Cassava common mosaic virus
Average proteome isoelectric point is 6.82
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q65978|Q65978_9VIRU Coat protein OS=Cassava common mosaic virus OX=39046 PE=3 SV=1
MM1 pKa = 7.45 ATPTSTTPTTATITQAATTPLSALSTAPTDD31 pKa = 3.78 EE32 pKa = 4.19 EE33 pKa = 5.28 LSRR36 pKa = 11.84 LDD38 pKa = 5.07 LKK40 pKa = 10.71 PASNLVASADD50 pKa = 3.66 ALSAIAADD58 pKa = 3.66 WASLKK63 pKa = 10.84 VPTAQLMRR71 pKa = 11.84 HH72 pKa = 5.84 ALDD75 pKa = 3.84 LVNFCFDD82 pKa = 3.9 SGSSKK87 pKa = 9.28 YY88 pKa = 6.42 TTVEE92 pKa = 4.14 GSSPTPTIPRR102 pKa = 11.84 AALAGAVRR110 pKa = 11.84 KK111 pKa = 8.27 HH112 pKa = 3.55 TTLRR116 pKa = 11.84 QFCRR120 pKa = 11.84 YY121 pKa = 7.3 YY122 pKa = 11.42 AKK124 pKa = 10.32 IIWNARR130 pKa = 11.84 VKK132 pKa = 11.15 ANIPPAGYY140 pKa = 10.7 ANAHH144 pKa = 6.47 IKK146 pKa = 10.25 PEE148 pKa = 3.86 QAFAGFDD155 pKa = 3.58 FFDD158 pKa = 3.71 GVMNVAALEE167 pKa = 4.09 PSGGLVRR174 pKa = 11.84 EE175 pKa = 4.33 PTPQEE180 pKa = 3.71 IIAAEE185 pKa = 4.14 TARR188 pKa = 11.84 SLNLFEE194 pKa = 5.15 AQSKK198 pKa = 10.12 GNNLATNATQVTRR211 pKa = 11.84 GRR213 pKa = 11.84 LSSSEE218 pKa = 3.99 PQVQFLTGVDD228 pKa = 3.65 EE229 pKa = 4.44
Molecular weight: 24.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.033
IPC2_protein 5.995
IPC_protein 6.084
Toseland 6.389
ProMoST 6.02
Dawson 6.364
Bjellqvist 6.313
Wikipedia 6.364
Rodwell 6.338
Grimsley 6.504
Solomon 6.364
Lehninger 6.364
Nozaki 6.59
DTASelect 6.781
Thurlkill 6.795
EMBOSS 6.781
Sillero 6.722
Patrickios 4.507
IPC_peptide 6.389
IPC2_peptide 6.62
IPC2.peptide.svr19 6.681
Protein with the highest isoelectric point:
>tr|Q65977|Q65977_9VIRU Movement protein TGBp3 OS=Cassava common mosaic virus OX=39046 PE=3 SV=1
MM1 pKa = 7.56 SGHH4 pKa = 6.34 HH5 pKa = 6.78 HH6 pKa = 6.73 LTPPTDD12 pKa = 3.36 YY13 pKa = 11.28 SKK15 pKa = 11.51 AVLAAVIGISSSIFIHH31 pKa = 6.66 TITRR35 pKa = 11.84 STNPHH40 pKa = 5.62 VGDD43 pKa = 4.66 NIHH46 pKa = 6.73 ALPHH50 pKa = 5.36 GGYY53 pKa = 10.22 YY54 pKa = 9.63 RR55 pKa = 11.84 DD56 pKa = 3.77 GTKK59 pKa = 8.99 TVYY62 pKa = 10.69 YY63 pKa = 10.02 GGPGPTAVSASAGKK77 pKa = 9.73 FWAFLCIFTISGFLFFSNRR96 pKa = 11.84 HH97 pKa = 4.85 RR98 pKa = 11.84 TNSSCSCRR106 pKa = 11.84 PNLGANN112 pKa = 3.5
Molecular weight: 12.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.84
IPC2_protein 8.946
IPC_protein 9.063
Toseland 9.107
ProMoST 9.136
Dawson 9.472
Bjellqvist 9.458
Wikipedia 9.692
Rodwell 9.531
Grimsley 9.531
Solomon 9.633
Lehninger 9.589
Nozaki 9.487
DTASelect 9.341
Thurlkill 9.341
EMBOSS 9.575
Sillero 9.545
Patrickios 3.719
IPC_peptide 9.604
IPC2_peptide 8.726
IPC2.peptide.svr19 7.86
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
2118
97
1449
423.6
47.31
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.546 ± 1.472
1.228 ± 0.396
4.344 ± 0.456
6.468 ± 1.556
3.919 ± 0.259
5.241 ± 1.202
3.069 ± 0.688
5.996 ± 0.483
6.657 ± 1.572
10.67 ± 0.9
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.889 ± 0.562
3.683 ± 0.328
6.374 ± 0.574
4.438 ± 0.655
4.297 ± 0.298
7.224 ± 1.552
7.129 ± 1.03
4.485 ± 0.731
1.228 ± 0.272
3.116 ± 0.354
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here