Sphingomonas sp. AAP5

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingomonas; unclassified Sphingomonas

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3972 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4P6XAJ4|A0A4P6XAJ4_9SPHN GAF domain-containing protein OS=Sphingomonas sp. AAP5 OX=1523415 GN=E2E30_01610 PE=4 SV=1
MM1 pKa = 7.95WYY3 pKa = 7.87FTWVLGLGLAVGFGILNGMWHH24 pKa = 6.64EE25 pKa = 4.13FHH27 pKa = 7.13LPADD31 pKa = 4.83DD32 pKa = 4.74EE33 pKa = 4.66DD34 pKa = 4.63APPPAA39 pKa = 5.73

Molecular weight: 4.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4P6X758|A0A4P6X758_9SPHN HNOB domain-containing protein OS=Sphingomonas sp. AAP5 OX=1523415 GN=E2E30_03155 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.58GFRR19 pKa = 11.84SRR21 pKa = 11.84MATPGGRR28 pKa = 11.84NVIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84NRR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.0KK41 pKa = 10.58LSAA44 pKa = 4.03

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3972

0

3972

1285990

29

1934

323.8

34.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.952 ± 0.061

0.741 ± 0.01

5.921 ± 0.026

4.855 ± 0.038

3.485 ± 0.024

8.884 ± 0.034

1.989 ± 0.02

4.992 ± 0.023

2.882 ± 0.029

9.921 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.266 ± 0.02

2.412 ± 0.028

5.389 ± 0.024

3.019 ± 0.02

7.237 ± 0.035

5.176 ± 0.029

5.777 ± 0.027

7.454 ± 0.027

1.411 ± 0.016

2.237 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski