Acidovorax phage ACP17
Average proteome isoelectric point is 6.16
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 253 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A218M2T3|A0A218M2T3_9CAUD Uncharacterized protein OS=Acidovorax phage ACP17 OX=2010329 PE=4 SV=1
MM1 pKa = 7.57 SSNNGASTILGITAGLGSVDD21 pKa = 4.18 FVFDD25 pKa = 3.98 KK26 pKa = 10.92 KK27 pKa = 10.82 ALQIVPPVDD36 pKa = 4.68 FIFGADD42 pKa = 3.01 KK43 pKa = 11.01 CFPDD47 pKa = 4.43 WMDD50 pKa = 3.11 QSGGSIIVDD59 pKa = 2.8 IDD61 pKa = 3.77 GCCFDD66 pKa = 4.81 VANDD70 pKa = 3.8 WVSVAGDD77 pKa = 4.41 SIVLDD82 pKa = 3.98 ATKK85 pKa = 10.43 GWQDD89 pKa = 2.93 ASGDD93 pKa = 3.8 QLVLDD98 pKa = 4.1 NGKK101 pKa = 9.71 SAVCPPDD108 pKa = 3.4 WEE110 pKa = 4.36 PQSGGSIDD118 pKa = 3.48 VDD120 pKa = 4.73 FYY122 pKa = 11.72 CCTTGGGEE130 pKa = 4.68 ALPIDD135 pKa = 4.5 LGAVRR140 pKa = 11.84 FTEE143 pKa = 4.38 GSVFVADD150 pKa = 5.75 AITDD154 pKa = 3.64 PRR156 pKa = 11.84 ISLGFYY162 pKa = 10.12 EE163 pKa = 4.99 GATFEE168 pKa = 4.62 ADD170 pKa = 3.25 LVRR173 pKa = 11.84 YY174 pKa = 8.84 PSVLLSADD182 pKa = 3.46 AVGGEE187 pKa = 4.28 YY188 pKa = 10.63 VEE190 pKa = 4.35 ASIYY194 pKa = 10.01 TDD196 pKa = 3.18 VVLPADD202 pKa = 3.94 AAAGEE207 pKa = 4.16 YY208 pKa = 10.83 LEE210 pKa = 5.41 LALAVFPAIDD220 pKa = 3.88 LQAVAVDD227 pKa = 4.36 GASLEE232 pKa = 4.25 AKK234 pKa = 10.51 VEE236 pKa = 4.23 FTTGFPQTYY245 pKa = 8.07 TQGEE249 pKa = 4.3 WAVADD254 pKa = 4.42 LEE256 pKa = 4.31 ITPYY260 pKa = 10.72 EE261 pKa = 4.0 GLIPVAHH268 pKa = 6.46 TGEE271 pKa = 4.15 VLQVSVEE278 pKa = 4.43 TITGFQVPVYY288 pKa = 9.99 VGEE291 pKa = 4.26 SADD294 pKa = 3.57 ASLASTTTFDD304 pKa = 4.12 ANAHH308 pKa = 5.27 TGEE311 pKa = 4.22 NASADD316 pKa = 3.93 LAVAPPAYY324 pKa = 9.21 LTAEE328 pKa = 4.43 LATGEE333 pKa = 4.42 SFVASLALGASSFGVNFAHH352 pKa = 6.96 EE353 pKa = 4.43 GSEE356 pKa = 4.48 AYY358 pKa = 10.49 VSLDD362 pKa = 3.28 TRR364 pKa = 11.84 PAADD368 pKa = 5.1 LFWQFYY374 pKa = 10.29 AGEE377 pKa = 4.2 QGSVTLSLGANLGEE391 pKa = 4.06 VGFFEE396 pKa = 4.82 GVRR399 pKa = 11.84 AEE401 pKa = 4.02 ASLGEE406 pKa = 4.33 EE407 pKa = 3.91 PNVYY411 pKa = 10.23 AFHH414 pKa = 6.89 GEE416 pKa = 4.07 VMDD419 pKa = 3.77 WALRR423 pKa = 11.84 TEE425 pKa = 4.92 DD426 pKa = 3.64 GFQAGVSQGEE436 pKa = 4.5 SASLEE441 pKa = 4.09 LNVRR445 pKa = 11.84 VSEE448 pKa = 4.26 GLGEE452 pKa = 3.99 LAAADD457 pKa = 4.08 GSTVFANLGALKK469 pKa = 10.25 AIPLAVTFYY478 pKa = 11.29 NSYY481 pKa = 8.82 TVHH484 pKa = 7.16 SGIGASTFLDD494 pKa = 3.96 LDD496 pKa = 3.88 SGCASGHH503 pKa = 5.8 NPGKK507 pKa = 10.28 QDD509 pKa = 2.82 IHH511 pKa = 8.1 RR512 pKa = 11.84 IEE514 pKa = 4.26 MNDD517 pKa = 3.98 AEE519 pKa = 5.14 FPDD522 pKa = 3.59 QRR524 pKa = 11.84 FDD526 pKa = 3.76 GDD528 pKa = 3.93 RR529 pKa = 11.84 TCVSLDD535 pKa = 3.7 LSAQPRR541 pKa = 11.84 FSFNFFGGEE550 pKa = 3.94 TFRR553 pKa = 11.84 TVDD556 pKa = 3.25 AADD559 pKa = 3.74 PSLRR563 pKa = 11.84 TVLSNEE569 pKa = 3.75 GATMRR574 pKa = 11.84 FALEE578 pKa = 3.82 SNINFRR584 pKa = 11.84 LCPGNFIPDD593 pKa = 3.41 GDD595 pKa = 3.91 NVYY598 pKa = 10.77 VEE600 pKa = 5.29 LDD602 pKa = 3.55 VEE604 pKa = 5.06 DD605 pKa = 4.23 VGNCQSDD612 pKa = 4.36 YY613 pKa = 11.34 AFHH616 pKa = 6.53 GVNVSAWLQANQNFQPVGHH635 pKa = 6.7 TGEE638 pKa = 4.37 TLTFDD643 pKa = 3.69 LTTAEE648 pKa = 4.26 LWTLVGQAGEE658 pKa = 4.35 TFSMASQPDD667 pKa = 3.99 PQPRR671 pKa = 11.84 AYY673 pKa = 10.18 EE674 pKa = 4.24 GVRR677 pKa = 11.84 VDD679 pKa = 3.21 ATFYY683 pKa = 10.37 EE684 pKa = 4.47 PPIVGAEE691 pKa = 4.06 GATAEE696 pKa = 4.07 ASLTIKK702 pKa = 11.08 YY703 pKa = 8.09 EE704 pKa = 4.1 VKK706 pKa = 10.27 FDD708 pKa = 4.45 EE709 pKa = 5.24 VGCLDD714 pKa = 5.71 NEE716 pKa = 5.3 YY717 pKa = 10.06 IWQDD721 pKa = 3.58 DD722 pKa = 3.74 NGDD725 pKa = 4.5 PITEE729 pKa = 4.5 LFNPVAVEE737 pKa = 3.94 MDD739 pKa = 4.13 PFSHH743 pKa = 8.03 DD744 pKa = 2.9 IRR746 pKa = 11.84 AHH748 pKa = 5.96 CEE750 pKa = 3.48
Molecular weight: 79.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.707
IPC2_protein 3.783
IPC_protein 3.808
Toseland 3.592
ProMoST 3.948
Dawson 3.783
Bjellqvist 3.948
Wikipedia 3.694
Rodwell 3.63
Grimsley 3.503
Solomon 3.783
Lehninger 3.745
Nozaki 3.884
DTASelect 4.113
Thurlkill 3.63
EMBOSS 3.706
Sillero 3.923
Patrickios 0.985
IPC_peptide 3.783
IPC2_peptide 3.91
IPC2.peptide.svr19 3.823
Protein with the highest isoelectric point:
>tr|A0A218M3A9|A0A218M3A9_9CAUD Uncharacterized protein OS=Acidovorax phage ACP17 OX=2010329 PE=4 SV=1
MM1 pKa = 7.92 AKK3 pKa = 9.03 FTSLNTQVYY12 pKa = 9.79 IPDD15 pKa = 3.46 NFAPLGKK22 pKa = 9.59 EE23 pKa = 3.55 KK24 pKa = 10.5 KK25 pKa = 10.56 GYY27 pKa = 10.12 FVDD30 pKa = 3.83 LGDD33 pKa = 4.05 RR34 pKa = 11.84 SVHH37 pKa = 5.82 SIHH40 pKa = 7.58 RR41 pKa = 11.84 GGCLKK46 pKa = 10.82 ALTPYY51 pKa = 9.96 KK52 pKa = 10.38 GRR54 pKa = 11.84 YY55 pKa = 7.94 KK56 pKa = 10.54 LRR58 pKa = 11.84 PAAGGWLARR67 pKa = 11.84 DD68 pKa = 4.4 VIAKK72 pKa = 9.94 AKK74 pKa = 8.4 RR75 pKa = 11.84 TSKK78 pKa = 9.17 TPRR81 pKa = 11.84 FARR84 pKa = 11.84 VV85 pKa = 2.74
Molecular weight: 9.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.295
IPC2_protein 9.926
IPC_protein 10.438
Toseland 10.774
ProMoST 10.365
Dawson 10.877
Bjellqvist 10.526
Wikipedia 11.038
Rodwell 11.33
Grimsley 10.921
Solomon 10.935
Lehninger 10.921
Nozaki 10.745
DTASelect 10.526
Thurlkill 10.76
EMBOSS 11.169
Sillero 10.789
Patrickios 11.067
IPC_peptide 10.95
IPC2_peptide 9.282
IPC2.peptide.svr19 8.506
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
253
0
253
48500
40
1903
191.7
21.34
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.532 ± 0.241
1.157 ± 0.072
6.161 ± 0.13
6.66 ± 0.168
4.365 ± 0.124
7.237 ± 0.191
1.944 ± 0.095
5.062 ± 0.119
5.757 ± 0.19
7.668 ± 0.137
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.773 ± 0.096
4.054 ± 0.124
4.235 ± 0.106
3.449 ± 0.097
5.416 ± 0.137
6.167 ± 0.164
5.852 ± 0.172
7.437 ± 0.159
1.596 ± 0.083
3.478 ± 0.109
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here