West African Asystasia virus 1

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 7.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A190D9H7|A0A190D9H7_9GEMI AC5 OS=West African Asystasia virus 1 OX=1046573 PE=4 SV=1
MM1 pKa = 8.12WDD3 pKa = 3.43PLLNSFPPTIHH14 pKa = 7.19GFRR17 pKa = 11.84CMLALKK23 pKa = 10.45YY24 pKa = 10.82LLLLEE29 pKa = 4.91NNYY32 pKa = 10.2EE33 pKa = 4.22DD34 pKa = 4.24NSVGQVYY41 pKa = 9.85IRR43 pKa = 11.84EE44 pKa = 4.56LISVLRR50 pKa = 11.84AGDD53 pKa = 3.67YY54 pKa = 10.72VKK56 pKa = 10.91ASSRR60 pKa = 11.84YY61 pKa = 7.7CDD63 pKa = 4.2LYY65 pKa = 10.75PRR67 pKa = 11.84IQGTSPPEE75 pKa = 3.64LRR77 pKa = 11.84QPSCQCTKK85 pKa = 10.48CPRR88 pKa = 11.84HH89 pKa = 5.69QKK91 pKa = 10.77EE92 pKa = 4.38SMGEE96 pKa = 3.84QAHH99 pKa = 5.45VSEE102 pKa = 4.74TSSVPEE108 pKa = 4.4VYY110 pKa = 10.25QSS112 pKa = 2.97

Molecular weight: 12.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A190D9I4|A0A190D9I4_9GEMI AC4 OS=West African Asystasia virus 1 OX=1046573 PE=3 SV=1
MM1 pKa = 7.71SKK3 pKa = 10.35RR4 pKa = 11.84PADD7 pKa = 3.52IVISTPASKK16 pKa = 10.29VRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84LNFDD24 pKa = 3.26SPRR27 pKa = 11.84ASVPNVRR34 pKa = 11.84VTRR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84VWANRR44 pKa = 11.84PMYY47 pKa = 9.98RR48 pKa = 11.84KK49 pKa = 8.43PALYY53 pKa = 9.81RR54 pKa = 11.84KK55 pKa = 7.68YY56 pKa = 9.22TSRR59 pKa = 11.84DD60 pKa = 3.12VPRR63 pKa = 11.84GCEE66 pKa = 4.36GPCKK70 pKa = 10.33VQSFDD75 pKa = 3.23QRR77 pKa = 11.84DD78 pKa = 4.04DD79 pKa = 3.53IKK81 pKa = 10.95HH82 pKa = 6.1LGVVRR87 pKa = 11.84CISDD91 pKa = 3.36VTRR94 pKa = 11.84GPGITHH100 pKa = 6.76RR101 pKa = 11.84VGKK104 pKa = 9.62RR105 pKa = 11.84FCIKK109 pKa = 10.44SVLFTGKK116 pKa = 8.58IWMDD120 pKa = 3.46EE121 pKa = 4.13NIKK124 pKa = 10.05KK125 pKa = 10.0QNHH128 pKa = 4.27TNIVIFFLVRR138 pKa = 11.84DD139 pKa = 3.84RR140 pKa = 11.84RR141 pKa = 11.84PYY143 pKa = 10.9GSPQDD148 pKa = 3.87FGDD151 pKa = 3.79VFNMFDD157 pKa = 4.34NEE159 pKa = 4.11PSTATVKK166 pKa = 10.81NDD168 pKa = 2.84LRR170 pKa = 11.84DD171 pKa = 3.41RR172 pKa = 11.84YY173 pKa = 9.78QVLRR177 pKa = 11.84RR178 pKa = 11.84FTCSVTGGPSGCKK191 pKa = 8.82EE192 pKa = 3.67QALVRR197 pKa = 11.84RR198 pKa = 11.84FYY200 pKa = 10.55TINHH204 pKa = 5.37NVVYY208 pKa = 10.24NHH210 pKa = 5.98QEE212 pKa = 3.39AAKK215 pKa = 10.4YY216 pKa = 9.25EE217 pKa = 4.13NHH219 pKa = 6.46TEE221 pKa = 3.99NALLLYY227 pKa = 7.29MACTHH232 pKa = 7.07ASNPVYY238 pKa = 10.85ASMKK242 pKa = 8.99IRR244 pKa = 11.84IYY246 pKa = 10.61FYY248 pKa = 11.2DD249 pKa = 3.78SIGNN253 pKa = 3.69

Molecular weight: 29.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

0

9

1722

77

362

191.3

21.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.401 ± 0.592

2.091 ± 0.305

4.646 ± 0.382

4.588 ± 0.655

4.007 ± 0.437

5.168 ± 0.337

3.717 ± 0.618

6.446 ± 0.725

4.936 ± 0.623

6.794 ± 0.497

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.207 ± 0.266

5.459 ± 0.431

6.156 ± 0.859

4.239 ± 0.601

7.375 ± 1.001

9.466 ± 0.926

6.156 ± 0.831

5.807 ± 0.862

1.22 ± 0.228

4.123 ± 0.541
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski