Influenza A virus (A/chicken/Korea/S21/2004(H9N2))
Average proteome isoelectric point is 7.02
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0S737|A0S737_9INFA Isoform of A0S738 Nuclear export protein OS=Influenza A virus (A/chicken/Korea/S21/2004(H9N2)) OX=526591 GN=NS PE=3 SV=1
MM1 pKa = 7.38 SLLTEE6 pKa = 4.39 VEE8 pKa = 4.23 TPTRR12 pKa = 11.84 KK13 pKa = 9.79 GWEE16 pKa = 4.51 CKK18 pKa = 10.37 CSDD21 pKa = 3.73 SSDD24 pKa = 3.78 PLVIAASIIGILHH37 pKa = 7.1 LILWILDD44 pKa = 3.42 RR45 pKa = 11.84 LLFKK49 pKa = 10.74 CIYY52 pKa = 9.91 RR53 pKa = 11.84 RR54 pKa = 11.84 LKK56 pKa = 10.55 YY57 pKa = 10.03 GLKK60 pKa = 10.09 RR61 pKa = 11.84 GPSTEE66 pKa = 3.92 GMPEE70 pKa = 3.98 SMRR73 pKa = 11.84 EE74 pKa = 3.94 EE75 pKa = 4.14 YY76 pKa = 10.41 RR77 pKa = 11.84 QEE79 pKa = 3.74 QQSAVDD85 pKa = 3.54 VDD87 pKa = 4.34 DD88 pKa = 4.18 GG89 pKa = 4.24
Molecular weight: 10.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.217
IPC2_protein 5.423
IPC_protein 5.232
Toseland 5.156
ProMoST 5.372
Dawson 5.219
Bjellqvist 5.347
Wikipedia 5.092
Rodwell 5.143
Grimsley 5.08
Solomon 5.219
Lehninger 5.169
Nozaki 5.347
DTASelect 5.474
Thurlkill 5.181
EMBOSS 5.13
Sillero 5.41
Patrickios 4.139
IPC_peptide 5.232
IPC2_peptide 5.41
IPC2.peptide.svr19 5.511
Protein with the highest isoelectric point:
>tr|A0S738|A0S738_9INFA Non-structural protein 1 OS=Influenza A virus (A/chicken/Korea/S21/2004(H9N2)) OX=526591 GN=NS PE=3 SV=1
MM1 pKa = 7.78 ASQGTKK7 pKa = 9.71 RR8 pKa = 11.84 SYY10 pKa = 10.97 EE11 pKa = 3.79 QMEE14 pKa = 4.48 TSGEE18 pKa = 4.06 RR19 pKa = 11.84 QNATEE24 pKa = 3.76 IRR26 pKa = 11.84 ASVGRR31 pKa = 11.84 MVGGIGRR38 pKa = 11.84 FYY40 pKa = 10.72 IQMCTEE46 pKa = 4.88 LKK48 pKa = 10.85 LSDD51 pKa = 3.45 HH52 pKa = 6.91 EE53 pKa = 4.75 GRR55 pKa = 11.84 LIQNSITIEE64 pKa = 4.06 RR65 pKa = 11.84 MVLSAFDD72 pKa = 3.61 EE73 pKa = 4.18 RR74 pKa = 11.84 RR75 pKa = 11.84 NRR77 pKa = 11.84 YY78 pKa = 9.39 LEE80 pKa = 3.94 EE81 pKa = 4.51 HH82 pKa = 6.87 PSAGKK87 pKa = 10.07 DD88 pKa = 3.33 PKK90 pKa = 10.14 KK91 pKa = 9.42 TGGPIYY97 pKa = 10.21 RR98 pKa = 11.84 RR99 pKa = 11.84 RR100 pKa = 11.84 DD101 pKa = 3.62 GKK103 pKa = 8.92 WVRR106 pKa = 11.84 EE107 pKa = 4.0 LILYY111 pKa = 10.04 DD112 pKa = 3.4 KK113 pKa = 11.39 EE114 pKa = 3.93 EE115 pKa = 3.69 IRR117 pKa = 11.84 RR118 pKa = 11.84 IWRR121 pKa = 11.84 QANNGEE127 pKa = 4.33 DD128 pKa = 3.23 ATAGLTHH135 pKa = 6.13 MMIWHH140 pKa = 6.62 SNLNDD145 pKa = 3.0 ATYY148 pKa = 10.71 QRR150 pKa = 11.84 TRR152 pKa = 11.84 ALVRR156 pKa = 11.84 TGMDD160 pKa = 2.98 PRR162 pKa = 11.84 MCSLMQGSTLPRR174 pKa = 11.84 RR175 pKa = 11.84 SGAAGAAVKK184 pKa = 10.56 GVGTMVMEE192 pKa = 5.61 LIRR195 pKa = 11.84 MIKK198 pKa = 10.22 RR199 pKa = 11.84 GVNDD203 pKa = 3.48 RR204 pKa = 11.84 NFWRR208 pKa = 11.84 GEE210 pKa = 3.54 NGRR213 pKa = 11.84 RR214 pKa = 11.84 TRR216 pKa = 11.84 IAYY219 pKa = 9.45 EE220 pKa = 3.95 RR221 pKa = 11.84 MCNILKK227 pKa = 10.75 GKK229 pKa = 9.38 FQTAAA234 pKa = 3.31
Molecular weight: 26.82 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.324
IPC2_protein 9.502
IPC_protein 10.175
Toseland 10.409
ProMoST 10.101
Dawson 10.555
Bjellqvist 10.262
Wikipedia 10.745
Rodwell 10.745
Grimsley 10.613
Solomon 10.628
Lehninger 10.599
Nozaki 10.409
DTASelect 10.248
Thurlkill 10.423
EMBOSS 10.804
Sillero 10.467
Patrickios 10.409
IPC_peptide 10.628
IPC2_peptide 9.209
IPC2.peptide.svr19 8.643
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8
2
10
2556
89
406
255.6
28.93
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.908 ± 0.502
1.839 ± 0.348
4.382 ± 0.368
7.512 ± 0.972
3.443 ± 0.663
6.886 ± 0.633
1.956 ± 0.179
6.612 ± 0.522
5.477 ± 0.369
8.294 ± 0.508
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.834 ± 0.486
4.617 ± 0.542
3.795 ± 0.311
4.147 ± 0.422
7.394 ± 0.791
6.886 ± 0.407
7.629 ± 0.564
5.477 ± 0.439
1.487 ± 0.211
2.426 ± 0.35
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here