Streptococcus phage CHPC1148

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Moineauvirus; unclassified Moineauvirus

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 50 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G8FB33|A0A3G8FB33_9CAUD DNA binding protein OS=Streptococcus phage CHPC1148 OX=2365028 GN=CHPC1148_0046 PE=4 SV=1
MM1 pKa = 7.31SVSKK5 pKa = 7.68TTIMQTLNLDD15 pKa = 3.8EE16 pKa = 4.97TDD18 pKa = 3.78DD19 pKa = 3.8TALIPAYY26 pKa = 9.69IEE28 pKa = 4.01SAKK31 pKa = 10.51QYY33 pKa = 10.21IINAVGSDD41 pKa = 3.23PKK43 pKa = 10.69FYY45 pKa = 10.59EE46 pKa = 4.26LEE48 pKa = 3.98NVEE51 pKa = 4.45YY52 pKa = 10.83LFDD55 pKa = 3.66TAVIALTSSYY65 pKa = 8.62FTYY68 pKa = 10.28RR69 pKa = 11.84VAITDD74 pKa = 3.55TATYY78 pKa = 9.07PINLTLNSIIGQLRR92 pKa = 11.84GLYY95 pKa = 8.4ATYY98 pKa = 10.9SEE100 pKa = 4.77VVTNGG105 pKa = 2.82

Molecular weight: 11.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G8FB61|A0A3G8FB61_9CAUD Terminase small subunit OS=Streptococcus phage CHPC1148 OX=2365028 GN=CHPC1148_0001 PE=4 SV=1
MM1 pKa = 7.36ARR3 pKa = 11.84VRR5 pKa = 11.84YY6 pKa = 9.32LPSDD10 pKa = 3.79FRR12 pKa = 11.84YY13 pKa = 9.99KK14 pKa = 10.95ADD16 pKa = 3.48FGTYY20 pKa = 9.38QSTPNKK26 pKa = 8.16FTGVSVPKK34 pKa = 10.14FVKK37 pKa = 10.38QFTLHH42 pKa = 5.9YY43 pKa = 9.16KK44 pKa = 9.46PHH46 pKa = 6.1TRR48 pKa = 11.84TLNQEE53 pKa = 3.69YY54 pKa = 10.23LAIQNGEE61 pKa = 3.55NDD63 pKa = 3.11TRR65 pKa = 11.84VIVIRR70 pKa = 11.84HH71 pKa = 4.8NAKK74 pKa = 9.68VLEE77 pKa = 4.46GQVVTLNGTQYY88 pKa = 11.15DD89 pKa = 3.86IVRR92 pKa = 11.84ISPDD96 pKa = 3.02EE97 pKa = 3.9NFGFNHH103 pKa = 6.62YY104 pKa = 11.07DD105 pKa = 3.14FLTLRR110 pKa = 11.84KK111 pKa = 9.07RR112 pKa = 11.84KK113 pKa = 9.7KK114 pKa = 10.3VGG116 pKa = 2.98

Molecular weight: 13.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

50

0

50

12027

44

1653

240.5

27.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.153 ± 0.524

0.657 ± 0.128

6.302 ± 0.208

6.826 ± 0.411

4.415 ± 0.198

6.577 ± 0.456

1.438 ± 0.117

6.918 ± 0.24

8.68 ± 0.499

7.999 ± 0.294

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.328 ± 0.14

6.078 ± 0.266

3.01 ± 0.17

3.733 ± 0.209

4.207 ± 0.257

6.66 ± 0.21

6.236 ± 0.303

6.161 ± 0.244

1.671 ± 0.155

3.949 ± 0.283
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski