Capybara microvirus Cap3_SP_446
Average proteome isoelectric point is 6.17
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W8M5|A0A4P8W8M5_9VIRU Uncharacterized protein OS=Capybara microvirus Cap3_SP_446 OX=2585458 PE=4 SV=1
MM1 pKa = 7.32 VNNLYY6 pKa = 9.95 TIYY9 pKa = 9.49 DD10 pKa = 4.1 TLSQRR15 pKa = 11.84 YY16 pKa = 8.23 GSVVAFASDD25 pKa = 2.99 GMALFSIAQSYY36 pKa = 7.76 QASGEE41 pKa = 4.04 VLDD44 pKa = 5.31 RR45 pKa = 11.84 YY46 pKa = 8.29 EE47 pKa = 4.14 LCRR50 pKa = 11.84 VGTVDD55 pKa = 3.13 IEE57 pKa = 4.51 TGVVEE62 pKa = 4.43 SSGAPVRR69 pKa = 11.84 LAWSSRR75 pKa = 11.84 PQFEE79 pKa = 4.43 QKK81 pKa = 8.11 MTDD84 pKa = 3.24 SVKK87 pKa = 10.96 DD88 pKa = 3.82 PLPVPPLPKK97 pKa = 10.06 QQ98 pKa = 3.19
Molecular weight: 10.78 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.659
IPC2_protein 4.8
IPC_protein 4.584
Toseland 4.418
ProMoST 4.698
Dawson 4.533
Bjellqvist 4.685
Wikipedia 4.431
Rodwell 4.418
Grimsley 4.329
Solomon 4.533
Lehninger 4.482
Nozaki 4.66
DTASelect 4.825
Thurlkill 4.431
EMBOSS 4.444
Sillero 4.698
Patrickios 3.719
IPC_peptide 4.533
IPC2_peptide 4.673
IPC2.peptide.svr19 4.714
Protein with the highest isoelectric point:
>tr|A0A4P8W8M5|A0A4P8W8M5_9VIRU Uncharacterized protein OS=Capybara microvirus Cap3_SP_446 OX=2585458 PE=4 SV=1
MM1 pKa = 7.2 NVVAMTSAKK10 pKa = 9.85 INKK13 pKa = 8.05 PRR15 pKa = 11.84 KK16 pKa = 7.93 LPKK19 pKa = 9.37 WLEE22 pKa = 4.06 LEE24 pKa = 5.27 DD25 pKa = 3.96 IPEE28 pKa = 3.9 TRR30 pKa = 11.84 YY31 pKa = 9.87 PYY33 pKa = 11.16 ALYY36 pKa = 10.55 NFRR39 pKa = 11.84 FGAYY43 pKa = 9.81 ALRR46 pKa = 11.84 VEE48 pKa = 4.56 YY49 pKa = 10.3 NYY51 pKa = 10.52 RR52 pKa = 11.84 HH53 pKa = 5.95 HH54 pKa = 6.49 QFEE57 pKa = 4.98 LIEE60 pKa = 4.51 NGVSKK65 pKa = 10.17 PHH67 pKa = 7.03 LDD69 pKa = 3.46 VLVLFSRR76 pKa = 11.84 DD77 pKa = 3.06 FKK79 pKa = 11.05 YY80 pKa = 10.74 IRR82 pKa = 11.84 DD83 pKa = 3.67 KK84 pKa = 11.05 FLEE87 pKa = 4.33 YY88 pKa = 10.48 VDD90 pKa = 4.09 KK91 pKa = 10.83 FYY93 pKa = 10.98 FAFRR97 pKa = 11.84 CGCDD101 pKa = 3.14 PADD104 pKa = 4.14 LRR106 pKa = 11.84 GG107 pKa = 3.32
Molecular weight: 12.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.165
IPC2_protein 8.17
IPC_protein 8.287
Toseland 8.478
ProMoST 8.521
Dawson 8.843
Bjellqvist 8.873
Wikipedia 8.931
Rodwell 8.873
Grimsley 8.492
Solomon 9.077
Lehninger 9.033
Nozaki 8.873
DTASelect 8.741
Thurlkill 8.77
EMBOSS 8.99
Sillero 9.004
Patrickios 4.139
IPC_peptide 9.063
IPC2_peptide 7.775
IPC2.peptide.svr19 7.768
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1612
98
586
268.7
30.19
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.491 ± 1.72
1.427 ± 0.735
7.382 ± 0.393
5.583 ± 0.814
4.777 ± 0.675
6.079 ± 0.534
1.303 ± 0.325
4.901 ± 0.314
6.266 ± 1.126
7.878 ± 1.019
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.419 ± 0.16
4.342 ± 0.264
4.032 ± 0.69
3.722 ± 0.609
5.583 ± 0.506
8.561 ± 0.772
4.777 ± 0.415
4.901 ± 0.811
0.868 ± 0.202
5.707 ± 0.441
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here