Wencheng Sm shrew coronavirus
Average proteome isoelectric point is 7.3
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A240FW19|A0A240FW19_9ALPC Membrane protein OS=Wencheng Sm shrew coronavirus OX=1508228 GN=M PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 10.58 LIILFLVLSCVEE14 pKa = 3.78 CVEE17 pKa = 4.65 YY18 pKa = 10.4 IKK20 pKa = 10.96 CSNTQLGLLDD30 pKa = 4.44 LEE32 pKa = 5.07 KK33 pKa = 9.85 ITYY36 pKa = 10.48 SLFDD40 pKa = 4.3 LGDD43 pKa = 3.4 VGVNSTTLINGYY55 pKa = 9.57 FPSKK59 pKa = 10.65 DD60 pKa = 3.17 INNWLCEE67 pKa = 3.91 KK68 pKa = 10.15 MGVSEE73 pKa = 5.16 LYY75 pKa = 10.43 YY76 pKa = 10.77 GQAFYY81 pKa = 10.44 TILNNVGSASLKK93 pKa = 10.57 FLIQPIVYY101 pKa = 9.7 SNSSYY106 pKa = 10.78 VIYY109 pKa = 9.36 MAYY112 pKa = 9.78 KK113 pKa = 9.98 SGVLNFSICYY123 pKa = 9.39 RR124 pKa = 11.84 NYY126 pKa = 10.39 FSFSWDD132 pKa = 3.45 TDD134 pKa = 3.59 PPSPISCAFNRR145 pKa = 11.84 RR146 pKa = 11.84 IYY148 pKa = 10.38 FGHH151 pKa = 6.8 RR152 pKa = 11.84 YY153 pKa = 9.3 IYY155 pKa = 10.55 LGVIWRR161 pKa = 11.84 HH162 pKa = 5.38 GYY164 pKa = 10.08 VKK166 pKa = 10.35 IITPNFVQQFEE177 pKa = 4.74 LDD179 pKa = 3.45 NMYY182 pKa = 11.05 LEE184 pKa = 4.98 VIRR187 pKa = 11.84 VATGRR192 pKa = 11.84 FGSTVNYY199 pKa = 9.04 VIVMPFQIVDD209 pKa = 3.24 KK210 pKa = 10.87 RR211 pKa = 11.84 STFLVNYY218 pKa = 7.53 NQSGYY223 pKa = 10.78 VSNLLNCSEE232 pKa = 4.78 SIEE235 pKa = 4.14 AQLKK239 pKa = 8.85 CKK241 pKa = 10.28 SRR243 pKa = 11.84 SFEE246 pKa = 4.18 LQSGVYY252 pKa = 9.75 EE253 pKa = 3.96 IALEE257 pKa = 4.02 EE258 pKa = 4.13 RR259 pKa = 11.84 VGGYY263 pKa = 10.17 YY264 pKa = 10.4 SVVTNVTGDD273 pKa = 3.76 CNNIKK278 pKa = 10.42 HH279 pKa = 6.2 LFSNFTGNRR288 pKa = 11.84 QPLYY292 pKa = 10.07 AGNFASMSCIVPFNFSINNDD312 pKa = 3.64 LLNNNPQLRR321 pKa = 11.84 GFNGEE326 pKa = 4.17 FKK328 pKa = 10.85 PFIDD332 pKa = 4.44 CKK334 pKa = 10.59 GINPIGLFGSNGTCYY349 pKa = 10.7 SGMEE353 pKa = 3.51 WRR355 pKa = 11.84 YY356 pKa = 3.85 WTRR359 pKa = 11.84 RR360 pKa = 11.84 FSGYY364 pKa = 10.89 NMSIEE369 pKa = 4.71 GIAEE373 pKa = 3.96 SFYY376 pKa = 11.27 DD377 pKa = 3.74 AGIGEE382 pKa = 4.27 NEE384 pKa = 4.65 CYY386 pKa = 10.67 GLLKK390 pKa = 10.29 GRR392 pKa = 11.84 QYY394 pKa = 11.66 NEE396 pKa = 3.45 FWQFTEE402 pKa = 3.61 VLYY405 pKa = 10.7 YY406 pKa = 10.26 KK407 pKa = 10.54 VRR409 pKa = 11.84 FMEE412 pKa = 4.93 KK413 pKa = 10.46 GLDD416 pKa = 3.41 VCYY419 pKa = 10.95 GNLPPAPQVNKK430 pKa = 10.32 CNVWTFNDD438 pKa = 3.13 ITFEE442 pKa = 4.18 GVLLEE447 pKa = 4.28 TNKK450 pKa = 9.29 TFNPQLKK457 pKa = 9.64 ALYY460 pKa = 8.51 KK461 pKa = 10.09 GSMVVMIRR469 pKa = 11.84 VFGIVYY475 pKa = 9.41 EE476 pKa = 4.03 VVPCIKK482 pKa = 9.7 TRR484 pKa = 11.84 LSVIYY489 pKa = 10.14 NANNKK494 pKa = 9.68 NYY496 pKa = 7.97 STLYY500 pKa = 10.25 RR501 pKa = 11.84 VANCNQIDD509 pKa = 4.24 TKK511 pKa = 10.96 RR512 pKa = 11.84 SVHH515 pKa = 5.6 PVGLLSRR522 pKa = 11.84 DD523 pKa = 4.29 GINTPCGCLRR533 pKa = 11.84 NAMFSPNITVDD544 pKa = 3.33 SYY546 pKa = 11.71 SCKK549 pKa = 9.76 TLLSDD554 pKa = 4.54 GYY556 pKa = 11.17 CVVFNNGSTEE566 pKa = 3.98 VVPIGIVGANTTYY579 pKa = 11.35 NMPILEE585 pKa = 4.15 EE586 pKa = 4.35 SFVEE590 pKa = 4.08 LALDD594 pKa = 3.36 HH595 pKa = 6.45 VLVSEE600 pKa = 4.37 SEE602 pKa = 4.35 YY603 pKa = 10.71 FQTEE607 pKa = 4.13 MPVFDD612 pKa = 5.1 IDD614 pKa = 3.84 CEE616 pKa = 4.62 KK617 pKa = 10.92 YY618 pKa = 10.04 ICDD621 pKa = 3.51 NSEE624 pKa = 3.87 SCRR627 pKa = 11.84 MLLEE631 pKa = 4.19 QYY633 pKa = 11.17 AGFCSKK639 pKa = 9.12 ITSDD643 pKa = 3.94 VKK645 pKa = 11.31 GNSQLLNANTLAMYY659 pKa = 10.66 KK660 pKa = 10.4 NIVPSDD666 pKa = 3.84 SIKK669 pKa = 10.51 PIEE672 pKa = 4.31 NFGDD676 pKa = 3.92 FNMSMFIDD684 pKa = 3.44 KK685 pKa = 10.48 SGRR688 pKa = 11.84 SVLEE692 pKa = 4.02 DD693 pKa = 3.53 LLFDD697 pKa = 5.27 KK698 pKa = 10.72 IVTTGPGFYY707 pKa = 10.02 EE708 pKa = 4.98 DD709 pKa = 4.46 YY710 pKa = 10.12 YY711 pKa = 11.16 KK712 pKa = 11.06 CRR714 pKa = 11.84 TTTVKK719 pKa = 10.8 DD720 pKa = 3.65 LVCAQYY726 pKa = 11.39 YY727 pKa = 8.4 NGIMIIPPIQDD738 pKa = 3.12 AEE740 pKa = 4.49 TIGMYY745 pKa = 10.51 GGIVAAGMTMGLFGGQATMGTWQIAMSARR774 pKa = 11.84 LNAVGVTQGMIMEE787 pKa = 5.23 DD788 pKa = 3.64 VQKK791 pKa = 10.72 LANGFNTLATSVSKK805 pKa = 9.93 MASVTADD812 pKa = 3.17 ALSRR816 pKa = 11.84 IQGVVNANALQVEE829 pKa = 4.62 LLARR833 pKa = 11.84 ATMDD837 pKa = 3.08 NFGAISSNFEE847 pKa = 4.07 IIEE850 pKa = 4.04 KK851 pKa = 10.31 RR852 pKa = 11.84 LKK854 pKa = 9.94 QVQAEE859 pKa = 4.09 QQMDD863 pKa = 3.53 RR864 pKa = 11.84 VINGKK869 pKa = 8.63 MNALLNFVSNYY880 pKa = 8.58 KK881 pKa = 10.57 LKK883 pKa = 10.72 VSEE886 pKa = 4.3 LTSIQQLVKK895 pKa = 10.8 QKK897 pKa = 10.19 VQEE900 pKa = 4.32 CVHH903 pKa = 5.87 AQSLRR908 pKa = 11.84 NGFCGSGLHH917 pKa = 6.54 VMSIPQLVPNGVMFLHH933 pKa = 7.11 FSLVKK938 pKa = 10.26 NNSIIVKK945 pKa = 7.12 QTPGLCKK952 pKa = 9.73 EE953 pKa = 4.58 DD954 pKa = 4.44 KK955 pKa = 10.48 INCIVPKK962 pKa = 10.57 SGIFVSKK969 pKa = 10.77 NDD971 pKa = 3.83 SNWYY975 pKa = 5.94 ITPRR979 pKa = 11.84 NKK981 pKa = 9.99 YY982 pKa = 8.62 VPKK985 pKa = 10.6 NITNSDD991 pKa = 4.04 IIFTTYY997 pKa = 10.73 SEE999 pKa = 4.36 NFTVINNTIQFPGIEE1014 pKa = 4.38 FPQDD1018 pKa = 2.77 GDD1020 pKa = 3.41 FDD1022 pKa = 4.2 GQIKK1026 pKa = 10.54 NVTLEE1031 pKa = 4.07 LQSLKK1036 pKa = 11.09 DD1037 pKa = 3.43 VVANMSYY1044 pKa = 11.41 LDD1046 pKa = 4.25 LSNHH1050 pKa = 5.2 TALLKK1055 pKa = 10.88 NISEE1059 pKa = 4.58 EE1060 pKa = 4.19 VKK1062 pKa = 10.74 KK1063 pKa = 10.82 MNITVSQFKK1072 pKa = 10.68 QYY1074 pKa = 10.53 VKK1076 pKa = 9.85 YY1077 pKa = 10.88 VKK1079 pKa = 9.66 WPWYY1083 pKa = 8.28 VWLLIVMALIAFGLVCFWIFMCTGCCGLCDD1113 pKa = 4.87 CIRR1116 pKa = 11.84 RR1117 pKa = 11.84 SCCDD1121 pKa = 3.14 SNTYY1125 pKa = 10.54 EE1126 pKa = 4.81 PIEE1129 pKa = 4.81 KK1130 pKa = 9.87 IHH1132 pKa = 5.99 IQQ1134 pKa = 3.28
Molecular weight: 127.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.523
IPC2_protein 5.97
IPC_protein 5.97
Toseland 5.957
ProMoST 6.313
Dawson 6.249
Bjellqvist 6.3
Wikipedia 6.237
Rodwell 6.237
Grimsley 5.982
Solomon 6.249
Lehninger 6.249
Nozaki 6.532
DTASelect 6.664
Thurlkill 6.664
EMBOSS 6.62
Sillero 6.634
Patrickios 1.519
IPC_peptide 6.275
IPC2_peptide 6.491
IPC2.peptide.svr19 6.498
Protein with the highest isoelectric point:
>tr|A0A240FW20|A0A240FW20_9ALPC Nucleocapsid protein OS=Wencheng Sm shrew coronavirus OX=1508228 PE=4 SV=1
MM1 pKa = 7.21 SAEE4 pKa = 4.18 KK5 pKa = 10.79 VSWSSSLPTKK15 pKa = 10.76 ADD17 pKa = 3.29 TKK19 pKa = 10.6 NGSKK23 pKa = 10.57 VEE25 pKa = 4.19 VEE27 pKa = 4.21 LSAWDD32 pKa = 4.38 PIIIQNNKK40 pKa = 9.49 NIQDD44 pKa = 3.75 VLPRR48 pKa = 11.84 TSVPKK53 pKa = 10.36 GLPSGHH59 pKa = 6.89 EE60 pKa = 3.01 IGYY63 pKa = 8.73 WYY65 pKa = 10.25 KK66 pKa = 10.57 GPEE69 pKa = 3.71 RR70 pKa = 11.84 YY71 pKa = 9.73 RR72 pKa = 11.84 FTRR75 pKa = 11.84 GEE77 pKa = 4.25 KK78 pKa = 10.68 VPLDD82 pKa = 4.07 PKK84 pKa = 10.2 WYY86 pKa = 9.62 FYY88 pKa = 10.77 PLGHH92 pKa = 6.72 GPAANKK98 pKa = 9.38 KK99 pKa = 8.03 WNSKK103 pKa = 9.7 VEE105 pKa = 4.15 GVFWVARR112 pKa = 11.84 DD113 pKa = 3.43 GASIEE118 pKa = 4.15 KK119 pKa = 10.67 VPDD122 pKa = 3.65 FEE124 pKa = 4.43 PRR126 pKa = 11.84 KK127 pKa = 10.13 KK128 pKa = 10.22 GAEE131 pKa = 4.02 AKK133 pKa = 9.89 RR134 pKa = 11.84 VRR136 pKa = 11.84 LPGKK140 pKa = 10.31 APANVYY146 pKa = 7.6 FTTTSASRR154 pKa = 11.84 SQSRR158 pKa = 11.84 DD159 pKa = 2.72 NSRR162 pKa = 11.84 AASRR166 pKa = 11.84 SGSKK170 pKa = 10.33 SRR172 pKa = 11.84 IQSRR176 pKa = 11.84 SPSAEE181 pKa = 3.68 RR182 pKa = 11.84 GHH184 pKa = 8.04 VDD186 pKa = 2.46 IKK188 pKa = 11.11 LAVLEE193 pKa = 4.05 ALKK196 pKa = 10.87 DD197 pKa = 3.66 LGIGQDD203 pKa = 3.53 TKK205 pKa = 11.27 KK206 pKa = 10.55 KK207 pKa = 9.22 SSKK210 pKa = 10.21 SAAASGNNTPKK221 pKa = 10.22 QEE223 pKa = 4.34 SSPKK227 pKa = 8.63 VQRR230 pKa = 11.84 KK231 pKa = 7.91 QIEE234 pKa = 4.19 RR235 pKa = 11.84 LRR237 pKa = 11.84 HH238 pKa = 4.91 KK239 pKa = 10.31 RR240 pKa = 11.84 VPTGKK245 pKa = 10.41 EE246 pKa = 3.68 NITVCFGPRR255 pKa = 11.84 GPEE258 pKa = 3.58 QNFGSDD264 pKa = 3.54 EE265 pKa = 4.12 IVNGGAQGKK274 pKa = 9.16 KK275 pKa = 8.73 VAQLLEE281 pKa = 4.29 NVPGPSALLFGGKK294 pKa = 9.2 VEE296 pKa = 5.11 QIGKK300 pKa = 10.29 ANGKK304 pKa = 9.24 VAVQYY309 pKa = 10.42 TYY311 pKa = 11.2 VAEE314 pKa = 4.57 LDD316 pKa = 3.84 DD317 pKa = 4.2 NEE319 pKa = 4.27 EE320 pKa = 4.01 VKK322 pKa = 10.82 RR323 pKa = 11.84 FLDD326 pKa = 5.62 LIDD329 pKa = 4.85 AYY331 pKa = 10.01 KK332 pKa = 10.69 KK333 pKa = 8.49 PTTANSINSVLNPQATAFAPPSGVIEE359 pKa = 4.17 EE360 pKa = 4.77 LQDD363 pKa = 4.05 DD364 pKa = 4.5 LNN366 pKa = 4.44
Molecular weight: 40.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.911
IPC2_protein 9.019
IPC_protein 8.916
Toseland 9.94
ProMoST 9.502
Dawson 10.087
Bjellqvist 9.692
Wikipedia 10.204
Rodwell 10.687
Grimsley 10.131
Solomon 10.101
Lehninger 10.087
Nozaki 9.911
DTASelect 9.677
Thurlkill 9.955
EMBOSS 10.321
Sillero 9.999
Patrickios 10.335
IPC_peptide 10.116
IPC2_peptide 8.039
IPC2.peptide.svr19 7.998
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
8128
75
6324
1625.6
183.71
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.798 ± 0.75
3.088 ± 0.665
5.376 ± 0.869
4.86 ± 0.415
6.115 ± 0.731
6.299 ± 0.424
1.513 ± 0.255
5.598 ± 0.831
7.16 ± 0.891
8.981 ± 0.715
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.387 ± 0.49
7.013 ± 0.574
2.879 ± 1.123
2.842 ± 0.479
3.027 ± 0.67
7.013 ± 0.665
4.737 ± 0.325
10.162 ± 0.954
1.28 ± 0.276
4.872 ± 0.488
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here