Spiroplasma virus 4 (SpV4)

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; Gokushovirinae; Spiromicrovirus

Average proteome isoelectric point is 7.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P11336|H_SPV4 Minor spike protein H OS=Spiroplasma virus 4 OX=10855 GN=ORF4 PE=3 SV=1
MM1 pKa = 7.9KK2 pKa = 10.12IYY4 pKa = 8.2TQRR7 pKa = 11.84NNKK10 pKa = 9.27VEE12 pKa = 4.32FSDD15 pKa = 3.95SGSSEE20 pKa = 3.59YY21 pKa = 10.76SEE23 pKa = 4.18YY24 pKa = 10.84QRR26 pKa = 11.84VIDD29 pKa = 5.62ADD31 pKa = 3.92TKK33 pKa = 9.44EE34 pKa = 3.9NTYY37 pKa = 10.73EE38 pKa = 4.27IVATYY43 pKa = 10.74NRR45 pKa = 11.84YY46 pKa = 10.29DD47 pKa = 4.0EE48 pKa = 4.26IQEE51 pKa = 4.29AGEE54 pKa = 4.31GTDD57 pKa = 4.39LRR59 pKa = 11.84SMLDD63 pKa = 3.17KK64 pKa = 11.59YY65 pKa = 11.23GDD67 pKa = 4.25DD68 pKa = 3.94YY69 pKa = 12.13LEE71 pKa = 4.57LLPPARR77 pKa = 11.84LGGDD81 pKa = 3.4DD82 pKa = 4.09TILPKK87 pKa = 10.51SVLEE91 pKa = 4.19LEE93 pKa = 4.67NIRR96 pKa = 11.84LQNTEE101 pKa = 4.11YY102 pKa = 11.13LSLLEE107 pKa = 4.86NINSKK112 pKa = 10.5LDD114 pKa = 3.51KK115 pKa = 10.83QGLGDD120 pKa = 4.44LDD122 pKa = 4.29NFIKK126 pKa = 10.38NWQEE130 pKa = 3.81SQKK133 pKa = 10.84KK134 pKa = 9.47IEE136 pKa = 4.03NEE138 pKa = 3.75KK139 pKa = 10.66GKK141 pKa = 10.96KK142 pKa = 8.86EE143 pKa = 3.91DD144 pKa = 3.6EE145 pKa = 4.58KK146 pKa = 11.46EE147 pKa = 4.02NEE149 pKa = 3.93

Molecular weight: 17.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P11341|ORF9_SPV4 Uncharacterized protein ORF9 OS=Spiroplasma virus 4 OX=10855 GN=ORF9 PE=4 SV=1
MM1 pKa = 7.37RR2 pKa = 11.84RR3 pKa = 11.84KK4 pKa = 9.48VKK6 pKa = 8.61NTKK9 pKa = 7.87RR10 pKa = 11.84HH11 pKa = 4.16QWRR14 pKa = 11.84LTHH17 pKa = 5.87SARR20 pKa = 11.84SIKK23 pKa = 9.9RR24 pKa = 11.84ANIMPSNPRR33 pKa = 11.84GGRR36 pKa = 11.84RR37 pKa = 11.84FF38 pKa = 3.34

Molecular weight: 4.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

0

9

1423

28

553

158.1

18.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.06 ± 1.12

1.054 ± 0.483

5.481 ± 0.91

5.341 ± 1.231

3.935 ± 0.558

6.325 ± 0.966

2.178 ± 0.681

6.676 ± 1.244

8.573 ± 1.38

8.714 ± 1.197

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.881 ± 0.708

5.762 ± 0.488

3.935 ± 1.1

3.865 ± 0.564

6.395 ± 1.828

7.027 ± 0.844

4.919 ± 0.764

5.903 ± 0.869

1.827 ± 0.366

4.146 ± 0.889
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski