Cupriavidus sp. USMAA2-4

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae; Cupriavidus; unclassified Cupriavidus

Average proteome isoelectric point is 6.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7301 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1D9H4J6|A0A1D9H4J6_9BURK Cytochrome ubiquinol oxidase subunit I OS=Cupriavidus sp. USMAA2-4 OX=876364 GN=BKK79_22665 PE=3 SV=1
MM1 pKa = 7.64IPPASVKK8 pKa = 10.31HH9 pKa = 5.17GQEE12 pKa = 3.67PTLQQDD18 pKa = 3.36DD19 pKa = 3.78AVIYY23 pKa = 7.48KK24 pKa = 8.53TWVCLICGWVYY35 pKa = 11.37DD36 pKa = 4.56EE37 pKa = 4.48AQGWPEE43 pKa = 4.39DD44 pKa = 4.82DD45 pKa = 3.52IAPGTRR51 pKa = 11.84WEE53 pKa = 5.63DD54 pKa = 3.33IPDD57 pKa = 3.4DD58 pKa = 4.01WRR60 pKa = 11.84CPEE63 pKa = 4.37CDD65 pKa = 3.0VGKK68 pKa = 10.73ADD70 pKa = 4.17FAMIEE75 pKa = 3.97II76 pKa = 4.71

Molecular weight: 8.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1D9H094|A0A1D9H094_9BURK Uncharacterized protein OS=Cupriavidus sp. USMAA2-4 OX=876364 GN=BKK79_16500 PE=4 SV=1
MM1 pKa = 7.19LTDD4 pKa = 3.9ALLAFLHH11 pKa = 6.08FTAIFALITFLAAEE25 pKa = 4.23AVVLRR30 pKa = 11.84PGLTPAALKK39 pKa = 10.47RR40 pKa = 11.84LSVYY44 pKa = 10.8DD45 pKa = 4.27LLYY48 pKa = 10.17FLCAMLVLASGLLRR62 pKa = 11.84LFFGAKK68 pKa = 9.49GAGFYY73 pKa = 10.97LPNPWFHH80 pKa = 7.27AKK82 pKa = 7.93MTVFVVIALCSLPPTRR98 pKa = 11.84AFSRR102 pKa = 11.84WRR104 pKa = 11.84KK105 pKa = 8.37RR106 pKa = 11.84ARR108 pKa = 11.84SEE110 pKa = 3.93PGFVPAEE117 pKa = 4.24AEE119 pKa = 4.11LKK121 pKa = 10.13RR122 pKa = 11.84ARR124 pKa = 11.84RR125 pKa = 11.84WVMIEE130 pKa = 3.49AHH132 pKa = 6.71LLILLPLCAVMMARR146 pKa = 11.84GIGIKK151 pKa = 10.16

Molecular weight: 16.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7301

0

7301

2332933

29

4500

319.5

34.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.098 ± 0.047

0.94 ± 0.011

5.142 ± 0.022

5.101 ± 0.026

3.387 ± 0.02

8.827 ± 0.039

2.214 ± 0.016

4.074 ± 0.023

2.616 ± 0.025

10.875 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.324 ± 0.013

2.34 ± 0.021

5.436 ± 0.022

3.85 ± 0.023

7.503 ± 0.033

5.138 ± 0.023

4.908 ± 0.024

7.481 ± 0.024

1.415 ± 0.011

2.329 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski