Influenza A virus (A/chicken/Korea/S21/2004(H9N2))

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Insthoviricetes; Articulavirales; Orthomyxoviridae; Alphainfluenzavirus; Influenza A virus; H9N2 subtype

Average proteome isoelectric point is 7.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0S737|A0S737_9INFA Isoform of A0S738 Nuclear export protein OS=Influenza A virus (A/chicken/Korea/S21/2004(H9N2)) OX=526591 GN=NS PE=3 SV=1
MM1 pKa = 7.38SLLTEE6 pKa = 4.39VEE8 pKa = 4.23TPTRR12 pKa = 11.84KK13 pKa = 9.79GWEE16 pKa = 4.51CKK18 pKa = 10.37CSDD21 pKa = 3.73SSDD24 pKa = 3.78PLVIAASIIGILHH37 pKa = 7.1LILWILDD44 pKa = 3.42RR45 pKa = 11.84LLFKK49 pKa = 10.74CIYY52 pKa = 9.91RR53 pKa = 11.84RR54 pKa = 11.84LKK56 pKa = 10.55YY57 pKa = 10.03GLKK60 pKa = 10.09RR61 pKa = 11.84GPSTEE66 pKa = 3.92GMPEE70 pKa = 3.98SMRR73 pKa = 11.84EE74 pKa = 3.94EE75 pKa = 4.14YY76 pKa = 10.41RR77 pKa = 11.84QEE79 pKa = 3.74QQSAVDD85 pKa = 3.54VDD87 pKa = 4.34DD88 pKa = 4.18GG89 pKa = 4.24

Molecular weight:
10.19 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0S738|A0S738_9INFA Non-structural protein 1 OS=Influenza A virus (A/chicken/Korea/S21/2004(H9N2)) OX=526591 GN=NS PE=3 SV=1
MM1 pKa = 7.78ASQGTKK7 pKa = 9.71RR8 pKa = 11.84SYY10 pKa = 10.97EE11 pKa = 3.79QMEE14 pKa = 4.48TSGEE18 pKa = 4.06RR19 pKa = 11.84QNATEE24 pKa = 3.76IRR26 pKa = 11.84ASVGRR31 pKa = 11.84MVGGIGRR38 pKa = 11.84FYY40 pKa = 10.72IQMCTEE46 pKa = 4.88LKK48 pKa = 10.85LSDD51 pKa = 3.45HH52 pKa = 6.91EE53 pKa = 4.75GRR55 pKa = 11.84LIQNSITIEE64 pKa = 4.06RR65 pKa = 11.84MVLSAFDD72 pKa = 3.61EE73 pKa = 4.18RR74 pKa = 11.84RR75 pKa = 11.84NRR77 pKa = 11.84YY78 pKa = 9.39LEE80 pKa = 3.94EE81 pKa = 4.51HH82 pKa = 6.87PSAGKK87 pKa = 10.07DD88 pKa = 3.33PKK90 pKa = 10.14KK91 pKa = 9.42TGGPIYY97 pKa = 10.21RR98 pKa = 11.84RR99 pKa = 11.84RR100 pKa = 11.84DD101 pKa = 3.62GKK103 pKa = 8.92WVRR106 pKa = 11.84EE107 pKa = 4.0LILYY111 pKa = 10.04DD112 pKa = 3.4KK113 pKa = 11.39EE114 pKa = 3.93EE115 pKa = 3.69IRR117 pKa = 11.84RR118 pKa = 11.84IWRR121 pKa = 11.84QANNGEE127 pKa = 4.33DD128 pKa = 3.23ATAGLTHH135 pKa = 6.13MMIWHH140 pKa = 6.62SNLNDD145 pKa = 3.0ATYY148 pKa = 10.71QRR150 pKa = 11.84TRR152 pKa = 11.84ALVRR156 pKa = 11.84TGMDD160 pKa = 2.98PRR162 pKa = 11.84MCSLMQGSTLPRR174 pKa = 11.84RR175 pKa = 11.84SGAAGAAVKK184 pKa = 10.56GVGTMVMEE192 pKa = 5.61LIRR195 pKa = 11.84MIKK198 pKa = 10.22RR199 pKa = 11.84GVNDD203 pKa = 3.48RR204 pKa = 11.84NFWRR208 pKa = 11.84GEE210 pKa = 3.54NGRR213 pKa = 11.84RR214 pKa = 11.84TRR216 pKa = 11.84IAYY219 pKa = 9.45EE220 pKa = 3.95RR221 pKa = 11.84MCNILKK227 pKa = 10.75GKK229 pKa = 9.38FQTAAA234 pKa = 3.31

Molecular weight:
26.82 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

2

10

2556

89

406

255.6

28.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.908 ± 0.502

1.839 ± 0.348

4.382 ± 0.368

7.512 ± 0.972

3.443 ± 0.663

6.886 ± 0.633

1.956 ± 0.179

6.612 ± 0.522

5.477 ± 0.369

8.294 ± 0.508

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.834 ± 0.486

4.617 ± 0.542

3.795 ± 0.311

4.147 ± 0.422

7.394 ± 0.791

6.886 ± 0.407

7.629 ± 0.564

5.477 ± 0.439

1.487 ± 0.211

2.426 ± 0.35

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski