Trichechus manatus papillomavirus 1
Average proteome isoelectric point is 6.03
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q5UUY5|Q5UUY5_9PAPI Protein E6 OS=Trichechus manatus papillomavirus 1 OX=291589 GN=E6 PE=3 SV=1
MM1 pKa = 7.43 HH2 pKa = 7.78 GDD4 pKa = 3.23 HH5 pKa = 6.62 ATIPDD10 pKa = 3.83 ILLEE14 pKa = 4.39 ALDD17 pKa = 4.4 LTPHH21 pKa = 5.24 IQEE24 pKa = 4.6 YY25 pKa = 10.6 ADD27 pKa = 3.88 SSAFDD32 pKa = 4.19 LLCHH36 pKa = 6.64 EE37 pKa = 5.05 NLDD40 pKa = 3.95 DD41 pKa = 5.02 PEE43 pKa = 4.26 VEE45 pKa = 4.08 EE46 pKa = 5.0 VKK48 pKa = 10.45 EE49 pKa = 3.95 QEE51 pKa = 4.03 KK52 pKa = 11.42 AEE54 pKa = 4.34 DD55 pKa = 3.56 SGCARR60 pKa = 11.84 TYY62 pKa = 10.09 YY63 pKa = 9.95 QVEE66 pKa = 4.83 SICGGCDD73 pKa = 2.92 KK74 pKa = 10.74 PVAAVVLATHH84 pKa = 6.61 TGIFSLNEE92 pKa = 3.62 NLFTEE97 pKa = 4.64 LFFVCLSCAQRR108 pKa = 11.84 EE109 pKa = 5.08 GYY111 pKa = 10.57 RR112 pKa = 11.84 NDD114 pKa = 3.25 GG115 pKa = 3.55
Molecular weight: 12.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.997
IPC2_protein 4.202
IPC_protein 4.126
Toseland 3.961
ProMoST 4.253
Dawson 4.088
Bjellqvist 4.24
Wikipedia 3.973
Rodwell 3.973
Grimsley 3.872
Solomon 4.088
Lehninger 4.037
Nozaki 4.202
DTASelect 4.368
Thurlkill 3.986
EMBOSS 3.999
Sillero 4.253
Patrickios 0.604
IPC_peptide 4.088
IPC2_peptide 4.228
IPC2.peptide.svr19 4.123
Protein with the highest isoelectric point:
>tr|Q5UUY2|Q5UUY2_9PAPI Regulatory protein E2 OS=Trichechus manatus papillomavirus 1 OX=291589 GN=E2 PE=3 SV=1
MM1 pKa = 7.4 MEE3 pKa = 3.93 TRR5 pKa = 11.84 LCFLPSLLVPLSTSAGTQSQTDD27 pKa = 3.7 PHH29 pKa = 6.78 PLPPEE34 pKa = 4.7 TYY36 pKa = 9.02 PSHH39 pKa = 7.2 PLDD42 pKa = 4.21 PSPVEE47 pKa = 4.4 GPPPTHH53 pKa = 7.21 PSPIPRR59 pKa = 11.84 SPYY62 pKa = 10.01 PQDD65 pKa = 3.25 QHH67 pKa = 6.67 PSLAFGAYY75 pKa = 9.27 HH76 pKa = 6.56 PPIPGEE82 pKa = 3.76 PHH84 pKa = 6.19 RR85 pKa = 11.84 RR86 pKa = 11.84 GYY88 pKa = 7.8 PQRR91 pKa = 11.84 RR92 pKa = 11.84 LRR94 pKa = 11.84 RR95 pKa = 11.84 IRR97 pKa = 11.84 ATRR100 pKa = 11.84 PRR102 pKa = 11.84 EE103 pKa = 3.81 SATRR107 pKa = 11.84 GSWADD112 pKa = 3.26 HH113 pKa = 5.9 ANIRR117 pKa = 11.84 TPTPEE122 pKa = 4.09 EE123 pKa = 3.97 ALQDD127 pKa = 3.62 ALAIILGHH135 pKa = 6.14 WEE137 pKa = 3.99 GDD139 pKa = 3.17 LDD141 pKa = 4.91 LLVTSVRR148 pKa = 11.84 RR149 pKa = 11.84 AVLWYY154 pKa = 9.03 LASVGRR160 pKa = 11.84 TLL162 pKa = 4.74
Molecular weight: 18.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.471
IPC2_protein 7.293
IPC_protein 8.317
Toseland 7.966
ProMoST 7.922
Dawson 8.229
Bjellqvist 8.039
Wikipedia 8.214
Rodwell 8.156
Grimsley 7.79
Solomon 8.697
Lehninger 8.682
Nozaki 8.229
DTASelect 8.287
Thurlkill 8.287
EMBOSS 8.507
Sillero 8.507
Patrickios 4.546
IPC_peptide 8.682
IPC2_peptide 8.17
IPC2.peptide.svr19 8.143
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
2472
115
611
353.1
39.38
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.513 ± 0.389
2.063 ± 0.589
6.068 ± 0.428
5.947 ± 0.614
3.843 ± 0.392
7.16 ± 0.859
2.427 ± 0.509
4.773 ± 0.369
4.167 ± 0.857
9.385 ± 0.854
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.497 ± 0.31
3.479 ± 0.544
7.241 ± 1.175
4.045 ± 0.466
6.108 ± 0.403
7.322 ± 0.799
7.646 ± 0.696
5.218 ± 0.517
1.375 ± 0.316
3.722 ± 0.212
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here