Cotton leaf curl Shahdadpur virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus; unclassified Begomovirus

Average proteome isoelectric point is 7.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D2U4U9|D2U4U9_9GEMI AC4 protein OS=Cotton leaf curl Shahdadpur virus OX=674996 GN=AC4 PE=3 SV=1
MM1 pKa = 7.44PPKK4 pKa = 10.59RR5 pKa = 11.84NGIYY9 pKa = 10.05SKK11 pKa = 10.94NYY13 pKa = 8.55FITYY17 pKa = 7.41PKK19 pKa = 10.48CSLTKK24 pKa = 10.69EE25 pKa = 4.12EE26 pKa = 5.36ALSQLLNIQTPTSKK40 pKa = 10.51KK41 pKa = 9.9YY42 pKa = 10.53IRR44 pKa = 11.84ICRR47 pKa = 11.84GLHH50 pKa = 6.09EE51 pKa = 6.26DD52 pKa = 3.94GTPHH56 pKa = 6.72LHH58 pKa = 6.66VLIQFEE64 pKa = 5.24GKK66 pKa = 10.06FKK68 pKa = 9.6CQNMRR73 pKa = 11.84FFDD76 pKa = 4.05LVSPSRR82 pKa = 11.84SAHH85 pKa = 4.9FHH87 pKa = 6.45PNIQGAKK94 pKa = 9.25SSSDD98 pKa = 3.11VKK100 pKa = 11.24SYY102 pKa = 10.79IDD104 pKa = 3.7KK105 pKa = 11.33DD106 pKa = 3.49GDD108 pKa = 3.67TLEE111 pKa = 4.14WGEE114 pKa = 3.84FQIDD118 pKa = 3.21GRR120 pKa = 11.84SARR123 pKa = 11.84GGQQTANDD131 pKa = 4.05AYY133 pKa = 10.69AAALNAGSKK142 pKa = 10.29SEE144 pKa = 3.84ALRR147 pKa = 11.84VIKK150 pKa = 10.2EE151 pKa = 3.88LAPKK155 pKa = 10.6DD156 pKa = 3.88FVLQSHH162 pKa = 6.43NLNANLDD169 pKa = 4.22RR170 pKa = 11.84IFQEE174 pKa = 3.94PLAPYY179 pKa = 9.82ISPFSSSSFDD189 pKa = 3.41QVPEE193 pKa = 3.95EE194 pKa = 4.04LEE196 pKa = 3.96EE197 pKa = 4.17WVSEE201 pKa = 4.2NVVDD205 pKa = 4.91AAARR209 pKa = 11.84PHH211 pKa = 6.61RR212 pKa = 11.84PQSIVIEE219 pKa = 4.44GDD221 pKa = 3.12SRR223 pKa = 11.84TGKK226 pKa = 8.52TMWARR231 pKa = 11.84SLGPHH236 pKa = 6.71NYY238 pKa = 10.18LCGHH242 pKa = 7.38LDD244 pKa = 4.18LSPKK248 pKa = 10.15VYY250 pKa = 11.07SNDD253 pKa = 2.25AWYY256 pKa = 10.92NVIDD260 pKa = 4.79DD261 pKa = 4.33VDD263 pKa = 3.73PHH265 pKa = 6.08FLKK268 pKa = 10.67HH269 pKa = 5.9FKK271 pKa = 10.69EE272 pKa = 4.43FMGAQRR278 pKa = 11.84DD279 pKa = 3.81WQSNTKK285 pKa = 9.6YY286 pKa = 10.57GKK288 pKa = 9.12PVQIKK293 pKa = 10.41GGIPTIFLCNPGPNSSYY310 pKa = 11.34KK311 pKa = 10.62EE312 pKa = 3.85FLDD315 pKa = 3.97EE316 pKa = 4.83EE317 pKa = 4.8KK318 pKa = 10.25NTALKK323 pKa = 10.38NWAVKK328 pKa = 10.17NAIFITLDD336 pKa = 3.05RR337 pKa = 11.84PLYY340 pKa = 10.76SGTNQSTAQGSEE352 pKa = 3.9EE353 pKa = 3.88AQQEE357 pKa = 4.37EE358 pKa = 4.97EE359 pKa = 4.31SRR361 pKa = 11.84SS362 pKa = 3.6

Molecular weight: 40.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D2U4U6|D2U4U6_9GEMI Replication enhancer OS=Cotton leaf curl Shahdadpur virus OX=674996 GN=AC3 PE=3 SV=1
MM1 pKa = 7.71SKK3 pKa = 10.35RR4 pKa = 11.84PADD7 pKa = 3.87IIISTPASKK16 pKa = 10.3VRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84LNFDD24 pKa = 3.04SPYY27 pKa = 9.79VSRR30 pKa = 11.84AAAPIVRR37 pKa = 11.84VTKK40 pKa = 10.71AKK42 pKa = 10.29AWANRR47 pKa = 11.84PMNRR51 pKa = 11.84KK52 pKa = 7.87PRR54 pKa = 11.84MYY56 pKa = 10.68RR57 pKa = 11.84MYY59 pKa = 10.44RR60 pKa = 11.84SPDD63 pKa = 3.21VPRR66 pKa = 11.84GCEE69 pKa = 4.02GLCKK73 pKa = 10.25VQSFEE78 pKa = 4.13SRR80 pKa = 11.84HH81 pKa = 6.13DD82 pKa = 3.26IQHH85 pKa = 6.35IGKK88 pKa = 8.97VMCVSDD94 pKa = 3.59VTRR97 pKa = 11.84GTGLTHH103 pKa = 7.09RR104 pKa = 11.84VGKK107 pKa = 9.66RR108 pKa = 11.84FCVKK112 pKa = 9.94SVYY115 pKa = 10.52VLGKK119 pKa = 9.41IWMDD123 pKa = 3.35EE124 pKa = 4.04NIKK127 pKa = 9.32TKK129 pKa = 10.6NHH131 pKa = 5.78TNSVMFFLVRR141 pKa = 11.84DD142 pKa = 3.84RR143 pKa = 11.84RR144 pKa = 11.84PVDD147 pKa = 3.13KK148 pKa = 10.28PQDD151 pKa = 3.56FGEE154 pKa = 4.33VFNMFDD160 pKa = 4.29NEE162 pKa = 4.11PSTATVKK169 pKa = 10.38NVHH172 pKa = 6.5RR173 pKa = 11.84DD174 pKa = 3.15RR175 pKa = 11.84YY176 pKa = 8.77QVLRR180 pKa = 11.84KK181 pKa = 9.41WYY183 pKa = 8.08ATVTGGQYY191 pKa = 10.85ASKK194 pKa = 10.24EE195 pKa = 3.73QALVKK200 pKa = 10.65KK201 pKa = 9.58FIRR204 pKa = 11.84VNNYY208 pKa = 7.85VAYY211 pKa = 9.58NQQEE215 pKa = 3.83AGKK218 pKa = 10.08YY219 pKa = 8.01EE220 pKa = 4.07NHH222 pKa = 6.29SEE224 pKa = 4.11NALMLYY230 pKa = 7.58MACTHH235 pKa = 7.06ASNPVYY241 pKa = 9.86ATLKK245 pKa = 9.47IRR247 pKa = 11.84IYY249 pKa = 10.64FYY251 pKa = 11.34DD252 pKa = 3.32SVTNN256 pKa = 3.98

Molecular weight: 29.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1120

100

362

186.7

21.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.536 ± 0.778

2.321 ± 0.527

4.732 ± 0.727

5.089 ± 0.679

4.018 ± 0.483

5.179 ± 0.397

3.75 ± 0.647

5.179 ± 0.868

5.536 ± 0.999

7.143 ± 0.892

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.143 ± 0.577

5.625 ± 0.517

5.625 ± 0.418

5.625 ± 0.72

6.875 ± 1.098

8.929 ± 1.593

5.714 ± 1.071

5.714 ± 1.268

1.339 ± 0.132

3.929 ± 0.547
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski