Dactylellina haptotyla (strain CBS 200.50) (Nematode-trapping fungus) (Monacrosporium haptotylum)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; Orbiliomycetes; Orbiliales; Orbiliaceae; Dactylellina; Dactylellina haptotyla

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10959 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S7ZZP1|S7ZZP1_DACHA Uncharacterized protein OS=Dactylellina haptotyla (strain CBS 200.50) OX=1284197 GN=H072_10625 PE=4 SV=1
MM1 pKa = 7.08YY2 pKa = 10.56VHH4 pKa = 7.02LSVILVAALISTGNSLALPRR24 pKa = 11.84PDD26 pKa = 3.67NVRR29 pKa = 11.84PPCTKK34 pKa = 10.52YY35 pKa = 9.63IYY37 pKa = 10.29QWVFPNWGRR46 pKa = 11.84TEE48 pKa = 4.15TVHH51 pKa = 6.78AATVTSTYY59 pKa = 7.07TTNCGRR65 pKa = 11.84CQDD68 pKa = 3.51IAVTTMEE75 pKa = 4.46IALARR80 pKa = 11.84QWEE83 pKa = 4.25MLRR86 pKa = 11.84YY87 pKa = 8.11TSNLQKK93 pKa = 8.26TTTITLPITKK103 pKa = 10.0AEE105 pKa = 3.85WVYY108 pKa = 10.16TCSPTSSGDD117 pKa = 3.59LGPEE121 pKa = 3.8ATGTAAEE128 pKa = 4.62TPSGDD133 pKa = 3.98SGSGAAPMFFGGGGGATNSSSGGSSGDD160 pKa = 3.28GTGGVGGEE168 pKa = 4.13GGMGVGGAQGGSGMGGEE185 pKa = 4.5GGGNVVSSSTLGGAAGGDD203 pKa = 3.76PGNGGGGSGGGGDD216 pKa = 3.4MGGGGGGGGGGGEE229 pKa = 4.13MEE231 pKa = 5.05AGAVLPNPSVLVLWSDD247 pKa = 3.67TSSITSTPTDD257 pKa = 3.25STTATDD263 pKa = 3.59ASSMSLYY270 pKa = 10.8SGATSTSADD279 pKa = 3.5SEE281 pKa = 4.42ATSVGLDD288 pKa = 3.33STTTTQEE295 pKa = 4.13SPQVTDD301 pKa = 3.35TPITLEE307 pKa = 3.79ATGLVEE313 pKa = 4.79NSNTEE318 pKa = 3.72SGTTGGGDD326 pKa = 3.28SSSSSGGDD334 pKa = 3.14SSSGSGSDD342 pKa = 3.36NGSDD346 pKa = 4.0SGNSDD351 pKa = 3.03GGGDD355 pKa = 3.46GGTT358 pKa = 3.69

Molecular weight: 34.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S8A749|S8A749_DACHA Uncharacterized protein OS=Dactylellina haptotyla (strain CBS 200.50) OX=1284197 GN=H072_7433 PE=4 SV=1
MM1 pKa = 8.04PYY3 pKa = 10.32RR4 pKa = 11.84RR5 pKa = 11.84THH7 pKa = 5.85RR8 pKa = 11.84RR9 pKa = 11.84TRR11 pKa = 11.84PATVRR16 pKa = 11.84TTRR19 pKa = 11.84TKK21 pKa = 10.24PSLMDD26 pKa = 3.54RR27 pKa = 11.84LTGRR31 pKa = 11.84RR32 pKa = 11.84SHH34 pKa = 5.47TTVTTTRR41 pKa = 11.84ATHH44 pKa = 4.89GHH46 pKa = 5.23GHH48 pKa = 5.86HH49 pKa = 6.72HH50 pKa = 6.27HH51 pKa = 6.79HH52 pKa = 6.52VAPVAPVHH60 pKa = 5.17HH61 pKa = 6.48QKK63 pKa = 10.64RR64 pKa = 11.84KK65 pKa = 7.44PTLSDD70 pKa = 3.28KK71 pKa = 11.22VSGALMKK78 pKa = 10.54IKK80 pKa = 10.51GSLTRR85 pKa = 11.84RR86 pKa = 11.84PGLKK90 pKa = 10.05AAGTRR95 pKa = 11.84RR96 pKa = 11.84QRR98 pKa = 11.84GTDD101 pKa = 2.82GRR103 pKa = 11.84GSHH106 pKa = 5.81RR107 pKa = 11.84RR108 pKa = 11.84RR109 pKa = 11.84WW110 pKa = 3.3

Molecular weight: 12.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10959

0

10959

5669352

22

11200

517.3

57.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.858 ± 0.021

1.193 ± 0.009

5.697 ± 0.016

6.36 ± 0.029

3.729 ± 0.016

6.767 ± 0.024

2.137 ± 0.012

5.405 ± 0.019

5.477 ± 0.023

8.446 ± 0.026

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.091 ± 0.008

4.07 ± 0.015

6.128 ± 0.027

3.808 ± 0.02

5.599 ± 0.022

8.337 ± 0.029

6.63 ± 0.046

5.95 ± 0.019

1.358 ± 0.008

2.96 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski