Trichomonas vaginalis virus 3
Average proteome isoelectric point is 5.96
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q8V616|Q8V616_9VIRU Capsid protein OS=Trichomonas vaginalis virus 3 OX=170965 GN=cap PE=4 SV=1
MM1 pKa = 7.71 DD2 pKa = 4.73 HH3 pKa = 6.88 VSDD6 pKa = 3.39 IAYY9 pKa = 10.5 LNFLRR14 pKa = 11.84 WVLLPYY20 pKa = 10.33 NGQTLRR26 pKa = 11.84 PHH28 pKa = 6.37 PSVWRR33 pKa = 11.84 QTPYY37 pKa = 10.41 PEE39 pKa = 4.17 HH40 pKa = 6.9 VNLKK44 pKa = 9.82 FLNKK48 pKa = 9.83 EE49 pKa = 3.83 MEE51 pKa = 4.46 LEE53 pKa = 4.05 LFPLKK58 pKa = 10.34 KK59 pKa = 10.48 APQADD64 pKa = 3.8 LKK66 pKa = 10.02 VNCYY70 pKa = 9.75 ARR72 pKa = 11.84 NVLASTEE79 pKa = 4.1 LTDD82 pKa = 5.96 DD83 pKa = 4.45 LLKK86 pKa = 10.78 QSLPIGLNNDD96 pKa = 3.75 SVCGIVIVLEE106 pKa = 4.0 LLRR109 pKa = 11.84 IAGVPSKK116 pKa = 10.22 LLPIIGQAIANKK128 pKa = 10.45 DD129 pKa = 3.56 PFIKK133 pKa = 10.35 EE134 pKa = 3.7 LSDD137 pKa = 3.55 FNKK140 pKa = 10.19 MIGATTSRR148 pKa = 11.84 IANILTEE155 pKa = 4.37 CNTLIGRR162 pKa = 11.84 GVKK165 pKa = 10.35 SSDD168 pKa = 3.13 PSADD172 pKa = 3.1 LYY174 pKa = 11.37 HH175 pKa = 6.94 RR176 pKa = 11.84 VAPEE180 pKa = 4.07 GNRR183 pKa = 11.84 HH184 pKa = 4.74 EE185 pKa = 4.79 AKK187 pKa = 10.1 IPRR190 pKa = 11.84 HH191 pKa = 5.28 ILIEE195 pKa = 4.73 AINKK199 pKa = 9.11 IYY201 pKa = 10.19 KK202 pKa = 10.2 NEE204 pKa = 4.04 MTDD207 pKa = 3.39 MPPPGDD213 pKa = 3.73 FKK215 pKa = 11.52 LHH217 pKa = 7.23 LITSPLWCKK226 pKa = 10.39 AGSHH230 pKa = 5.76 HH231 pKa = 6.8 HH232 pKa = 5.98 PHH234 pKa = 5.93 FAKK237 pKa = 10.6 YY238 pKa = 10.19 SSRR241 pKa = 11.84 LDD243 pKa = 3.54 FVMDD247 pKa = 3.83 VPADD251 pKa = 4.04 KK252 pKa = 10.51 IAAVPPSVFITQAEE266 pKa = 4.22 KK267 pKa = 11.08 LEE269 pKa = 4.45 HH270 pKa = 6.31 GKK272 pKa = 7.86 TRR274 pKa = 11.84 YY275 pKa = 9.27 IYY277 pKa = 10.73 NCDD280 pKa = 2.94 TXSYY284 pKa = 10.86 LFFDD288 pKa = 4.72 YY289 pKa = 10.4 ILHH292 pKa = 5.57 YY293 pKa = 9.68 VEE295 pKa = 5.77 CVWSNEE301 pKa = 4.08 SVLLNPAAMSVEE313 pKa = 4.15 RR314 pKa = 11.84 FSVLDD319 pKa = 3.52 YY320 pKa = 11.16 PEE322 pKa = 4.68 YY323 pKa = 11.39 CMIDD327 pKa = 3.35 YY328 pKa = 10.75 TDD330 pKa = 4.64 FNSQHH335 pKa = 6.02 SLEE338 pKa = 4.24 SQKK341 pKa = 11.06 LVFEE345 pKa = 4.52 CLRR348 pKa = 11.84 PYY350 pKa = 10.46 LPSEE354 pKa = 3.78 MHH356 pKa = 7.5 PILDD360 pKa = 3.53 WCIASMDD367 pKa = 3.45 HH368 pKa = 6.37 MEE370 pKa = 5.08 IGGQHH375 pKa = 6.29 WLSTLPSGHH384 pKa = 7.24 RR385 pKa = 11.84 ATTFINSVLNKK396 pKa = 10.14 AYY398 pKa = 10.37 LIPYY402 pKa = 9.55 IGDD405 pKa = 3.36 TTSFHH410 pKa = 6.98 CGDD413 pKa = 4.64 DD414 pKa = 3.49 VLLCGKK420 pKa = 10.09 YY421 pKa = 10.12 DD422 pKa = 3.77 YY423 pKa = 9.52 QTLIDD428 pKa = 3.92 TLPYY432 pKa = 10.15 EE433 pKa = 4.39 LNKK436 pKa = 10.4 SKK438 pKa = 11.0 QSFGPNAEE446 pKa = 3.89 FLRR449 pKa = 11.84 LHH451 pKa = 6.49 RR452 pKa = 11.84 RR453 pKa = 11.84 AGDD456 pKa = 3.48 VIGYY460 pKa = 7.13 PSRR463 pKa = 11.84 AVSSLVSGNWLSKK476 pKa = 9.65 TSWEE480 pKa = 4.11 WQPSLISVTNQCNVIISRR498 pKa = 11.84 SQLNIRR504 pKa = 11.84 FIPAMQQEE512 pKa = 4.32 LRR514 pKa = 11.84 NRR516 pKa = 11.84 YY517 pKa = 7.74 IDD519 pKa = 3.86 KK520 pKa = 10.07 MSEE523 pKa = 4.02 PFDD526 pKa = 3.8 VGSDD530 pKa = 3.98 YY531 pKa = 11.52 YY532 pKa = 11.78 VMPGCPCYY540 pKa = 10.7 SDD542 pKa = 3.74 AATTIVPNVPQLEE555 pKa = 4.38 CSDD558 pKa = 4.0 VPFSQAQKK566 pKa = 10.74 VFDD569 pKa = 4.09 TMRR572 pKa = 11.84 DD573 pKa = 2.99 ICPEE577 pKa = 3.87 FTTVNDD583 pKa = 3.63 VIDD586 pKa = 3.74 RR587 pKa = 11.84 VLARR591 pKa = 11.84 RR592 pKa = 11.84 TSNAVKK598 pKa = 10.39 NITYY602 pKa = 9.33 NVCAPVAPQVCIAVNPAHH620 pKa = 5.92 YY621 pKa = 10.32 QFLLRR626 pKa = 11.84 KK627 pKa = 8.91 KK628 pKa = 9.1 YY629 pKa = 10.5 YY630 pKa = 8.88 PRR632 pKa = 11.84 EE633 pKa = 4.34 HH634 pKa = 6.86 IAPPGFDD641 pKa = 4.46 DD642 pKa = 3.78 STNSKK647 pKa = 10.55 LVFSTYY653 pKa = 11.3 DD654 pKa = 3.65 LAPSIAMKK662 pKa = 10.29 SCAVLTPAKK671 pKa = 9.59 IICGHH676 pKa = 5.96 GLRR679 pKa = 11.84 SGG681 pKa = 3.43
Molecular weight: 76.8 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.943
IPC2_protein 6.02
IPC_protein 6.097
Toseland 6.389
ProMoST 6.517
Dawson 6.453
Bjellqvist 6.453
Wikipedia 6.478
Rodwell 6.453
Grimsley 6.517
Solomon 6.453
Lehninger 6.453
Nozaki 6.751
DTASelect 6.912
Thurlkill 6.942
EMBOSS 6.912
Sillero 6.854
Patrickios 3.592
IPC_peptide 6.465
IPC2_peptide 6.766
IPC2.peptide.svr19 6.726
Protein with the highest isoelectric point:
>tr|Q8V616|Q8V616_9VIRU Capsid protein OS=Trichomonas vaginalis virus 3 OX=170965 GN=cap PE=4 SV=1
MM1 pKa = 7.64 SAPEE5 pKa = 3.95 PLNTEE10 pKa = 3.76 VRR12 pKa = 11.84 SPNGVSEE19 pKa = 5.13 AIEE22 pKa = 4.22 TQNMAVTQSSVSNEE36 pKa = 3.81 IKK38 pKa = 10.69 NDD40 pKa = 3.7 TQSDD44 pKa = 4.17 LQTLKK49 pKa = 10.89 KK50 pKa = 10.1 QLQPLYY56 pKa = 10.49 RR57 pKa = 11.84 STDD60 pKa = 3.42 FNTLYY65 pKa = 11.07 NFFYY69 pKa = 10.84 GLDD72 pKa = 3.68 VPASTDD78 pKa = 3.36 RR79 pKa = 11.84 VGHH82 pKa = 6.72 AIQRR86 pKa = 11.84 NTSVNDD92 pKa = 3.54 TNEE95 pKa = 4.11 VVSFPLTATVSHH107 pKa = 6.81 TFSNTPVPANIQPLQISVADD127 pKa = 3.86 DD128 pKa = 3.54 SVNYY132 pKa = 10.03 EE133 pKa = 3.98 LDD135 pKa = 3.46 EE136 pKa = 5.04 SGTLCPTLDD145 pKa = 3.53 SSVHH149 pKa = 4.13 VQRR152 pKa = 11.84 ATSLASALKK161 pKa = 10.46 VKK163 pKa = 9.49 LTGEE167 pKa = 4.13 IMHH170 pKa = 6.82 SDD172 pKa = 3.07 SVRR175 pKa = 11.84 PVQTPQLIAYY185 pKa = 8.11 LFGVLLGVKK194 pKa = 10.02 DD195 pKa = 3.44 RR196 pKa = 11.84 VNIHH200 pKa = 6.66 RR201 pKa = 11.84 NQPTNLWRR209 pKa = 11.84 SLCSPGRR216 pKa = 11.84 AAQAKK221 pKa = 9.32 PFFDD225 pKa = 3.64 EE226 pKa = 4.63 FPNNKK231 pKa = 8.84 FRR233 pKa = 11.84 TGALLAPPLPDD244 pKa = 4.04 AGFGPFPAEE253 pKa = 4.19 GLNQNSKK260 pKa = 11.14 LDD262 pKa = 3.85 FRR264 pKa = 11.84 SKK266 pKa = 11.0 GYY268 pKa = 10.62 VFYY271 pKa = 10.75 KK272 pKa = 10.02 QRR274 pKa = 11.84 TYY276 pKa = 11.54 NPDD279 pKa = 2.84 EE280 pKa = 4.22 MNRR283 pKa = 11.84 AFWFLWAIYY292 pKa = 10.74 NRR294 pKa = 11.84 MPEE297 pKa = 4.43 DD298 pKa = 3.51 FQLSYY303 pKa = 10.62 PLNITFCTSEE313 pKa = 4.99 LPVQNPMPGADD324 pKa = 3.74 AISNEE329 pKa = 4.08 QCEE332 pKa = 4.37 KK333 pKa = 11.24 ALLLLEE339 pKa = 4.32 KK340 pKa = 10.74 VVLEE344 pKa = 4.41 FFNNDD349 pKa = 2.57 RR350 pKa = 11.84 KK351 pKa = 10.16 LAYY354 pKa = 9.58 YY355 pKa = 10.1 YY356 pKa = 10.73 VFKK359 pKa = 10.89 GCQFVMRR366 pKa = 11.84 PCSCYY371 pKa = 10.16 QEE373 pKa = 4.78 GGLIRR378 pKa = 11.84 KK379 pKa = 9.32 ASRR382 pKa = 11.84 NVALRR387 pKa = 11.84 GFTGIYY393 pKa = 9.4 YY394 pKa = 10.72 LAGFTDD400 pKa = 4.1 QYY402 pKa = 11.72 ANMISCAVHH411 pKa = 6.09 PGVVGALFQYY421 pKa = 10.39 VDD423 pKa = 3.74 TMVLQAVFSLSGPKK437 pKa = 9.76 LVRR440 pKa = 11.84 FAAPPEE446 pKa = 4.07 YY447 pKa = 9.62 QGRR450 pKa = 11.84 HH451 pKa = 4.7 ACPFSFVADD460 pKa = 3.73 EE461 pKa = 4.67 NYY463 pKa = 9.99 WGIAPGIEE471 pKa = 3.95 AEE473 pKa = 4.23 PVGMYY478 pKa = 10.92 YY479 pKa = 9.76 MDD481 pKa = 5.05 IIQRR485 pKa = 11.84 KK486 pKa = 8.9 AEE488 pKa = 4.0 HH489 pKa = 7.02 DD490 pKa = 3.84 LFIEE494 pKa = 4.3 TFMDD498 pKa = 3.63 IYY500 pKa = 11.01 GSTASIICANIEE512 pKa = 4.06 TSLFTSGTNVLNHH525 pKa = 6.67 RR526 pKa = 11.84 MQKK529 pKa = 10.59 DD530 pKa = 3.92 FARR533 pKa = 11.84 DD534 pKa = 3.49 TPKK537 pKa = 10.66 PGTLRR542 pKa = 11.84 HH543 pKa = 4.59 QHH545 pKa = 6.22 AIINRR550 pKa = 11.84 FHH552 pKa = 6.51 EE553 pKa = 4.14 PEE555 pKa = 3.61 YY556 pKa = 10.65 AYY558 pKa = 10.98 RR559 pKa = 11.84 LGILANGVIPLSGSFEE575 pKa = 3.98 VDD577 pKa = 2.52 ILKK580 pKa = 10.3 EE581 pKa = 4.05 AEE583 pKa = 4.13 LLVTGEE589 pKa = 5.29 DD590 pKa = 3.36 IRR592 pKa = 11.84 NLPGLRR598 pKa = 11.84 CLCTRR603 pKa = 11.84 GLDD606 pKa = 3.89 AILGLRR612 pKa = 11.84 PVQQKK617 pKa = 9.66 RR618 pKa = 11.84 KK619 pKa = 8.44 KK620 pKa = 7.93 MCYY623 pKa = 9.74 FRR625 pKa = 11.84 TLDD628 pKa = 3.76 GNFHH632 pKa = 6.62 EE633 pKa = 4.59 VTIRR637 pKa = 11.84 SEE639 pKa = 4.14 TRR641 pKa = 11.84 DD642 pKa = 3.48 LQVWRR647 pKa = 11.84 DD648 pKa = 3.47 HH649 pKa = 6.8 GYY651 pKa = 10.0 LARR654 pKa = 11.84 PYY656 pKa = 9.41 ACHH659 pKa = 6.93 IIDD662 pKa = 3.63 SEE664 pKa = 4.63 GIEE667 pKa = 5.36 FYY669 pKa = 10.94 DD670 pKa = 4.99 KK671 pKa = 11.71 SNGLYY676 pKa = 10.01 KK677 pKa = 10.83 GRR679 pKa = 11.84 VNVLVSGFAIPGRR692 pKa = 11.84 AYY694 pKa = 10.04 QGPRR698 pKa = 11.84 LQVATMAAQII708 pKa = 3.98
Molecular weight: 79.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.987
IPC2_protein 6.059
IPC_protein 6.122
Toseland 6.376
ProMoST 6.532
Dawson 6.453
Bjellqvist 6.453
Wikipedia 6.465
Rodwell 6.453
Grimsley 6.491
Solomon 6.465
Lehninger 6.453
Nozaki 6.737
DTASelect 6.898
Thurlkill 6.927
EMBOSS 6.898
Sillero 6.839
Patrickios 3.872
IPC_peptide 6.478
IPC2_peptide 6.751
IPC2.peptide.svr19 6.71
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
1389
681
708
694.5
78.09
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.983 ± 0.448
2.448 ± 0.328
5.4 ± 0.318
4.752 ± 0.232
4.68 ± 0.577
5.256 ± 0.767
2.736 ± 0.43
5.976 ± 0.621
4.32 ± 0.451
9.431 ± 0.174
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.232 ± 0.079
5.4 ± 0.174
6.695 ± 0.335
3.96 ± 0.587
5.112 ± 0.473
7.127 ± 0.537
5.256 ± 0.275
6.695 ± 0.059
1.008 ± 0.21
4.464 ± 0.059
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here