Canis familiaris papillomavirus 2
Average proteome isoelectric point is 6.25
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q647I1|Q647I1_9PAPI Protein E6 OS=Canis familiaris papillomavirus 2 OX=292792 GN=E6 PE=3 SV=1
MM1 pKa = 7.73 RR2 pKa = 11.84 GSSPIIRR9 pKa = 11.84 DD10 pKa = 2.73 IDD12 pKa = 3.83 LEE14 pKa = 4.26 LEE16 pKa = 4.24 EE17 pKa = 5.38 LVLPANLLSGEE28 pKa = 4.16 TLEE31 pKa = 4.39 TEE33 pKa = 4.15 EE34 pKa = 5.5 EE35 pKa = 4.08 EE36 pKa = 4.42 LQRR39 pKa = 11.84 EE40 pKa = 4.23 PGRR43 pKa = 11.84 YY44 pKa = 8.22 RR45 pKa = 11.84 VVTLCNICHH54 pKa = 6.02 SSLRR58 pKa = 11.84 LFVEE62 pKa = 4.47 VADD65 pKa = 4.31 EE66 pKa = 4.03 SLIRR70 pKa = 11.84 LFQQLLLDD78 pKa = 4.0 GLGIICATCHH88 pKa = 6.56 KK89 pKa = 10.12 EE90 pKa = 3.8 HH91 pKa = 6.88 FSDD94 pKa = 3.74 GRR96 pKa = 11.84 RR97 pKa = 11.84 RR98 pKa = 3.42
Molecular weight: 11.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.876
IPC2_protein 4.927
IPC_protein 4.787
Toseland 4.698
ProMoST 4.889
Dawson 4.736
Bjellqvist 4.876
Wikipedia 4.571
Rodwell 4.66
Grimsley 4.609
Solomon 4.736
Lehninger 4.685
Nozaki 4.851
DTASelect 4.94
Thurlkill 4.685
EMBOSS 4.596
Sillero 4.927
Patrickios 3.961
IPC_peptide 4.749
IPC2_peptide 4.927
IPC2.peptide.svr19 4.891
Protein with the highest isoelectric point:
>tr|Q647H9|Q647H9_9PAPI Replication protein E1 OS=Canis familiaris papillomavirus 2 OX=292792 GN=E1 PE=3 SV=1
MM1 pKa = 7.56 LEE3 pKa = 3.76 SLARR7 pKa = 11.84 RR8 pKa = 11.84 FDD10 pKa = 3.61 VLQEE14 pKa = 4.12 VMLHH18 pKa = 6.22 HH19 pKa = 6.59 YY20 pKa = 10.23 EE21 pKa = 4.25 SGSKK25 pKa = 10.55 KK26 pKa = 9.87 LTDD29 pKa = 3.21 QCNYY33 pKa = 8.66 WEE35 pKa = 4.16 AARR38 pKa = 11.84 RR39 pKa = 11.84 EE40 pKa = 4.35 SALLHH45 pKa = 5.18 YY46 pKa = 10.22 ARR48 pKa = 11.84 KK49 pKa = 9.78 NRR51 pKa = 11.84 ITRR54 pKa = 11.84 LGITPVPSLATSEE67 pKa = 4.31 NSAKK71 pKa = 10.39 KK72 pKa = 10.06 AIRR75 pKa = 11.84 MSLLLSSLCNSPYY88 pKa = 10.24 KK89 pKa = 10.47 DD90 pKa = 4.25 EE91 pKa = 4.64 PWTLADD97 pKa = 3.56 TSIEE101 pKa = 3.93 LWEE104 pKa = 5.01 CPPKK108 pKa = 11.06 GCFKK112 pKa = 10.9 KK113 pKa = 10.85 GGFTVDD119 pKa = 3.23 VLFDD123 pKa = 4.04 NDD125 pKa = 3.83 PGNVFPYY132 pKa = 9.12 TAWAYY137 pKa = 9.81 IYY139 pKa = 9.67 YY140 pKa = 10.24 QNQNDD145 pKa = 3.52 EE146 pKa = 4.5 WIKK149 pKa = 10.83 VPGQVDD155 pKa = 3.92 YY156 pKa = 11.68 DD157 pKa = 3.74 GLFYY161 pKa = 10.68 TDD163 pKa = 3.45 EE164 pKa = 5.82 DD165 pKa = 4.25 GEE167 pKa = 4.14 KK168 pKa = 10.0 RR169 pKa = 11.84 YY170 pKa = 10.12 YY171 pKa = 8.82 QTFAKK176 pKa = 10.41 DD177 pKa = 3.22 ALRR180 pKa = 11.84 FGTTGMWKK188 pKa = 9.19 VCYY191 pKa = 9.32 KK192 pKa = 10.5 HH193 pKa = 6.57 HH194 pKa = 6.45 VLSALISSSGSSNSAASSSWQSNIEE219 pKa = 4.16 SSSGDD224 pKa = 3.02 QWSAQWQWEE233 pKa = 4.62 GPTGSSASSTAASVSSSSTCSRR255 pKa = 11.84 TGGTRR260 pKa = 11.84 EE261 pKa = 3.92 AGGSFDD267 pKa = 4.87 QGGSSQAGGAEE278 pKa = 4.04 RR279 pKa = 11.84 GRR281 pKa = 11.84 GRR283 pKa = 11.84 GRR285 pKa = 11.84 GRR287 pKa = 11.84 GRR289 pKa = 11.84 GRR291 pKa = 11.84 GRR293 pKa = 11.84 GRR295 pKa = 11.84 ASSGSGSGSGSTSRR309 pKa = 11.84 SGSRR313 pKa = 11.84 SDD315 pKa = 3.56 SVSGSGGAKK324 pKa = 9.08 PVYY327 pKa = 8.28 PQPRR331 pKa = 11.84 VGGNKK336 pKa = 9.67 APQKK340 pKa = 9.52 RR341 pKa = 11.84 QRR343 pKa = 11.84 EE344 pKa = 4.33 APSDD348 pKa = 3.87 TEE350 pKa = 3.9 DD351 pKa = 3.22 LPRR354 pKa = 11.84 NRR356 pKa = 11.84 GVLGRR361 pKa = 11.84 GRR363 pKa = 11.84 GASPSTWSSNVRR375 pKa = 11.84 NIPPNTPTIPTLSTPGPCPGDD396 pKa = 3.15 RR397 pKa = 11.84 AGVGRR402 pKa = 11.84 GGGPRR407 pKa = 11.84 EE408 pKa = 3.99 PKK410 pKa = 10.49 APRR413 pKa = 11.84 LSPAVPLGTVGRR425 pKa = 11.84 RR426 pKa = 11.84 PGEE429 pKa = 3.84 PQEE432 pKa = 4.16 PLEE435 pKa = 4.39 PEE437 pKa = 4.05 PGRR440 pKa = 11.84 SQEE443 pKa = 3.92 QNRR446 pKa = 11.84 PFPVILVKK454 pKa = 10.93 GDD456 pKa = 3.54 GNASKK461 pKa = 10.44 CWRR464 pKa = 11.84 RR465 pKa = 11.84 RR466 pKa = 11.84 LRR468 pKa = 11.84 LRR470 pKa = 11.84 YY471 pKa = 9.49 SGLFEE476 pKa = 4.86 SISTGFSWTTASGPHH491 pKa = 5.7 RR492 pKa = 11.84 VCRR495 pKa = 11.84 PRR497 pKa = 11.84 MLIAFNSDD505 pKa = 2.84 GQRR508 pKa = 11.84 DD509 pKa = 3.79 HH510 pKa = 6.78 FLKK513 pKa = 10.7 NIKK516 pKa = 9.37 IPKK519 pKa = 9.25 VLDD522 pKa = 3.12 IALGSFDD529 pKa = 4.16 SLL531 pKa = 4.03
Molecular weight: 57.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.983
IPC2_protein 9.077
IPC_protein 9.121
Toseland 9.706
ProMoST 9.502
Dawson 9.97
Bjellqvist 9.721
Wikipedia 10.16
Rodwell 10.204
Grimsley 10.058
Solomon 10.014
Lehninger 9.97
Nozaki 9.838
DTASelect 9.677
Thurlkill 9.823
EMBOSS 10.145
Sillero 9.926
Patrickios 5.575
IPC_peptide 9.999
IPC2_peptide 8.595
IPC2.peptide.svr19 8.009
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8
0
8
2754
41
607
344.3
38.13
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.483 ± 0.668
2.687 ± 0.99
5.556 ± 0.372
6.173 ± 0.51
4.357 ± 0.571
8.315 ± 1.132
1.961 ± 0.327
4.103 ± 0.637
4.466 ± 0.648
9.005 ± 0.912
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.743 ± 0.423
4.031 ± 0.6
7.734 ± 1.497
3.595 ± 0.326
6.572 ± 0.816
8.533 ± 1.247
5.81 ± 0.844
5.846 ± 0.767
1.38 ± 0.36
2.651 ± 0.408
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here