Wencheng Sm shrew coronavirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae; Alphacoronavirus; unclassified Alphacoronavirus

Average proteome isoelectric point is 7.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A240FW19|A0A240FW19_9ALPC Membrane protein OS=Wencheng Sm shrew coronavirus OX=1508228 GN=M PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 10.58LIILFLVLSCVEE14 pKa = 3.78CVEE17 pKa = 4.65YY18 pKa = 10.4IKK20 pKa = 10.96CSNTQLGLLDD30 pKa = 4.44LEE32 pKa = 5.07KK33 pKa = 9.85ITYY36 pKa = 10.48SLFDD40 pKa = 4.3LGDD43 pKa = 3.4VGVNSTTLINGYY55 pKa = 9.57FPSKK59 pKa = 10.65DD60 pKa = 3.17INNWLCEE67 pKa = 3.91KK68 pKa = 10.15MGVSEE73 pKa = 5.16LYY75 pKa = 10.43YY76 pKa = 10.77GQAFYY81 pKa = 10.44TILNNVGSASLKK93 pKa = 10.57FLIQPIVYY101 pKa = 9.7SNSSYY106 pKa = 10.78VIYY109 pKa = 9.36MAYY112 pKa = 9.78KK113 pKa = 9.98SGVLNFSICYY123 pKa = 9.39RR124 pKa = 11.84NYY126 pKa = 10.39FSFSWDD132 pKa = 3.45TDD134 pKa = 3.59PPSPISCAFNRR145 pKa = 11.84RR146 pKa = 11.84IYY148 pKa = 10.38FGHH151 pKa = 6.8RR152 pKa = 11.84YY153 pKa = 9.3IYY155 pKa = 10.55LGVIWRR161 pKa = 11.84HH162 pKa = 5.38GYY164 pKa = 10.08VKK166 pKa = 10.35IITPNFVQQFEE177 pKa = 4.74LDD179 pKa = 3.45NMYY182 pKa = 11.05LEE184 pKa = 4.98VIRR187 pKa = 11.84VATGRR192 pKa = 11.84FGSTVNYY199 pKa = 9.04VIVMPFQIVDD209 pKa = 3.24KK210 pKa = 10.87RR211 pKa = 11.84STFLVNYY218 pKa = 7.53NQSGYY223 pKa = 10.78VSNLLNCSEE232 pKa = 4.78SIEE235 pKa = 4.14AQLKK239 pKa = 8.85CKK241 pKa = 10.28SRR243 pKa = 11.84SFEE246 pKa = 4.18LQSGVYY252 pKa = 9.75EE253 pKa = 3.96IALEE257 pKa = 4.02EE258 pKa = 4.13RR259 pKa = 11.84VGGYY263 pKa = 10.17YY264 pKa = 10.4SVVTNVTGDD273 pKa = 3.76CNNIKK278 pKa = 10.42HH279 pKa = 6.2LFSNFTGNRR288 pKa = 11.84QPLYY292 pKa = 10.07AGNFASMSCIVPFNFSINNDD312 pKa = 3.64LLNNNPQLRR321 pKa = 11.84GFNGEE326 pKa = 4.17FKK328 pKa = 10.85PFIDD332 pKa = 4.44CKK334 pKa = 10.59GINPIGLFGSNGTCYY349 pKa = 10.7SGMEE353 pKa = 3.51WRR355 pKa = 11.84YY356 pKa = 3.85WTRR359 pKa = 11.84RR360 pKa = 11.84FSGYY364 pKa = 10.89NMSIEE369 pKa = 4.71GIAEE373 pKa = 3.96SFYY376 pKa = 11.27DD377 pKa = 3.74AGIGEE382 pKa = 4.27NEE384 pKa = 4.65CYY386 pKa = 10.67GLLKK390 pKa = 10.29GRR392 pKa = 11.84QYY394 pKa = 11.66NEE396 pKa = 3.45FWQFTEE402 pKa = 3.61VLYY405 pKa = 10.7YY406 pKa = 10.26KK407 pKa = 10.54VRR409 pKa = 11.84FMEE412 pKa = 4.93KK413 pKa = 10.46GLDD416 pKa = 3.41VCYY419 pKa = 10.95GNLPPAPQVNKK430 pKa = 10.32CNVWTFNDD438 pKa = 3.13ITFEE442 pKa = 4.18GVLLEE447 pKa = 4.28TNKK450 pKa = 9.29TFNPQLKK457 pKa = 9.64ALYY460 pKa = 8.51KK461 pKa = 10.09GSMVVMIRR469 pKa = 11.84VFGIVYY475 pKa = 9.41EE476 pKa = 4.03VVPCIKK482 pKa = 9.7TRR484 pKa = 11.84LSVIYY489 pKa = 10.14NANNKK494 pKa = 9.68NYY496 pKa = 7.97STLYY500 pKa = 10.25RR501 pKa = 11.84VANCNQIDD509 pKa = 4.24TKK511 pKa = 10.96RR512 pKa = 11.84SVHH515 pKa = 5.6PVGLLSRR522 pKa = 11.84DD523 pKa = 4.29GINTPCGCLRR533 pKa = 11.84NAMFSPNITVDD544 pKa = 3.33SYY546 pKa = 11.71SCKK549 pKa = 9.76TLLSDD554 pKa = 4.54GYY556 pKa = 11.17CVVFNNGSTEE566 pKa = 3.98VVPIGIVGANTTYY579 pKa = 11.35NMPILEE585 pKa = 4.15EE586 pKa = 4.35SFVEE590 pKa = 4.08LALDD594 pKa = 3.36HH595 pKa = 6.45VLVSEE600 pKa = 4.37SEE602 pKa = 4.35YY603 pKa = 10.71FQTEE607 pKa = 4.13MPVFDD612 pKa = 5.1IDD614 pKa = 3.84CEE616 pKa = 4.62KK617 pKa = 10.92YY618 pKa = 10.04ICDD621 pKa = 3.51NSEE624 pKa = 3.87SCRR627 pKa = 11.84MLLEE631 pKa = 4.19QYY633 pKa = 11.17AGFCSKK639 pKa = 9.12ITSDD643 pKa = 3.94VKK645 pKa = 11.31GNSQLLNANTLAMYY659 pKa = 10.66KK660 pKa = 10.4NIVPSDD666 pKa = 3.84SIKK669 pKa = 10.51PIEE672 pKa = 4.31NFGDD676 pKa = 3.92FNMSMFIDD684 pKa = 3.44KK685 pKa = 10.48SGRR688 pKa = 11.84SVLEE692 pKa = 4.02DD693 pKa = 3.53LLFDD697 pKa = 5.27KK698 pKa = 10.72IVTTGPGFYY707 pKa = 10.02EE708 pKa = 4.98DD709 pKa = 4.46YY710 pKa = 10.12YY711 pKa = 11.16KK712 pKa = 11.06CRR714 pKa = 11.84TTTVKK719 pKa = 10.8DD720 pKa = 3.65LVCAQYY726 pKa = 11.39YY727 pKa = 8.4NGIMIIPPIQDD738 pKa = 3.12AEE740 pKa = 4.49TIGMYY745 pKa = 10.51GGIVAAGMTMGLFGGQATMGTWQIAMSARR774 pKa = 11.84LNAVGVTQGMIMEE787 pKa = 5.23DD788 pKa = 3.64VQKK791 pKa = 10.72LANGFNTLATSVSKK805 pKa = 9.93MASVTADD812 pKa = 3.17ALSRR816 pKa = 11.84IQGVVNANALQVEE829 pKa = 4.62LLARR833 pKa = 11.84ATMDD837 pKa = 3.08NFGAISSNFEE847 pKa = 4.07IIEE850 pKa = 4.04KK851 pKa = 10.31RR852 pKa = 11.84LKK854 pKa = 9.94QVQAEE859 pKa = 4.09QQMDD863 pKa = 3.53RR864 pKa = 11.84VINGKK869 pKa = 8.63MNALLNFVSNYY880 pKa = 8.58KK881 pKa = 10.57LKK883 pKa = 10.72VSEE886 pKa = 4.3LTSIQQLVKK895 pKa = 10.8QKK897 pKa = 10.19VQEE900 pKa = 4.32CVHH903 pKa = 5.87AQSLRR908 pKa = 11.84NGFCGSGLHH917 pKa = 6.54VMSIPQLVPNGVMFLHH933 pKa = 7.11FSLVKK938 pKa = 10.26NNSIIVKK945 pKa = 7.12QTPGLCKK952 pKa = 9.73EE953 pKa = 4.58DD954 pKa = 4.44KK955 pKa = 10.48INCIVPKK962 pKa = 10.57SGIFVSKK969 pKa = 10.77NDD971 pKa = 3.83SNWYY975 pKa = 5.94ITPRR979 pKa = 11.84NKK981 pKa = 9.99YY982 pKa = 8.62VPKK985 pKa = 10.6NITNSDD991 pKa = 4.04IIFTTYY997 pKa = 10.73SEE999 pKa = 4.36NFTVINNTIQFPGIEE1014 pKa = 4.38FPQDD1018 pKa = 2.77GDD1020 pKa = 3.41FDD1022 pKa = 4.2GQIKK1026 pKa = 10.54NVTLEE1031 pKa = 4.07LQSLKK1036 pKa = 11.09DD1037 pKa = 3.43VVANMSYY1044 pKa = 11.41LDD1046 pKa = 4.25LSNHH1050 pKa = 5.2TALLKK1055 pKa = 10.88NISEE1059 pKa = 4.58EE1060 pKa = 4.19VKK1062 pKa = 10.74KK1063 pKa = 10.82MNITVSQFKK1072 pKa = 10.68QYY1074 pKa = 10.53VKK1076 pKa = 9.85YY1077 pKa = 10.88VKK1079 pKa = 9.66WPWYY1083 pKa = 8.28VWLLIVMALIAFGLVCFWIFMCTGCCGLCDD1113 pKa = 4.87CIRR1116 pKa = 11.84RR1117 pKa = 11.84SCCDD1121 pKa = 3.14SNTYY1125 pKa = 10.54EE1126 pKa = 4.81PIEE1129 pKa = 4.81KK1130 pKa = 9.87IHH1132 pKa = 5.99IQQ1134 pKa = 3.28

Molecular weight:
127.86 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A240FW20|A0A240FW20_9ALPC Nucleocapsid protein OS=Wencheng Sm shrew coronavirus OX=1508228 PE=4 SV=1
MM1 pKa = 7.21SAEE4 pKa = 4.18KK5 pKa = 10.79VSWSSSLPTKK15 pKa = 10.76ADD17 pKa = 3.29TKK19 pKa = 10.6NGSKK23 pKa = 10.57VEE25 pKa = 4.19VEE27 pKa = 4.21LSAWDD32 pKa = 4.38PIIIQNNKK40 pKa = 9.49NIQDD44 pKa = 3.75VLPRR48 pKa = 11.84TSVPKK53 pKa = 10.36GLPSGHH59 pKa = 6.89EE60 pKa = 3.01IGYY63 pKa = 8.73WYY65 pKa = 10.25KK66 pKa = 10.57GPEE69 pKa = 3.71RR70 pKa = 11.84YY71 pKa = 9.73RR72 pKa = 11.84FTRR75 pKa = 11.84GEE77 pKa = 4.25KK78 pKa = 10.68VPLDD82 pKa = 4.07PKK84 pKa = 10.2WYY86 pKa = 9.62FYY88 pKa = 10.77PLGHH92 pKa = 6.72GPAANKK98 pKa = 9.38KK99 pKa = 8.03WNSKK103 pKa = 9.7VEE105 pKa = 4.15GVFWVARR112 pKa = 11.84DD113 pKa = 3.43GASIEE118 pKa = 4.15KK119 pKa = 10.67VPDD122 pKa = 3.65FEE124 pKa = 4.43PRR126 pKa = 11.84KK127 pKa = 10.13KK128 pKa = 10.22GAEE131 pKa = 4.02AKK133 pKa = 9.89RR134 pKa = 11.84VRR136 pKa = 11.84LPGKK140 pKa = 10.31APANVYY146 pKa = 7.6FTTTSASRR154 pKa = 11.84SQSRR158 pKa = 11.84DD159 pKa = 2.72NSRR162 pKa = 11.84AASRR166 pKa = 11.84SGSKK170 pKa = 10.33SRR172 pKa = 11.84IQSRR176 pKa = 11.84SPSAEE181 pKa = 3.68RR182 pKa = 11.84GHH184 pKa = 8.04VDD186 pKa = 2.46IKK188 pKa = 11.11LAVLEE193 pKa = 4.05ALKK196 pKa = 10.87DD197 pKa = 3.66LGIGQDD203 pKa = 3.53TKK205 pKa = 11.27KK206 pKa = 10.55KK207 pKa = 9.22SSKK210 pKa = 10.21SAAASGNNTPKK221 pKa = 10.22QEE223 pKa = 4.34SSPKK227 pKa = 8.63VQRR230 pKa = 11.84KK231 pKa = 7.91QIEE234 pKa = 4.19RR235 pKa = 11.84LRR237 pKa = 11.84HH238 pKa = 4.91KK239 pKa = 10.31RR240 pKa = 11.84VPTGKK245 pKa = 10.41EE246 pKa = 3.68NITVCFGPRR255 pKa = 11.84GPEE258 pKa = 3.58QNFGSDD264 pKa = 3.54EE265 pKa = 4.12IVNGGAQGKK274 pKa = 9.16KK275 pKa = 8.73VAQLLEE281 pKa = 4.29NVPGPSALLFGGKK294 pKa = 9.2VEE296 pKa = 5.11QIGKK300 pKa = 10.29ANGKK304 pKa = 9.24VAVQYY309 pKa = 10.42TYY311 pKa = 11.2VAEE314 pKa = 4.57LDD316 pKa = 3.84DD317 pKa = 4.2NEE319 pKa = 4.27EE320 pKa = 4.01VKK322 pKa = 10.82RR323 pKa = 11.84FLDD326 pKa = 5.62LIDD329 pKa = 4.85AYY331 pKa = 10.01KK332 pKa = 10.69KK333 pKa = 8.49PTTANSINSVLNPQATAFAPPSGVIEE359 pKa = 4.17EE360 pKa = 4.77LQDD363 pKa = 4.05DD364 pKa = 4.5LNN366 pKa = 4.44

Molecular weight:
40.03 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

8128

75

6324

1625.6

183.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.798 ± 0.75

3.088 ± 0.665

5.376 ± 0.869

4.86 ± 0.415

6.115 ± 0.731

6.299 ± 0.424

1.513 ± 0.255

5.598 ± 0.831

7.16 ± 0.891

8.981 ± 0.715

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.387 ± 0.49

7.013 ± 0.574

2.879 ± 1.123

2.842 ± 0.479

3.027 ± 0.67

7.013 ± 0.665

4.737 ± 0.325

10.162 ± 0.954

1.28 ± 0.276

4.872 ± 0.488

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski