Helobdella robusta (Californian leech)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Spiralia; Lophotrochozoa; Annelida; Clitellata; Hirudinea; Rhynchobdellida; Glossiphoniidae; Helobdella

Average proteome isoelectric point is 6.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 23328 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|T1F5M2|T1F5M2_HELRO Apple domain-containing protein OS=Helobdella robusta OX=6412 GN=20204121 PE=4 SV=1
MM1 pKa = 6.91STSDD5 pKa = 3.44VKK7 pKa = 10.96RR8 pKa = 11.84EE9 pKa = 3.63EE10 pKa = 3.7LAQYY14 pKa = 10.75VEE16 pKa = 4.37EE17 pKa = 4.45EE18 pKa = 4.39KK19 pKa = 10.72PATEE23 pKa = 4.78LNLGMYY29 pKa = 8.42MCDD32 pKa = 2.79WEE34 pKa = 4.53FGINGYY40 pKa = 7.38TLFRR44 pKa = 11.84KK45 pKa = 9.29NRR47 pKa = 11.84EE48 pKa = 3.73YY49 pKa = 11.06QNNHH53 pKa = 3.76GHH55 pKa = 5.91EE56 pKa = 4.1AVAVNLFVNNNMNVNIVFLNPCTPDD81 pKa = 3.39FSGVNLKK88 pKa = 9.92IGKK91 pKa = 9.09KK92 pKa = 10.17CVISTNGWYY101 pKa = 10.57VDD103 pKa = 3.65MFKK106 pKa = 11.07DD107 pKa = 5.18DD108 pKa = 3.87NNLDD112 pKa = 3.42KK113 pKa = 11.18RR114 pKa = 11.84GNTSIMHH121 pKa = 6.64GCPDD125 pKa = 3.59TEE127 pKa = 4.34VRR129 pKa = 11.84KK130 pKa = 10.39CLDD133 pKa = 4.18DD134 pKa = 4.02EE135 pKa = 5.94DD136 pKa = 4.62GTEE139 pKa = 4.04STNYY143 pKa = 10.59DD144 pKa = 3.44NNDD147 pKa = 3.35YY148 pKa = 11.49DD149 pKa = 3.99SGNDD153 pKa = 3.33IDD155 pKa = 5.77DD156 pKa = 3.84NHH158 pKa = 7.29NGDD161 pKa = 5.06DD162 pKa = 4.5NEE164 pKa = 3.93KK165 pKa = 10.68DD166 pKa = 3.91YY167 pKa = 11.9YY168 pKa = 10.61NEE170 pKa = 3.95EE171 pKa = 4.43YY172 pKa = 11.06VNKK175 pKa = 10.45DD176 pKa = 3.52YY177 pKa = 11.37FNEE180 pKa = 4.46DD181 pKa = 4.14YY182 pKa = 11.54VNKK185 pKa = 10.62DD186 pKa = 3.0YY187 pKa = 11.65DD188 pKa = 4.38NEE190 pKa = 4.19DD191 pKa = 4.06DD192 pKa = 5.35YY193 pKa = 12.24DD194 pKa = 5.68NEE196 pKa = 5.58DD197 pKa = 3.9DD198 pKa = 5.5DD199 pKa = 6.37NNVDD203 pKa = 3.82YY204 pKa = 11.38DD205 pKa = 3.83NEE207 pKa = 4.29EE208 pKa = 4.03YY209 pKa = 10.94DD210 pKa = 5.34DD211 pKa = 5.13NDD213 pKa = 2.71VDD215 pKa = 4.39YY216 pKa = 11.64YY217 pKa = 11.39NEE219 pKa = 4.32EE220 pKa = 4.21YY221 pKa = 10.84DD222 pKa = 5.8DD223 pKa = 4.14EE224 pKa = 7.53DD225 pKa = 4.67YY226 pKa = 11.74DD227 pKa = 4.94NEE229 pKa = 4.87DD230 pKa = 4.21DD231 pKa = 4.91VDD233 pKa = 4.21VDD235 pKa = 4.55DD236 pKa = 4.63EE237 pKa = 4.81TMIQAKK243 pKa = 9.91KK244 pKa = 8.08LAKK247 pKa = 9.6MIAGVVDD254 pKa = 5.01EE255 pKa = 4.81KK256 pKa = 11.65

Molecular weight: 29.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|T1G9Q4|T1G9Q4_HELRO Uncharacterized protein OS=Helobdella robusta OX=6412 GN=20217800 PE=3 SV=1
RR1 pKa = 6.87QRR3 pKa = 11.84LMLWSRR9 pKa = 11.84QRR11 pKa = 11.84LMLWPRR17 pKa = 11.84QRR19 pKa = 11.84LMLWSRR25 pKa = 11.84QRR27 pKa = 11.84LMLWSRR33 pKa = 11.84QRR35 pKa = 11.84LMLWSRR41 pKa = 11.84QRR43 pKa = 11.84LMLWSRR49 pKa = 11.84QRR51 pKa = 11.84LMLWSRR57 pKa = 11.84QRR59 pKa = 11.84LL60 pKa = 3.49

Molecular weight: 8.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

23328

0

23328

8768599

49

6265

375.9

42.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.14 ± 0.014

2.321 ± 0.014

5.943 ± 0.02

5.635 ± 0.019

4.285 ± 0.013

4.867 ± 0.027

2.516 ± 0.01

6.001 ± 0.014

7.129 ± 0.02

8.77 ± 0.02

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.359 ± 0.008

7.516 ± 0.039

3.958 ± 0.014

4.291 ± 0.02

4.536 ± 0.013

8.829 ± 0.026

5.747 ± 0.016

5.957 ± 0.015

1.004 ± 0.006

3.195 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski