Chitinophaga alhagiae

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Chitinophagia; Chitinophagales; Chitinophagaceae; Chitinophaga

Average proteome isoelectric point is 7.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2126 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z3MRD7|A0A2Z3MRD7_9BACT Urocanate hydratase OS=Chitinophaga alhagiae OX=2203219 GN=hutU PE=3 SV=1
MM1 pKa = 7.41NVATNNDD8 pKa = 3.83LRR10 pKa = 11.84CTIALASLHH19 pKa = 7.0DD20 pKa = 3.95VLDD23 pKa = 3.92PEE25 pKa = 5.46IGLNVVDD32 pKa = 6.0LGLIYY37 pKa = 10.33QLNFEE42 pKa = 4.25EE43 pKa = 4.77DD44 pKa = 3.22SRR46 pKa = 11.84KK47 pKa = 9.75IFALMTLTTQFCPMGQSIADD67 pKa = 3.55GVKK70 pKa = 10.55NNLSACFPGYY80 pKa = 10.18DD81 pKa = 3.47VEE83 pKa = 5.18LEE85 pKa = 4.33LVFDD89 pKa = 4.71PPWNHH94 pKa = 5.99EE95 pKa = 4.34MISEE99 pKa = 4.42EE100 pKa = 3.97GQNFLNRR107 pKa = 3.79

Molecular weight: 12.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z3MWS1|A0A2Z3MWS1_9BACT Uncharacterized protein OS=Chitinophaga alhagiae OX=2203219 GN=DLD77_06475 PE=4 SV=1
MM1 pKa = 7.9RR2 pKa = 11.84KK3 pKa = 9.02QEE5 pKa = 4.05STNAQNEE12 pKa = 3.82ASILRR17 pKa = 11.84NCGMAYY23 pKa = 8.53TLSVIGGRR31 pKa = 11.84WKK33 pKa = 9.3PTILFSLLNGRR44 pKa = 11.84MRR46 pKa = 11.84YY47 pKa = 9.31SEE49 pKa = 4.34LRR51 pKa = 11.84RR52 pKa = 11.84SISGISEE59 pKa = 3.99RR60 pKa = 11.84MLVAQLRR67 pKa = 11.84EE68 pKa = 4.18LEE70 pKa = 4.08EE71 pKa = 5.89DD72 pKa = 3.72GLVQRR77 pKa = 11.84IIYY80 pKa = 9.6PEE82 pKa = 3.79VPPRR86 pKa = 11.84VEE88 pKa = 4.07YY89 pKa = 10.13TLTPAGVSAEE99 pKa = 4.11PMLQCMSDD107 pKa = 2.38WGNRR111 pKa = 11.84LRR113 pKa = 11.84EE114 pKa = 4.12KK115 pKa = 10.33KK116 pKa = 9.8SRR118 pKa = 11.84RR119 pKa = 11.84VARR122 pKa = 11.84RR123 pKa = 2.96

Molecular weight: 14.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2126

0

2126

798322

18

3194

375.5

41.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.901 ± 0.066

0.809 ± 0.017

4.949 ± 0.034

5.744 ± 0.049

4.573 ± 0.035

7.441 ± 0.051

2.112 ± 0.028

5.991 ± 0.043

5.781 ± 0.05

9.658 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.54 ± 0.025

4.771 ± 0.047

4.4 ± 0.033

4.097 ± 0.03

5.1 ± 0.035

5.539 ± 0.032

5.652 ± 0.057

6.691 ± 0.036

1.317 ± 0.02

3.934 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski