Woodchuck hepatitis virus

Taxonomy: Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes; Blubervirales; Hepadnaviridae; Orthohepadnavirus

Average proteome isoelectric point is 8.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D5L237|D5L237_9HEPA Capsid protein OS=Woodchuck hepatitis virus OX=35269 GN=C PE=3 SV=1
MM1 pKa = 7.82AARR4 pKa = 11.84LCCQLDD10 pKa = 3.52PTRR13 pKa = 11.84DD14 pKa = 3.58VLLLRR19 pKa = 11.84PFSSQSSGPPFPRR32 pKa = 11.84PSAGSAASPASSLSASDD49 pKa = 4.83EE50 pKa = 4.11SDD52 pKa = 3.55LPLGRR57 pKa = 11.84LPACFASASGPCCLVVTCAEE77 pKa = 4.38LRR79 pKa = 11.84TMDD82 pKa = 3.37STVNFVSWHH91 pKa = 6.2ANRR94 pKa = 11.84QLGMPSKK101 pKa = 10.62DD102 pKa = 2.62LWTPYY107 pKa = 10.2IRR109 pKa = 11.84DD110 pKa = 3.37QLLTKK115 pKa = 9.8WEE117 pKa = 4.27EE118 pKa = 3.96GSIDD122 pKa = 3.45PRR124 pKa = 11.84LSIFVLGGCRR134 pKa = 11.84HH135 pKa = 5.53KK136 pKa = 10.98CMRR139 pKa = 11.84LPP141 pKa = 3.79

Molecular weight: 15.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D5L237|D5L237_9HEPA Capsid protein OS=Woodchuck hepatitis virus OX=35269 GN=C PE=3 SV=1
MM1 pKa = 7.38HH2 pKa = 6.83ATAVTMYY9 pKa = 10.4LFHH12 pKa = 7.11LCLVFACVPCPTVQASKK29 pKa = 11.2LCLGWLWGMDD39 pKa = 2.87IDD41 pKa = 4.43PYY43 pKa = 11.43KK44 pKa = 10.76EE45 pKa = 4.12FGSSYY50 pKa = 10.8QLLNFLPLDD59 pKa = 3.88FFPDD63 pKa = 3.91LNALVDD69 pKa = 3.85TATALYY75 pKa = 10.2EE76 pKa = 4.05EE77 pKa = 5.01EE78 pKa = 4.13LTGRR82 pKa = 11.84EE83 pKa = 4.29HH84 pKa = 7.62CSPHH88 pKa = 5.83HH89 pKa = 4.45TTIRR93 pKa = 11.84QALVCWDD100 pKa = 4.27EE101 pKa = 4.07LTKK104 pKa = 11.0LIAWMSSNITSEE116 pKa = 4.01QVRR119 pKa = 11.84TIIVNYY125 pKa = 10.35VNDD128 pKa = 3.36TWGLKK133 pKa = 9.17VRR135 pKa = 11.84QSLWFHH141 pKa = 6.39LSCLTFGQHH150 pKa = 5.07TVQEE154 pKa = 4.43FLVSFGVWIRR164 pKa = 11.84TPAPYY169 pKa = 9.94RR170 pKa = 11.84PPNAPILSTLPEE182 pKa = 3.9HH183 pKa = 5.34TVIRR187 pKa = 11.84RR188 pKa = 11.84RR189 pKa = 11.84GGARR193 pKa = 11.84ASRR196 pKa = 11.84SPRR199 pKa = 11.84RR200 pKa = 11.84RR201 pKa = 11.84TPSPRR206 pKa = 11.84RR207 pKa = 11.84RR208 pKa = 11.84RR209 pKa = 11.84SQSPRR214 pKa = 11.84RR215 pKa = 11.84RR216 pKa = 11.84RR217 pKa = 11.84SQSPSANCC225 pKa = 3.86

Molecular weight: 25.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1671

141

879

417.8

47.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.625 ± 0.907

2.932 ± 0.665

3.232 ± 0.557

2.693 ± 0.395

4.548 ± 0.218

5.266 ± 0.27

3.052 ± 0.791

4.548 ± 0.777

3.411 ± 0.58

12.208 ± 0.333

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.257 ± 0.414

4.608 ± 0.908

8.558 ± 1.172

4.309 ± 0.561

5.984 ± 1.137

8.857 ± 1.515

7.301 ± 0.946

5.625 ± 0.288

3.232 ± 0.586

2.753 ± 0.436
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski