359 ± 0.301
2.867SerPhe: 2.867 ± 0.228
3.686SerGly: 3.686 ± 0.317
1.065SerHis: 1.065 ± 0.17
4.26SerIle: 4.26 ± 0.302
4.464SerLys: 4.464 ± 0.326
4.649SerLeu: 4.649 ± 0.297
1.515SerMet: 1.515 ± 0.166
2.99SerAsn: 2.99 ± 0.256
1.536SerPro: 1.536 ± 0.17
1.311SerGln: 1.311 ± 0.153
2.191SerArg: 2.191 ± 0.224
3.113SerSer: 3.113 ± 0.329
3.318SerThr: 3.318 ± 0.31
3.195SerVal: 3.195 ± 0.262
0.84SerTrp: 0.84 ± 0.137
2.785SerTyr: 2.785 ± 0.238
0.0SerXaa: 0.0 ± 0.0
Thr
3.563ThrAla: 3.563 ± 0.371
0.492ThrCys: 0.492 ± 0.093
3.338ThrAsp: 3.338 ± 0.266
4.342ThrGlu: 4.342 ± 0.327
2.642ThrPhe: 2.642 ± 0.253
4.342ThrGly: 4.342 ± 0.399
0.983ThrHis: 0.983 ± 0.149
4.26ThrIle: 4.26 ± 0.346
4.546ThrLys: 4.546 ± 0.307
5.529ThrLeu: 5.529 ± 0.277
1.393ThrMet: 1.393 ± 0.203
3.686ThrAsn: 3.686 ± 0.408
2.437ThrPro: 2.437 ± 0.306
1.966ThrGln: 1.966 ± 0.232
2.847ThrArg: 2.847 ± 0.238
3.318ThrSer: 3.318 ± 0.305
3.563ThrThr: 3.563 ± 0.269
5.099ThrVal: 5.099 ± 0.434
0.676ThrTrp: 0.676 ± 0.141
3.4ThrTyr: 3.4 ± 0.252
0.0ThrXaa: 0.0 ± 0.0
Val
3.912ValAla: 3.912 ± 0.314
0.492ValCys: 0.492 ± 0.095
4.997ValAsp: 4.997 ± 0.268
6.123ValGlu: 6.123 ± 0.367
2.969ValPhe: 2.969 ± 0.216
4.178ValGly: 4.178 ± 0.273
1.311ValHis: 1.311 ± 0.149
4.014ValIle: 4.014 ± 0.308
5.878ValLys: 5.878 ± 0.391
4.669ValLeu: 4.669 ± 0.324
1.495ValMet: 1.495 ± 0.174
3.543ValAsn: 3.543 ± 0.282
2.478ValPro: 2.478 ± 0.211
2.396ValGln: 2.396 ± 0.22
2.662ValArg: 2.662 ± 0.243
3.707ValSer: 3.707 ± 0.305
5.427ValThr: 5.427 ± 0.434
4.772ValVal: 4.772 ± 0.329
0.635ValTrp: 0.635 ± 0.09
3.318ValTyr: 3.318 ± 0.258
0.0ValXaa: 0.0 ± 0.0
Trp
0.573TrpAla: 0.573 ± 0.122
0.164TrpCys: 0.164 ± 0.048
1.024TrpAsp: 1.024 ± 0.13
0.922TrpGlu: 0.922 ± 0.144
0.43TrpPhe: 0.43 ± 0.092
0.819TrpGly: 0.819 ± 0.129
0.348TrpHis: 0.348 ± 0.077
0.963TrpIle: 0.963 ± 0.156
0.942TrpLys: 0.942 ± 0.125
0.86TrpLeu: 0.86 ± 0.156
0.41TrpMet: 0.41 ± 0.098
0.655TrpAsn: 0.655 ± 0.155
0.0TrpPro: 0.0 ± 0.0
0.389TrpGln: 0.389 ± 0.108
0.512TrpArg: 0.512 ± 0.1
0.778TrpSer: 0.778 ± 0.135
0.717TrpThr: 0.717 ± 0.14
0.819TrpVal: 0.819 ± 0.13
0.184TrpTrp: 0.184 ± 0.056
0.676TrpTyr: 0.676 ± 0.101
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.478TyrAla: 2.478 ± 0.193
0.492TyrCys: 0.492 ± 0.101
3.359TyrAsp: 3.359 ± 0.285
3.83TyrGlu: 3.83 ± 0.282
1.515TyrPhe: 1.515 ± 0.188
2.847TyrGly: 2.847 ± 0.236
1.065TyrHis: 1.065 ± 0.173
3.666TyrIle: 3.666 ± 0.309
4.26TyrLys: 4.26 ± 0.362
3.502TyrLeu: 3.502 ± 0.305
1.044TyrMet: 1.044 ± 0.136
2.929TyrAsn: 2.929 ± 0.226
1.536TyrPro: 1.536 ± 0.162
1.966TyrGln: 1.966 ± 0.242
2.253TyrArg: 2.253 ± 0.217
2.539TyrSer: 2.539 ± 0.177
3.01TyrThr: 3.01 ± 0.24
2.929TyrVal: 2.929 ± 0.227
0.451TyrTrp: 0.451 ± 0.11
2.15TyrTyr: 2.15 ± 0.218
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 277 proteins (48831 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski